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Company Profile
Applied Biosystems, the company that enabled the sequencing of the human genome, brings an unmatched depth and breadth of products, knowledge and experience to every area of genetic discovery. In addition to leading edge, high-performance instruments, our integrated solutions include validated, genome-wide assays; application-specific software; industry-standard reagents; and responsive, knowledgeable applications consulting, support, and technical service. We offer the industry's most comprehensive and integrated portfolio of life science tools.


Real-Time PCR
Applied Biosystems pioneered the development of real-time PCR in 1995, enabling high-throughput, quantitative gene expression. Today, Applied Biosystems instruments and TaqMan®Assays  offer industry leading performance for Gene Expression, miRNA profiling, SNP Genotyping, Copy Number Variation and Drug Metabolism Enzyme evaluation.

To learn more about our simple, affordable approach to real-time PCR and gene expression analysis, please select one of our featured products from the list below.


TaqMan Gene Expression Assays, miRNA Assays and TaqMan Array For a comprehensive collection of information related to TaqMan Gene Expression Assays please visit www.allgenes.com
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Real-Time PCR Instruments

NEW StepOne™ Real-time PCR System
The StepOne™ Real-Time PCR System combines plug and play convenience, uncompromising performance and Fast high speed thermal cycling, that is tailor-made for both first-time users and real-time PCR experts.

7300 Real-Time PCR System
The Applied Biosystems 7300 Real-Time PCR System gives you advanced, multicolor real-time PCR capabilities with exceptional budget sensitivity.

7500 Real-Time PCR System
The Applied Biosystems 7500 Real-Time PCR System is a leading edge system with features to support labs requiring more capabilities from their real-time PCR platform.

7500 Fast Real-Time PCR System
The Applied Biosystems 7500 Fast Real-Time PCR System enables high speed thermal cycling in a 96-well format, reducing your run times to less than 40 minutes and accelerating your research by providing access to results more quickly than ever before.

7900HT Fast Real-Time PCR System
The Applied Biosystems 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading - and now also offers optional Fast real-time PCR capability.


Assays & Arrays
TaqMan® Gene Expression Assays
Which gene expression assay is right for you? Use our (Gene Assist only offers TaqMan assays relative to pathways in the tool rather than all assays we offer) Gene Expression Assay web search site (Gene Expression Assays) to find the right TaqMan® Assay for your needs.
TaqMan® Gene Expression Assays are a comprehensive collection of over 700,000 predesigned probe and primer sets for quantitative gene expression analysis of human, mouse, rat, Arabidopsis, Drosophila, Caenorhabditis elegans and Rhesus Macaque genes. These assays use universal reaction conditions to let you quickly, easily, and affordably perform precise gene expression studies using real-time PCR.


Custom TaqMan® Gene Expression Assays
Order custom TaqMan® assays to perform gene expression studies on any gene or splice variant in any organism. Get faster, more reproducible results by submitting your sequence and we will design the assay that works under universal reaction conditions.

TaqMan® MicroRNA Assays
Choose from >850  TaqMan real-time PCR-based microRNA assays, listed by the species they interrogate: Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Human, Mouse, Rat. Controls function across all species.
You have the option to search for assays by assay name, part number, target sequence, miRBase name, miRBase accession number, and target species.

TaqMan® Low Density Arrays
The TaqMan® arrays are customized 384-well micro fluidic cards. Choose from over 40,000 assays to custom configure your own TaqMan array for human, mouse, and rat genes. The pre-loaded arrays are easy to use, eliminate the need for robotics, and provide a standardized robust approach to gene signature experiments.  Alternatively, select from pre-defined gene signature panels defined by experts covering druggable, disease and gene pathway gene sets. See list TaqMan® Low Density Arrays for current list of panels, gene lists and TaqMan array layout.

SNP Genotyping Assays:

TaqMan® SNP Genotyping Assays
With over 4.5 million SNP assays available, including 2.7 million HapMap SNPs, and 60K cSNPs, Applied Biosystems SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry. These assays provide SNP detection capabilities with consistently high-confidence results.

Custom TaqMan® SNP Genotyping Assays
Order custom, single tube TaqMan® reagent-based assays to perform genotyping studies with any possible SNP in any organism. Custom TaqMan® SNP Genotyping Assays support both SNPs and insertion-deletions of up to six bases.

TaqMan® Drug Metabolism Genotyping Assays
Genotype over 2,400 high value polymorphisms located in regulatory elements and coding regions for 220 drug metabolism and transporter genes.

TaqMan® Pre-Developed Assay Reagents for Allelic Discrimination
(TaqMan® PDARs for AD)

TaqMan® Pre-Developed Assay Reagents for Allelic Discrimination (TaqMan® PDARs for AD) use the 5' nuclease assay to genotype purified DNA samples for specific mutations. Most TaqMan® PDARs for AD assays discriminate between two alleles of single nucleotide polymorphisms (SNPs).


Primers & Probes Custom Probes (Labeled Primers):
  • TaqMan® MGB™ Probes are dual-labeled custom probes that can be used in all of your real-time and end-point PCR applications.
  • TaqMan® TAMRA™ Probes are dual labeled, real-time PCR custom probes that can be used in all of your real-time PCR applications.
Custom Unlabeled Primers:
  • Sequence Detection Primers are unlabeled, real-time PCR primers that can be used with TaqMan® Probes in all of your real-time PCR applications.


Enzymes & Kits
Applied Biosystems offers a whole range of enzymes and kits for your real-time PCR needs, such as cDNA Synthesis Kits, Hot-Start Kits, Master Mixes, RT-PCR Kits & Reagents, RT-PCR Master Mixes, and more.


TaqMan® Universal PCR Master Mix
This kit contains components needed for all 5' nuclease assays using TaqMan®-probe PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Power SYBR® Green PCR Master Mix
Obtain superior sensitivity and reproducibility without compromising any performance parameters in your real-time, quantitative PCR experiments.

New! TaqMan® PreAmp Master Mix Kit (Early Access)
TaqMan® PreAmp Master Mix Kit (Early Access) preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your limited sample into many more real-time PCR reactions.



Reaction Plates, Adhesive Films & Accessories

Tubes, Caps, & Accessories
Which reaction plates, tubes, and adhesive films can you use with your real-time PCR instrument?
Read our Plastic Consumables Compatibility Chart (PDF, 40 KB).


Useful links

www.allgenes.com

Quantitative Analysis of the PCR Reaction

Creating Standard Curves for Use in Quantitative PCR

Technical Support Tools & Tutorials

2006 PCR & Real-Time PCR Consumables Catalogue


News & Events Visit our magazine “Innovations” for a full list of special offers on genomic products.


AB-Online
Opt-in to receive useful information tailored to your needs via e-mail.


Webinars
Stay informed on all the newest innovations and technical know-how.
Register for an upcoming webinar or view a recorded webinar.


Contact To learn more Contact your local sales representatives

Contact:
Applied Biosystems
Applera Deutschland GmbH
Frankfurter Straße 129 B
64293 Darmstadt
Germany

Inhouse Sales: Contact Kai-Uwe Brodersen 49 (0) 6151 9670-5371
Phone:     49 6151 9670 0 
Fax:         49 6151 9670 5599 
E-mail: germany.order@eur.appliedbiosystems.com

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