The Complete Solution for Real-Time PCR



www.agilent.com/genomics	Technology Designed for You Exceptional qPCR requires more than just great technology — it requires tools that are specifically designed to optimize your work, combined with the expert service and support you need to ensure your success. Agilent Technologies offers qPCR products engineered to simplify and improve your sample preparation, assay setup, and data analysis. Featuring a proven and reliable platform, industry leading support, and a catalog of superior reagents — including our new line of Brilliant III reagents which does not sacrifice sensitivity or specificity even when performing cycling under fast ramping conditions — Agilent is committed to providing the best qPCR experience available.

	Mx Family of qPCR Systems Tools You Can Rely On Cited in more than 3000 publications, the Agilent Mx3005P and Mx3000P QPCR Systems are the most flexible — and reliable — instruments available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Agilent’s qPCR software, MxPro, provides users with an intuitive interface, quick experiment design, powerful data analysis and easy report generation. All of these features and more make qPCR with the Mx instrument an exciting and dependable user experience. Proven Leaders in Flexibility and Performance The Mx3005P and Mx3000P QPCR Systems are among the most reliable — and trusted — instruments available, with a long record of citations in peer-reviewed journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries. Highly-reproducible results are the product of the Mx3005P and Mx3000P’s single-light source, single-detector precision optic scanning design providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy. The advanced optical system used by both the Mx3005P and Mx3000P offers numerous key benefits. Eliminates optical variation by ensuring uniform excitation intensity and emission measurement from well-to-well, across the entire plate. Elimination of optical variation comes from the single-scanning fiber optic head design that ensures: Each well receives the same amount of excitation light Each well receives excitation light for the same length of time Each well is the same distance from the detector Eliminates the need for well-to-well signal correction by calibration or reference dyes. Uniformity of well-to-well measurements from the single-scanning fiber optic head design reduces the need for additional signal correction and calibration steps, streamlining your assay. Allows accurate quantification of low- to high-abundance targets. Use of a single photomultiplier tube (PMT) that provides linear detection over ten orders of magnitude ensures superior sensitivity and reproducibility. Increases your flexibility. Has four (Mx3000P) or five (Mx3005P) optical channels with user-selected filters that cover a broad range of excitation wavelengths, allowing use with most fluorescent dyes. Mx3005P QPCR System—Unmatched Flexibility The well-equipped Mx3005P QPCR System’s five-position user customizable filter wheel design allows you to choose which five filters are installed in your instrument from a list of eight filter sets spanning deep blue dyes to far red dyes. This flexibility enables: Multiplex dye combinations with up to five targets per well Custom excitation and emission filter pairs to accommodate the rapidly-expanding list of fluorescent dyes Most fluorescent dye chemistries for FRET or other applications: • Ability to mismatch excitation and emission filters • Large Stokes shift dyes Numerous applications your future research may need More information Mx3000P QPCR System — Full-Featured Performance The Mx3000P is a high-performance yet low-cost option designed to accommodate basic to advanced experimental applications. Featuring four optical channels with user-selected filters covering a broad range of excitation wavelengths, the Mx3000P can be used with most fluorescent dyes, bringing flexible real-time qPCR instrumentation to the individual researcher with a limited budget. More information MxPro QPCR Software — Designed for Ease of Use and Powerful Data Analysis Agilent’s MxPro qPCR software incorporates innovative data analysis and intuitive design for the ultimate ease of use. From one-click plate setup to custom report generation, MxPro software is designed to help you move your research further with ease. At the same time, the software also has many advanced features that will allow you to grow with the software and experience all of the power MxPro has to offer. Analyze up to 12 separate plates at once — Multiple Experiment Analysis functionality allows the user to analyze a single experiment that spans several plates simultaneously Easily customize each experiment • Input your own well names • Assign your own assay or gene target name to each dye • Work with a flexible thermal profile setup View and analyze data in real time Choose between two options for setting baseline subtraction and thresholds when analyzing data. For example, the “Adaptive Baseline” algorithm calculates baseline start and end cycles independently for each amplification plot Create custom reports — plots, charts, and labels can be exported directly to Microsoft applications

	MxPro ET MxPro is 21 CFR Part 11 compatible, complete with secure application login, database file management, and data file audit trails. The audit trail records changes made to the data, and reports can be generated for the audit trail, user accounts, and error logs. More information

	Amplification Reagents Agilent offers a complete portfolio of ready-to-use reagents for all your real-time PCR applications. Your qPCR needs are dependent upon your application, your sample — RNA, DNA, or cDNA — and your real-time PCR instrument. All our qPCR reagents are optimized for superior sensitivity; delivering exceptional quantification and reproducibility. Our range of Brilliant qPCR reagents offers you the flexibility to amplify and quantify different templates and targets on almost any real-time PCR system. Click on the links below to see data and learn more about each specific qPCR reagent. Click here for a detailed selection guide to our qPCR product portfolio.
	Probe-based qPCR and qRT-PCR Reagents Brilliant III Ultra-Fast QPCR & QRT-PCR Reagents Delivers industry leading sensitivity, specificity, and reproducibility on fast-cyclig real-time PCR instruments Novel fast Taq mutant and enhanced rapid hot start capability delivers qPCR results in under 40 minutes and reduces primer-dimer formation with a novel hot-start technology Convenient pre-blended formulations compatible with any sequence-specific probe detection chemistry Perform duplex amplification in a single reaction Extremely sensitive detection (1 µg – 10 fg) of input cDNA More information Brilliant II Probe-Based QPCR and QRT-PCR Reagents Greater flexibility for use with different templates and targets More accurate quantification results Available in 1-step and 2-step formats (uses AffinityScript Multiple Temperature Reverse Transcriptase) Extremely sensitive detection (1 µg – 10 fg) of input cDNA More Information Brilliant II QPCR & QRT-PCR Reagents w/ROX Validated for qPCR with probes on all ROX-dependent real-time PCR systems Formulations available with low and high concentrations of ROX reference dye Improved performance with earlier Ct detection across a wide dynamic range Increased sensitivity of detection (1 µg – 0.1 pg) of input RNA More Information Brilliant II FAST QPCR Reagents Use with fluorescent probes to amplify and detect a variety of DNA targets, including genomic DNA, plasmid DNA, and cDNA Accelerated cycling protocol that completes 40 cycles of PCR in under 50 minutes Rapid hot-start capability to minimize non-specific product formation while reducing run time Improved performance with earlier Ct detection across a wide dynamic range delivers increased sensitivity of detection down to low copy numbers More Information
	SYBR® Green-based QPCR & QRT PCR Reagents Brilliant III Ultra-Fast SYBR® Green QPCR & QRT-PCR Reagents Designed specifically for qPCR with SYBR® Green on fast-cycling real-time PCR instruments Novel fast Taq mutant for qPCR results in minutes Sensitively and accurately detects low-abundance targets (specific amplification over at least 7 orders of magnitude) Enhanced rapid hot start capability saves time and reduces primer-dimer formation Optimized fast-cycling formulation ensures reliable and reproducible data with shorter run times Convenient pre-blended formulations compatible with any SYBR Green dye More Information Brilliant II SYBR® Green QPCR and QRT-PCR Reagents Formulated to deliver improved performance and reproducibility with cDNA, genomic DNA, and plasmid DNA templates over a broad dynamic range More accurate quantification results Developed on the Agilent Mx QPCR Systems More Information Brilliant II SYBR® Green QPCR and QRT-PCR w/ROX Reagents Validated for qPCR with SYBR® Green on all ROX-dependent real-time PCR systems Pre-blended master mixes containing low or high concentrations of ROX reference dye Improved performance with earlier Ct detection across a wide dynamic range Increased sensitivity of detection to low copy numbers minimizes the potential for primer-dimer formation More Information Brilliant II FAST SYBR® Green QPCR Reagents Accelerated cycling protocol that completes 40 cycles of PCR in approximately 48 minutes Rapid hot-start capability to minimize non-specific product formation while reducing run time Improved performance with earlier Ct detection across a broad dynamic range delivers increased sensitivity of detection of low copy number targets More Information

	Brilliant Multiplex QPCR Reagent Amplifies up to 4 targets in a single reaction Simultaneously amplifies low and high abundance targets Maximizes analysis of limited or rare samples Allows detection of multiple targets plus an internal control More Information QPCR Reference Total RNA, Human and Mouse High-quality total RNA control for quantitative PCR gene expression analysis An optimized blend of RNA for either human or mouse, derived from cell lines representing different tissue Greater consistency than RNA references derived from tissue sources Maximum representation of low, medium and high abundant gene transcripts Manufactured at industrial scale lot sizes and stringently quality-controlled for minimized lot-to-lot variation No detectable levels of genomic DNA More Information
	Alien QRT-PCR Inhibitor Alert High-quality external control for detecting inhibitors in RNA samples for QRT-PCR experiments High sensitivity to various inhibitors No significant homology to any known sequence Ideally suited for assay standardization applications More Information

	SAMPLE PURIFICATION Agilent offers a complete range of kits for fast and effective isolation and extraction of nucleic acids. DNA Purification Nucleic Acid Extraction RNA Purification

	Industry Leading Support for Our Customers We understand that the key to your success is more than just great products; you also need reliable technical support to help you solve whatever challenges are in front of you. That’s why we make our staff easily accessible to you by phone or online. More than just technicians, our support team consists of scientists and application experts with deep experience in qPCR technology. So when you call or contact us with an issue, you get the full attention of a qPCR expert who is trained and dedicated to solving your problem, and getting you back to work. Click here to find your representative or to contact Agilent Technologies Technical Services.


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