BestKeeper^© Software

• Original Publication in Biotechnology Letters 26:  509-515  (2004)
• Download BestKeeper software version 1
• Computer configurations 
• Description of  BestKeeper  software
  

The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either upor down-regulation under some experimental conditions. The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index. The index can be compared with further ten target genes to decide, whether they are differentially expressed under an applied treatment. All data processing is based on crossing points. The BestKeeper software tool was validated on four housekeeping genes and 10 members of the somatotropic axis differentially expressed in bovine corpora lutea total RNA. The BestKeeper application and necessary information about data processing and handling can be downloaded on here.

=>  download  BestKeeper version 1  <=
BestKeeper-1.zip

download  WinZip 8.1 

The BestKeeper Excel spreadsheet ZIP files is password protected.
To get the password by automatic response please contact genequan@wzw.tum.de
or  contact    password@gene-quantification.info

By receiving your requested passwords, we will include your e-mail address to our Gene-Qunatification.info and real-time-PCR.info mailing list, and you will receive our monthly appearing Gene Quantification Newsletter called qPCR NEWS and new information about real-time PCR hardware, software and chemistries.

Important note - Computer configurations

In order to be able to run the Relative Expression Software Tool correctly 
in all Microsoft Excel versions and to maintain the macro-virus protection 
for unknown documents as well active, it is recommended to activate 
the 'Trust all installed add-ins and templates' checkbox under

Tools => Macro => Security => Trusted Sources
or
Tools => Macro => Security => Security Level

and to set the 'Security Level' to 'Medium'

as shown in the following two screen shot pictures.

Michael W. Pfaffl (2003) Determination of most stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper© - Excel© spreadsheet tool using a Repeated Pair-wise Correlation and Regression Analysis 21. Dechema Jahrestagung der Biotechnologen 2.-4. April 2003