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qPCR EfficiencyChips / Lab on chipproblems derived from housekeeping genesdetermination of the best suited housekeeping genes and comparision with target genesdetermination of the best suited housekeeping genesAssumption-free analysis of quantitative real-time PCR dataverification of array results by real-time RT-PCRRNAinterferencereal-time RT-PCRcalculation of real-time PCR efficiency (E)real-time RT-PCR quantification strategiesabsolute quantification using real-time PCRrelative quantification using real-time PCR
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Technical University of MunichCenter of Life Sciences WeihenstephanInstitute of Physiology