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qPCR Efficiency qPCR Symposium 2010

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mRNA structure and analysis methodsclassical block RT-PCRcompetitive RT-PCRreal-time RT-PCRcalculation of real-time PCR efficiency (E)available real-time PCR platformsfluorescence detection chemistryoptimization strategies for kinetic RT-PCRreal-time RT-PCR quantification strategiesabsolute quantification using real-time PCRrelative quantification using real-time PCR

Relative Expression Software Tool (C)download of software tools

problems derived from housekeeping genes
determination of the best suited housekeeping genes and comparision with target genes


Technical University of Munich
Center of Life Sciences Weihenstephan
Institute of Physiology