We provide the
presentations via movie streaming technology in high quality - high resolution and perfect sound quality
in high speed - on any internet browser or mobile device,
including Android or iOS.
Our highly appreciated courses are suitable for
technicians, post-graduate students or anyone that wants to learn more
about real-time PCR and real-time PCR related technologies. Most of our
courses have both theoretical and practical parts where the
get to do experiments themselves under experienced supervision.
Different course modules are available at different occasion and you
may take a full week course or just a single course module
course event calendar
2-day qPCR course
25th-26th June 2014, Office Center Munich, Germany
Entire qPCR workflow covered in two
days . Focus on gene
expression (including microRNA) . Theoretical background and practical
data-analysis.Complemented with training on Biogazelle’s qbase+
software . Focus on experiment design and data-analysis . Attention for
validation and quality control
FIVE GOOD REASONS TO ATTEND:
Throughout the course the trainers explain how quality
control at each step in the qPCR workflow ensures accurate results.
reliable qPCR study begins with good experiment
design. On the first day of the course the principles of experiment
complemented with practical exercises.
Normalization using a non-validated housekeeping gene
may lead to errors more than 3-fold in 25% of the experiments. To avoid
errors, the students learn how to determine which and how many
Statistical analysis of qPCR results remains a
bottleneck for many researchers. The course covers descriptive
summarize qPCR results; key guidelines are provided to facilitate the
of appropriate statistical tests.
Biogazelle’s qPCR course allows the students to master
the MIQE guidelines and publish their qPCR results accordingly.
WHAT YOU GET:
Interactive training by qPCR
experts with proven track
record, a 2-month premium qbase+ license, a package of references and
notes on qPCR design and analysis. The course is catered (lunch and
TARGET AUDIENCE: The
course is intended for all qPCR
users with a basic level of experience.
750 EUR for
2 days. 20 places are available.
The workshop will focus on real-time PCR in gene expression
studies and will also cover other applications such as microorganism
detection, single cell qPCR, digital PCR. Workshop will include
extensive practical sessions and troubleshooting section.
workshop is organised in collaboration with National Institute of
Biology. The lectures and dry-lab hands-on sessions will take place at
theMarine Biological Station
(MBS)in Piran, situated directly
on the Mediterranean coast. The wet-lab sessions will take place in ISO
accredited laboratories ofNational Institute of
Biologyin Ljubljana which include
state-of-the-art qPCR centre.
sessions: Extensive practical sessions willl be located
in ISO 9001 and ISO 17025 accredited laboratories at the
state-of-the-art qPCR centre of National Institute of Biology in
Ljubljana (Transport will be organised and included in the workshop
will cover: review of qPCR theory, practical guidance on
sample preparation and RNA quality control, amplicon design, quality
assurance as well as hands-on lab and data analysis sessions.
Instructors, all experienced researchers and users of qPCR technology,
will also be available for discussions.
ADVANTAGES:Work in a small group,
individual approach, complete qPCR experiment covered - lab work,
quality assurance, data analysis etc., plenty of time for debates and
questioning, excellent lecturers and instructors, hands-on lab
work in ISO accredited environment, relaxed atmosphere at the seaside,
meeting other researchers from around the world, learning in teams etc.
Join us in September and
tame the knowledge of real-time PCR in a lovely, relaxing and energetic
environment of an old coastal town of Piran, a pearl of Slovenian
coast. The workshop is suitable for beginners as well as for advanced
profiling of RNA and protein using proximity ligation assay
Molecular tools such
as PCR and ELISA allow the sensitive and specific
detection of nucleic acids and proteins, respectively, and are widely
used as research tools as well as for the diagnosis of a broad range of
human, animal and plant diseases. The proximity ligation assay
is an innovative quantitative, sensitive and relatively inexpensive
immunoassay platform that combines the exquisite sensitivity and
dynamic range of real-time PCR with antibody-based detection of
proteins and other analytes. PLA offers several advantages over
traditional ELISAs, including better sensitivity (~100-fold) and
broader dynamic range (4 logs vs. 2.5 logs), simpler workflow
(requiring no wash steps) and faster time to results (<2.5 hours vs.
>5 hours). PLA is useful for the analysis of differential gene
expression, protein-protein interactions as well as post-translational
modifications and extends the limits of biomarker quantification.
Conventional methods for protein analysis are challenging to implement
into diagnostic assays because they are relatively insensitive, not
readily applied to quantification or rely on specialised equipment.
The EMBO workshop
will cover the background to PLA, introduce the
concept of quantitative protein detection and aims to inaugurate
MIQE-style guidelines for PLA. The presenters will be scientists who
have extensive experience of the technique. Examples of studies will be
presented to illustrate the use of PLA for simultaneous detection of
RNA and protein in diverse sample types, including single cells,
focusing on technical requirements for a functioning assay. The major
objective of the event is to provide sufficient technical expertise to
enable scientists, especially young researchers, to implement the
technique with ease into their workflow. An additional objective is to
provide the opportunity for those working with PLA to share experiences
and gain a deeper understanding of the method by interacting with the
leaders in the field.
Topics to be covered
Simultaneous quantification of RNA and protein
PLA Protein quantification: from assay design
to data analysis
Duolink® in situ PLA
Multi-analyte analysis in single tumour cells
PLA for the early and specific detection of
MIQE guidelines for PLA
Young researchers’ project presentations
Duolink, Olink, and PLA are registered
trademarks of Olink AB
The EMBO Workshop: Unravelling Biological
Secrets by Single-Cell
Expression Profiling will take place from 25-26 September. For further
information look below.
Why You Should Apply
The analysis of transcripts in minute samples is often used to increase
the knowledge in a plethora of research fields. Precise sampling is
crucial for the success of the approach and depends on the nature of
the sample and the goal of the experiment. This course will present
flow cytometry and laser capture microdissection as methods to obtain
single cells for the subsequent gene expression analysis, which cannot
be performed by conventional RNA methods due to their low sensitivity.
This course will present 3 different techniques to assess gene
expression: qRTPCR, Next Generation Sequencing and the Fluidigm system.
Data collected by these methods will be analysed in the very crucial
session of data analysis, which constitutes another strong component of
This course targets scientists who are entering the field of single
cell analysis and want to learn about different preparation methods and
gene expression profiling methods. The participants will learn about
the complete workflow and gain understanding about the possibilities
and limitations of the approach. The course will cover lectures and
hands-on sessions by experts in the fields and is aimed for PhD
students, postdoctoral fellows or other scientists with a solid qPCR
and molecular biology background.
Biological Secrets by Single-Cell Expression Profiling
Registration is now open for this event. Please
The EMBO Practical Course: Single-Cell Gene
Expression Analysis will take place a week before the workshop. If you
are interested in this course please look above.
Recent studies have demonstrated that the top-down approach of studying
a group of cells of the same type in order to infer what is happening
in an individual cell is wrong. The differences among cells of the same
population are often dramatic and so can be the consequences arising
from it. Recently, new technological advances make it possible to have
high resolution genomic and transcriptomic investigations at the
single-cell level. This has led to the implementation of single-cell
methods across a plethora of research fields.
This workshop aims at presenting these breakthrough advances to
scientists from different fields and creating a focused platform where
they can listen, present and discuss relevant biological findings that
have been made or can be achieved through these approaches.
The workshop will be mainly divided in two sessions
Due to the duration of the meeting the organisers decided to not
include more than 2 sessions in order to leave enough time for informal
discussions and networking opportunities. The organisers, who have
attended conferences in this field, want to specifically promote a
smaller, interactive meeting mainly dedicated to promote networking and
collaboration opportunities for early stage researchers.
This course is organised in collaboration with Dr. J.T. den Dunnen,
Leiden Genome Technology Center, Human Genetics, LUMC, Leiden
Aim of the course:
DNA sequencing technologies are currently developing at an incredible
speed and new applications are presented monthly, incl. DNA/RNA
profiling, ChIP-seq, re-sequencing, de novo genome sequencing and
metagenomics. The aim of this course is to introduce next generation
sequencing technologies, highlight different applications of these new
technologies, and show ways to analyse data.
This course has been developed for technicians and PhD students who are
interested in, planning, or already working with next-generation
sequencing. We welcome researchers from both the genomics and
bioinformatics fields. Currently available technologies, applications,
as well as hardware and software solutions will be presented and
We offer a three-day short-course consisting of a series of lectures on
the technologies and applications of next generation sequencing and
"hands-on" data analysis practicals. Furthermore we offer an additional
course day for those partcipants who would like to get more skills in
Linux based GAPSS data analysis pipelines. All participants will
receive a practical manual containing all used protocols.
qPCR Symposia, Meetings, and
& NGS 2015 Event -- Advanced Molecular Diagnostics for Biomarker
7th international qPCR & NGS Event -- Symposium &
Industrial Exhibition & Application Workshops
23-27 March 2015, in Freising-Weihenstephan, Technical University of
Munich, Weihenstephan, Germany
Central topic: Advanced
Molecular Diagnostics for Biomarker Discovery
Talk & Poster Sessions:
topic: Advanced Molecular
Diagnostics • Main
topic: Biomarker Discovery • Main
topic: Next Generation Sequencing
- NGS overview talks - information technology in the
era of NGS
- Pre NGS - sample prep & setup & library
- NGS – new sequencing technologies (e.g. single
molecule and pore sequencing)
- NGS – data analysis (data management, mapping,
alignment algorithms, data de novo assembly )
- NGS – diagnostic applications
• MicroGenomics & Single-cells diagnostics
• Circulating Nucleic Acids
• Molecular Diagnostics in Agriculture, Veterinary
Medicine & Environmental Science
• MIQE & QM & Standardisation strategies in
• Digital PCR & Nano-fluidics
• Non-coding RNAs -- microRNA, small RNAs, long
• qPCR Data Analysis -- BioStatistics &
Bustin, Mikael Kubista, Vladimir Benes, Jim Huggett, Jo Vandesompele,
Michael W. Pfaffl
This congress will be the first of its kind in France and Europe.
This will be a two day event welcoming 250 scientists from all over the
world. Targeted participants are international researchers working in
all areas covered by microgenomics.
Today, one of the biggest challenges for biologists is the ability to
understand and analyze the genome expressed in a given cell type in the
tissue environment, which means you can specifically isolate these
cells and work on very small quantities of biological material.
The aim of the congress is to bring together internationally renowned
experts in the field and offer an overview of the present knowledge for
obtaining high quality molecules and future developments in "omic"
tools (DNA, RNA and protein) to genome analysis and its expression at
the cell level.
The latest advances in technology in the field will be introduced (flow
cytometry, laser capture microdissection, DEParray, NGS, digital PCR,
WGA, LC-MS/MS, RPLA, etc).
The symposium will be divided into four sessions introduced by keynote
lecturers, experts in their field :
1- Sampling methods (flow cytometry, laser capture microdissection, DEP
2- Microgrenomics and DNA
3- Microgenomics and RNA and small RNA
4- Microgrenomics and Proteins
is pleased to present the 5th Advances in qPCR conference. In line with
evolving field, special focus will also be given to dPCR at this event.
New developments in qPCR technology will be showcased, concentrating on
improvements to qPCR design, the acquisition of accurate data and
data analysis. Focus will also be given to the use of dPCR in the
covering applications such as infectious disease diagnostics and cancer
detection. Attending this event will provide you with many
network with peers, helping you to build new relationships and optimise
the Organisation Committee and the Scientific Board it is
a great pleasure to invite you to the 6th International qPCR
& NGS 2013 Event. The event is divided in a 3-day scientific Symposium with an Industrial
and various 2-day Application
the Center of
Life Science in Freising Weihenstephan,
Technische Universität München (Germany).
in the previous meetings [ qPCR
2005 in LeipzigqPCR
2010 in Vienna and qPCR 2011 ] led us
to the decision to repeat the
spring 2013. We expect 500-600
coming from all over the world,
in 2011 we could welcome participants from 56 contries, and roughly 30-40
international companies in
the qPCR Industrial Exhibition.
set the date for the qPCR & NGS
- 22nd March 2013. The event location is the central lecture hall
and the foyer at TUM (Technical University of Munich) in Freising
Weihenstephan, Germany (download Google Maps link or Google
The TUM and the Biotech region around Munich is
part of the largest Biotech cluster in Europe, located close to the
Munich airport in the heart of Bavaria.
The focus of the qPCR 2013 Event will be on:
Thinking in Molecular Diagnostics
academic researchers and
industrial contributors in the field will participate in the
symposium, which will be an arena for fruitful discussions between
researchers of different backgrounds. The Symposium Talks, Poster
Exhibition and associated qPCR & NGS
Workshops offer an
the present knowledge and future developments in qPCR, next generation
sequencing and gene
expression measurement technology and
its wide applications in research.
focus on 74
lectures and a huge
poster exhibition (105 posters)will
recognised experts in their field. The emphasis will be
on unbiased, didactic
reknown speakers will be participating in a lively and exciting
programme enabling the valuable exchange of information in the qPCR
presented by selected invited speakers,
third will be selected from the submitted
abstracts and one
third will be presented by qPCR related company
scientific contributions will be published
in the qPCR
Proceedings (ISBN to be announced).
selected invited academic and industrial speakersand
application notes fromindustrial
be published in aMETHODS special
by Michael W. Pfaffl(published
the meeting all participants will receive a print copy of this special
Methods issue "Transcriptional
Biomarkers" METHODS 2013 59(1) pages 1-192
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
will cover areas such as:
QPCR assay design and
Template handling and
Focus on the
optimisation of RT-qPCR
Various approaches to
Working with limiting
option for challenging assays
LNA technology and
Level 1 - terrified
Level 2 - something
for everyone but a background knowledge is assumed
Level 3 - improvers,
seminars focused on specific areas of interest
Level 4 - brave and
experienced, delving into the fine detail and the more troublesome
aspects of the technique