Our highly appreciated courses are suitable for
technicians, post-graduate students or anyone that wants to learn more
about real-time PCR and real-time PCR related technologies. Most of our
courses have both theoretical and practical parts where the
get to do experiments themselves under experienced supervision.
Different course modules are available at different occasion and you
may take a full week course or just a single course module
course event calendar
2-day qPCR course
25th-26th June 2014, Office Center Munich, Germany
Entire qPCR workflow covered in two
days . Focus on gene
expression (including microRNA) . Theoretical background and practical
data-analysis.Complemented with training on Biogazelle’s qbase+
software . Focus on experiment design and data-analysis . Attention for
validation and quality control
FIVE GOOD REASONS TO ATTEND:
Throughout the course the trainers explain how quality
control at each step in the qPCR workflow ensures accurate results.
reliable qPCR study begins with good experiment
design. On the first day of the course the principles of experiment
complemented with practical exercises.
Normalization using a non-validated housekeeping gene
may lead to errors more than 3-fold in 25% of the experiments. To avoid
errors, the students learn how to determine which and how many
Statistical analysis of qPCR results remains a
bottleneck for many researchers. The course covers descriptive
summarize qPCR results; key guidelines are provided to facilitate the
of appropriate statistical tests.
Biogazelle’s qPCR course allows the students to master
the MIQE guidelines and publish their qPCR results accordingly.
WHAT YOU GET:
Interactive training by qPCR
experts with proven track
record, a 2-month premium qbase+ license, a package of references and
notes on qPCR design and analysis. The course is catered (lunch and
TARGET AUDIENCE: The
course is intended for all qPCR
users with a basic level of experience.
750 EUR for
2 days. 20 places are available.
INFORMATION AND ENROLLMENT:
information of qbase+ software and Biogazelle: www.biogazelle.com
The workshop will focus on real-time PCR in gene expression
studies and will also cover other applications such as microorganism
detection, single cell qPCR, digital PCR. Workshop will include
extensive practical sessions and troubleshooting section.
workshop is organised in collaboration with National Institute of
Biology. The lectures and dry-lab hands-on sessions will take place at
theMarine Biological Station
(MBS) in Piran, situated directly
on the Mediterranean coast. The wet-lab sessions will take place in ISO
accredited laboratories of National Institute of
Biology in Ljubljana which include
state-of-the-art qPCR centre.
: Extensive practical sessions willl be located
in ISO 9001 and ISO 17025 accredited laboratories at the
state-of-the-art qPCR centre of National Institute of Biology in
Ljubljana (Transport will be organised and included in the workshop
review of qPCR theory, practical guidance on
sample preparation and RNA quality control, amplicon design, quality
assurance as well as hands-on lab and data analysis sessions.
Instructors, all experienced researchers and users of qPCR technology,
will also be available for discussions.
ADVANTAGES: Work in a small group,
individual approach, complete qPCR experiment covered - lab work,
quality assurance, data analysis etc., plenty of time for debates and
questioning, excellent lecturers and instructors, hands-on lab
work in ISO accredited environment, relaxed atmosphere at the seaside,
meeting other researchers from around the world, learning in teams etc.
Join us in September and
tame the knowledge of real-time PCR in a lovely, relaxing and energetic
environment of an old coastal town of Piran, a pearl of Slovenian
coast. The workshop is suitable for beginners as well as for advanced
profiling of RNA and protein using proximity ligation assay
7 July 2014 | Chelmsford, United Kingdom
|ABOUT THE WORKSHOP
||Molecular tools such
as PCR and ELISA allow the sensitive and specific
detection of nucleic acids and proteins, respectively, and are widely
used as research tools as well as for the diagnosis of a broad range of
human, animal and plant diseases. The proximity ligation assay
is an innovative quantitative, sensitive and relatively inexpensive
immunoassay platform that combines the exquisite sensitivity and
dynamic range of real-time PCR with antibody-based detection of
proteins and other analytes. PLA offers several advantages over
traditional ELISAs, including better sensitivity (~100-fold) and
broader dynamic range (4 logs vs. 2.5 logs), simpler workflow
(requiring no wash steps) and faster time to results (<2.5 hours vs.
>5 hours). PLA is useful for the analysis of differential gene
expression, protein-protein interactions as well as post-translational
modifications and extends the limits of biomarker quantification.
Conventional methods for protein analysis are challenging to implement
into diagnostic assays because they are relatively insensitive, not
readily applied to quantification or rely on specialised equipment.
|The EMBO workshop
will cover the background to PLA, introduce the
concept of quantitative protein detection and aims to inaugurate
MIQE-style guidelines for PLA. The presenters will be scientists who
have extensive experience of the technique. Examples of studies will be
presented to illustrate the use of PLA for simultaneous detection of
RNA and protein in diverse sample types, including single cells,
focusing on technical requirements for a functioning assay. The major
objective of the event is to provide sufficient technical expertise to
enable scientists, especially young researchers, to implement the
technique with ease into their workflow. An additional objective is to
provide the opportunity for those working with PLA to share experiences
and gain a deeper understanding of the method by interacting with the
leaders in the field.
|Topics to be covered
- Simultaneous quantification of RNA and protein
- PLA Protein quantification: from assay design
to data analysis
- Duolink® in situ PLA
- Multi-analyte analysis in single tumour cells
- PLA for the early and specific detection of
- Digital PLA
- MIQE guidelines for PLA
- Young researchers’ project presentations
- Duolink, Olink, and PLA are registered
trademarks of Olink AB
||EMBO Practical Course
EMBL Heidelberg, Germany Friday 19 September - Thursday 25 September
2014 Application deadline: Thursday 26 June 2014
Abstract deadline: Thursday 26 June 2014
Why You Should Apply
- Registration is now open, please register here.
- The EMBO Workshop: Unravelling Biological
Secrets by Single-Cell
Expression Profiling will take place from 25-26 September. For further
information look below.
The analysis of transcripts in minute samples is often used to increase
the knowledge in a plethora of research fields. Precise sampling is
crucial for the success of the approach and depends on the nature of
the sample and the goal of the experiment. This course will present
flow cytometry and laser capture microdissection as methods to obtain
single cells for the subsequent gene expression analysis, which cannot
be performed by conventional RNA methods due to their low sensitivity.
This course will present 3 different techniques to assess gene
expression: qRTPCR, Next Generation Sequencing and the Fluidigm system.
Data collected by these methods will be analysed in the very crucial
session of data analysis, which constitutes another strong component of
This course targets scientists who are entering the field of single
cell analysis and want to learn about different preparation methods and
gene expression profiling methods. The participants will learn about
the complete workflow and gain understanding about the possibilities
and limitations of the approach. The course will cover lectures and
hands-on sessions by experts in the fields and is aimed for PhD
students, postdoctoral fellows or other scientists with a solid qPCR
and molecular biology background.
Biological Secrets by Single-Cell Expression Profiling
EMBL Heidelberg, Germany Thursday 25 September - Friday 26 September
- Registration is now open for this event. Please
- The EMBO Practical Course: Single-Cell Gene
Expression Analysis will take place a week before the workshop. If you
are interested in this course please look above.
Recent studies have demonstrated that the top-down approach of studying
a group of cells of the same type in order to infer what is happening
in an individual cell is wrong. The differences among cells of the same
population are often dramatic and so can be the consequences arising
from it. Recently, new technological advances make it possible to have
high resolution genomic and transcriptomic investigations at the
single-cell level. This has led to the implementation of single-cell
methods across a plethora of research fields.
This workshop aims at presenting these breakthrough advances to
scientists from different fields and creating a focused platform where
they can listen, present and discuss relevant biological findings that
have been made or can be achieved through these approaches.
The workshop will be mainly divided in two sessions
1. Novel technologies
2. Unravelling biological questions
Due to the duration of the meeting the organisers decided to not
include more than 2 sessions in order to leave enough time for informal
discussions and networking opportunities. The organisers, who have
attended conferences in this field, want to specifically promote a
smaller, interactive meeting mainly dedicated to promote networking and
collaboration opportunities for early stage researchers.
Introduction to Next
Generation Sequencing; technologies, applications, and data analysis
Course Date: April 01-04 2014, Breda (NL)
This course is organised in collaboration with Dr. J.T. den Dunnen,
Leiden Genome Technology Center, Human Genetics, LUMC, Leiden
Aim of the course:
DNA sequencing technologies are currently developing at an incredible
speed and new applications are presented monthly, incl. DNA/RNA
profiling, ChIP-seq, re-sequencing, de novo genome sequencing and
metagenomics. The aim of this course is to introduce next generation
sequencing technologies, highlight different applications of these new
technologies, and show ways to analyse data.
This course has been developed for technicians and PhD students who are
interested in, planning, or already working with next-generation
sequencing. We welcome researchers from both the genomics and
bioinformatics fields. Currently available technologies, applications,
as well as hardware and software solutions will be presented and
We offer a three-day short-course consisting of a series of lectures on
the technologies and applications of next generation sequencing and
"hands-on" data analysis practicals. Furthermore we offer an additional
course day for those partcipants who would like to get more skills in
Linux based GAPSS data analysis pipelines. All participants will
receive a practical manual containing all used protocols.
qPCR Symposia, Meetings, and
The first Symposium on Microgenomics in
France and Europe
15-16 May 2014
Pierre and Marie Curie University, "Les Cordeliers" Campus,
15 rue de l'Ecole de Medicine, Paris 75006, France
This congress will be the first of its kind in France and Europe.
This will be a two day event welcoming 250 scientists from all over the
world. Targeted participants are international researchers working in
all areas covered by microgenomics.
Today, one of the biggest challenges for biologists is the ability to
understand and analyze the genome expressed in a given cell type in the
tissue environment, which means you can specifically isolate these
cells and work on very small quantities of biological material.
The aim of the congress is to bring together internationally renowned
experts in the field and offer an overview of the present knowledge for
obtaining high quality molecules and future developments in "omic"
tools (DNA, RNA and protein) to genome analysis and its expression at
the cell level.
The latest advances in technology in the field will be introduced (flow
cytometry, laser capture microdissection, DEParray, NGS, digital PCR,
WGA, LC-MS/MS, RPLA, etc).
The symposium will be divided into four sessions introduced by keynote
lecturers, experts in their field :
1- Sampling methods (flow cytometry, laser capture microdissection, DEP
2- Microgrenomics and DNA
3- Microgenomics and RNA and small RNA
4- Microgrenomics and Proteins
Advances in qPCR & dPCR, 14-15 May, Barcelona, Spain
is pleased to present the 5th Advances in qPCR conference. In line with
evolving field, special focus will also be given to dPCR at this event.
New developments in qPCR technology will be showcased, concentrating on
improvements to qPCR design, the acquisition of accurate data and
data analysis. Focus will also be given to the use of dPCR in the
covering applications such as infectious disease diagnostics and cancer
detection. Attending this event will provide you with many
network with peers, helping you to build new relationships and optimise
Clinical Applications of qPCR & dPCR
- New Developments in Digital PCR
Single Cell Analysis
Standardisation & Validation
Professor/Founder, TATAA Biocenter
Professor, University of Utah
Running alongside the Advances in qPCR & dPCR conference will be
exhibition, covering the latest
technological advances and associated services from leading solution
within this field. Registered delegates will also have access to the
co-located conference tracks, ensuring a cost effective trip: Advances
in NGS & Big Data, High
Content Analysis, Advances
in Automation & Robotics, Advances
in Cellular Assays & Cell Culture.
Submission deadline – 2nd May 2014
Early Bird /
2for1 Offer – Register by 2 April 2014
the Organisation Committee and the Scientific Board it is
a great pleasure to invite you to the 6th International qPCR
& NGS 2013 Event. The event is divided in a 3-day scientific Symposium with an Industrial
and various 2-day Application
the Center of
Life Science in Freising Weihenstephan,
Technische Universität München (Germany).
in the previous meetings [ qPCR
2004 qPCR 2005 qPCR
2005 in Leipzig qPCR
2007 qPCR 2009
2010 in Vienna and qPCR 2011 ] led us
to the decision to repeat the
spring 2013. We expect 500-600
coming from all over the world,
in 2011 we could welcome participants from 56 contries, and roughly 30-40
international companies in
the qPCR Industrial Exhibition.
set the date for the qPCR & NGS
- 22nd March 2013. The event location is the central lecture hall
and the foyer at TUM (Technical University of Munich) in Freising
Weihenstephan, Germany (download Google Maps link or Google
The TUM and the Biotech region around Munich is
part of the largest Biotech cluster in Europe, located close to the
Munich airport in the heart of Bavaria.
The focus of the qPCR 2013 Event will be on:
Thinking in Molecular Diagnostics
academic researchers and
industrial contributors in the field will participate in the
symposium, which will be an arena for fruitful discussions between
researchers of different backgrounds. The Symposium Talks, Poster
Exhibition and associated qPCR & NGS
Workshops offer an
the present knowledge and future developments in qPCR, next generation
sequencing and gene
expression measurement technology and
its wide applications in research.
focus on 74
lectures and a huge
poster exhibition (105 posters) will
recognised experts in their field. The emphasis will be
on unbiased, didactic
information exchange. Internationally
reknown speakers will be participating in a lively and exciting
programme enabling the valuable exchange of information in the qPCR
presented by selected invited speakers,
third will be selected from the submitted
abstracts and one
third will be presented by qPCR related company
scientific contributions will be published
in the qPCR
Proceedings (ISBN to be announced).
selected invited academic and industrial speakers and
application notes from industrial
be published in a METHODS special
by Michael W. Pfaffl (published
January 2013). At
the meeting all participants will receive a print copy of this special
Methods issue "Transcriptional
Biomarkers" METHODS 2013 59(1) pages 1-192
Agenda is available - 74 talks and 105 posters accepted !
version of the agenda
of the Agenda Summary of the Poster
and Poster sessions:
- Main Topic: Next
Generation Sequencing (NGS)
- Main Topic: Transcriptional
- Single-cells diagnostics
- MIQE & QM
strategies in qPCR
- non-coding RNAs -
and long non-coding RNAs
- Digital PCR
- Biogazelle - qBASEplus - qPCR data analysis
- MultiD - GenEx - qPCR data analysis seminar
- Life TEchnologies - digital PCR seminar
- Genomatix - NGS data analysis seminar
The forum „Trends in
Microanalytical Systems and Chip-based Bioanalysis“ will be organized
by Lausitz University of Applied Sciences, BioResponse e. V. together
with the Center for Molecular Diagnostics and Bioanalysis (ZMDB) as the
7th “Senftenberger Innovationsforum Multiparameteranalytik”. The
scope of the workshop comprises microanalytical systems and chip-based
bioanalytics with a focus on the real-time monitoring of amplification
reactions and related multiplex technologies. We encourage also a
submission of abstracts on new analytical technologies, applications,
and instrumentation, instrumental cell based bioanalytics, use of
micro/nano-structured materials, and micro- and nanoparticles and
The workshop will include oral lectures, poster
presentations, an industrial exhibition (Alere Technologies GmbH,
Analytik Jena AG, Attomol GmbH, IBA GmbH, Medipan GmbH, PolyAn GmbH,
Scienion AG) and demonstration of prototypes of analytical devices
(Mainz Institute for Microtechnology, Fraunhofer Institute for
Biomedical Engineering, Lausitz University of Applied Sciences).
The format of the conference intends active participation and
interaction of experts from research institutions and industry.
The participation is free of charge. Prior registration is requested
Call for Papers: Selected papers will be published in a special issue
of Microchimica Acta. The selection will be performed by peer review
procedure. Enquires concerning paper submission:
email@example.com. Deadline for abstract submission: March
Call for Posters/Call for Presentations: You are kindly invited to
present results of your research or new products. We accept abstracts
for talks and posters. A prize for the best posters will be awarded.
Deadline for abstract submission: March 1st, 2013. Please contact
Christine Mißler (firstname.lastname@example.org).
• Agata Jósefiak - Centrum
Badan DNA Sp.z.o.o. Poznan (Poland)
• Andrew deMello - ETH Zürich
• Dirk Kuhlmeier -
Fraunhofer-Institut für Zelltherapie und Immunologie IZI (Germany)
• Dirk Laßner - Institut
Kardiale Diagnostik und Therapie GmbH Berlin (Germany)
• Frank T. Hufert -
Universität Göttingen (Germany)
• Ingo Klimant - Technische
Universität Graz (Austria)
• Karina Weber - Institut für
Photonische Technologien (IPHT) &
Friedrich-Schiller-Universität Jena (Germany)
• Michael W. Pfaffl - Technische
Universität München (TUM) (Germany)
• Oliver Strohmeier - (HSG-IMIT)
• Ralf Ehricht - Alere
Technologies GmbH (Germany)
• Ralf Himmelreich - IMM Institut
für Mikrotechnik Mainz GmbH Mainz (Germany)
• Sebastian Kersting -
Fraunhofer-Institut für Biomedizinische Technik IBMT (Germany)
• Simon Wadle - (HSG-IMIT)
• Ute Resch-Genger - BAM
Bundesanstalt für Materialforschung und -prüfung (BAM)
• Werner Lehmann - Attomol GmbH
Genomics Research Europe
conference and exhibition
the completion of the human genome in 2003 there have been constant
challenges to genomic research technologies and public health
strategies. Persistent challenges faced by those in this discipline
will be addressed at the meeting. This conference aims to provide
delegates with an improved awareness of current developments in the
following fields: New Applications in PCR & Next-Generation
Sequencing, and Epigenetics, microRNAs and Non-coding RNAs in Disease.
delegates will have access to both meetings ensuring a cost-effective
trip. As well as an array of international speakers and poster
presentations, our exhibitors will display a repertoire of cutting edge
research tools and delegates will have the opportunity to establish and
renew global collaborations.
Seminars and Education Programs:
of charge web
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
will cover areas such as:
- QPCR assay design and
- Template handling and
- Focus on the
optimisation of RT-qPCR
- Various approaches to
- Working with limiting
- Data analysis
- Assay improvement
option for challenging assays
- LNA technology and
- Level 1 - terrified
- Level 2 - something
for everyone but a background knowledge is assumed
- Level 3 - improvers,
seminars focused on specific areas of interest
- Level 4 - brave and
experienced, delving into the fine detail and the more troublesome
aspects of the technique
|Mudassir Mohammed, Ph.D.
||An Introduction to qPCR
|Valerie Hawkes, Ph.D.
||Assay Design and
|Neven Zoric, M.Sc.
||Approaches to Normal qPCR —
Facing the Challenge
|Tania Nolan, Ph.D.
||Deriving a Troubleshooting
|Mikael Kubista, Prof.
||A Statistical Approach to
qPCR Gene Expression
|Marina Tshuikina, Ph.D.
||An Introduction to
Epigenetics Analysis by qPCR
|Valerie Hawkes, Ph.D.
Discrimination and Sensitivity of
qPCR Assays using LNA
|Chris Bass, Ph.D.
||Development of DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito Disease Vector Populations
|Michael Pfaffl, Ph.D.
||Influence of RNA Integrity
on Real-Time RT-PCR
||Technical Tips on LCM
Extraction for mRNA
||cDNA Amplification with
Whole Genome Amplification Kit
|Tanya Novak, M.Sc.
||The SPUD Assay Has an
Important Role in the
Interpretation of Detecting Pneumocystis DNA in Clinical Specimens