real-time qPCR workshops
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Upcoming events:
  previous qPCR events:
qPCR 2004   qPCR 2005   qPCR 2005 in Leipzig   qPCR 2007    qPCR 2009    qPCR 2010 in Vienna  qPCR 2007 in US   qPCR 2008 in US qPCR 2009 in US  qPCR 2010 in Gothenburg  qPCR 2010 in US   qPCR 2011  qPCR 2011 in Prague   qPCR & NGS 2013

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qPCR & NGS 2015


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Amplify your knowledge!

280 talks recorded at
qPCR 2010 &
qPCR 2011
qPCR & NGS 2013 & 2015


We provide the presentations via movie streaming technology in high quality - high resolution and perfect sound quality in high speed - on any internet browser or mobile device, including Android or iOS.

qPCR Workshops:

qPCR curses are held regularly in Göteborg Sweden and Prague Czech Republic

Our highly appreciated courses are suitable for researchers, technicians, post-graduate students or anyone that wants to learn more about real-time PCR and real-time PCR related technologies. Most of our courses have both theoretical and practical parts where the participants get to do experiments themselves under experienced supervision. Different course modules are available at different occasion and you may take a full week course or just a single course module  =>  qPCR course event calendar

Course modules:

3 days Hands-on qPCR 2 days Hands-on qPCR  1 day Sample preparation

3 days Exp design & data analysis 2 days Exp design & data analysis  1 day Immuno-qPCR

2 days Single cell analysis
 1 day Multiplex PCR

 1 day HRM

 1 day MicroRNA analysis

 1 day Quality control in MDx

2-day qPCR courses


Entire qPCR workflow covered in two days . Focus on gene expression (including microRNA) . Theoretical background and practical hands-on data-analysis.Complemented with training on Biogazelle’s qbase+ software . Focus on experiment design and data-analysis . Attention for validation and quality control


1- Throughout the course the trainers explain how quality control at each step in the qPCR workflow ensures accurate results.

2- A reliable qPCR study begins with good experiment design. On the first day of the course the principles of experiment design are complemented with practical exercises.

3- Normalization using a non-validated housekeeping gene may lead to errors more than 3-fold in 25% of the experiments. To avoid such errors, the students learn how to determine which and how many reference genes to use.

4- Statistical analysis of qPCR results remains a bottleneck for many researchers. The course covers descriptive statistics to summarize qPCR results; key guidelines are provided to facilitate the selection of appropriate statistical tests.

5- Biogazelle’s qPCR course allows the students to master the MIQE guidelines and publish their qPCR results accordingly.


Interactive training by qPCR experts with proven track record, a 2-month premium qbase+ license, a package of references and course notes on qPCR design and analysis. The course is catered (lunch and pauses) on both days.

TARGET AUDIENCE:   The course is intended for all qPCR users with a basic level of experience.


More information of qbase+ software and Biogazelle:

Practical qPCR Workshop at Piran, the pearl of the Mediterranean coast!
Welcome to the qPCR Experience Boot Camp!
2nd - 5th September 2014, Piran, Slovenia

The workshop will focus on real-time PCR  in gene expression studies and will also cover other applications such as microorganism detection, single cell qPCR, digital PCR. Workshop will include extensive practical sessions and troubleshooting section.

The workshop is organised in collaboration with National Institute of Biology. The lectures and dry-lab hands-on sessions will take place at theMarine Biological Station (MBS) in Piran, situated directly on the Mediterranean coast. The wet-lab sessions will take place in ISO accredited laboratories of National Institute of Biology in Ljubljana which include state-of-the-art qPCR centre.

Workshop Summary 
Location: Piran, Slovenia
Duration: 4 days
Language: English

Practical sessions: Extensive practical sessions willl be located  in ISO 9001 and ISO 17025 accredited laboratories at the state-of-the-art qPCR centre of National Institute of Biology in Ljubljana (Transport will be organised and included in the workshop fee).

Workshop will cover: review of qPCR theory, practical guidance on sample preparation and RNA quality control, amplicon design, quality assurance as well as hands-on lab and data analysis sessions. Instructors, all experienced researchers and users of qPCR technology, will also be available for discussions. 

IMPORTANT ADVANTAGES: Work in a small group, individual approach, complete qPCR experiment covered - lab work, quality assurance, data analysis etc., plenty of time for debates and questioning, excellent lecturers and instructors,  hands-on lab work in ISO accredited environment, relaxed atmosphere at the seaside, meeting other researchers from around the world, learning in teams etc.
Join us in September and tame the knowledge of real-time PCR in a lovely, relaxing and energetic environment of an old coastal town of Piran, a pearl of Slovenian coast. The workshop is suitable for beginners as well as for advanced qPCR users.

EMBL Advanced Course -- Exosome Purification, Identification and Translation

EMBL Heidelberg, Germany
Wednesday 26 November - Friday 28 November 2014

A rapidly increasing number of researchers aim to purify and identify exosomes and many methodologies to isolate and analyse these vesicles are available and have been implemented.


A rapidly increasing number of researchers aim to purify and identify exosomes and many methodologies to isolate and analyse these vesicles are available and have been implemented. In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of exosomes (with practical sessions included)

  • Exosome purification (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques)
  • Exosome Characterization (Immunoblot and electron microscopy, Quantitative and qualitative analysis by flow cytometry, Structural and mechanical characterization by atomic force microscopy, Nanoparticle Tracking Analysis)
  • Exosome Composition (Lipidomics, Proteomics, Transcriptomics)
  • Exosome Biogenesis and Function (Molecular players for exosome biogenesis, Exosomes as diagnostics and/or therapeutics in pathophysiology)

Simultaneous profiling of RNA and protein using proximity ligation assay

7 July 2014 | Chelmsford, United Kingdom

ABOUT THE WORKSHOP Molecular tools such as PCR and ELISA allow the sensitive and specific detection of nucleic acids and proteins, respectively, and are widely used as research tools as well as for the diagnosis of a broad range of human, animal and plant diseases. The proximity ligation assay (PLA®) is an innovative quantitative, sensitive and relatively inexpensive immunoassay platform that combines the exquisite sensitivity and dynamic range of real-time PCR with antibody-based detection of proteins and other analytes. PLA offers several advantages over traditional ELISAs, including better sensitivity (~100-fold) and broader dynamic range (4 logs vs. 2.5 logs), simpler workflow (requiring no wash steps) and faster time to results (<2.5 hours vs. >5 hours). PLA is useful for the analysis of differential gene expression, protein-protein interactions as well as post-translational modifications and extends the limits of biomarker quantification.  Conventional methods for protein analysis are challenging to implement into diagnostic assays because they are relatively insensitive, not readily applied to quantification or rely on specialised equipment.

The EMBO workshop will cover the background to PLA, introduce the concept of quantitative protein detection and aims to inaugurate MIQE-style guidelines for PLA. The presenters will be scientists who have extensive experience of the technique. Examples of studies will be presented to illustrate the use of PLA for simultaneous detection of RNA and protein in diverse sample types, including single cells, focusing on technical requirements for a functioning assay. The major objective of the event is to provide sufficient technical expertise to enable scientists, especially young researchers, to implement the technique with ease into their workflow. An additional objective is to provide the opportunity for those working with PLA to share experiences and gain a deeper understanding of the method by interacting with the leaders in the field.

Topics to be covered include:
  • Simultaneous quantification of RNA and protein
  • PLA Protein quantification: from assay design to data analysis
  • Duolink® in situ PLA
  • Multi-analyte analysis in single tumour cells
  • PLA for the early and specific detection of pathogens
  • Digital PLA
  • MIQE guidelines for PLA
  • Young researchers’ project presentations
  • Duolink, Olink, and PLA are registered trademarks of Olink AB

EMBO Practical Course -- Single-Cell Gene Expression Analysis

EMBL Heidelberg, Germany Friday 19 September - Thursday 25 September 2014 Application deadline: Thursday 26 June 2014
Abstract deadline: Thursday 26 June 2014

Latest News
  • Registration is now open, please register here.
  • The EMBO Workshop: Unravelling Biological Secrets by Single-Cell Expression Profiling will take place from 25-26 September. For further information look below.
Why You Should Apply

The analysis of transcripts in minute samples is often used to increase the knowledge in a plethora of research fields. Precise sampling is crucial for the success of the approach and depends on the nature of the sample and the goal of the experiment. This course will present flow cytometry and laser capture microdissection as methods to obtain single cells for the subsequent gene expression analysis, which cannot be performed by conventional RNA methods due to their low sensitivity. This course will present 3 different techniques to assess gene expression: qRTPCR, Next Generation Sequencing and the Fluidigm system. Data collected by these methods will be analysed in the very crucial session of data analysis, which constitutes another strong component of the course.

This course targets scientists who are entering the field of single cell analysis and want to learn about different preparation methods and gene expression profiling methods. The participants will learn about the complete workflow and gain understanding about the possibilities and limitations of the approach. The course will cover lectures and hands-on sessions by experts in the fields and is aimed for PhD students, postdoctoral fellows or other scientists with a solid qPCR and molecular biology background.

EMBO Workshop -- Unravelling Biological Secrets by Single-Cell Expression Profiling

EMBL Heidelberg, Germany Thursday 25 September - Friday 26 September 2014

Latest News
  • Registration is now open for this event. Please register here.
  • The EMBO Practical Course: Single-Cell Gene Expression Analysis will take place a week before the workshop. If you are interested in this course please look above.

Recent studies have demonstrated that the top-down approach of studying a group of cells of the same type in order to infer what is happening in an individual cell is wrong. The differences among cells of the same population are often dramatic and so can be the consequences arising from it. Recently, new technological advances make it possible to have high resolution genomic and transcriptomic investigations at the single-cell level. This has led to the implementation of single-cell methods across a plethora of research fields.

This workshop aims at presenting these breakthrough advances to scientists from different fields and creating a focused platform where they can listen, present and discuss relevant biological findings that have been made or can be achieved through these approaches.

The workshop will be mainly divided in two sessions

   1. Novel technologies
   2. Unravelling biological questions

Due to the duration of the meeting the organisers decided to not include more than 2 sessions in order to leave enough time for informal discussions and networking opportunities. The organisers, who have attended conferences in this field, want to specifically promote a smaller, interactive meeting mainly dedicated to promote networking and collaboration opportunities for early stage researchers.

Principles, parameters and applications of PCR

Date: 7 April 2015
Location: Avans Hogeschool Breda, The Netherlands
Registration fee: €400,-

Real-time PCR in molecular diagnostics
Date: 8-10 April 2015
Location: Avans Hogeschool Breda, The Netherlands
Registration fee: €900,-


Introduction to Next Generation Sequencing; technologies and data analysis
Date: 14-17 April 2015
Location: Avans Hogeschool Breda, The Netherlands
Registration fee: € 1100,-


qPCR Symposia, Meetings, and Conferences:

20th World Congress on Advances in Oncology 
18th International Symposium on Molecular Medicine

October 8-10, 2015
Metropolitan Hotel ***** Athens, Greece

The continued interest and increasing support that our annual Congress on Advances in Oncology and Symposium of Molecular Medicine has gained over the years, motivates us to repeat this success this year in October 2015. Over the last 20 years we have consistently welcomed a broad international audience, which surpassed 500 participants in 2014.

The 20th World Congress on Advances in Oncology and the 18th International Symposium of Molecular Medicine will take place from 8-10 October, 2015.
The event will be held at the Metropolitan Hotel ***** Conference Center in Athens, Greece.

The aim of our conferences is to keep participants up to date with the latest scientific developments in Oncology and Molecular Medicine. Our conferences constitute a unique venue for basic and clinical research for the development of molecular diagnostics and cancer therapeutics. Some of the many topics that will be discussed are:

  • Biochemistry in Molecular Oncology
  • Immunology in Molecular Oncology
  • Experimental Carcinogenesis
  • Apoptosis/Hypoxia
  • Chemotherapy/Pharmacology
  • Tumor Microenvironment & Signaling
  • Cancer Inflammation & Differentiation
  • Cancer Therapeutics

  • Molecular Medicine
  • Molecular & Clinical Diagnostics
  • Quantitative PCR & Next Generation Sequencing
  • Discovery and Validation of Biomarkers
  • CTCs  &  Circulating Biomarkers
  • Regulative small RNAS in Cancer
  • … … and additional subjects relating to Oncology and Molecular Medicine.

In parallel to the scientific symposium of 2015, an Industrial Exhibition will take place, at which international companies, leaders in the fields of Oncology and Molecular Medicine, will present their latest technologies and services in clinical diagnostics. If you are interested in joining the exhibition, please contact us at

Further, two qPCR Workshops dedicated to ‘Molecular Diagnostics’, Biomarker Development’ and ‘qPCR Standardization’ in applying the MIQE guidelines, will take place before the symposium on 6-7 October (hosted by TATAA Biocenter Sweden).
  • Discovery and Validation of Expression Biomarkers - Experimental Design and Data Analysis
  • MIQE guidelines - How to get good quality control in qPCR
We look forward to your participation.

Yours sincerely,

Demetrios A. Spandidos

qPCR & NGS 2015 Event
7th international qPCR & NGS Event -- Symposium  &  Industrial Exhibition  &  Application Workshops
23-27 March 2015, in Freising-Weihenstephan, TUM School of Life Sciences
Technical University of Munich, Weihenstephan, Germany

Central topic:   Advanced Molecular Diagnostics for Biomarker Discovery

Symposium Talk & Poster Sessions:

•    Main topic:   Advanced Molecular Diagnostics
•    Main topic:   Biomarker Discovery
•    Main topic:   Next Generation Sequencing (NGS)
    - NGS overview talks - information technology in the era of NGS
    - Pre NGS - sample prep & setup & library generation
    - NGS – new sequencing technologies (e.g. single molecule and pore sequencing)
    - NGS – data analysis (data management, mapping, alignment algorithms, data de novo assembly )
    - NGS – diagnostic applications

•    MicroGenomics & Single-cells diagnostics
•    Circulating Nucleic Acids
•    Molecular Diagnostics in Agriculture, Veterinary Medicine, Food & Environmental Science
•    MIQE & QM & Standardisation strategies in molecular diagnostics
•    Digital PCR  &  Nano-fluidics
•    Non-coding RNAs -- microRNA, small RNAs, long non-coding RNAs
•    qPCR Data Analysis -- BioStatistics & BioInformatics

Scientific board:  Stephen Bustin, Mikael Kubista, Vladimir Benes, Jim Huggett, Jo Vandesompele, Michael W. Pfaffl

Regsiter for the symposium and the workshops =>
Download the event flyer =>
Event location on Google maps

qPCR & NGS 2015
Scientific coordination: Michael W. Pfaffl
Event organization: Sylvia Pfaffl bioMCC

Promotion Trailer qPCR & NGS 2015

Follow 200 talks from previous events
on streaming server

Microgenomics 2016
The 2nd edition of this unique event will be held once again in Paris, France

Aims and Topics
Today, one of the biggest challenges for biologists is the ability to understand and analyze the genome expressed in a given cell type in the tissue environment, which means you can specifically isolate these cells and work on very small quantities of biological material.

The aim of the congress is to bring together internationally renowned experts in the field and offer an overview of the present knowledge for obtaining high quality molecules and future developments in "omic" tools (DNA, RNA and protein) for genome analysis and its expression at the cell level.

The latest advances in technology in the field will be introduced (flow cytometry, laser capture microdissection, NGS, digital PCR, WGA, LC-MS/MS, RPLA, etc).

The symposium will be divided into four sessions introduced by keynote lecturers, experts in their field :

1- Sampling methods (flow cytometry, laser capture microdissection, DEP array)
2- Microgrenomics and DNA
3- Microgenomics and RNA and small RNA
4- Microgrenomics and Proteins

Symposium Venue -- Institut Pasteur

The symposium will be held on June 2-3 2016 at the Institut Pasteur Conference Center, 25-28 rue du Docteur Roux 75015 Paris. The Conference Center, offers vast spaces for conferences, sponsoring and lunches only a short walk away from the Tour Montparnasse in Paris’ very lively 15th district.

Join Agilent Technologies at their first European Animal and Plant Symposium which will be hosted in Amsterdam, on February 25th and 26th 2014.

Location:     De Rode Hoed
                     Keizersgracht 102
                     Amsterdam - The Netherlands

Abstract Submission - We need you!
If you are interested to submit a presentation, please send us a 200 word abstract no later than January 31st 2014.

Download agenda:

Advances in qPCR & dPCR, 14-15 May, Barcelona, Spain

SELECTBIO is pleased to present the 5th Advances in qPCR conference. In line with this evolving field, special focus will also be given to dPCR at this event.

New developments in qPCR technology will be showcased, concentrating on improvements to qPCR design, the acquisition of accurate data and efficient data analysis. Focus will also be given to the use of dPCR in the clinic, covering applications such as infectious disease diagnostics and cancer detection. Attending this event will provide you with many opportunities to network with peers, helping you to build new relationships and optimise your workflow.

Agenda Topics:

  • Clinical Applications of qPCR & dPCR
  • Mutation Analysis
  • New Developments in Digital PCR
  • Single Cell Analysis
  • Standardisation & Validation

Keynote Speakers:

Mikael Kubista
Professor/Founder, TATAA Biocenter

Carl Wittwer
Professor, University of Utah

Running alongside the Advances in qPCR & dPCR conference will be the ELA exhibition, covering the latest technological advances and associated services from leading solution providers within this field. Registered delegates will also have access to the following co-located conference tracks, ensuring a cost effective trip: Advances in NGS & Big Data, High Content Analysis, Advances in Automation & Robotics, Advances in Cellular Assays & Cell Culture.

qPCR Seminars and Education Programs:

Free of charge web seminars

Sigma-Aldrich is pleased to be able to bring you a series of webex seminars dedicated to qPCR. They will include technical presentations from international speakers discussing the latest techniques and aspects of qPCR. To register, go to QPCR Seminars
Sigma-Aldrich has the pleasure of inviting you to a seminar series devoted to the technical aspects of qPCR and RT-qPCR.

Expert speakers will cover areas such as:
  • QPCR assay design and optimisation
  • Template handling and purification
  • Focus on the optimisation of RT-qPCR
  • Various approaches to genetic analysis
  • Working with limiting samples
  • Data analysis
  • Troubleshooting
  • Assay improvement option for challenging assays
  • LNA technology and application
Seminar grading key:
  • Level 1 - terrified and beginners
  • Level 2 - something for everyone but a background knowledge is assumed
  • Level 3 - improvers, seminars focused on specific areas of interest
  • Level 4 - brave and experienced, delving into the fine detail and the more troublesome aspects of the technique
Available Seminars:

Author Seminar Title
Mudassir Mohammed, Ph.D. An Introduction to qPCR
Valerie Hawkes, Ph.D. Assay Design and Optimisation
Neven Zoric, M.Sc. Approaches to Normal qPCR — Facing the Challenge of Normalization
Tania Nolan, Ph.D. Deriving a Troubleshooting Protocol
Mikael Kubista, Prof. A Statistical Approach to qPCR Gene Expression Data Analysis
Marina Tshuikina, Ph.D. An Introduction to Epigenetics Analysis by qPCR
Valerie Hawkes, Ph.D. Improving the Discrimination and Sensitivity of qPCR Assays using LNA
Chris Bass, Ph.D. Development of DNA-based Diagnostic Assays for Sensitive Screening of Mosquito Disease Vector Populations
Michael Pfaffl, Ph.D. Influence of RNA Integrity on Real-Time RT-PCR Quantification Data
Vladimir Benes microRNA Profiling
Rebecca Hands Technical Tips on LCM Extraction for mRNA Profiling
Jens Stolte cDNA Amplification with GenomePlex® Complete Whole Genome Amplification Kit
Tanya Novak, M.Sc. The SPUD Assay Has an Important Role in the Interpretation of Detecting Pneumocystis DNA in Clinical Specimens

New seminars

This six webinar series on PCR will be based on MIQE guidelines. MIQE, Minimum Information for Publication of Quantitative-Real Time PCR, helps scientists to design and report reliable and reproducible qPCR results. They help others to understand and replicate already conducted experiments. In this webinar series we will discuss step by step critical points which need to be taken into consideration in order to acquire reliable data.

Topics will include:

  • Basic principles of  PCR, qPCR and ddPCR
  • qPCR Assay Design
  • Importance of Sample Quality in qPCR Analysis
  • Improving Assay Quality by Optimization and Validation
  • Data Analysis
  • Troubleshooting
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