real-time qPCR workshops
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WorkshopsSymposia, Meetings, Conferences,  EMBO  & EMBL  events,  Seminars, qPCR Education Programs, ...

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Upcoming events:
  previous qPCR events:
qPCR 2004   qPCR 2005   qPCR 2005 in Leipzig   qPCR 2007    qPCR 2009    qPCR 2010 in Vienna  qPCR 2007 in US   qPCR 2008 in US qPCR 2009 in US  qPCR 2010 in Gothenburg  qPCR 2010 in US   qPCR 2011  qPCR 2011 in Prague   qPCR & NGS 2013

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qPCR & NGS 2015


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280 talks recorded at
qPCR 2010 &
qPCR 2011
qPCR & NGS 2013 & 2015


We provide the presentations via movie streaming technology in high quality - high resolution and perfect sound quality in high speed - on any internet browser or mobile device, including Android or iOS.

qPCR Workshops:

qPCR curses are held regularly in Göteborg Sweden and Prague Czech Republic

Our highly appreciated courses are suitable for researchers, technicians, post-graduate students or anyone that wants to learn more about real-time PCR and real-time PCR related technologies. Most of our courses have both theoretical and practical parts where the participants get to do experiments themselves under experienced supervision. Different course modules are available at different occasion and you may take a full week course or just a single course module  =>  qPCR course event calendar

Course modules:

3 days Hands-on qPCR 2 days Hands-on qPCR  1 day Sample preparation

3 days Exp design & data analysis 2 days Exp design & data analysis  1 day Immuno-qPCR

2 days Single cell analysis
 1 day Multiplex PCR

 1 day HRM

 1 day MicroRNA analysis

 1 day Quality control in MDx

2-day qPCR courses


Entire qPCR workflow covered in two days . Focus on gene expression (including microRNA) . Theoretical background and practical hands-on data-analysis.Complemented with training on Biogazelle’s qbase+ software . Focus on experiment design and data-analysis . Attention for validation and quality control


1- Throughout the course the trainers explain how quality control at each step in the qPCR workflow ensures accurate results.

2- A reliable qPCR study begins with good experiment design. On the first day of the course the principles of experiment design are complemented with practical exercises.

3- Normalization using a non-validated housekeeping gene may lead to errors more than 3-fold in 25% of the experiments. To avoid such errors, the students learn how to determine which and how many reference genes to use.

4- Statistical analysis of qPCR results remains a bottleneck for many researchers. The course covers descriptive statistics to summarize qPCR results; key guidelines are provided to facilitate the selection of appropriate statistical tests.

5- Biogazelle’s qPCR course allows the students to master the MIQE guidelines and publish their qPCR results accordingly.


Interactive training by qPCR experts with proven track record, a 2-month premium qbase+ license, a package of references and course notes on qPCR design and analysis. The course is catered (lunch and pauses) on both days.

TARGET AUDIENCE:   The course is intended for all qPCR users with a basic level of experience.


More information of qbase+ software and Biogazelle:

EMBL - Courses, Conferences and Workshops

Extracellular Vesicles Purification, Identification and Translation

EMBL Heidelberg, Germany
30 Nov - 3 Dec 2015

In the past few years extracellular vesicles (EV) have increasingly attracted the attention of scientists worldwide. Recent research has shown that these small vesicles act as messengers conveying information not only to neighboring cells, but also to distant tissues in eukaryotes, and between cells in prokaryotes. Containing cell-specific payloads of proteins, lipids and genetic material, they are able to alter function and physiology of target cells. Their importance in pathophysiological processes is just beginning to unravel and thus, also their biomedical relevance.

A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles (with practical sessions included).

  • Extracellular vesicles purification (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques)
  • Extracellular vesicles characterisation (Immunoblot and electron microscopy, Quantitative and qualitative analysis by flow cytometry, Structural and mechanical characterisation by atomic force microscopy, Nanoparticle Tracking Analysis)
  • Extracellular vesicles composition (Lipidomics, Proteomics, Transcriptomics)
  • Extracellular vesicles biogenesis and function (Molecular players for extracellular vesicles biogenesis, Extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)
Who should apply?
This course is intended for PhD and post-doctoral researchers entering the field.

Principles, parameters and applications of PCR

Date: 7 April 2015
Location: Avans Hogeschool Breda, The Netherlands
Registration fee: €400,-

Real-time PCR in molecular diagnostics
Date: 8-10 April 2015
Location: Avans Hogeschool Breda, The Netherlands
Registration fee: €900,-


Introduction to Next Generation Sequencing; technologies and data analysis
Date: 14-17 April 2015
Location: Avans Hogeschool Breda, The Netherlands
Registration fee: € 1100,-


qPCR Symposia, Meetings, and Conferences:

Microgenomics 2016
The 2nd edition of this unique event will be held once again in Paris, France

Aims and Topics
Today, one of the biggest challenges for biologists is the ability to understand and analyze the genome expressed in a given cell type in the tissue environment, which means you can specifically isolate these cells and work on very small quantities of biological material.

The aim of the congress is to bring together internationally renowned experts in the field and offer an overview of the present knowledge for obtaining high quality molecules and future developments in "omic" tools (DNA, RNA and protein) for genome analysis and its expression at the cell level.

The latest advances in technology in the field will be introduced (flow cytometry, laser capture microdissection, NGS, digital PCR, WGA, LC-MS/MS, RPLA, etc).

The symposium will be divided into four sessions introduced by keynote lecturers, experts in their field :

1- Sampling methods (flow cytometry, laser capture microdissection, DEP array)
2- Microgrenomics and DNA
3- Microgenomics and RNA and small RNA
4- Microgrenomics and Proteins

Symposium Venue -- Institut Pasteur

The symposium will be held on June 2-3 2016 at the Institut Pasteur Conference Center, 25-28 rue du Docteur Roux 75015 Paris. The Conference Center, offers vast spaces for conferences, sponsoring and lunches only a short walk away from the Tour Montparnasse in Paris’ very lively 15th district.

The comprehensive meeting will focus on new developments in qPCR technology, concentrating on improvements to qPCR design, the acquisition of accurate data, and efficient data analysis. Particular emphasis will also be given to the use of qPCR in Cancer diagnosis and the use of dPCR in the clinic, covering applications such as infectious disease diagnostics and cancer detection.
We will examine the latest developments in sequencing platforms and methods. Focus will be given to RNA sequencing, as well as emerging clinical applications of NGS. Presentations will also highlight the use of bioinformatics and data analysis. In addition to the extensive conference programme, we will also be holding a pre-conference Training Workshop in conjunction with TATAA Biocenter, Sweden. 

We are pleased to introduce the following highly distinguished experts who comprise the Organising Committee for 2016
  • Michael Pfaffl, Professor, Technical University Munich
  • Mikael Kubista, CEO/Founder TATAA Biocenter, Professor Institute of Biotechnology of the Czech Academy of Sciences
  • Fred Kramer, Professor, New Jersey Medical School, Rutgers University
  • Olivier Thas, Professor in Biostatistics & Honorary Professor, Ghent University & University of Wollongong, Australia
  • David Ruff, Principal Scientist, Fluidigm Corporation
  • Alexander Lezhava, Group Leader, Genome Institute Singapore (GIS)
Attending this meeting will provide you with an excellent opportunity for networking with the world’s leading experts, helping you to find unique solutions and build long term collaborations.

20th World Congress on Advances in Oncology 
18th International Symposium on Molecular Medicine

October 8-10, 2015
Metropolitan Hotel ***** Athens, Greece

The continued interest and increasing support that our annual Congress on Advances in Oncology and Symposium of Molecular Medicine has gained over the years, motivates us to repeat this success this year in October 2015. Over the last 20 years we have consistently welcomed a broad international audience, which surpassed 500 participants in 2014.

The 20th World Congress on Advances in Oncology and the 18th International Symposium of Molecular Medicine will take place from 8-10 October, 2015.
The event will be held at the Metropolitan Hotel ***** Conference Center in Athens, Greece.

The aim of our conferences is to keep participants up to date with the latest scientific developments in Oncology and Molecular Medicine. Our conferences constitute a unique venue for basic and clinical research for the development of molecular diagnostics and cancer therapeutics. Some of the many topics that will be discussed are:

  • Biochemistry in Molecular Oncology
  • Immunology in Molecular Oncology
  • Experimental Carcinogenesis
  • Apoptosis/Hypoxia
  • Chemotherapy/Pharmacology
  • Tumor Microenvironment & Signaling
  • Cancer Inflammation & Differentiation
  • Cancer Therapeutics

  • Molecular Medicine
  • Molecular & Clinical Diagnostics
  • Quantitative PCR & Next Generation Sequencing
  • Discovery and Validation of Biomarkers
  • CTCs  &  Circulating Biomarkers
  • Regulative small RNAS in Cancer
  • … … and additional subjects relating to Oncology and Molecular Medicine.

In parallel to the scientific symposium of 2015, an Industrial Exhibition will take place, at which international companies, leaders in the fields of Oncology and Molecular Medicine, will present their latest technologies and services in clinical diagnostics. If you are interested in joining the exhibition, please contact us at

Further, two qPCR Workshops dedicated to ‘Molecular Diagnostics’, Biomarker Development’ and ‘qPCR Standardization’ in applying the MIQE guidelines, will take place before the symposium on 6-7 October (hosted by TATAA Biocenter Sweden).
  • Discovery and Validation of Expression Biomarkers - Experimental Design and Data Analysis
  • MIQE guidelines - How to get good quality control in qPCR
We look forward to your participation.

Yours sincerely,

Demetrios A. Spandidos

qPCR & NGS 2015 Event
7th international qPCR & NGS Event -- Symposium  &  Industrial Exhibition  &  Application Workshops
23-27 March 2015, in Freising-Weihenstephan, TUM School of Life Sciences
Technical University of Munich, Weihenstephan, Germany

Central topic:   Advanced Molecular Diagnostics for Biomarker Discovery

Symposium Talk & Poster Sessions:

•    Main topic:   Advanced Molecular Diagnostics
•    Main topic:   Biomarker Discovery
•    Main topic:   Next Generation Sequencing (NGS)
    - NGS overview talks - information technology in the era of NGS
    - Pre NGS - sample prep & setup & library generation
    - NGS – new sequencing technologies (e.g. single molecule and pore sequencing)
    - NGS – data analysis (data management, mapping, alignment algorithms, data de novo assembly )
    - NGS – diagnostic applications

•    MicroGenomics & Single-cells diagnostics
•    Circulating Nucleic Acids
•    Molecular Diagnostics in Agriculture, Veterinary Medicine, Food & Environmental Science
•    MIQE & QM & Standardisation strategies in molecular diagnostics
•    Digital PCR  &  Nano-fluidics
•    Non-coding RNAs -- microRNA, small RNAs, long non-coding RNAs
•    qPCR Data Analysis -- BioStatistics & BioInformatics

Scientific board:  Stephen Bustin, Mikael Kubista, Vladimir Benes, Jim Huggett, Jo Vandesompele, Michael W. Pfaffl

Regsiter for the symposium and the workshops =>
Download the event flyer =>
Event location on Google maps

qPCR & NGS 2015
Scientific coordination: Michael W. Pfaffl
Event organization: Sylvia Pfaffl bioMCC

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qPCR Seminars and Education Programs:

Free of charge web seminars

Sigma-Aldrich is pleased to be able to bring you a series of webex seminars dedicated to qPCR. They will include technical presentations from international speakers discussing the latest techniques and aspects of qPCR. To register, go to QPCR Seminars
Sigma-Aldrich has the pleasure of inviting you to a seminar series devoted to the technical aspects of qPCR and RT-qPCR.

Expert speakers will cover areas such as:
  • QPCR assay design and optimisation
  • Template handling and purification
  • Focus on the optimisation of RT-qPCR
  • Various approaches to genetic analysis
  • Working with limiting samples
  • Data analysis
  • Troubleshooting
  • Assay improvement option for challenging assays
  • LNA technology and application
Seminar grading key:
  • Level 1 - terrified and beginners
  • Level 2 - something for everyone but a background knowledge is assumed
  • Level 3 - improvers, seminars focused on specific areas of interest
  • Level 4 - brave and experienced, delving into the fine detail and the more troublesome aspects of the technique
Available Seminars:

Author Seminar Title
Mudassir Mohammed, Ph.D. An Introduction to qPCR
Valerie Hawkes, Ph.D. Assay Design and Optimisation
Neven Zoric, M.Sc. Approaches to Normal qPCR — Facing the Challenge of Normalization
Tania Nolan, Ph.D. Deriving a Troubleshooting Protocol
Mikael Kubista, Prof. A Statistical Approach to qPCR Gene Expression Data Analysis
Marina Tshuikina, Ph.D. An Introduction to Epigenetics Analysis by qPCR
Valerie Hawkes, Ph.D. Improving the Discrimination and Sensitivity of qPCR Assays using LNA
Chris Bass, Ph.D. Development of DNA-based Diagnostic Assays for Sensitive Screening of Mosquito Disease Vector Populations
Michael Pfaffl, Ph.D. Influence of RNA Integrity on Real-Time RT-PCR Quantification Data
Vladimir Benes microRNA Profiling
Rebecca Hands Technical Tips on LCM Extraction for mRNA Profiling
Jens Stolte cDNA Amplification with GenomePlex® Complete Whole Genome Amplification Kit
Tanya Novak, M.Sc. The SPUD Assay Has an Important Role in the Interpretation of Detecting Pneumocystis DNA in Clinical Specimens

New seminars

This six webinar series on PCR will be based on MIQE guidelines. MIQE, Minimum Information for Publication of Quantitative-Real Time PCR, helps scientists to design and report reliable and reproducible qPCR results. They help others to understand and replicate already conducted experiments. In this webinar series we will discuss step by step critical points which need to be taken into consideration in order to acquire reliable data.

Topics will include:

  • Basic principles of  PCR, qPCR and ddPCR
  • qPCR Assay Design
  • Importance of Sample Quality in qPCR Analysis
  • Improving Assay Quality by Optimization and Validation
  • Data Analysis
  • Troubleshooting
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