qPCR Workshops:
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Our highly appreciated courses are suitable for
researchers,
technicians, post-graduate students or anyone that wants to learn more
about real-time PCR and real-time PCR related technologies. Most of our
courses have both theoretical and practical parts where the
participants
get to do experiments themselves under experienced supervision.
Different course modules are available at different occasion and you
may take a full week course or just a single course module
=> qPCR
course event calendar
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3-day
hands-on workshop with extensive data analysis and troubleshooting
sessions will be held from 23rd to 25th October 2013 in Munich,
Germany. Workshop is organised by BioSistemika Ltd. in collaboration
with Technical University of
Munich
and BioM Biotech Cluster
Development GmbH
qPCR
Experience® workshop: Real-time PCR in Gene Expression Studies
includes qPCR theory review (principles and applications, chemistries
and equipment) and the practical sessions. Practical part of the
workshop will cover practical guidance on sample preparation and
quality control, assay design, experiment setup, relative
quantification strategies and normalization, practical data analysis
(ddCq), primer design, MIQE guidelines etc. (See the program below)
Guest
Lecturer
Prof.
Dr. Michael W. Pfaffl -
Professor of Molecular Physiology at the Technical University of Munich
in Freising. He has reached the “Principal Investigator” status at the
Institute of Physiology and is one of the leading scientists concerning
qPCR technology, relative quantification, and RNA expression profiling.
Join our international group and
exquisite lecturers in Munich in
October and learn about new tips & tricks
for even better results
in gene expression analysis using qPCR technology!
More information about
past qPCR Experience® workshops, can be found HERE
If you would like to read testimonials and opinions about qPCR
Experience® workshops, please visit Testimonials
Programme
(please
note that minor changes in the workshop program might occur, this is a
preliminary version)
Day 1 - 23rd October 2013 (10 am - 5 pm)
- Arrival & registration
- Introduction
- Real-time PCR principles and applications
- Review of real-time PCR chemistries and
equipment
- Experiment and laboratory workflow
- Sample preparation
- Sample preparation -blood
- Experiment setup - gene expression experiment
- GUEST LECTURES: Michael W. Pfaffl: "RNA
integrity" and "Absolute Quantification"
- Welcome reception
Day
2 - 24th October 2013 (9 am - 5 pm)
- Instrument setup and preliminary data analysis
- Relative quantification strategies and
normalization
- Data analysis practical - ddCq
- Primer design
- Assay validation
- MIQE guidelines
Day
3 - 25th October 2013 (9 am- 5 pm)
- Large dataset analysis practical
- Troubleshooting
- Future of real-time PCR
- Product presentation
- Wrap-up and discussion
- The end
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qPCR
Application Workshops
held at the qPCR & NGS Event in March 2013
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Analytik
Jena bietet Ihnen mit Seminaren, Workshops und individuellen
Trainings genau die flexible und umfassende Unterstützung, die Sie
benötigen, um effektiv und kostengünstig zu arbeiten. Betreut
durch
erfahrene Applikations- und Servicespezialisten, sind die
Veranstaltungen die richtige Plattform für Weiterbildung und
Erfahrungsaustausch.
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Anwenderseminar
Real-Time PCR (Grundlagenkurs)
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- Real-Time
Chemie
- Auswerte-Methoden
- High-End
Gerätetechnik im Vergleich
- Anwendung
verschiedener Ready-to-Use SYBR®Green Kits
- Verschiedene
Auswerte-Methoden in der Anwendung
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Termin
25.04.2013 in Jena
Gebühr
395,00 EUR
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Praxisseminar
Real-Time PCR
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- Methodenentwicklung
und -optimierung
- Praktische
Messungen am Gerät
- Wartung
und Pflege des Gesamtsystems
- Troubleshooting
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Termin
26.04.2013 in Jena
Gebühr
395,00 EUR
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Real-Time
PCR kompakt!
Nehmen Sie teil am Grund- und Praxisseminar.
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Sonderpreis
700,00 EUR |
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Praxisseminar
Manuelle Nukleinsäureextraktion
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- Grundlagen
der Nukleinsäureaufreinigung
- Theorie
der Nukleinsäureextraktion
- Homogenisierungsoptimierung
- Kits in
der Anwendung
- Nukleinsäureanalytik
- Nanospektrophotometrische
Messungen
- Elektrophoretische
Trennung
- Fluoreszenzbasierte
Analysen
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Termin
23.04.2013 in Jena
Gebühr
395,00 EUR
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qPCR Experience Boot Camp , Piran,
Slovenia, 11. - 14. September 2012
Focus: Hands-on real-time PCR workshop covering
complete laboratory and data analysis workflow with extensive practical
sections in ISO accredited labs of NIB.
Invited speaker:
Dr. Stephen A. Bustin, professor of molecular
science at Barts and the London School of Medicine and Dentistry, Queen
Mary University of London, UK, a trendsetter in qPCR.
Duration: 4 days
qPCR data analysis
strategies for different applications, Maribor, Slovenia, 26. September
2012
Focus: Workshop is a satellite workshop of Genetics
2012 Congress. It will focus on data analysis strategies for different
applications of qPCR (diagnostics, gene expression studies, SNPs
detection). It includes hands-on data analysis session.
Invited speaker:
Dr. Natasa Toplak, Omega Ltd, expert in various qPCR
research and diagnostic applications.
Duration: 1 day
qPCR in Plant Pathology
diagnostics, Ljubljana, Slovenia, 4. – 7. December 2012
Focus: Hands-on workshop will cover real-time PCR in
diagnostics in the field of plant pathology with emphasis on quality
control of the complete workflow and extensive practical part in ISO
accredited labs of NIB.
Invited speaker:
Dr. Neil Boonham, the team leader at Fera (The Food
and Environment Research Agency, York, UK) and world known specialist
in plant pathogens diagnostics.
Duration: 4 days
For more
information of workshops please visit http://www.biosistemika.com/workshops
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Microbial
Molecular Profiling and
Identification Workshop
LGC, Teddington, UK. April 19th
2013. This will:
- Focus on 16S analysis by NGS
- Applications including
metagenomics (clinical, industrial and environmental), diagnostics and
epidemiology
- Standardisation - reference
materials
For further information please contact jim.huggett@lgcgroup.com
Digital PCR training – principles and current applications
LGC, Teddington, UK. April 18th 2013, this one
day course
will:
- Provide an overview of dPCR
technology and it’s applications
- Give guidance on dPCR
experimental design
- Illustrate current approaches
to dPCR data analysis
- Provide advice on commonly
encountered problems
download
PDF flyer
For
further information please contact jim.huggett@lgcgroup.com
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The course
Principles, parameters and
applications of PCR
October 21, 2013
http://www.alsavans.nl/avans-training-2545.htm
Aim of the course:
This short course aims at training the participant in the basic
principles and applications of the Polymerase Chain Reaction (PCR) and
real-time PCR. The program consists of a series lectures and
experiments covering all basic PCR topics like efficiency, fidelity and
specificity of the PCR reaction.
Participants:
This course is developed as an introduction to the PCR and especiall
suitable as a basic introduction to our advanced course on Real-time
PCR in molecular diagnostics (2548). Basic knowledge of molecular
biology in general is desirable.
Course content:
Theory:
Principles of DNA amplification technologies; The biochemical and
physical aspects of the PCR
Principles, parameters, and design of (real-time) PCR primers
Principles and parameters of detection of PCR products
Principles of Real-time PCR; detection formats, assay formats
PCR contamination prevention
Practicals:
Real-time PCR dilution series and melting curve analysis:
Optimization and determination of the sensitivity of real-time PCR with
SybrGreen
Optimization and determination of the sensitivity of real-time PCR with
Hybridisation probes
The
course Real- time PCR in molecular
diagnostics
October 22,23 and 24, 2013
http://www.alsavans.nl/avans-training-2548.htm
Aim of the course:
Real time Polymerase Chain Reaction is becoming more and more important
in almost all laboratories. Especially in diagnostic laboratories real
time PCR is a new, and promising, technique to identify bacteria,
viruses and genetic alterations in human cells. The aim of this
international short-course is to give the participants an in-depth view
in the theoretical and practical knowledge of real time PCR techniques
in molecular diagnostics. State-of-the-art experts in the field of real
time PCR techniques from several diagnostic laboratories will provide
theoretical and practical expertise.
Participants:
This course is developed for experienced laboratory technicians
involved in the life sciences laboratories, incl. medical diagnostics,
microbiology, genetics, pathology, immunology, etc.
Basic knowledge of molecular biology in general and PCR is a
prerequisite. If you feel you lack the basic knowledge in PCR or the
basic principles of real-time PCR, but you would like to participate in
this quantitative PCR course, please note that the Avans Hogeschool
also offers an international introductory short course: Principles,
parameters, and applications of PCR (code 2545), which is especially
suitable as a basic introduction to this course.
Course structure:
The course consists of a series of practical sessions interspersed with
lectures and tutorials. 50% of the time is devoted to lectures and 50%
is spent on "hands-on" experiments, covering techniques that may be
directly applied in diagnostic laboratories. All participants will
receive a practical manual containing all used protocols.
Course content:
Day 1: Pre-PCR: Sample preparation and RNA isolation for real-time PCR
and HRMA
Day 2: Real-time PCR and validation
Day 3: HRMA, digital PCR and Post-qPCR data analysis
Theory:
Sample preparation for real-time PCR
RNA quality control (Lab-on-a-chip, RIN)
Real-time quantitative PCR and mathematics
Trouble shooting and discussion of qPCR problems
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qPCR Symposia, Meetings, and
Conferences:
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On
behalf
of
the Organisation Committee and the Scientific Board it is
a great pleasure to invite you to the 6th International qPCR
& NGS 2013 Event. The event is divided in a 3-day scientific Symposium with an Industrial
Exhibition
and various 2-day Application
Workshop to
be
held
at
the Center of
Life Science in Freising Weihenstephan,
Technische Universität München (Germany).
The
great
international
interest
in the previous meetings [ qPCR
2004 qPCR 2005 qPCR
2005 in Leipzig qPCR
2007 qPCR 2009
qPCR
2010 in Vienna and qPCR 2011 ] led us
to the decision to repeat the
Symposium in
spring 2013. We expect 500-600
participants
coming from all over the world,
in 2011 we could welcome participants from 56 contries, and roughly 30-40
international companies in
the qPCR Industrial Exhibition.
We have
set the date for the qPCR & NGS
2013 Event
to 18th
- 22nd March 2013. The event location is the central lecture hall
complex
and the foyer at TUM (Technical University of Munich) in Freising
Weihenstephan, Germany (download Google Maps link or Google
Earth link).
The TUM and the Biotech region around Munich is
part of the largest Biotech cluster in Europe, located close to the
Munich airport in the heart of Bavaria.
The focus of the qPCR 2013 Event will be on:
Next Generation
Thinking in Molecular Diagnostics
Leading
academic researchers and
industrial contributors in the field will participate in the
symposium, which will be an arena for fruitful discussions between
researchers of different backgrounds. The Symposium Talks, Poster
Sessions, Industrial
Exhibition and associated qPCR & NGS
Application
Workshops offer an
overview of
the present knowledge and future developments in qPCR, next generation
sequencing and gene
expression measurement technology and
its wide applications in research.
The
symposium
will
focus on 74
lectures and a huge
poster exhibition (105 posters) will
be presented
by
internationally
recognised experts in their field. The emphasis will be
on unbiased, didactic
information exchange. Internationally
reknown speakers will be participating in a lively and exciting
programme enabling the valuable exchange of information in the qPCR
field. One
third
of
the talks
will
be
presented by selected invited speakers,
one
third will be selected from the submitted
abstracts and one
third will be presented by qPCR related company
R&D
representatives. All
scientific contributions will be published
in the qPCR
2013
Symposium
Proceedings (ISBN to be announced).
Full
papers from
selected invited academic and industrial speakers and
application notes from industrial
speakers will
be published in a METHODS special
issue“Transcriptional
Biomarkers” edited
by Michael W. Pfaffl (published
January 2013). At
the meeting all participants will receive a print copy of this special
Methods issue "Transcriptional
Biomarkers" METHODS 2013 59(1) pages 1-192
Symposium
Agenda is available - 74 talks and 105 posters accepted !
HTML
version of the agenda
PDF version
of the Agenda Summary of the Poster
Presentations
Symposium
Talk
and Poster sessions:
- Main
Topic: Molecular
diagnostics
- Main Topic: Next
Generation Sequencing (NGS)
- Main Topic: Transcriptional
Biomarkers
- High
throughput
analysis
in
qPCR
- Systems
biology
- Single-cells diagnostics
- MIQE & QM
strategies in qPCR
- non-coding RNAs -
microRNA, siRNA
and long non-coding RNAs
- Digital PCR
- Lunch
Time Seminars
- Biogazelle - qBASEplus - qPCR data analysis
seminar
- MultiD - GenEx - qPCR data analysis seminar
- Life TEchnologies - digital PCR seminar
- Genomatix - NGS data analysis seminar
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The forum „Trends in
Microanalytical Systems and Chip-based Bioanalysis“ will be organized
by Lausitz University of Applied Sciences, BioResponse e. V. together
with the Center for Molecular Diagnostics and Bioanalysis (ZMDB) as the
7th “Senftenberger Innovationsforum Multiparameteranalytik”. The
scope of the workshop comprises microanalytical systems and chip-based
bioanalytics with a focus on the real-time monitoring of amplification
reactions and related multiplex technologies. We encourage also a
submission of abstracts on new analytical technologies, applications,
and instrumentation, instrumental cell based bioanalytics, use of
micro/nano-structured materials, and micro- and nanoparticles and
microdroplets.
The workshop will include oral lectures, poster
presentations, an industrial exhibition (Alere Technologies GmbH,
Analytik Jena AG, Attomol GmbH, IBA GmbH, Medipan GmbH, PolyAn GmbH,
Scienion AG) and demonstration of prototypes of analytical devices
(Mainz Institute for Microtechnology, Fraunhofer Institute for
Biomedical Engineering, Lausitz University of Applied Sciences).
The format of the conference intends active participation and
interaction of experts from research institutions and industry.
The participation is free of charge. Prior registration is requested
Call for Papers: Selected papers will be published in a special issue
of Microchimica Acta. The selection will be performed by peer review
procedure. Enquires concerning paper submission:
mca-specialissue@hs-lausitz.de. Deadline for abstract submission: March
1st, 2013.
Call for Posters/Call for Presentations: You are kindly invited to
present results of your research or new products. We accept abstracts
for talks and posters. A prize for the best posters will be awarded.
Deadline for abstract submission: March 1st, 2013. Please contact
Christine Mißler (missler@zmdb.de).
Confirmed Speakers
• Agata Jósefiak - Centrum
Badan DNA Sp.z.o.o. Poznan (Poland)
• Andrew deMello - ETH Zürich
(Switzerland)
• Dirk Kuhlmeier -
Fraunhofer-Institut für Zelltherapie und Immunologie IZI (Germany)
• Dirk Laßner - Institut
Kardiale Diagnostik und Therapie GmbH Berlin (Germany)
• Frank T. Hufert -
Universität Göttingen (Germany)
• Ingo Klimant - Technische
Universität Graz (Austria)
• Karina Weber - Institut für
Photonische Technologien (IPHT) &
Friedrich-Schiller-Universität Jena (Germany)
• Michael W. Pfaffl - Technische
Universität München (TUM) (Germany)
• Oliver Strohmeier - (HSG-IMIT)
(Germany)
• Ralf Ehricht - Alere
Technologies GmbH (Germany)
• Ralf Himmelreich - IMM Institut
für Mikrotechnik Mainz GmbH Mainz (Germany)
• Sebastian Kersting -
Fraunhofer-Institut für Biomedizinische Technik IBMT (Germany)
• Simon Wadle - (HSG-IMIT)
(Germany)
• Ute Resch-Genger - BAM
Bundesanstalt für Materialforschung und -prüfung (BAM)
(Germany)
• Werner Lehmann - Attomol GmbH
(Germany)
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TATAA Biocenter and PhileKorea Technology
proudly presents and welcomes you to the qPCR and Next Generation
Sequencing symposium of 2012
taking place in Daejeon, South Korea, November
13th-16th, 2012
The
symposium
will consist of days with scientific sessions and
industrial exhibition and also symposium workshops.
Besides interesting talks about the new developments in real-time
qPCR
and Next Generation Sequencing (NGS) research, special focus in this
symposium is:
Symposium Topics:
* Molecular diagnostics
* Standardization and quality control
* Preanalytics: sample preparation, handling and
storage of clinical specimens
* Molecular Biomarkers
* High throughput expression profiling
* Epigenetics, mutation analysis and copy number
variation
* Single-cell and subcellular expression profiling
* Non coding RNAs: RNAi and miRNA
* Detection and characterization of tumor cells
* qPCR and NGS in Forensics and AgriBio
* Experimental design and data mining
* Clinical applications of qPCR and NGS
* Digital PCR
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Single-Cell
Gene Expression Analysis
EMBO Practical Course
Single-Cell Gene Expression Analysis
EMBL Heidelberg, Germany Saturday 29 June - Friday 5 July 2013
The analysis of transcripts in minute samples is often used to increase
the knowledge in areas such as cancer development, plant physiology,
neurodegenerative disorders of developmental biology. A very precise
sampling crucial is crucial for the success of the approach. Depending
on the nature of the sample and the goal of the experiment, there are
different ways of sampling the probes. This course will present flow
cytometry and laser capture microdissection as methods to obtain single
cells for the subsequent gene expression analysis. The need for
quantitative single-cell mRNA analysis is given by the inability of
conventional RNA methods, to differentiate mRNA abundance of such
limited samples. Another crucial step is data analysis, which
constitutes another strong component of the course.
This course targets scientists who are entering the field of single
cell analysis and want to learn about different preparation methods and
gene expression profiling. The participants will learn about the
complete workflow and gain understanding about the possibilities and
limitations of the approach. The course will cover lectures and
hands-on sessions by experts in the fields and is aimed for PhD
students, postdoctoral fellows or other scientists with a solid qPCR
and molecular biology background.
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qPCR
Seminars and Education Programs:
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Free
of charge web
seminars
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
Expert
speakers
will cover areas such as:
- QPCR assay design and
optimisation
- Template handling and
purification
- Focus on the
optimisation of RT-qPCR
- Various approaches to
genetic analysis
- Working with limiting
samples
- Data analysis
- Troubleshooting
- Assay improvement
option for challenging assays
- LNA technology and
application
Seminar
grading key:
- Level 1 - terrified
and beginners
- Level 2 - something
for everyone but a background knowledge is assumed
- Level 3 - improvers,
seminars focused on specific areas of interest
- Level 4 - brave and
experienced, delving into the fine detail and the more troublesome
aspects of the technique
| Available
Seminars: |
| Author |
Seminar
Title |
| Mudassir Mohammed, Ph.D. |
An Introduction to qPCR |
| Valerie Hawkes, Ph.D. |
Assay Design and
Optimisation |
| Neven Zoric, M.Sc. |
Approaches to Normal qPCR —
Facing the Challenge
of Normalization |
| Tania Nolan, Ph.D. |
Deriving a Troubleshooting
Protocol |
| Mikael Kubista, Prof. |
A Statistical Approach to
qPCR Gene Expression
Data Analysis |
| Marina Tshuikina, Ph.D. |
An Introduction to
Epigenetics Analysis by qPCR |
| Valerie Hawkes, Ph.D. |
Improving the
Discrimination and Sensitivity of
qPCR Assays using LNA |
| Chris Bass, Ph.D. |
Development of DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito Disease Vector Populations |
| Michael Pfaffl, Ph.D. |
Influence of RNA Integrity
on Real-Time RT-PCR
Quantification Data |
| Vladimir Benes |
microRNA Profiling |
| Rebecca Hands |
Technical Tips on LCM
Extraction for mRNA
Profiling |
| Jens Stolte |
cDNA Amplification with
GenomePlex® Complete
Whole Genome Amplification Kit |
| Tanya Novak, M.Sc. |
The SPUD Assay Has an
Important Role in the
Interpretation of Detecting Pneumocystis DNA in Clinical Specimens |
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Stratagene
- Fast Track
qPCR Education Program
http://www.stratagene.com/tradeshows/fasttrack.aspx |
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The Fast Track QPCR
Education Program is a comprehensive program of user training and
advanced education for users of our real-time PCR instruments. The
program provides a wide range of tools to get you up and running with
real-time PCR quickly and effectively. The program also offers you
access to expert Field Application Scientists providing advanced
education. As a leader in the training of scientists on real-time PCR,
we are proud to offer the Fast Track Education Program.
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Fast
Track QPCR Education
Program Benefits:
- Rapid
and effective QPCR training
- Advanced
application-focused education
- On-demand
educational tools
- Ongoing
education to
support new personal and evolving assays
- Consultation
with expert Field Application Scientists
Fast
Track QPCR Education
Program:
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Stratagene
Online Seminars
http://www.stratagene.com/onlineseminars/ |
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Stratagene
offers a variety of live and recorded online seminars
presented by one of our Technical Services Scientists. Simply select a
category of interest below to view the abstracts and to register for a
presentation.
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Technical
Training Courses
http://www.appliedbiosystems.com/service/training/
https://learn.appliedbiosystems.com/
https://learn.appliedbiosystems.com/realtimepcr
https://learn.appliedbiosystems.com/pcr
Basic Real-Time PCR
training EU
(2 days)
Objective:
In this course, the participant is trained on the basic knowledge of
Real-Time PCR and its applications for quantitative and SNP analysis.
With the use of practical examples, this course focuses on the correct
use of the web-based tools for searching genomic TaqMan. assays and the
SDS and the Primer Express. software. In addition, an overview of the
diverse applications of Real-Time PCR is presented.
This
training course is optimized for scientists new to ABI PRISM. Real-Time
PCR platforms and who wish to establish and optimally use the system
for Real-Time PCR and SNP analysis applications.
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