qPCR Workshops:
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Course 2010:
8 - 10 February 2010 (E)
3-day
single-cell &
qPCR (Mon. -
Wed.)
22 -
26 February
2010
(E)
3-day
qPCR Basic
Module (Mon. - Wed.) & 2-day BioStatistics
Module (Thu. -
Fri.)
8 - 9 March 2010 (E)
2-day qPC-R - data
analysis using R packages (Mon.-Tue.)
17 -
21 May
2010
3-day
qPCR Basic
Module (Mon. - Wed.) (G)
& 2-day BioStatistics
Module (Thu. -
Fri.)
(E)
14 - 15 June 2010 (E)
2-day microRNA & qPCR
(Mon.-Tue.)
21 - 22 June 2010 (E)
2-day qPC-R - data
analysis using R packages (Mon.-Tue.)
12 - 15 July 2010
(E)
3-day
qPCR Basic Module (Mon.
- Wed.)
& 1-day HRM
Module (Thu.)
=>
more info HERE
(E)
= English course
(G)
= German course /
deutschsprachiger Kurs
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Open
Courses in
qPCR:
Life Science is
still a growing sector and new methods and technologies are continously
developed. Therefore permanent training and education becomes so
important.
With our specific course program we are offering a range of
high-quality course modules, in cooperation with different companies to
give a general and independent overview of existing qPCR technologies
and systems.
Our course issues are based on skilled know-how from own research
studies and publications.
Our aim is to point out a critical way of thinking to increase the
quality and outcome of experimental data.
=> course
program 2010
=>
register HERE |
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qPCR Symposia, Meetings, and
Conferences:
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The
scientific organization is managed by international well-known
scientists in the field of qPCR:
Stephen
Bustin
Prof. of Molecular Science, School
of Medicine, London, UK
Mikael
Kubista
Prof. of Biotechnology, TATAA Biocenter, Sweden
Christine Mannhalter Prof. for Molecular
Diagnostics in Clinical Chemistry, Med. University Vienna, Austria
Jo Vandesompele
Prof.
at the Center of Medical Genetics, University of Ghent, Belgium
Michael W.
Pfaffl
Senior Scientist and Reader in Physiology, Technical University of
Munich, Weihenstephan, Germany (scientific coordination)
qPCR
Symposium
topic - “The ongoing evolution
of qPCR”
The symposium focus will be on 40 lectures presented by international
recognised experts in their application fields. The emphasis will be on
unbiased, didactic information exchange. One third of the talks will be
presented by invited speakers, one third of the speakers will be
selected from the submitted abstracts and one third will be qPCR
company representatives with their newest qPCR technologies.
The focus of the qPCR 2010 Event will be “The ongoing evolution
of
qPCR” representing all new and emerging techniques,
applications and
data analysis methods:
Symposium
sessions, speakers & preliminary title
MIQE
and QM strategies in qPCR
The MIQE guidelines: minimum information for publication of
quantitative real-time PCR experiments. Following these guidelines will
encourage better experimental practice, allowing more reliable and
unequivocal interpretation of qPCR results. QM strategies in real-time
PCR to guarantee better and more valid results.
Prof.
Stephen Bustin,
“The MIQE Guidelines: Minimum
Information for Publication of Quantitative Real-Time PCR Experiments”
IT-IS
Lifescience, "Optical artefacts: Cause, Consequence,
Correction"
High
throughput quantitative PCR
– digital PCR
384 well applications, new high throughput platforms, droplet PCR, qPCR
robotics, digital PCR, gene expression real-time RT-PCR arrays (mRNA
and microRNA), quantitative multiplexing, …
Prof.
Mikael Kubista,
“Digital PCR and intracellular expression
profiling”
Dr.
Philip Day, “High
throughput droplet PCR”
Dr. Ken Livak,
"title to be announced"
Dr. Dr. Simone Kreth, "Prognostic Impact
of Gene Expression Analyses in Human Glioblastoma"
Dr. Gudrun
Tellmann, "High-Throughput
Gene Expression Analysis Using the LightCycler Platform"
HRM
–
High Resolution Melting - Epigentics
SNP analysis, HRM = high resolution melt applications, Epigenetics,
methylation markers, HRM platform comparison, etc …
Prof.
Carl Wittwer, "High Resolution Melting Analysis"
Prof. Claudio Orlando,
“High Resolution Melting Analysis in Cancer
Diagnosis”
CNA
- Circulating Nucleic Acids
Analysis of circulating RNAs and DNA and microRNAs as diagnostic and
prognostic marker, …
Dr.
Pamela Pinzani, “Cell
free circulating DNA”
Dr.
Alfred Schöller, "Targeting the human urine RNAome for
tumor diagnostics by qPCR”
Single-cell
qPCR
Single-cell sampling, pre-amplification techniques, laser
microdissection, sub-cellular PCR, micro-manipulation of cell clusters,
cellular micro injection, FACS spotting, single cell handling,
pre-amplification…
Dr.
Michael W. Pfaffl,
“Quantitative expression analysis after
pre-amp in single WBCs”
Dr.
Anders Stahlberg,
“Single-cell gene expression profiling”
RNAi
-
microRNA - siRNA
Applications – miRNA normalisation
RNAi mechanism, microRNA extraction, qRT-PCR technologies to detect
microRNA, microRNA normalisation strategies, siRNA applications in
combination with qRT-PCR, microRNA targets and microRNA precursors, new
siRNA manipulation and microRNA technologies, ...
Prof.
Jo Vandesompele,
“MicroRNA and mRNA gene expression
normalization”
qPCR
BioStatistics &
BioInformatics
software applications, data mining, calculation of relative expression,
primer and probe design on mRNA and microRNA level, real-time PCR
efficiency determination, mathematical modelling, multivariate
expression profiling, statistics in real-time PCR, data management,
multiway expression profiling, multiple regression analysis, 3D
data visualization, ...
Dr.
Ales Tichopad,
“Statistical aspects of quantitative PCR
experiment design and qPCR data analysis”
Dr.
Jan Hellemans,
“Accurate and objective copy number profiling
using real-time quantitative PCR”
Dr.
Anders Bergkvist,
“Expression profiling - clusters of
possibilities”
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Quantitative PCR
techniques
Course Date: 14-16 July 2010, Breda (NL)
http://www.expertiseteam-separationsciences.nl/avans-training-2548.htm
Aim of the course:
Quantitative Polymerase Chain Reaction is becoming more and more
important in almost all laboratories. The aim of this international
short-course is to give the participants an in-depth view in the
theoretical and practical knowledge of Quantitative PCR techniques.
State-of-the-art experts in the field of Quantitative PCR techniques
from several research and diagnostic laboratories will provide further
theoretical and practical expertise.
Participants:
This course is developed for experienced laboratory technicians or
researchers involved in the life sciences laboratories, incl. medical
diagnostics, microbiology, genetics, pathology, immunology,
agriculture, biochemistry, etc.
Basic knowledge of molecular biology in general and PCR is a
prerequisite. If you feel you lack the basic knowledge in PCR or the
principles of real-time PCR, but you would like to participate in this
quantitative PCR course, please note that the Avans Hogeschool also
offers an international introductory short course: Principles,
parameters, and applications of PCR (code 2545), which is especially
suitable as a basic introduction to this course.
Course structure:
The course consists of a series of practical sessions interspersed with
lectures and tutorials. 50% of the time is devoted to lectures and 50%
is spent on "hands-on" experiments, covering techniques that may be
directly applied in research and diagnostic laboratories. All
participants will receive a practical manual containing all used
protocols and a reference manual "Rapid cycle real-time PCR", Springer
Verlag, Berlin.
Course content:
* Day 1: Pre-qPCR: Sample preparation and RNA
isolation for qPCR
* Day 2: qPCR: Quantitative PCR techniques
(including probelibrary)
* Day 3: Post-qPCR: Normalisation and data analysis
Theory:
* RNA isolation and conventional quantitative PCR
techniques
* Real-time quantitative PCR and mathematics
* Trouble shooting and discussion of the qPCR
problems
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qPCR
Seminars and Education Programs:
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Free
of charge web
seminars
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
Expert
speakers
will cover areas such as:
- QPCR assay design and
optimisation
- Template handling and
purification
- Focus on the
optimisation of RT-qPCR
- Various approaches to
genetic analysis
- Working with limiting
samples
- Data analysis
- Troubleshooting
- Assay improvement
option for challenging assays
- LNA technology and
application
Seminar
grading key:
- Level 1 - terrified
and beginners
- Level 2 - something
for everyone but a background knowledge is assumed
- Level 3 - improvers,
seminars focused on specific areas of interest
- Level 4 - brave and
experienced, delving into the fine detail and the more troublesome
aspects of the technique
| Available
Seminars: |
| Author |
Seminar
Title |
| Mudassir Mohammed, Ph.D. |
An Introduction to qPCR |
| Valerie Hawkes, Ph.D. |
Assay Design and
Optimisation |
| Neven Zoric, M.Sc. |
Approaches to Normal qPCR —
Facing the Challenge
of Normalization |
| Tania Nolan, Ph.D. |
Deriving a Troubleshooting
Protocol |
| Mikael Kubista, Prof. |
A Statistical Approach to
qPCR Gene Expression
Data Analysis |
| Marina Tshuikina, Ph.D. |
An Introduction to
Epigenetics Analysis by qPCR |
| Valerie Hawkes, Ph.D. |
Improving the
Discrimination and Sensitivity of
qPCR Assays using LNA |
| Chris Bass, Ph.D. |
Development of DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito Disease Vector Populations |
| Michael Pfaffl, Ph.D. |
Influence of RNA Integrity
on Real-Time RT-PCR
Quantification Data |
| Vladimir Benes |
microRNA Profiling |
| Rebecca Hands |
Technical Tips on LCM
Extraction for mRNA
Profiling |
| Jens Stolte |
cDNA Amplification with
GenomePlex® Complete
Whole Genome Amplification Kit |
| Tanya Novak, M.Sc. |
The SPUD Assay Has an
Important Role in the
Interpretation of Detecting Pneumocystis DNA in Clinical Specimens |
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Stratagene
- Fast Track
qPCR Education Program
http://www.stratagene.com/tradeshows/fasttrack.aspx |
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The Fast Track QPCR
Education Program is a comprehensive program of user training and
advanced education for users of our real-time PCR instruments. The
program provides a wide range of tools to get you up and running with
real-time PCR quickly and effectively. The program also offers you
access to expert Field Application Scientists providing advanced
education. As a leader in the training of scientists on real-time PCR,
we are proud to offer the Fast Track Education Program.
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Fast
Track QPCR Education
Program Benefits:
- Rapid
and effective QPCR training
- Advanced
application-focused education
- On-demand
educational tools
- Ongoing
education to
support new personal and evolving assays
- Consultation
with expert Field Application Scientists
Fast
Track QPCR Education
Program:
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Stratagene
Online Seminars
http://www.stratagene.com/onlineseminars/ |
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Stratagene
offers a variety of live and recorded online seminars
presented by one of our Technical Services Scientists. Simply select a
category of interest below to view the abstracts and to register for a
presentation.
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Technical
Training Courses
http://www.appliedbiosystems.com/service/training/
https://learn.appliedbiosystems.com/
https://learn.appliedbiosystems.com/realtimepcr
https://learn.appliedbiosystems.com/pcr
Basic Real-Time PCR
training EU
(2 days)
Objective:
In this course, the participant is trained on the basic knowledge of
Real-Time PCR and its applications for quantitative and SNP analysis.
With the use of practical examples, this course focuses on the correct
use of the web-based tools for searching genomic TaqMan. assays and the
SDS and the Primer Express. software. In addition, an overview of the
diverse applications of Real-Time PCR is presented.
This
training course is optimized for scientists new to ABI PRISM. Real-Time
PCR platforms and who wish to establish and optimally use the system
for Real-Time PCR and SNP analysis applications.
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