Available real-time PCR cyclers (3)
Available real-time PCR cyclers (1)
Available real-time PCR cyclers (2)
Available real-time PCR cyclers (4)



banner MIC qPCR

Mic is a small 48-well qPCR instrument that uses magnetic induction technology for rapid real time cycling. Available with either 2 or 4 channels, the advantages of the Mic include:

•    Speed – complete high quality qPCR runs in less than 25 min.
•    Accuracy – excellent repeatability and reproducibility with a temperature uniformity of ±0.05°C.
•    Size – At 2 kg the Mic takes up less space on a bench than a lab book.
•    Connectivity – Bluetooth® option allows up to 10 instruments to run from one PC.
•    Plug and play with no calibration required – ever!

Mic’s user friendly software is packed full of intelligent analysis features including:

•    Cycling analysis - with LinRegPCR option (Ruijter et al. 2009)
•    Melt analysis - including genotyping with dual hybridisation probes
•    Absolute Quantification - including Standard Curve
•    Relative Quantification - incorporating the REST algorithm (Pfaffl et al. 2002)
•    Allelic Discrimination
•    High Resolution Melting

With an intuitive software layout, run setup and analysis become simple and there are many quality control features to provide confidence in the final result. The software has been designed to meet the MIQE guidelines.
Discover more at www.mic-qpcr.com


Qiagen - Rotor-Gene Q with HRM



Real-time PCR detection  -  Rotor-Gene Q - pure detection
QIAGEN’s new real-time PCR cycler, the Rotor-Gene Q, provides outstanding performance and the reliable and accurate results your research demands. Together with optimized QIAGEN kits, the Rotor-Gene Q enables streamlined analysis for a wide range of applications.
    * Outstanding thermal and optical performance
    * More applications than any other real-time cycler
    * Unmatched optical range with up to 6 channels
    * Robust design enables easy assay setup and minimal maintenance
    * Compatible with the QIAgility for automated PCR setup

The unique rotary design delivers:
    * Well-to-well variations below ±0.01°C (20 times lower than block cyclers)
    * Uniform detection eliminating the need for ROX reference dye
    * Fast ramping and negligible equilibration times for short run times
    * Complete confidence in your results!

Comprehensive application range
The Rotor-Gene Q combined with optimized QIAGEN real-time PCR kits and assays offer reliable results in all real-time PCR and high-resolution melt applications, including:
    * Gene expression analysis
    * Pathogen detection
    * DNA methylation analysis
    * Genotyping
    * Mutation scanning
    * High Resolution Melting analysis

Rotor-Gene ScreenClust HRM Software for highly effective high-resolution melting analysis
    * Innovative mathematical approach to HRM analysis
    * Highly accurate identification of genotypes in supervised mode
    * Automatic detection of new mutations in unsupervised mode
    * Robust statistics for classifying and interpreting HRM data
    * Minimal effort and standardized processes for data interpretation

HRM - an innovative technology for analysis of genetic variations
View and listen to this seminar





Principle and procedure - Unique rotary design for outstanding performance

The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure "Cross-section of the Rotor-Gene Q"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure "Precise real-time PCR analysis"). It also eliminates sample-to-sample variations and edge effects. These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways.
The rotary design delivers:

    * Well-to-well variation below ±0.01°C (20 times less than block cyclers)
    * Uniform detection eliminating the need for ROX reference dye
    * Fast ramping and negligible equilibration times for short run times
    * Complete confidence in your results!

Unrivaled optical range enables multiple applications

Whether your assay is based on intercalating dyes such as SYBR Green, probes such as hydrolysis (TaqMan) or hybridization (FRET) probes, or multiplex chemistries, the Rotor-Gene Q meets your requirements. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see Table "Channels for optical detection"). In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future.




REST 2009 Software
For gene expression analysis using real-time PCR data from the Rotor-Gene Q and other cyclers

    * Estimates up and down regulation for gene expression studies
    * Analysis using randomization and bootstrapping techniques
    * Graphical data output via whisker-box plots

REST 2009 Software is a standalone software tool, developed by M. Pfaffl (Technical University Munich) and QIAGEN, for analysis of gene expression data from quantitative real-time PCR experiments. Click the "Resources" tab to download REST 2009 Software free of charge.
Download newest version REST 2009  =>  http://www.REST.de.com
Download User Guide  => 
REST 2009 Software User Guide - English (PDF)





The Rotor-Gene 6000 is a third-generation real-time rotary analyzer. Its unique centrifugal format was designed specifically for the demanding requirements of real-time cycling and thermo-optical analyses.

The rotary design provides unparalleled well-to-well thermal and optical uniformity—critical factors in real-time analysis. Its also enables a fast data acquisition rate, a feature now proving to be essential for advanced applications such as HRM (high-resolution melt).

In short, the superior performance of the Rotor-Gene 6000 delivers versatility:
  • It is the only real-time amplification platform capable of HRM (high-resolution melt) and nucleic acid concentration measurement in addition to all the standard real-time applications like quantitative amplification and SNP genotyping.
  • It has the greatest optical range, with 6 channels spanning UV to infra-red wavelengths.
  • It uses the widest range of interchangeable reaction tube formats.
With the Rotor-Gene 6000 you can embrace or invent the future of real-time analysis and unlock new research possibilities.   MORE…




SmartCycler II & GeneXpert (Cepheid)

  

The "heart" of the Smart Cycler® System from Cepheid of Sunnyvale, Calif., is the I-CORE™ (Intelligent Cooling/Heating Optical Reaction) module. According to company literature, the I-CORE module incorporates state-of-the-art microfluidic and microelectronic design. Each Smart Cycler processing block contains 16 independently programmable I-CORE modules, each of which performs four-color, real-time fluorometric detection. A wide variety of different multiplex or simplex fluorescent tags can used in conjunction with this system, including  FAM, TET, ALEXA 532, ALEXA 647, TAM, ROX, SYBR Green, Cy3, Texas Red and others.

Main features of the SmartCycler II system:

•     Expandable: 16, 32, 48, 64, 80 or 96 independent reaction sites
•     Flexible
•     Fast
•     Real-time detection
•     Four-channel optics

The GeneXpert® System utilizes real-time polymerase chain reaction (PCR) to amplify and detect target DNA. The system is the first to fully automate and integrate all the steps required for PCR-based DNA testing: sample preparation, DNA amplification and detection.

Self Contained Cartridges

Integrated sample preparation - for automated and self-contained testing, features and benefits:

  • Reagent containment and delivery
  • Sample and reagent aliquoting and mixing; continuous flow for large volume handling
  • Cell separation and concentration using modular filter and solid-phase holder assemblies
  • Rapid cell lysis using ultrasonic techniques
  • DNA or RNA capture, enrichment, purification using chips or solid-phase materials
  • Preparing reaction mixture and filling integrated PCR reaction tube
  • The cartridges are designed for use with Cepheid's real-time modules, thus fully integrating the steps associated with DNA extraction, amplification, and detection in one system and one procedure.


mx family image

The Mx™ Family of qPCR Systems from Stratagene offers the highest performance and flexibility in qPCR Systems at the most affordable price. The Mx3000P® qPCR System is not just an entry-level QPCR system, but is a high-performance, full-featured instrument system designed to accommodate most experimental designs. The new Mx3005P™ qPCR System advances the proven Mx3000P system by offering unmatched flexibility and capability to support more real-time qPCR applications and chemistries. The flexible Mx3005P system will accommodate your research needs now and in the future.


Mx3000P® qPCR System
Cited in more than 3000 publications, the Agilent Mx3005P and Mx3000P QPCR Systems are the most flexible—and reliable—instruments available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Agilent’s qPCR software, MxPro, provides users with an intuitive interface, quick experiment design, powerful data analysis and easy report generation. All of these features and more make qPCR with the Mx instrument an exciting and dependable user experience.

Mx3005P™ qPCR System
Cited in more than 3000 publications, the Agilent Mx3005P and Mx3000P QPCR Systems are the most flexible—and reliable—instruments available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Agilent’s qPCR software, MxPro, provides users with an intuitive interface, quick experiment design, powerful data analysis and easy report generation. All of these features and more make qPCR with the Mx instrument an exciting and dependable user experience.

MXPro ET QPCR Software
Mx Pro QPCR software with FDA guideline 21 CFR Part 11 compatible features. Four key elements have been added to create the ET software version; secure application login, database file management, electronic audit trail, and report generation.


Mx3000P® and Mx3005P™ qPCR System Features

Instrument Features:

  • Excitation: Quartz tungsten-halogen lamp via scanning fiberoptics
  • Detector: Single photomultiplier tube (PMT) via scanning fiberoptics
  • Filters: Customizable filter wheels with four (Mx3000P® system) or five (Mx3005P™ system) user-selected filter sets
  • Filter control: Custom filter path selection to support FRET signal detection (Mx3005P™ system only)
  • Thermal system: Solid state, Peltier-based, 96-well block thermal system
  • Sample format: Standard 96-well plates, 8-strip tubes, 200-µl tubes, optimized for 25-µl reactions
  • Embedded computer collects and saves experiment data during power failure or computer crash
  • Dimensions: 13" (33 cm) W x 18" (46 cm) D x 17" (43 cm) H, Weight: 45 lbs. (20.4 kg)
  • Power: 100 to 230 VAC +/- 10%, 50/60 Hz
System Performance:
  • Linear dynamic range: 10 orders of magnitude, standard curve method
  • Excitation range: 350 to 750 nm
  • Emission range: 350 to 700 nm
  • Temperature uniformity: +/- 0.25°C at 72°C
  • Thermal block ramp rate: Up to 2.5°C/second
  • Amplification rate: Standard 40-cycle, two-step QPCR reaction completed in 90 minutes; reaction is completed in 60 minutes using FullVelocity™ SYBR® Green QPCR reagents (without dissociation curve)
  • Discrimination: Distinguish samples with 5,000 and 10,000 template copies with a 99.7% confidence level
  • Detection down to single-copy equivalents
MXPro ET QPCR Software
  • Automatically detects and interfaces with Mx3000P (4-color) or Mx3005P system (5-color) system
  • Interfaces with Mx3000P and Mx3005P systems simultaneously and supports up to six systems in any combination attached to a single computer
  • Streamlined plate setup with dye editing dropdown menu
  • Right mouse-click capability throughout the software to allow quicker and easier access to menu functions
  • The Comparative Quantification module generates and displays normalized fold change values on a chart using a base 2 logarithmic scale.
  • Gene expression fold change is displayed with upper and lower limit error bars based on replicate variability for up to four targets normalized to a single reference gene with efficiency corrections for each gene target
  • View amplification plots and standard curves for up to five targets on the same screen with threshold changes instantly updated on the standard curve
  • Improved text reports display target-specific data analysis parameters at the top of each report and allow for customization through adding/removing columns and enhanced sorting capability
  • Export any data set directly into .xls, .ppt, .bmp, .txt, or .xml format. Charts and graphs can be exported directly to Microsoft® PowerPoint® and Excel®
  • Auto generation of charts and graphs in Excel during export
  • Create custom consolidated data reports by selecting which analysis screens to include and how the report will organize the data
Stratagene's Mx™ family of qPCR Systems, premiere qPCR System Service Program, complete line of qPCR and qRT-PCR reagents, and Fast Track qPCR Education Program provides a total package for your qPCR research.



Sacace SaCycler 96







Sacace - SyCycler 96 - A new instrument for real-time amplification and melting analysis suitable both for research studies as well as for diagnostics applications. Two thermoeletric Peltier elements ensure high accuracy of temperature regulation and noiselessness.
The unique design and project of the thermal block allows loading and unloading of test samples in a completely automated software-controlled way.

  • 4 or 5 channels multiplexing for discrimination of up to five targets in a single reaction well*
  • Strong flexibility thanks to the 96-well format suited for standard PCR microplates, test tubes and strips
  • Possibility to use tubes with different heights with regulation of the matrix block
  • Optimal signal/noise ratio and absence of crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera
  • Light emitting diodes (LED) as a light source with a lifetime of about 100,000 hours that does not require maintenance or constant
  • Wide dynamic range of detection using multiple exposure method, which leads the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for fluorescence settings
  • Main applications are Real-Time quantitation, single nucleotide polymorphisms (SNPs) genotyping, melting curve and gene expression analysis
  • Instrument comes with the most used Sacace protocols already installed in the software minimizing possibility of error during programming.

SaCycler96

SaCycler - 96 download technical characteristics












TAKARA TP800 -  Thermal Cycler Dice™ Real Time System

A real-time PCR that offers an accurate and reproducible quantification is indispensable for gene expression analysis.
Takara Bio launched a real-time PCR instrument for 96 plate. Thermal Cycler Dice™ Real Time is a compact system for 96 plate type with high-performance hardware and easy-to-use software. We recommend this system as a "Perfect" real-time PCR instrument with confidence.

Download brochure here

This product is not available in the U.S. and Europe.