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The focus of this newsletter issue
Join the new eConferences streaming webportal
-- Amplify your knowledge!
This portal is dedicated to
scientists from the community of qPCR, digital-PCR, NGS and Molecular
Diagnostics. You’ll find here all the records from around 200
presentations held at qPCR & NGS Events in the past years. We
provide the presentations via movie streaming technology in high
quality - high resolution and perfect sound quality in high speed - on
any internet browser or mobile device, including Android or iOS.
Microvesicles and Exosomes in Molecular Diagnostics
cell-derived vesicles that are present in many tissues and (perhaps) in
all biological fluids, including blood, milk, urine, sweat and cell
culture supernatant. The reported size of exosomes is between 30 and
100 nm in diameter. It is becoming increasingly clear that exosomes
have specialized functions and play a key role in
inter-cellular communication, e.g. in the immune system, in
cancer progression, in coagulation, in intercellular signaling, and in
cellular waste management.
there is a growing interest in molecular diagnostic and in
clinical application of exosomes. Exosomes can potentially be
used for prognosis, therapy, and biomarkers for health or disease,
especially in cancer progression and metastasis.
A lot of research is done in exosome purification and
isolation, their size and content characterization, by
quantifying surface- and intra-luminal proteins, membrane fatty acids,
and the high concentrated regulative small RNA.
|Optimising the mircrovesicle and exosome isolation
Standardization of sample collection, isolation and
analysis methods in extracellular vesicle research.
Witwer KW, Buzás EI, Bemis LT, Bora A, Lässer C, Lötvall J, Nolte-'t
Hoen EN, Piper MG, Sivaraman S, Skog J, Théry C, Wauben MH, Hochberg F.
J Extracell Vesicles. 2013 May 27;2
The impact of disparate isolation methods for
extracellular vesicles on downstream RNA profiling.
Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K,
Vandesompele J, Bracke M, De Wever O, Hendrix A.
J Extracell Vesicles. 2014 Sep 18;3 -- eCollection 2014.
Methods of isolating extracellular vesicles impact
down-stream analyses of their cargoes.
Douglas D. Taylor & Sahil Shah
Comparison of ultracentrifugation, density gradient
separation, and immunoaffinity capture methods for isolating human
colon cancer cell line LIM1863-derived exosomes.
Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM,
Methods. 2012 Feb;56(2): 293-304
Possibilities and limitations of current technologies
for quantification of biological extracellular vesicles and synthetic
Maas SL, de Vrij J, van der Vlist EJ, Geragousian B, van Bloois L,
Mastrobattista E, Schiffelers RM, Wauben MH, Broekman ML, Nolte-'t Hoen
J Control Release. 2014 200C: 87-96
Single-step isolation of extracellular vesicles by
Böing AN, van der Pol E, Grootemaat AE, Coumans FA, Sturk A, Nieuwland R
J Extracell Vesicles. 2014 8;3 -- eCollection 2014.
Current methods for the isolation of extracellular
Momen-Heravi F, Balaj L, Alian S, Mantel PY, Halleck AE, Trachtenberg
AJ, Soria CE, Oquin S, Bonebreak CM, Saracoglu E, Skog J, Kuo WP.
Biol Chem. 2013 Oct;394(10): 1253-1262
Methods for extracellular vesicles isolation in a
Sáenz-Cuesta M, Arbelaiz A, Oregi A, Irizar H, Osorio-Querejeta I,
Muñoz-Culla M, Banales JM, Falcón-Pérez JM, Olascoaga J, Otaegui D.
Front Immunol. 2015 Feb 13;6: 50
... ... more papers here Exosomes.Gene-Quantification.info
|Exosome Function and Physiology
Microvesicles as mediators of intercellular
communication in cancer -- the emerging science of cellular 'debris'.
Lee TH, D'Asti E, Magnus N, Al-Nedawi K, Meehan B, Rak J.
Semin Immunopathol. 2011 Sep;33(5): 455-467
Secreted microRNAs -- a new form of intercellular
Chen X, Liang H, Zhang J, Zen K, Zhang CY.
Trends Cell Biol. 2012 22(3):125-32
Extracellular vesicle-depleted fetal bovine and human
sera have reduced capacity to support cell growth.
Eitan E, Zhang S, Witwer KW, Mattson MP
J Extracell Vesicles. 2015 Mar 26;4: 26373 -- eCollection 2015
Extracellular vesicles -- potential roles in
De Jong OG, Van Balkom BW, Schiffelers RM, Bouten CV, Verhaar MC
Front Immunol. 2014 Dec 3;5: 608
Therapeutic potential of extracellular vesicles.
Merino AM, Hoogduijn MJ, Borras FE, Franquesa M
Front Immunol. 2014 Dec 19;5: 658
The Trojan exosome hypothesis.
Gould SJ, Booth AM, Hildreth JE.
Proc Natl Acad Sci U S A. 2003 100(19): 10592-10297
... ... more papers here Exosomes.Gene-Quantification.info
Journal -- "Biomolecular Detection and Quantification" -- Call
Detection and Quantification (BDQ) is an open access, peer-reviewed
international journal dedicated to championing excellence in molecular
study design, measurement, data analysis and reporting. Its focus is on the application of
qualitative and quantitative molecular methodologies to all areas of
clinical and life sciences. Download the recent BDQ papers on Elsevier Science
The journal has two main
- to provide a forum for
discussion and recommendation of guidelines designed to improve the
accuracy of molecular measurement, its data analysis and the
transparency of its subsequent reporting;
- to publish molecular biology
based studies that adhere to best practice guidelines, both current and
Benefits of publishing
open access with Elsevier's Biomolecular Detection and
Quantification - www.journals.elsevier.com/biomolecular-detection-and-quantification/
- Peer-reviewed journal
indexed by Scopus and supporting ORCID and Crossref to help maintain
your publication record.
- A renowned and
respected editorial team with extensive knowledge of your
research field. BDQ was established by a group of scientists based on
their experience developing and publishing the Minimum Information for
Publication of Quantitative Real-Time (and Digital) PCR Experiments
(the MIQE and digital MIQE guidelines).
- Reaching key audiences
with 10 million active users per month using our publishing platform
- Choice of user licenses
so authors can decide how best to publish open access.
- A discount on
the publication fee for a limited time.
For more information about
publishing Open Access with Elsevier, including funding body
arrangements, institutional agreements and more, visit: www.elsevier.com/openaccess
We look forward to receiving your
Stephen Bustin, Jim
Huggett, Justin O'Grady, Michael W. Pfaffl, Carl Wittwer, Ron Cook
View full editorial board
real-time PCR data analysis software
GenEx 6 available - The most powerful tool
for complex qPCR data analysis
GenEx is a popular software for qPCR data processing and analysis. Built
in a modular fashion GenEx provides a multitude of functionalities for
the qPCR community, ranging from basic data editing and management to
advanced cutting-edge data analysis. Download GenEx User Guide
Biocenter, Europe´s leading provider of genomic services using
quantitative real-time PCR (qPCR), and MultiD Analyses, Europe’s prime
software developer for the analysis of multivariate data, release GenEx
version 6 for accurate analysis of qPCR data compliant with current
Clinical Laboratory Standards Institute (CLSI) guidelines.
new analyses in GenEx 6 include: Estimating PCR efficiency, testing
for outliers, testing linear model, estimating dynamic range,
estimating random error, estimating limit of detection, estimating
limit of quantification, estimating concentrations of unknowns,
evaluation of precision, and verification of precision. For all
estimates confidence intervals are calculated.
Additional new features
include: Transcript distribution for single cell analysis, Survival
Analysis, Receiver Operator Characteristics (ROC), Wizard for ProSeek
protein analysis, and reader for 3D Gene microRNA analysis.
our GenEx webpage and download a FREE GenEx 6 trial version =>
Join all GenEx
talks by Prof. Mikael Kubista held at recent qPCR & NGS Events
Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
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