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Gene Quantification Newsletter
May & June 2017

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GenEx 6.1 offers advanced methods to analyze real-time qPCR data with simple mouse clicks

GenEx is a popular software for qPCR data processing and analysis. Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management to advanced cutting-edge data analysis.

Basic data editing and management
Arguably the most important part of qPCR experiments is to pre-process the raw data into shape for subsequent statistical analyses. The pre-processing steps need to be performed consistently in correct order and with confidence. GenEx Standard’s streamlined and user-friendly interface ensures mistake-free data handling. Intuitive and powerful presentation tools allow professional illustrations of even the most complex experimental designs.

Advanced cutting-edge data analysis
When you need more advanced analyses GenEx Enterprise is the product for you. Powerful enough to demonstrate feasibility it often proves sufficient for most users demands. Current features include parametric and non-parametric statistical tests, Hierarchical Clustering Analysis (HCA), Heatmaps, Principal Component Analysis (PCA), and Artificial Neural Networks. New features are continuously added to GenEx with close attention to customers’ needs.

New features
Sample handling and samples individual biology often contribute to confounding experimental variability. By using the new nested ANOVA feature in GenEx version 6.1 user will be able to evaluate variance contributions from each step in the experimental procedure. With a good knowledge of the variance contributions, an appropriate distribution of experimental replicates can be selected to minimize confounding variance and maximize the power of the experimental design! For experiments with complex features, such as for example multifactorial diseases, analytical relationships and classifications may not readily be available. The support vector machine feature in the new version of GenEx is so easy to use that it will make this advanced supervised classification method easily available to novice users, while providing access to advanced parameters for experts.

View our webpage and download a FREE GenEx 6.1 trial version =>   GenEx.Gene-Quantification.info


GenEx 6.1 -- the software is compliant with MIQE and CLSI guidelines for the analysis of real-time PCR data

TATAA Biocenter, Europe´s leading provider of genomic services using quantitative real-time PCR (qPCR), and MultiD Analyses, Europe’s prime software developer for the analysis of multivariate data, release GenEx version 6 for accurate analysis of qPCR data compliant with current MIQE guidelines and Clinical Laboratory Standards Institute (CLSI) guidelines.

The new analyses in GenEx 6.1 include:   Estimating PCR efficiency, testing for outliers, testing linear model, estimating dynamic range, estimating random error, estimating limit of detection, estimating limit of quantification, estimating concentrations of unknowns, evaluation of precision, and verification of precision. For all estimates confidence intervals are calculated.

Additional new features include:   Transcript distribution for single cell analysis, Survival Analysis, Receiver Operator Characteristics (ROC), Wizard for ProSeek protein analysis, and reader for 3D Gene microRNA analysis.

“I have been using GenEx for all my qPCR analyses for the past 5 years. With GenEx I can be sure that my data normalization and analyses will be MIQE compliant. Whether I’m analyzing qPCR arrays, data with a standard curve or determining a relative difference, it takes little time to import the files I need and obtain my final data set”
says Greg Shipley, President of Shipley Consulting, LLC.

“We have been using GenEx for many years at Technical University of Munich. With every new released version we are impressed by the many clever functionalities introduced for RT-qPCR analysis, especially the data normalisation and visualisation tools like PCA or HCA”, says Michael W. Pfaffl. “The new CLSI compliant analyses are most interesting to us and a natural extension of the MIQE guidelines”.



Listen to GenEx talks by Prof. Mikael Kubista

View our webpage and download a FREE GenEx 6.1 trial version => GenEx.Gene-Quantification.info

 
GenEx result screenshots:
   

 Heatmap -- Hierarchical Clustering Analysis (HCA)

                      Vulcano Plot

   

3D PCA  (Principal Component Analysis)

             Variance Analysis via Nested ANOVA
 
   

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