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Gene Quantification Newsletter
September 2015

is sponsored by


Streamlined and automated NGS workflow

  Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on www.Gene-Quantification.info
The focus of this qPCR NEWS issue is:


Streaming portal

Join the updated
eConferences streaming webportal
www.eConferences.de -- Amplify your knowledge in  qPCR, dPCR and NGS !
This streaming portal is dedicated to scientists from the community of qPCR, digital PCR, Next Generation Sequencing (NGS), MicroGenomics (MG) and Molecular Diagnostics (MDx). You’ll find here all the records of 280 presentations held at qPCR & NGS and MG Events in the past years – qPCR 2010 in Vienna to qPCR & NGS 2015 in Freising-Weihenstephan.
We provide the presentations via movie streaming technology in high quality – high resolution and perfect sound quality in high speed – on any internet browser or mobile device.

qPCR Data Analysis and BioInformatics

Molecular Regulatory Networks
Gene regulatory networks explicitly represent the causality of developmental processes. They explain exactly how genomic sequence encodes the regulation of expression of the sets of genes that progressively generate developmental patterns and execute the construction of multiple states of differentiation.

Gene Regulatory Networks.
Eric Davidson and Michael Levin
PNAS vol. 102 no. 14

Gene regulatory networks for development.
Michael Levine and Eric H. Davidson
PNAS (2005) 102 (14): 4936–4942

Inferring gene expression regulatory networks from high-throughput measurements.
Zavolan M
Methods. 2015 Sep 1;85: 1-2

ARMADA -- Using motif activity dynamics to infer gene regulatory networks from gene expression data.
Pemberton-Ross PJ, Pachkov M, van Nimwegen E
Methods. 2015 Sep 1;85: 62-74

Posttranscriptional Regulatory Networks:  From Expression Profi ling to Integrative Analysis of mRNA and MicroRNA Data.
Swanhild U. Meyer, Katharina Stoecker, Steffen Sass, Fabian J. Theis and Michael W. Pfaffl
Chapter 15  in  Quantitative Real-Time PCR: Methods and Protocols   (Methods in Molecular Biology) by Roberto Biassoni, Alessandro Raso

Toward understanding the evolution of vertebrate gene regulatory networks: comparative genomics and epigenomic approaches.
Martinez-Morales JR
Brief Funct Genomics. 2015 Aug 20.

Visualization of omics data for systems biology.
Gehlenborg N, O'Donoghue SI, Baliga NS, Goesmann A, Hibbs MA, Kitano H, Kohlbacher O, Neuweger H, Schneider R, Tenenbaum D, Gavin AC.
Nat Methods. 2010 7(3 Suppl): S56-68

ESCHER -- A Web Application for Building, Sharing, and Embedding Data-Rich Visualizations of Biological Pathways.
King ZA, Dräger A, Ebrahim A, Sonnenschein N, Lewis NE, Palsson BO
PLoS Comput Biol. 2015 Aug 27;11(8): e1004321 -- eCollection 2015

.. and more Molecular Regulatory Networks -- Bioinformatics.Gene-Quantification.info



MIQE & qPCR iBook
How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide
1st edition published in May 2015
Editors:  Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download via iTunes --

Throughout the past 30 years Polymerase Chain Reaction (PCR) has proven to be the most powerful technique in a scientist toolbox. Only few techniques had a comparable success story like PCR. This MIQE & qPCR iBook will guide you in better practicing in your laboratory thanks to the MIQE guideline. More info here!

Please let us know, if you want to participate with an own application or chapter in the MIQE & qPCR iBook
Contact us via  iBook@bioMCC.com

MIQE & qPCR iBook – a digital publication: How making the MIQE guidelines easier to follow.
Afif M. Abdel Nour & Michael W. Pfaffl
Presentation at qPCR & NGS 2015 Symposium --


GenEx 6 available:
The most powerful tool for complex qPCR data analysis 

Download a FREE GenEx 6 trial version => GenEx.Gene-Quantification.info
GenEx is a popular software for qPCR data processing and analysis. 
Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management to advanced cutting-edge data analysis. Download GenEx User Guide


Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages



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