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Gene Quantification Newsletter
May 2015

is sponsored by

     
     

Streamlined and automated NGS workflow


 
  Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), are compiled and summarised on the www.Gene-Quantification.info
The focus of this newsletter issue is:
   


 

                                                                     

Streaming portal

Join the new eConferences streaming webportal -- www.eConferences.de -- Amplify your knowledge!
This portal is dedicated to scientists from the community of qPCR, digital-PCR, NGS and Molecular Diagnostics. You’ll find here all the records from around 200 presentations held at qPCR & NGS Events in the past years. We provide the presentations via movie streaming technology in high quality - high resolution and perfect sound quality in high speed - on any internet browser or mobile device, including Android or iOS.

 

 

MIQE & qPCR iBook
How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

1st edition published 11th May 2015
Editors:  Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download via iTunes --
https://itunes.apple.com/de/book/miqe-qpcr/id993276375?mt=11

Throughout the past 30 years Polymerase Chain Reaction (PCR) has proven to be the most powerful technique in a scientist toolbox. Only few techniques had a comparable success story like PCR. This iBook will guide you in better practicing in your laboratory thanks to the MIQE guideline.

Please let us know, if you want to participate with an own application or chapter in the MIQE & qPCR iBook
Contact us via  iBook@bioMCC.com

 
iBook chapters and authors:

 
  • Preface
  • Introduction & The history of MIQE
  • Quantification strategies
  • RNA quality control
  • qPCR oligonucleotides / Primer design software
  • qPCR instrumentation
  • Selection of reference genes
  • Data analysis & statistics
  • MIQE compliant qPCR workflow control
  • MIQE guidelines and microRNA
  • Digital PCR Applications and Assays
  • MIQE compliance using qPCR arrays
  • MIQE translations & online applications
  • Credits & Imprint
  • Afif Abdel Nour & Michael W. Pfaffl
  • Stephen Bustin et al.
  • MW Pfaffl
  • Eva Schmidt et al.
  • James Flynn et al.
  • Nick Newton et al.
  • Philip Zimmermann et al.
  • Mikael Kubista, Amin Forootan et al.
  • M Hren, K Zupancic, T Berto et al.
  • Ditte Andreasen, et al.
  • Yann Jouvenot & Viresh Patel
  • Greg Shipley
  • Afif Abdel Nour
  • Afif Abdel Nour & Michael W. Pfaffl
   
 
     

 

 

 

 

 

 

 
MIQE & qPCR iBook – a digital publication: How making the MIQE guidelines easier to follow
Afif M. Abdel Nour & Michael W. Pfaffl
Presentation at qPCR & NGS 2015 Symposium --
www.eConferences.de/MIQE-qPCR-iBook/
 
... ... more screenshots and info about the MIQE & qPCR iBook at 
qPCRbooks.Gene-Quantification.info
 

 

20th World Congress on
Advances in Oncology

&

18th Intern. Symposium
on
Molecular Medicine


 

GenEx 6:
real-time PCR data analysis software
 

GenEx 6 available - The most powerful tool for complex qPCR data analysis
GenEx is a popular software for qPCR data processing and analysis. 
Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management to advanced cutting-edge data analysis. Download GenEx User Guide

TATAA Biocenter, Europe´s leading provider of genomic services using quantitative real-time PCR (qPCR), and MultiD Analyses, Europe’s prime software developer for the analysis of multivariate data, release GenEx version 6 for accurate analysis of qPCR data compliant with current Clinical Laboratory Standards Institute (CLSI) guidelines.

The new analyses in GenEx 6 include: Estimating PCR efficiency, testing for outliers, testing linear model, estimating dynamic range, estimating random error, estimating limit of detection, estimating limit of quantification, estimating concentrations of unknowns, evaluation of precision, and verification of precision. For all estimates confidence intervals are calculated.
Additional new features include: Transcript distribution for single cell analysis, Survival Analysis, Receiver Operator Characteristics (ROC), Wizard for ProSeek protein analysis, and reader for 3D Gene microRNA analysis.

View our GenEx webpage and download a FREE GenEx 6 trial version => GenEx.Gene-Quantification.info

Join all GenEx talks by Prof. Mikael Kubista held at recent qPCR & NGS Events => www.eConferences.de/multid/

 

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages

 

   

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