Poster Sessions and Poster Numbers:

Next Generation Sequencing   P001 – P016. 3

 

Molecular Diagnostics   P017 – P035. 4

 

Molecular Diagnostics in Agriculture, Veterinary Medicine, and Environmental Science   P036 – P053. 6

 

New Application in qPCR   P054 – P071. 8

 

Digital PCR   P072 – P075. 10

 

Non-coding RNAs   P076 – P081. 10

 

qPCR Data Analysis   P082 – P090. 11

 

Assay Optimisation,  MIQE  and  Quality Control   P091 – P105. 12

 

 

Next Generation Sequencing

P001 – P016

 

P001

Exome Sequencing To Detect Novel Mutations Causing Malignant Hyperthermia

Anja H. Schiemann, Kathryn M. Stowell

Massey University, New Zealand


P002

Exploring the Complex Transcriptome of Pigmented and Non-pigmented Bovine Skin Using RNAseq

Rosemarie Weikard, Frieder Hadlich, Ronald Brunner, Christa Kühn

Leibniz Institute for Farm Animal Biology (FBN), Germany


P003

A Broad Re-sequencing Study Of 409 Genes In NCI-60 Cell Lines Using The Ion AmpliSeqTM Comprehensive Cancer Panel And Ion PGMTM Semiconductor Sequencing Reveals Previously Unreported Cell Line-specific Mutations

David Ruff, Benjamin Kong, Shiaw-Min Chen, Iris Casuga, David Joun, Chieh-Yuan Li, Rob Bennett, Mark Shannon

Life Technologies, United States of America

 

P004

Ready to Go for Next-Generation Sequencing: Extraction of Circulating microRNAs from Bovine Plasma

Melanie Spornraft1, Bettina Haase2, Vladimir Benes2, Michael W. Pfaffl1, Irmgard Riedmaier-Sprenzel1

1Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences, Technische Universität München, Freising, Germany2EMBL European Molecular Biology Laboratory, Genomics Core Facility, Heidelberg, Germany


P005

The predicted secretome in the transcriptome of cereal cyst nematode, Heterodera avenae 

Uma Rao, Prasoon Kumar Thakur, Mukesh Kumar, NagaVara Prasad G.

Indian Agricultural Research Institute (IARI), India


P006

Differences in microRNA composition over the course of lactation – Next-Generation-Sequencing of bovine milk and blood

Benedikt Kirchner1, Alexander Hahn2, Vladimir Benes3, Michael W Pfaffl1

1Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences Technische Universität München, Freising, German;2Genomatix Software GmbH, Munich, Germany; 3EMBL Genomics Core Facility (GeneCore), Heidelberg, Germany


P007

Rapid Sequencing Of The Entire Human mtDNA Using Next-Generation Sequencing By Ion Torrent PGM 

Simon Koren1, Mojca Tajnik2, Nataša Toplak1, Minka Kovač1, Damjan Glavač2

1Omega, d.o.o., Dolinškova 8, SI-1000 Ljubljana, Slovenia; 2University of Ljubljana, Faculty of Medicine, Department of Molecular Genetics, Korytkova 2, SI-1000 Ljubljana, Slovenia


P008

An Automated, High-Throughput Library Construction Protocol with Benefits for Low-Input Applications

Olaf Stelling1, Maryke Appel2Eric van der Walt2, John Foskett2, Olga Aminova3, Kety Huberman3, Adriana Heguy3

1Alpaqua Engineering, LLC, Beverly, MA, USA; 2Kapa Biosystems Inc., Woburn, MA, USA; 3Geoffrey Beene Translational Oncology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, USA


P009

Transcriptome Analysis on the Ion Proton™ System

Richard Fekete, Kelli Bramlett, Yongming Sun, Jeff Schageman, Luming Qu, Ross Hershorn, Charmaine San Jose Hinahon, Bob Setterquist

Life Technologies, United States of America

 

P010

Rapid whole genome sequencing investigation of a familial outbreak of E. coli O121:H19 with a sheep farm as the suspected source

Robert Söderlund1,2, Cecilia Jernberg3, Christine Källman2, Ingela Hedenström3, Erik Eriksson2, Erik Bongcam-Rudloff1, Anna Aspán2

1SLU Global Bioinformatics Centre, Swedish University of Agricultural Sciences, Uppsala, Sweden; 2National Veterinary Institute (SVA), Uppsala, Sweden; 3Swedish Institute for Communicable Disease Control (SMI), Solna, Sweden

 

P011

AmpliSeq™ RNA: Targeted sequencing of genes on the PGM ™

Richard Fekete, Brian Sanderson, Jeff Schageman, Angie Cheng, Kelli Bramlett

Life Technologies, United States of America


P012

High Resolution HLA Typing of Blood Stem Cell Donors by Next Generation Sequencing

Kaimo Hirv1, Thomas Zacher2Oliver Flieger3

1Center for Human Genetics and Laboratory Medicine, Dr. Klein & Dr. Rost, Martinsried, Germany; 2Hamilton Robotics GmbH, Martinsried, Germany; 3Hamilton Bonaduz AG, Bonaduz, Switzerland


P013

MuA Transposase Enzyme Enables Fast And Easy DNA Library Preparation For NGS

Laura-Leena Kiiskinen, Sanna Askolin, Julius Gagilas, Simona Gliebutė, Heli Haakana, Juuso Juhila, Ian Kavanagh, Arvydas Lubys, Justas Morkūnas, Minna Päiväsaari, Edita Povilaitienė, Jurgita RubekinaOssian Saris, Remigijus Skirgaila, Romas Tamoševičius, Gediminas Alzbutas, Mindaugas Ukanis

Thermo Fisher Scientific, Vantaa, Finland & Vilnius, Lithuania


P014

Next Generation Sequencing Sample Preparation Utilizing the Echo® Liquid Handler

Celeste Glazer, Jovica Pavlovic, Howard Lee, Danny Lee

Labcyte, United States of America

 

P015

Targeted Re-Sequencing Of 325 Inherited Disease- Associated Genes In A Family Trio And HapMap Populations Using The Ion Ampliseq™ Inherited Disease Panel And Ion PGM™ Semiconductor Sequencing

David Ruff, Iris Casuga, Benjamin Kong, David Joun, Shiaw-Min Chen, Chieh-Yuan Li, Alexander Joyner, Fiona Hyland, Rob Bennett, Mark Shannon

Life Technologies, United States of America


P016

Use of internal standard mixtures enables reliable NGS gene expression quantification at lower cost following multiple-round PCR  and inter-gene convergence of transcript abundance

Thomas Blomquist, Erin L. Crawford, James C. Willey

University of Toledo, United States of America

 

Molecular Diagnostics

P017 – P035

 

P017

Identification Of Distinct Subpopulations With Stem Cell Characteristics In Breast Cancer Types At Single Cell Level

Nina Akrap1, Eva Diffner1, Pernilla Gregersson1, Hannah Harrison2, Anders Ståhlberg1, Göran Landberg1,2

1Sahlgrenska Cancer Center, Gothenburg University, Sweden2Paterson Institute for Cancer Research, Manchester University, UK

 

P018

A multimarker qPCR platform for endometrial cancer biology characterisation

Anna Maria Supernat1,2, Zuzanna Urban1, Sylwia Łapińska-Szumczyk3, Sambor Sawicki3, Dariusz Wydra3, Anna Żaczek1

1Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk2Gdańsk Science and Technology Park, Pomeranian Special Economic Zone Ltd.; 3Department of Gynaecology, Gynaecological Oncology and Gynaecological Endocrinology, Medical University of Gdańsk


P019

Frequencies of CES1 Gene Polymorphisms In Patients With Metastatic Breast Cancer and Evaluation of Drug Response Effects in Capecitabine Users as Retrospectively 

Duygu Aygunes, Buket Kosova, Canfeza Sezgin

Ege University, Turkey


P020

Evaluation of a Microcapillary Electrophoresis (MCE)-Based Surveyor Scan KRAS Mutation Assay for the Pathology Routine

Rebekka Prokschi1, Gertrude Weberhofer2, Christa Freibauer3Alfred Schöller3

1University of Applied Sciences, Biomedical Science, Vienna, Austria2A. Menarini Diagnostics, Vienna, Austria3Institute of Clinical Pathology, LK Weinviertel Mistelbach-Gänserndorf, Mistelbach, Austria


P021

Development of a HRM-based detection method for DNA biomarkers in support of public health

Sigrid C. J. De Keersmaecker, Maud Delvoye, Omar Ahdach, Latifa El Bali, Nancy Roosens

Scientific Institute of Public Health (WIV-ISP), Belgium

 

P022

Fast And Cheap Screening Method Of A Novel Mutation In GJB6 Protein, Associated With  Non-Syndromic Hearing Loss

Nataša Toplak, Simon Koren, Minka Kovač

Omega d.o.o., Dolinškova 8, SI-1000 Ljubljana, Slovenia

 

P023

Effect of HPV-vaccination on HPV-prevalence in Belgium

Marusya Lieveld, Liesbeth Ceelen, Ramses Forsyth

Pathlicon, Belgium


P024

Upstream Open Reading Frames Are Regulated By Nicotinic Acetylcholine Receptor Subunits Associated With Smoking And Smoking-Related Disorders

Marlene Eggert1, Eric Aichinger1, Michael W Pfaffl2, Ortrud K Steinlein1, Martina Pfob1

1Institute of Human Genetics, University Hospital, Ludwig-Maximilians-University, Munich, Germany; 2Physiology Weihenstephan, Center of Life and Food Sciences Weihenstephan, Technical University of Munich, Germany


P025

Quantitative Allele Spesfic Amplification (quasa) In Residual Disease Monitoring Of Hariy Cell  Leukaemia 

Robert Powell1, Rebecca Gover1, Richard Ansell2, Sarah Bastow2, Tom Rider2, Helen Stewart2, Tim Chevassut2

1PrimerDesign Ltd, United Kingdom2Brighton & Sussex Medical School


P026

LoopTag Real-Time PCR Probe System for Sensitive Pathogen Detection

Henning Hanschmann2, Toni Kramer1,2, Thomas Juretzek4, Michael Steidle5, Christian Schröder1Stefan Rödiger1,2,3, Peter Schierack1, Werner Lehmann2

1Lausitz University of Applied Sciences, Germany; 2Attomol GmbH, Germany; 3Charité, Germany; 4Carl-Thiem-Klinikum Cottbus gGmbH, Germany; 5Laborärzte Sindelfingen, Germany


P027

Sensitive Methods for Detection of Secondary KIT Mutations in Gastrointestinal Stromal Tumours

Carina Heydt, Ulrike Koitzsch, Michaela Angelika Kleine, Helen Künstlinger, Eva Wardelmann, Margarete Odenthal, Sabine Merkelbach-Bruse

Institute of Pathology, University Hospital Cologne, Germany


P028

Cancer Biomarker Research Using castPCR™ Technology

David N. Keys, Yun Bao, Bonnie Ching, Mokang Mouanoutoua Mokang Mouanoutoua, Toinette Hartshorne, Sejal Desai, Junko Stevens, Caifu Chen

Life Technologies Genetic Analysis R&D. Foster City, CA. USA


P029

Validation of a real-time PCR method for SNPs analysis of known genes of lifestyle-related diseases among filipino adults

Mark Pretzel Zumaraga

Food and Nutrition Research Institute, Department of Science and Technology


P030

Cloning and Expression of Pakistani HCV NS3–4A Pprotease and Development of Robust in vitro Serine Protease Assay by FRET

Kaneez Fatima1, Steve Harakeh2, Afif Abdel Nour3, Ishtiaq Qadri4

1National University of Sciences & Technology, Islamabad, Pakistan,; 2Professor King Fahd Medical Research Center, King Abdul Aziz University, PO Box 80216 Jeddah 21589, Saudi Arabia,; 3Professor King Fahd Medical Research Center, King Abdul Aziz University, PO Box 80216 Jeddah 21589, Saudi Arabia,; 4Professor King Fahd Medical Research Center, King Abdul Aziz University, PO Box 80216 Jeddah 21589, Saudi Arabia,


P031

The Regulatory Effect of 1, α, 25-dihydroxyvitamin D3 on the Expression of Inflammatory Cytokines in Diabetic Retinopathy

Ali M Tohari, John A Craft, Xinhua Shu

Glasgow Caledonian University, United Kingdom


P032

Tumour Targeting of Gene Expression Using Hyaluronic Acid-Polypropylenimine Dendrimer Conjugates

Abdurrahim Elouzi1, Sandy A. Gray2, Andrease G. Schatzlein3, Ijeoma F. Uchegbu3

1Department of Pharmaceutics, Tripoli University, Tripoli, Libya; 2Department of Pharmaceutical Science, University of Strathclyde, Glasgow, UK;3UCL School of Pharmacy, University College London, London, UK

 

P033

Using a qPCR based method for screening several polymorphisms in COXI gene

Farid Ebnerasuly, Mohammad Said Hakhamaneshi, Naseh Sigari

Islamic Azad University Science and Research Branch, Iran, Islamic Republic of


P034

qPCR Gene Expression Profiles in Peripheral Blood Mononuclear Cells of Breast Cancer Patients

Ludek Sojka1,2, Irena Vancurova1,2, Pavel Strnad3, David Svec4, Mikael Kubista4, Radek Spisek1,2

1Sotio, a.s., Czech Republic; 2Department of Immunology, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic; 3Department of Gynecology and Obstetrics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic; 4TATAA Biocenter, Gothenburg, Sweden

 

P035

New Composites Biomarkers for Colorectal Cancer Diagnosis

Hicham Mansour1, Roberto Incitti1, Vladimir Bajic2

1Bioscience Laboratory, KAUST, Saudi Arabia; 2Computational Bioscience Center, KAUST, Saudi Arabia

 

Molecular Diagnostics in Agriculture, Veterinary Medicine, and Environmental Science

P036 – P053

 


P036

MALDI-TOF MS as confirmation tool for pathogens in drinking water.

Marsha van der Wiel, P. Willemse, G. Wubbels

WLN, Netherlands, The


P037

Quantification of Nitrifying and Manganese oxidizing bacteria with real-time QPCR to evaluate purification efficiency of sand filters in drinking water production

Marsha van der Wiel, Gerhard Wubbels

WLN, Netherlands, The


P038

Application of a Multiplex Real-time PCR Approach for the Quantification of Beef and Pork in Minced Meat and other Meat Products

Azuka Nkem Iwobi, Daniela Sebah, Gesche Fischer, Christoph Losher, Iris Kraemer, Georg Hauner, Ulrich Busch, Ingrid Huber

Bavarian Health and Food Safety Authority, Germany


P039

Study of Bacterial Diversity in the Topsoil and in the Hardpan in an Agricultural Soil by Metagenomics Following by Two Analysis Pipelines

Aurore Stroobants1Christophe Lambert2, Florine Degrune1, Daniel Portetelle1, Micheline Vandenbol1

1Unité de Microbiologie et Génomique, Université de Liège, Gembloux Agro-Bio Tech, Avenue Maréchal Juin 6, 5030 Gembloux, Belgium;2Progenus SA, Rue des Praules 2, 5030 Gembloux, Belgium


P040

Development of HRM assay for differentiation of Raspberry bushy dwarf virus (RBDV) coat protein sequence variants

Irena Mavrič Pleško1, Mojca Viršček Marn1, Minka Kovač2, Nataša Toplak2

1Kmetijski institut Slovenije, Slovenia2Omega d.o.o., Slovenia


P041

Development of a multiplex qPCR assay with propidium monoazide for quantifying viable Bifidobacterium animalis andLactococcus lactis in faeces.

Mickaël Boyer, Guillaume Gobert, Mélanie Laporte, Elodie Sadowski, Jérôme Combrisson

Danone Research, France


P042

Influence of sample particle size on subsequent steps in Real Time PCR GMO analysis

Monika Marković Borđoški, Gordana Nović, Danica Milinkov Guljaš

SP Laboratory, Serbia


P043

Detection and Quantification of Sulfate Reducing and Sulfur Oxidizing Bacteria in Corroded Wastewater Systems using Quantitative Real-Time PCR Technique

Bettina Huber1, Brigitte Helmreich1, Rolf König2, Elisabeth Müller1

1Institute of Water Quality Control, Am Coulombwall, 85748 Garching, TU München, Germany; 2Weber-Ingenieure GmbH, Stuttgarter Straße 115, 70469 Stuttgart, Germany


P044
Insights into the Microbiota of the Bovine Uterus

Lif Rødtness Vesterby Knudsen1, Cecilia Christensen Karstrup2, Kirstine Klitgaard Schou1, Hanne Gervi Pedersen2, Øystein Angen1, Jørgen Steen Agerholm2, Tim Kåre Jensen1

1Technical University of Denmark, Denmark2University of Copenhagen


P045

Quantification of infectious pathogenic  Campylobacter species in water using QPCR and PMA. 

Gerhard Herman Wubbels, Marsha v.d. Wiel, Auke Douma

Waterlaboratorium Noord, Netherlands, The


P046

Selection and validation of reference genes for quantitative gene expression studies by real-time PCR in brinjal (Solanum melongena L)

Uma Rao, NagaVara Prasad G., Pradeep Papolu, Prasoon Kumar Thakur, Divya Kamaraju, Rohini Srivathsa

Indian Agricultural Research Institute (IARI),India


P047

Preliminary Study For Screening Of Potentially Pathogenic Bacteria In Poultry Meat

Miha Kovac1,2, Nataša Toplak1, Simon Koren1, Minka Kovac1

1Omega d.o.o., Dolinškova 8, SI-1000 Ljubljana, Slovenia; 2University of Ljubljana, Veterinary Faculty, Gerbičeva 60, SI-Ljubljana, Slovenia


P048

Regulation of HIF-1alpha and vasohibins during follicle maturation and corpus luteum formation and function in bovine ovary

Bajram Berisha1,2, Stefanie Schilffarth2, Heike Kliem2, Michael W Pfaffl2, Dieter Schams2

1Faculty of Agriculture and Veterinary, University of Prishtina, Prishtinë, Kosovo; 2Physiology Weihenstephan, Technische Universität München, Freising, Germany


P049

Quantitative Analysis of Transcripts of the Open Reading Frames of Sugarcane Yellow Leaf Virus Genome by One-multiplex RT-PCR

Abdelaleim Ismail ElSayed

Biochemistry Department, Faculty of Agriculture, Zagazig University, Egypt


P050

Molecular characterization and phylogenetic relationships of newly discovered Potato virus X (PVX) strain from Peru 

Denis Kutnjak1, Rocio Silvestre2, Wilmer J. Cuellar2,3, Giovanna Müller2, Jan F. Kreuze2

1National Institute of Biology (NIB), Ljubljana, Slovenia2International Potato Center, Lima (CIP), Peru3International Center for Tropical Agriculture (CIAT), Cali, Colombia


P051

Mammary immunity of different cattle breeds measured with microfluidic high-throughput RT-qPCR

Diana Sorg1,2, Eike Fandrey3, Kai Frölich3, Heinrich H.D. Meyer1,2, Heike Kliem1,2

1Physiology Weihenstephan, Technische Universität München, Freising, Germany; 2ZIEL - Research Center for Nutrition and Food Sciences, Technische Universität München, Freising, Germany; 3Arche Warder, Zentrum für alte Haus- und Nutztierrassen e. V., Warder, Germany


P052

Influence of Nutrition Manipulation during an Early Growth Stage on Glucose Metabolisms of Longissimus Muscle  in Wagyu (Japanese Black)

Ryosuke Fujimura1, Kotaro Etoh1, Kunihiko Saitoh2, Kaori Metoki2, Shuichi Kaneda2, Takeshi Abe2, Tetsuji Etoh1, Yuji Shiotsuka1, Hidetoshi Suzuki2, Hiroyuki Hasebe2, Fumio Ebara1, Steffen Maak3, Elke Albrecht3, Akira Saito4, Takafumi Gotoh1

1Kuju Agricultural Research Center, Faculty of Agriculture, Kyushu University, Japan; 2National Livestock Breeding Center (NLBC), Japan;3Leibniz Institute for Farm Animal Biology, Germany; 4Zenrakuren, Japan


P053

The expression and localization of some lymphangiogenic VEGF family members in bovine corpus luteum

Bajram Berisha1,2, Rebecca Kenngott3, Stefanie Schilffarth2, Fred Sinowatz3, Dieter Schams2

1Faculty of Agriculture and Veterinary, University of Prishtina, Prishtinë, Kosovo; 2Physiology Weihenstephan, Technische Universität München, Freising, Germany; 3Institute of Veterinary Anatomy, Histology and Embryology, Ludwig-Maximilians-Universität München, München, Germany

 

New Application in qPCR

P054 – P071

 

P054

Novel mismatch suppression strategy enabling allele-specific cDNA synthesis and ultra-sensitive detection of RNA variants

Tho Huu Ho1,2, Lin Feng1,2, Sussana Lintula3, Arto K. Orpana4, Jakob Stenman1,5,6

1Minerva Foundation Institute for Medical Research, Helsinki, Finland; 2Faculty of Medicine, Helsinki University, Helsinki, Finland; 3Department of Clinical Chemistry, University of Helsinki, Helsinki, Finland; 4HUSLAB, Laboratory of Genetics, Helsinki, Finland; 5Institute for Molecular Medicine Finland,x Helsinki, Finland; 6Department of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden

 

P055

New holistic Approach for easy and cost efficient Genotyping of small numbers of Genes.

Michael M. Larsen1Ulf Bech Christensen2

1University of Southern Denmark; 2PentaBase ApS, Lumbyvej 11, building 5V, 5000 Odense C, Denmark


P056

An RT-qPCR Automated Platform: Validation Methods for the Access™ Workstation, the Echo® Liquid Handler, and RealTime Ready™ Reagents

Celeste Glazer, Randy Dyer, Sammy Datwani

Labcyte, United States of America

 

P057

Using TaqMan Assay Followed By Melting Analysis Performed By New-Generation Intercalating Dyes

Karel Bílek, Jiřina Procházková, Oldřich Kubíček

National Institute for NBC Protection, Czech Republic


P058

Attachment Of Embryonic Stem Cells-derivedCardiomyocytes In Cultispher-S Microcarriers By Using Spinner Flask

Abdulrhman Akasha

University of Tripoli, Libya

 

P059

High Throughput Profiling Of Antigen Specific T Cells: Repertoires In Vaccine Or Autoimmunity 

Anne Eugster, Annett Lindner, Anne-Kristin Heninger, Ezio Bonifacio

CRTD, TU Dresden, Germany


P060

Cost-effective real-time analysis by mediator probe (RT-)PCR

Simon Wadle1, Stefanie Rubenwolf1, Michael Lehnert1, Bernd Faltin2, Roland Zengerle1,3,4, Felix von Stetten1,3,4

1Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; 2Robert Bosch GmbH, Applied Research 1 - Microsystem Technologies - Microstructuring and Assembly, Postfach 10 60 50, 70049 Stuttgart, Germany; 3HSG-IMIT - Institut für Mikro- und Informationstechnik, Georges-Koehler-Allee 103, 79110 Freiburg, Germany;4BIOSS - Centre for Biological Signalling Studies, University of Freiburg, 79110 Freiburg, Germany


P061

Differential expression of heat shock protein genes after temperature treatment in Drosophila melanogaster andMegaselia scalaris at different stages of development

Tadeusz Malewski, Wiesław Bogdanowicz, Ewa Durska

Museum & Institute of Zoology, Poland


P062

Evaluation of the Effect of Magnetic Nanoparticles as Additives on qPCR

Marta Prado, Yury V. Kolen’ko, José Rivas

International Iberian Nanotechnology Laboratory, Portugal


P063

PCR never got so Cyxi - Lyophilised Mol Biol Reagents

Martin Alan Lee, Mark Laverick, Diane Rachel Lee

Fluorogenics LIMTED, United Kingdom


P064

Evaluation of a new plasma stabilisation technology for circulating cell-free DNA

Vlasta Korenková1, Lucie Langerová1, Mikael Kubista1,3, Francesca Salvianti2, Pamela Pinzani2

1Institute of Biotechnology, Academy of Sciences in Prague, v.v.i., Czech Republic (www.genex.ibt.cas.cz); 2University of Florence, Italy; 3TATAA Biocenter, Sweden (www.tataa.com)


P065

Single blastomere expression profiling revealed distribution of maternal transcripts in oocyte and daughter cells during early embryogenesis of Xenopus laevis.

Monika Flachsova1,3, Radek Sindelka1,2, Mikael Kubista1,4

1Institute of Biotechnology AS CR, v.v.i., Czech Republic; 2Whitehead Institute, Cambridge, USA; 3Charles University in Prague, Faculty of Science, Czech Republic; 4TATAA Biocenter AB, Goteborg, Sweden

 

P066

VideoScan - A Microscope Imaging Technology Platform for the Multiplex Real-Time PCR

Stefan Rödiger1,2,3, Peter Schierack1, Alexander Böhm1, Jörg Nitschke1, Wener Lehmann2, Christian Schröder1

1Lausitz University of Applied Sciences, Germany2Attomol GmbH, Germany3Charité, Germany


P067

Engineered DNA Polymerases for Future Applications

Ramon KranasterAndreas Marx

University of Konstanz, Germany


P068

Optimization of experiments using bisulfite pyrosequencing for gene-specific DNA methylation analyses

Veronika Leopoldine Pistek1,2, Jana Zecha1, Rainer Werner Fürst1, Susanne Ernestine Ulbrich1

1Physiology Weihenstephan, TUM, Germany2ZIEL PhD Graduate School `Nutritional Adaptation & Epigenetic Mechanisms´

P069

In Silico Design And In Vitro Validation Of 6-Plex RT-qPCR Assays

François Paillier1, Sylwia Kabacik2, Christophe Badie2

1bioMérieux, 5 rue des Berges, 38000, Grenoble, France2HPA Cancer Genetics and Cytogenetics group, Biological Effects department, Centre for Radiation, Chemical and Environmental Hazards, Chilton Didcot Oxon OX11 0RQ UK


P070

Next Generation Enzyme: A Multifunctional DNA Polymerase for Ultrafast Reverse Transcription

Nina Blatter, Jutta Mayer, Markus Wieland, Ramon Kranaster, Andreas Marx

Gründungsinitiative Prolago Biotec, Universität Konstanz


P071

Next Generation Enzymes for qPCR Applications

Gavin Rush, Eric van der Walt, John Foskett, Paul McEwan

Kapa Biosystems, Woburn, MA, USA

 

Digital PCR

P072 – P075

 

P072

Detection of Rare Somatic Mutations Using a Simplified, Specific Digital PCR Workflow with Zero Dead Volume.

David N. Keys, Anna Lam, Michael C. Pallas, Joyce Wilde, Jim C. Nurse, Iain Russell

Life Technologies, Foster City, CA, USA


P073

A Simulation Study To Assess The Variance Components In Absolute Quantification Using Digital Droplet PCR

Bart KM Jacobs, Els Goetghebeur, Lieven Clement

UGent, Belgium


P074

Digital PCR Modeling for Maximal Sensitivity, Dynamic Range and Measurement Precision

Nivedita Majumdar, Jeff Marks, Thomas Wessel

Life Technologies, United States of America


P075

Next Generation Digital PCR Technology: A  Simple, Chip-based Nanofluidic  system for any benchtop

Trish Hegerich, Joyce Wilde

Life Technologies, Foster City, CA, USA

 

Non-coding RNAs

P076 – P081

 

P076

Differences of miRNA Expression and DNA Modification Profile in Undifferentiated and Differentiated Mesenchymal Stem Cells

Kristina Daniunaite1, Roberta Misgirdaite1, Karolis Matjosaitis1, Kristina Stuopelyte1, Eiva Bernotiene2, Ausra Unguryte2, Sonata Jarmalaite1

1Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania; 2Centre of Innovative Medicine, State Institute of Scientific Research, Vilnius, Lithuania


P077

A Comprehensive Analysis of mRNA and microRNA Expression in Retinoic Acid treated 3T3-L1 Preadipocytes

Katharina Stöcker, Michael Pfaffl

Physiology Weihenstephan, TU München, Germany

 

P078

miRNA High-Throughput Sequencing in Endometriosis

Merli Saare1,2, Kadri Vaidla1,2, Triin Laisk-Podar2, Deniss Sõritsa4, Jaak Simm5, Agne Velthut-Meikas5, Helle Karro4, Madis Metsis2, Andrei Sõritsa6, Andres Salumets1,2,3, Maire Peters1,2

1University of Tartu, Department of Obstetrics and Gynecology, Tartu, Estonia; 2Competence Centre of Reproductive Medicine, Tartu, Estonia;3Institute of Biomedicine, University of Tartu, Estonia; 4Tartu University Hospital’s Women’s Clinic, Tartu, Estonia; 5Centre for Biology of Integrated Systems, Tallinn University of Technology, Estonia; 6Elite Clinic, Tartu, Estonia


P079

MicroRNA in biofluids – a case study on urine and the impact of optimized protocols.

Jörg Krummheuer, Thorarinn Blondal, Ditte Andreasen, Maria Wrang Teilum, Niels Tolstrup, Nana Jacobsen, Peter Mouritzen

Exiqon A/S, Skelstedet 16, DK-2950 Vedbaek, Denmark


P080

Identification of Differentially Expressed Genes and microRNAs in Myxoid Liposarcoma in order to Reveal New Therapeutic Target Structures

Helen Künstlinger1, Sebastian Dümcke2, Jana Fassunke1, Hans-Ulrich Schildhaus1, Michaela Angelika Kleine1, Carina Heydt1, Eva Wardelmann1, Gunhild Mechtersheimer3, Marcus Renner3, Achim Tresch2, Reinhard Büttner1, Sabine Merkelbach-Bruse1

1Institute of Pathology, University Hospital Cologne, Germany; 2Computational Biology and Regulatory Networks, Max Planck Institute for Plant Breeding Research Cologne, Germany; 3Institute of Pathology, University Hospital Heidelberg, Germany


P081

No Substantial Differences in Circulating microRNA Profile Throughout the Menstrual Cycle

Kadri Vaidla1,2, Merli Saare1,2,3, Agne Velthut-Meikas2,4, Jaak Simm4, Anne Mari Roost2, Madis Metsis2, Andres Salumets1,2,3, Maire Peters1,2

1Department of Obstetrics and Gynecology, University of Tartu, Estonia; 2Competence Centre on Reproductive Medicine and Biology, Estonia;3Institute of Biomedicine, University of Tartu, Estonia; 4Centre for Biology of Integrated Systems, Tallinn University of Technology

 

qPCR Data Analysis

P082 – P090

 

P082

How to do successful gene expression analysis using real-time PCR

Jan Hellemans1, Barbara D'haene1, Ariane De Ganck1, Jo Vandesompele1,2

1Biogazelle, Belgium2Center for Medical Genetics, Ghent University, Ghent, Belgium


P083

Simurealization Of Between Repeat Variability In Real Time PCR Reactions

Antoon Lievens1, Stefan Van Aelst1, Van den Bulcke Marc2, Goetghebeur Els1

1Ghent University, Belgium2Scientific Institute of Public Health, Belgium


P084

Advanced copy number variant analysis with qbasePLUS 

Barbara D'haene, Jo Vandesompele, Jan Hellemans

Biogazelle, Belgium


P085

The Neglected Role Of Statistical Weighting In Real-time qPCR Data.

Andrej-Nikolai Spiess

University Hospital Hamburg-Eppendorf, Germany


P086

Bioinformatics workflows with Snakemake

Johannes Köster1,2

1Genome Informatics, Faculty of Medicine, University Duisburg-Essen, Germany2Paediatric Oncology, University Hospital Essen, Germany


P087

Uncertainty Quantification In Real Time PCR Using Bootstrap

Stefan Van Aelst1, Antoon Lievens1, Marc Van den Bulcke2Els Goetghebeur1

1Ghent University, Belgium2Scientific Institute of Public Health, Belgium

 

P088

Analysis of Data from Experimental qPCR Systems with RKWard

Stefan Rödiger1,2,3, Alexander Böhm1, Jörg Nitschke1, Peter Schierack1, Werner Lehmann2, Ingolf Schimke3, Christian Schröder1

1Lausitz University of Applied Sciences, Germany2Attomol GmbH, Germany3Charité, Germany


P089

Advantages of Cy0 Quantification Using Spline Interpolation

Davide SistiMichele Guescini, Renato Panebianco, Marco B.L. Rocchi, Vilberto Stocchi

Department of Biomolecular Sciences, University of Urbino, Italy


P090

ChIP-qPCR and qbasePLUS  jointly identify a MYCN-activated miRNA cluster in cancer

Barbara D'haene1, Pieter Mestdagh1,2, Daniel Muth3, Frank Westerman3, Frank Speleman2, Jo Vandesompele1,2

1Biogazelle, Belgium2Center for Medical Genetics, Ghent University, Belgium3Department of Tumour Genetics, German Cancer Center, Heidelberg, Germany

 

Assay Optimisation,  MIQE  and  Quality Control

P091 - P105

 

P091

Improving biological relevancy of transcriptional biomarkers experiments by applying the MIQE guidelines to pre-clinical and clinical trials

Steve Harakeh1, Maxime Dooms2, Abalo Chango2, Esam Azhar1, Philippe Pouillart2, Afif Michel Abdel Nour1, Flore Depeint2

1KAU/KFRMC/Special Infectious Agents Unit, Biosafety Level 3, Saudi Arabia2Institut Polytechnique LaSalle Beauvais, Beauvais, France


P092

Analytical and Clinical Validation of In House qPCR for Detection of Human Papillomavirus (HPV) using MIQE Guidelines and ISO 15189

Daniela Cochicho, Luis Martins, Mário Cunha, Carmo Ornelas

Portuguese Institute of Oncology Lisbon, Francisco Gentil, EPE, Portugal


P093

Removal of Contaminating DNA from Commercial qPCR Master Mixes

Jorn Remi Henriksen, Elisabeth Lill Andreassen, Morten Elde

ArcticZymes AS, Tromsø, Norway

 

P094

A Quanatitative RT-PCR-based Approach To Assess cDNA Quality For Comparative Gene Expression Analysis

Bhaja Krushna Padhi, Marianela Rosales, Nicholas Huang, Guillaume Pelletier

Hazard Identification Division, Environmental Health Science and Research Bureau, HECSB, Health Canada, Ottawa, ON, Canada


P095

Assessing the quality and quantity of genomic DNA using the Agilent 2200 Tapestation and genomic DNA Screentape

Isabell Pechtl, Marcus Gassman, Donna McDade Walker, Rüdiger Salowsky

Agilent Technologies, Germany


P096

Development of a system based on SMART technology for robust transcriptome library preparation from small quantities of degraded sample

Francois-Xavier Sicot1, Magnolia Bostick2, Cynthia Chang2, Tommy Duong2, Andrew Farmer2

1Takara Bio Europe, France2Clontech Laboratories, Inc. CA, USA

 

P097

Nucleic acid purification with magnetic particle processor prior to qPCR

Eva Tas, Marika Suomalainen, Ossian Saris, Sini Suomalainen

Thermo Fisher Scientific, Vantaa, Finland


P098

Reference Gene Selection And Validation For HT29 And VK2/E6E7 Human Epithelial Cell Lines Treated With Probiotic And Pathogenic Bacteria

Annette Vivi Jacobsen1,2, Bisrat Tewelde1, Jana Jass1, Nikolai Scherbak1

1Örebro University, Sweden2Charles Sturt University, Australia


P099

Optimization of the DNA analysis workflow by applying an automated CTAB buffer-based DNA extraction prior to qPCR

Patrick Guertler1, Andrea Harwardt2, Adelina Eicheldinger1, Paul Muschler3, Ottmar Goerlich1, Ulrich Busch1

1Bavarian Health and Food Safety Authority, Germany2Synlab Labordienstleistungen, Germany3Promega GmbH, Germany

 

P100

MIQE in colic microbiota monitoring for clinical and preclinical trials

Maxime Dooms2, Thierry Ribeiro2, Esam Azhar1, Steve Harakeh1, Flore Depeint2Afif Michel Abdel Nour1

1KAU/KFRMC/Special Infectious Agents Unit, Biosafety Level 3, Saudi Arabia2Institut Polytechnique LaSalle Beauvais, Beauvais, France


P101

Quality Control of DNA from Formalin-Fixed Paraffin-Embedded and Fresh-Frozen Tissues Prior to Target-Enrichment and Next Generation Sequencing 

Isabell Pechtl, Melissa Liu, Maria Celeste Ramirez, Rüdiger Salowsky

Agilent Technologies, Germany


P102

High Throughput Miniaturized PCR using the Echo® 525 Liquid Handler

Celeste Glazer, Carl Jarman, Brent Eaton

Labcyte, United States of America


P103

Quality control of RNA using the Agilent 2200 TapeStation system

Isabell Pechtl, Arunkumar Padmanaban, Rüdiger Salowsky, Charmian Cher

Agilent Technologies, Germany


P104

Quantification of GMO at a Level of 0.1 % – A Statistical Approach Using Frequency Distribution

Lars Gerdes, Ulrich Busch, Sven Pecoraro

Bavarian Health and Food Safety Authority (LGL), Germany


P105

Gene Expression Profiling: qPCR Toolkit for Quality Control

David Svec1, Jenny Jacobsson1, Robert Sjöback1, Mikael Kubista1,2

1TATAA Biocenter, Sweden2IBT, Academy of Sciences, Czech Republic