Poster Sessions and
Poster Numbers:
Next Generation
Sequencing P001 – P016
Molecular
Diagnostics P017 – P035
Molecular
Diagnostics in Agriculture, Veterinary Medicine, and Environmental Science P036 – P053
New Application
in qPCR P054 – P071
qPCR Data
Analysis P082 – P090
Assay
Optimisation, MIQE and
Quality Control P091 – P105
Next
Generation Sequencing
P001 – P016 |
P001 Exome Sequencing To Detect Novel Mutations Causing
Malignant Hyperthermia Anja H. Schiemann, Kathryn M. Stowell P002 Exploring
the Complex Transcriptome of Pigmented and Non-pigmented Bovine Skin Using RNAseq Rosemarie
Weikard, Frieder Hadlich, Ronald Brunner, Christa Kühn Leibniz Institute
for Farm Animal Biology (FBN), P003 A
Broad Re-sequencing Study Of 409 Genes In NCI-60 Cell Lines Using The Ion AmpliSeqTM Comprehensive Cancer Panel And
Ion PGMTM Semiconductor Sequencing Reveals Previously
Unreported Cell Line-specific Mutations David
Ruff, Benjamin Kong, Shiaw-Min Chen, Iris Casuga,
David Joun, Chieh-Yuan
Li, Rob Bennett, Mark Shannon Life Technologies, P004 Ready
to Go for Next-Generation Sequencing: Extraction of Circulating microRNAs
from Bovine Plasma Melanie Spornraft1,
Bettina Haase2, Vladimir Benes2, Michael W. Pfaffl1,
Irmgard Riedmaier-Sprenzel1 1Physiology Weihenstephan, P005 The
predicted secretome in the transcriptome of cereal
cyst nematode, Heterodera avenae Uma Rao, Prasoon
Kumar Thakur, Mukesh
Kumar, NagaVara Prasad G. Indian Agricultural
Research Institute (IARI), P006 Differences
in microRNA composition over the course of lactation –
Next-Generation-Sequencing of bovine milk and blood Benedikt Kirchner1,
Alexander Hahn2, Vladimir Benes3, Michael W Pfaffl1 1Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences Technische Universität München, Freising,
German;2Genomatix Software GmbH, Munich,
Germany; 3EMBL Genomics Core Facility
(GeneCore), Heidelberg, Germany P007 Rapid Sequencing Of The Entire Human mtDNA Using Next-Generation Sequencing By Ion Torrent PGM Simon
Koren1, Mojca Tajnik2,
Nataša Toplak1, Minka
Kovač1, Damjan Glavač2 1Omega, d.o.o.,
Dolinškova 8, SI-1000 Ljubljana, Slovenia; 2University
of Ljubljana, Faculty of Medicine, Department of Molecular Genetics, Korytkova 2, SI-1000 Ljubljana, Slovenia P008 An
Automated, High-Throughput Library Construction Protocol with Benefits for
Low-Input Applications Olaf
Stelling1, Maryke Appel2, Eric
van der Walt2, John Foskett2,
Olga Aminova3, Kety Huberman3,
Adriana Heguy3 1Alpaqua Engineering, LLC, Beverly, MA,
USA; 2Kapa Biosystems Inc., Woburn, MA, USA; 3Geoffrey
Beene Translational Oncology Core Facility,
Memorial Sloan Kettering Cancer Center, New York, NY, USA P009 Transcriptome
Analysis on the Ion Proton™ System Richard
Fekete, Kelli Bramlett, Yongming Sun, Jeff Schageman, Luming Qu, Ross Hershorn, Charmaine San Jose Hinahon, Bob Setterquist Life Technologies, P010 Rapid
whole genome sequencing investigation of a familial outbreak of E.
coli O121:H19 with a sheep farm as the suspected source Robert
Söderlund1,2, Cecilia Jernberg3,
Christine Källman2, Ingela Hedenström3,
Erik Eriksson2, Erik Bongcam-Rudloff1, Anna Aspán2 1SLU Global Bioinformatics Centre, Swedish
University of Agricultural Sciences, Uppsala, Sweden; 2National
Veterinary Institute (SVA), Uppsala, Sweden; 3Swedish
Institute for Communicable Disease Control (SMI), Solna,
Sweden P011 AmpliSeq™ RNA: Targeted sequencing of genes on the PGM ™ Richard Fekete, Brian Sanderson, Jeff Schageman,
Angie Cheng, Kelli Bramlett Life Technologies, P012 High Resolution HLA Typing of Blood Stem
Cell Donors by Next Generation Sequencing Kaimo Hirv1, Thomas Zacher2, Oliver
Flieger3 1Center for Human Genetics and Laboratory
Medicine, Dr. Klein & Dr. Rost, Martinsried,
Germany; 2Hamilton Robotics GmbH, Martinsried, Germany; 3Hamilton
Bonaduz AG, Bonaduz,
Switzerland P013 MuA Transposase
Enzyme Enables Fast And Easy DNA Library Preparation For NGS Laura-Leena Kiiskinen, Sanna Askolin, Julius Gagilas, Simona Gliebutė, Heli Haakana, Juuso Juhila, Ian Kavanagh, Arvydas Lubys, Justas Morkūnas, Minna Päiväsaari, Edita Povilaitienė, Jurgita Rubekina, Ossian Saris, Remigijus
Skirgaila, Romas Tamoševičius, Gediminas Alzbutas, Mindaugas Ukanis Thermo Fisher
Scientific, P014 Next Generation Sequencing Sample
Preparation Utilizing the Echo® Liquid Handler Celeste
Glazer, Jovica Pavlovic,
Howard Lee, Danny Lee P015 Targeted
Re-Sequencing Of 325 Inherited Disease- Associated Genes In A Family
Trio And HapMap Populations Using The Ion Ampliseq™ Inherited Disease Panel And Ion PGM™
Semiconductor Sequencing David
Ruff, Iris Casuga, Benjamin Kong, David Joun,
Shiaw-Min Chen, Chieh-Yuan
Li, Alexander Joyner, Fiona Hyland, Rob Bennett, Mark Shannon Life Technologies, P016 Use
of internal standard mixtures enables reliable NGS gene expression
quantification at lower cost following multiple-round PCR and
inter-gene convergence of transcript abundance Thomas Blomquist, Erin L. Crawford, James C. Willey |
Molecular
Diagnostics
P017 – P035 |
P017 Identification
Of Distinct Subpopulations With Stem Cell Characteristics In Breast Cancer
Types At Single Cell Level Nina
Akrap1, Eva Diffner1, Pernilla Gregersson1, Hannah Harrison2,
Anders Ståhlberg1, Göran Landberg1,2 1Sahlgrenska P018 A multimarker qPCR platform for endometrial cancer biology characterisation Anna
Maria Supernat1,2, Zuzanna
Urban1, Sylwia
Łapińska-Szumczyk3, Sambor
Sawicki3, Dariusz Wydra3,
Anna Żaczek1 1Department of Medical Biotechnology,
Intercollegiate Faculty of Biotechnology, University of Gdańsk
and Medical University of Gdańsk; 2Gdańsk
Science and Technology Park, Pomeranian Special Economic Zone Ltd.; 3Department
of Gynaecology, Gynaecological
Oncology and Gynaecological Endocrinology, Medical
University of Gdańsk P019 Frequencies
of CES1 Gene Polymorphisms In Patients With Metastatic Breast Cancer and
Evaluation of Drug Response Effects in Capecitabine
Users as Retrospectively Duygu Aygunes, Buket Kosova, Canfeza Sezgin P020 Evaluation of a
Microcapillary Electrophoresis (MCE)-Based Surveyor
Scan KRAS Mutation Assay for the Pathology Routine Rebekka Prokschi1, Gertrude Weberhofer2,
Christa Freibauer3, Alfred Schöller3 1University of Applied Sciences,
Biomedical Science, P021 Development
of a HRM-based detection method for DNA biomarkers in support of public
health Sigrid
C. J. De Keersmaecker, Maud Delvoye,
Omar Ahdach, Latifa El
Bali, Nancy Roosens Scientific P022 Fast
And Cheap Screening Method Of A Novel Mutation In GJB6 Protein, Associated
With Non-Syndromic Hearing Loss Nataša Toplak, Simon Koren,
Minka Kovač Omega d.o.o., Dolinškova 8, SI-1000
Ljubljana, Slovenia P023 Effect of HPV-vaccination on
HPV-prevalence in Belgium Marusya Lieveld, Liesbeth Ceelen, Ramses Forsyth P024 Marlene Eggert1, Eric
Aichinger1, Michael W Pfaffl2, Ortrud K Steinlein1,
Martina Pfob1 1Institute of Human Genetics, University
Hospital, Ludwig-Maximilians-University, Munich,
Germany; 2Physiology Weihenstephan, Center of Life and Food
Sciences Weihenstephan, Technical University of Munich, Germany P025 Robert
Powell1, Rebecca Gover1, Richard
Ansell2, Sarah Bastow2, Tom Rider2, Helen
Stewart2, Tim Chevassut2 1PrimerDesign Ltd, P026 LoopTag Real-Time PCR Probe System for Sensitive
Pathogen Detection Henning Hanschmann2, Toni Kramer1,2,
Thomas Juretzek4, Michael Steidle5, Christian Schröder1, Stefan
Rödiger1,2,3, Peter Schierack1, Werner Lehmann2 1Lausitz University of Applied Sciences,
Germany; 2Attomol GmbH, Germany; 3Charité,
Germany; 4Carl-Thiem-Klinikum Cottbus gGmbH,
Germany; 5Laborärzte Sindelfingen, Germany P027 Sensitive
Methods for Detection of Secondary KIT Mutations in
Gastrointestinal Stromal Tumours Carina Heydt, Ulrike
Koitzsch, Michaela Angelika Kleine, Helen Künstlinger, Eva Wardelmann,
Margarete Odenthal, Sabine Merkelbach-Bruse P028 Cancer
Biomarker Research Using castPCR™ Technology David
N. Keys, Yun Bao, Bonnie Ching, Mokang Mouanoutoua Mokang Mouanoutoua, Toinette
Hartshorne, Sejal Desai, Junko Stevens, Caifu Chen Life Technologies
Genetic Analysis R&D. Foster City, CA. P029 Validation
of a real-time PCR method for SNPs analysis of known genes of
lifestyle-related diseases among filipino adults Mark
Pretzel Zumaraga Food and Nutrition
Research Institute, Department of Science and Technology P030 Cloning
and Expression of Pakistani HCV NS3–4A Pprotease
and Development of Robust in vitro Serine Protease Assay by FRET Kaneez Fatima1, Steve Harakeh2, Afif Abdel Nour3,
Ishtiaq Qadri4 1National University of Sciences &
Technology, Islamabad, Pakistan,; 2Professor King Fahd Medical
Research Center, King Abdul Aziz University, PO Box 80216 Jeddah 21589, Saudi
Arabia,; 3Professor King Fahd Medical Research Center, King
Abdul Aziz University, PO Box 80216 Jeddah 21589, Saudi Arabia,; 4Professor
King Fahd Medical Research Center, King Abdul Aziz University, PO Box 80216
Jeddah 21589, Saudi Arabia, P031 Ali M Tohari, John A Craft, Xinhua Shu P032 Tumour Targeting of Gene Expression Using Hyaluronic Acid-Polypropylenimine
Dendrimer Conjugates Abdurrahim Elouzi1, Sandy A. Gray2, Andrease
G. Schatzlein3, Ijeoma F. Uchegbu3 1Department of Pharmaceutics, Tripoli
University, Tripoli, Libya; 2Department of Pharmaceutical
Science, University of Strathclyde, Glasgow, UK;3UCL
School of Pharmacy, University College London, London, UK P033 Using
a qPCR based method for screening several polymorphisms in COXI gene Farid Ebnerasuly, Mohammad Said Hakhamaneshi,
Naseh Sigari Islamic P034 qPCR Gene Expression Profiles in
Peripheral Blood Mononuclear Cells of Breast Cancer Patients Ludek Sojka1,2, Irena Vancurova1,2, Pavel
Strnad3, David Svec4, Mikael Kubista4, Radek Spisek1,2 1Sotio, a.s.,
Czech Republic; 2Department of Immunology, 2nd Faculty of
Medicine, Charles University and University Hospital Motol,
Prague, Czech Republic; 3Department of Gynecology and
Obstetrics, 2nd Faculty of Medicine, Charles University and University
Hospital Motol, Prague, Czech Republic; 4TATAA
Biocenter, Gothenburg, Sweden P035 New Composites Biomarkers for Colorectal
Cancer Diagnosis 1Bioscience Laboratory, KAUST, |
Molecular
Diagnostics in Agriculture, Veterinary Medicine, and Environmental Science
P036 – P053 |
P036 MALDI-TOF MS as confirmation tool for
pathogens in drinking water. Marsha van der Wiel, P. Willemse, G. Wubbels WLN, P037 Marsha van der Wiel,
Gerhard Wubbels WLN, P038 Application
of a Multiplex Real-time PCR Approach for the Quantification of Beef and Pork
in Minced Meat and other Meat Products Azuka Nkem Iwobi,
Daniela Sebah, Gesche Fischer, Christoph Losher, Iris Kraemer, Georg Hauner,
Ulrich Busch, Ingrid Huber Bavarian Health and
Food Safety P039 Study
of Bacterial Diversity in the Topsoil and in the Hardpan in an Agricultural
Soil by Metagenomics Following by Two Analysis
Pipelines Aurore Stroobants1, Christophe Lambert2,
Florine Degrune1, Daniel Portetelle1,
Micheline Vandenbol1 1Unité de Microbiologie
et Génomique, Université
de Liège, Gembloux
Agro-Bio Tech, Avenue Maréchal Juin
6, 5030 Gembloux, Belgium;2Progenus SA,
Rue des Praules 2, 5030 Gembloux,
Belgium P040 Development
of HRM assay for differentiation of Raspberry bushy dwarf virus (RBDV)
coat protein sequence variants Irena Mavrič Pleško1, Mojca Viršček Marn1, Minka
Kovač2, Nataša Toplak2 1Kmetijski institut
P041 Development
of a multiplex qPCR assay with propidium monoazide
for quantifying viable Bifidobacterium
animalis andLactococcus
lactis in faeces. Mickaël Boyer, Guillaume Gobert, Mélanie Laporte, Elodie Sadowski, Jérôme Combrisson P042 Influence of sample particle size on
subsequent steps in Real Time PCR GMO analysis Monika Marković Borđoški, Gordana Nović, Danica Milinkov Guljaš SP Laboratory, P043 Bettina Huber1,
Brigitte Helmreich1, Rolf König2, Elisabeth Müller1 1Institute of Water Quality Control, Am Coulombwall, 85748 Garching, TU
München, Germany; 2Weber-Ingenieure GmbH, Stuttgarter
Straße 115, 70469 Stuttgart, Germany P044 Lif Rødtness Vesterby Knudsen1, Cecilia Christensen Karstrup2, Kirstine Klitgaard Schou1,
Hanne Gervi Pedersen2, Øystein Angen1, Jørgen
Steen Agerholm2, Tim Kåre Jensen1 1Technical P045 Quantification of infectious pathogenic Campylobacter species in water using QPCR and
PMA. Gerhard Herman Wubbels, Marsha
v.d. Wiel, Auke Douma P046 Selection
and validation of reference genes for quantitative gene expression studies by
real-time PCR in brinjal (Solanum
melongena L) Uma Rao, NagaVara
Prasad G., Pradeep Papolu,
Prasoon Kumar Thakur, Divya Kamaraju, Rohini Srivathsa Indian Agricultural
Research Institute (IARI), P047 Preliminary Study For Screening Of
Potentially Pathogenic Bacteria In Poultry Meat Miha Kovac1,2, Nataša Toplak1,
Simon Koren1, Minka Kovac1 1Omega d.o.o., Dolinškova 8, SI-1000 Ljubljana, Slovenia; 2University
of Ljubljana, Veterinary Faculty, Gerbičeva
60, SI-Ljubljana, Slovenia P048 Regulation
of HIF-1alpha and vasohibins during follicle
maturation and corpus luteum formation and function in bovine ovary Bajram Berisha1,2,
Stefanie Schilffarth2, Heike Kliem2, Michael W Pfaffl2,
Dieter Schams2 1Faculty of Agriculture and Veterinary, P049 Quantitative
Analysis of Transcripts of the Open Abdelaleim Ismail ElSayed Biochemistry
Department, Faculty of Agriculture, P050 Denis Kutnjak1, Rocio Silvestre2, Wilmer
J. Cuellar2,3, Giovanna Müller2, Jan F. Kreuze2 1National P051 Mammary immunity of different cattle
breeds measured with microfluidic high-throughput RT-qPCR Diana Sorg1,2, Eike
Fandrey3, Kai Frölich3, Heinrich H.D. Meyer1,2,
Heike Kliem1,2 1Physiology Weihenstephan, Technische Universität München,
Freising, Germany; 2ZIEL - Research Center for Nutrition and Food Sciences, Technische Universität München, Freising,
Germany; 3Arche Warder, Zentrum für
alte Haus- und Nutztierrassen e. V., Warder,
Germany P052 Influence
of Nutrition Manipulation during an Early Growth Stage on Glucose Metabolisms
of Longissimus Muscle in Wagyu
(Japanese Black) Ryosuke Fujimura1, Kotaro Etoh1, Kunihiko
Saitoh2, Kaori Metoki2, Shuichi Kaneda2,
Takeshi Abe2, Tetsuji Etoh1,
Yuji Shiotsuka1, Hidetoshi Suzuki2, Hiroyuki Hasebe2,
Fumio Ebara1, Steffen Maak3, Elke
Albrecht3, Akira Saito4, Takafumi
Gotoh1 1Kuju Agricultural Research Center,
Faculty of Agriculture, Kyushu University, Japan; 2National
Livestock Breeding Center (NLBC), Japan;3Leibniz Institute for
Farm Animal Biology, Germany; 4Zenrakuren, Japan P053 The
expression and localization of some lymphangiogenic
VEGF family members in bovine corpus luteum Bajram Berisha1,2,
Rebecca Kenngott3, Stefanie Schilffarth2, Fred Sinowatz3,
Dieter Schams2 1Faculty of Agriculture and Veterinary,
University of Prishtina, Prishtinë, Kosovo; 2Physiology
Weihenstephan, Technische Universität München, Freising, Germany; 3Institute
of Veterinary Anatomy, Histology and Embryology, Ludwig-Maximilians-Universität
München, München, Germany |
New Application in qPCR
P054 – P071 |
P054 Novel
mismatch suppression strategy enabling allele-specific cDNA synthesis and
ultra-sensitive detection of RNA variants Tho Huu Ho1,2, Lin Feng1,2, Sussana
Lintula3, Arto K. Orpana4, Jakob Stenman1,5,6 1Minerva Foundation Institute for Medical
Research, Helsinki, Finland; 2Faculty of Medicine, Helsinki
University, Helsinki, Finland; 3Department of Clinical
Chemistry, University of Helsinki, Helsinki, Finland; 4HUSLAB,
Laboratory of Genetics, Helsinki, Finland; 5Institute for Molecular
Medicine Finland,x Helsinki, Finland; 6Department
of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden P055 New holistic Approach for easy and cost
efficient Genotyping of small numbers of Genes. Michael
M. Larsen1, Ulf Bech Christensen2 1University of Southern Denmark; 2PentaBase
ApS, Lumbyvej 11,
building 5V, 5000 Odense C, P056 Celeste
Glazer, Randy Dyer, Sammy
Datwani P057 Using TaqMan
Assay Followed By Melting Analysis Performed By New-Generation Intercalating
Dyes Karel Bílek, Jiřina Procházková, Oldřich Kubíček National Institute
for NBC Protection, P058 Attachment
Of Embryonic Stem Cells-derivedCardiomyocytes In Cultispher-S Microcarriers By Using
Spinner Flask Abdulrhman Akasha P059 High
Throughput Profiling Of Antigen Specific T Cells: Repertoires In Vaccine Or
Autoimmunity Anne Eugster, Annett
Lindner, Anne-Kristin Heninger, Ezio Bonifacio CRTD, TU P060 Cost-effective real-time analysis by
mediator probe (RT-)PCR Simon Wadle1,
Stefanie Rubenwolf1, Michael Lehnert1, Bernd Faltin2,
Roland Zengerle1,3,4, Felix von Stetten1,3,4 1Laboratory for MEMS Applications, IMTEK
- Department of Microsystems Engineering, University of Freiburg,
Georges-Koehler-Allee 103, 79110 Freiburg,
Germany; 2Robert Bosch GmbH, Applied Research 1 - Microsystem Technologies - Microstructuring
and Assembly, Postfach 10 60 50, 70049 Stuttgart,
Germany; 3HSG-IMIT - Institut für Mikro- und Informationstechnik,
Georges-Koehler-Allee 103, 79110 Freiburg, Germany;4BIOSS
- Centre for Biological Signalling Studies,
University of Freiburg, 79110 Freiburg, Germany P061 Differential
expression of heat shock protein genes after temperature treatment in Drosophila
melanogaster andMegaselia
scalaris at different stages of
development Tadeusz Malewski, Wiesław
Bogdanowicz, Ewa Durska Museum &
Institute of P062 Evaluation of the Effect of Magnetic Nanoparticles as Additives on qPCR Marta
Prado, Yury V. Kolen’ko, José Rivas International
Iberian Nanotechnology P063 PCR never got so Cyxi
- Lyophilised Mol Biol
Reagents Martin
Alan Lee, Mark Laverick, Diane Rachel Lee P064 Evaluation
of a new plasma stabilisation technology for
circulating cell-free DNA Vlasta Korenková1, Lucie Langerová1, Mikael Kubista1,3,
Francesca Salvianti2, Pamela Pinzani2 1Institute of Biotechnology, Academy of
Sciences in Prague, v.v.i., Czech Republic
(www.genex.ibt.cas.cz); 2University of Florence, Italy; 3TATAA
Biocenter, Sweden (www.tataa.com) P065 Single
blastomere expression profiling revealed
distribution of maternal transcripts in oocyte and daughter cells during
early embryogenesis of Xenopus laevis. Monika Flachsova1,3, Radek
Sindelka1,2, Mikael Kubista1,4 1Institute of Biotechnology AS CR, v.v.i., Czech Republic; 2Whitehead
Institute, Cambridge, USA; 3Charles University in Prague,
Faculty of Science, Czech Republic; 4TATAA Biocenter AB,
Goteborg, Sweden P066 VideoScan - A Microscope Imaging Technology
Platform for the Multiplex Real-Time PCR Stefan Rödiger1,2,3, Peter
Schierack1, Alexander Böhm1, Jörg Nitschke1,
Wener Lehmann2, Christian Schröder1 P067 Engineered
DNA Polymerases for Future Applications Ramon Kranaster, Andreas Marx P068 Optimization
of experiments using bisulfite pyrosequencing for
gene-specific DNA methylation analyses Veronika Leopoldine
Pistek1,2, Jana Zecha1, Rainer Werner
Fürst1, Susanne Ernestine Ulbrich1 1Physiology Weihenstephan, TUM, P069 In
Silico Design
And In Vitro Validation Of 6-Plex RT-qPCR Assays François
Paillier1, Sylwia
Kabacik2, Christophe Badie2 1bioMérieux, 5 rue des Berges, 38000, P070 Next
Generation Enzyme: A Multifunctional DNA Polymerase for Ultrafast Reverse
Transcription Nina Blatter, Jutta Mayer, Markus Wieland, Ramon
Kranaster, Andreas Marx Gründungsinitiative Prolago Biotec, Universität Konstanz P071 Next
Generation Enzymes for qPCR Applications Gavin Rush, Eric van der Walt, John Foskett, Paul McEwan Kapa
Biosystems, Woburn, MA, USA |
Digital
PCR
P072
– P075 |
P072 Detection
of Rare Somatic Mutations Using a Simplified, Specific Digital PCR Workflow
with Zero Dead Volume. David
N. Keys, Anna Lam, Michael
C. Pallas, Joyce Wilde, Jim C. Nurse, Iain Russell Life Technologies, P073 Bart KM
Jacobs, Els Goetghebeur, Lieven Clement P074 Digital
PCR Modeling for Maximal Sensitivity, Nivedita Majumdar, Jeff Marks, Thomas Wessel Life Technologies, P075 Next
Generation Digital PCR Technology: A Simple,
Chip-based Nanofluidic system for any benchtop. Trish Hegerich, Joyce Wilde Life Technologies, |
Non-coding
RNAs
P076
– P081 |
P076 Differences
of miRNA Expression and DNA Modification Profile in Undifferentiated and
Differentiated Mesenchymal Stem Cells Kristina
Daniunaite1, Roberta Misgirdaite1, Karolis Matjosaitis1, Kristina Stuopelyte1,
Eiva Bernotiene2, Ausra
Unguryte2, Sonata Jarmalaite1 1Human Genome Research Centre, Faculty of
Natural Sciences, Vilnius University, Vilnius, Lithuania; 2Centre
of Innovative Medicine, State Institute of Scientific Research, Vilnius,
Lithuania P077 A
Comprehensive Analysis of mRNA and microRNA Expression in Retinoic Acid
treated 3T3-L1 Preadipocytes Katharina Stöcker, Michael Pfaffl Physiology
Weihenstephan, TU München, Germany P078 miRNA
High-Throughput Sequencing in Endometriosis Merli Saare1,2, Kadri Vaidla1,2,
Triin Laisk-Podar2, Deniss
Sõritsa4, Jaak Simm5, Agne Velthut-Meikas5, Helle
Karro4, Madis Metsis2, Andrei
Sõritsa6, Andres Salumets1,2,3, Maire
Peters1,2 1University of Tartu, Department of
Obstetrics and Gynecology, Tartu, Estonia; 2Competence Centre
of Reproductive Medicine, Tartu, Estonia;3Institute of
Biomedicine, University of Tartu, Estonia; 4Tartu University
Hospital’s Women’s Clinic, Tartu, Estonia; 5Centre for
Biology of Integrated Systems, Tallinn University of Technology,
Estonia; 6Elite Clinic, Tartu, Estonia P079 MicroRNA
in biofluids – a case study on urine and the impact
of optimized protocols. Jörg Krummheuer, Thorarinn Blondal, Ditte Andreasen, Maria Wrang Teilum, Niels Tolstrup, Nana Jacobsen, Peter Mouritzen Exiqon A/S, Skelstedet 16, DK-2950 P080 Identification
of Differentially Expressed Genes and microRNAs in Myxoid
Liposarcoma in order to Reveal New Therapeutic
Target Structures Helen Künstlinger1,
Sebastian Dümcke2, Jana Fassunke1, Hans-Ulrich
Schildhaus1, Michaela Angelika Kleine1, Carina Heydt1,
Eva Wardelmann1, Gunhild Mechtersheimer3, Marcus Renner3,
Achim Tresch2, Reinhard Büttner1, Sabine
Merkelbach-Bruse1 1Institute of Pathology, University
Hospital Cologne, Germany; 2Computational Biology and
Regulatory Networks, Max Planck Institute for Plant Breeding Research
Cologne, Germany; 3Institute of Pathology, University
Hospital Heidelberg, Germany P081 No
Substantial Differences in Circulating microRNA Profile Throughout the
Menstrual Cycle Kadri Vaidla1,2, Merli Saare1,2,3,
Agne Velthut-Meikas2,4, Jaak Simm4, Anne Mari Roost2, Madis Metsis2, Andres Salumets1,2,3,
Maire Peters1,2 1Department of Obstetrics and Gynecology,
University of Tartu, Estonia; 2Competence Centre on
Reproductive Medicine and Biology, Estonia;3Institute of Biomedicine,
University of Tartu, Estonia; 4Centre for Biology of
Integrated Systems, Tallinn University of Technology |
qPCR Data Analysis
P082
– P090 |
P082 How
to do successful gene expression analysis using real-time PCR Jan
Hellemans1, Barbara D'haene1, Ariane De
Ganck1, Jo Vandesompele1,2 1Biogazelle, P083 Simurealization Of Between Repeat Variability In Real
Time PCR Reactions Antoon Lievens1, Stefan Van Aelst1, Van den Bulcke Marc2, Goetghebeur
Els1 P084 Advanced
copy number variant analysis with qbasePLUS Barbara
D'haene, Jo Vandesompele, Jan Hellemans P085 The
Neglected Role Of Statistical Weighting In Real-time qPCR Data. Andrej-Nikolai
Spiess University Hospital
P086 Bioinformatics workflows with Snakemake Johannes
Köster1,2 1Genome Informatics, Faculty of Medicine,
University P087 Uncertainty
Quantification In Real Time PCR Using Bootstrap Stefan Van Aelst1, Antoon Lievens1, Marc
Van den Bulcke2, Els Goetghebeur1 P088 Analysis of Data from Experimental qPCR
Systems with RKWard Stefan Rödiger1,2,3,
Alexander Böhm1, Jörg Nitschke1, Peter Schierack1,
Werner Lehmann2, Ingolf Schimke3, Christian Schröder1 P089 Advantages
of Cy0 Quantification Using Spline Interpolation Davide Sisti, Michele Guescini, Renato Panebianco, Marco B.L. Rocchi, Vilberto Stocchi Department of Biomolecular Sciences, P090 ChIP-qPCR and qbasePLUS jointly
identify a MYCN-activated miRNA cluster in cancer Barbara D'haene1, Pieter
Mestdagh1,2, Daniel Muth3, Frank Westerman3,
Frank Speleman2, Jo Vandesompele1,2 1Biogazelle, |
Assay
Optimisation, MIQE
and Quality Control
P091 - P105 |
P091 Improving biological
relevancy of transcriptional biomarkers experiments by applying
the MIQE guidelines to pre-clinical and clinical trials Steve
Harakeh1, Maxime Dooms2,
Abalo Chango2, Esam
Azhar1, Philippe Pouillart2, Afif Michel Abdel Nour1,
Flore Depeint2 1KAU/KFRMC/Special Infectious Agents
Unit, Biosafety Level 3, P092 Analytical
and Clinical Validation of In House qPCR for Detection of Human Papillomavirus (HPV) using MIQE Guidelines and ISO 15189 Daniela
Cochicho, Luis Martins, Mário
Cunha, Carmo Ornelas Portuguese
Institute of Oncology P093 Removal of Contaminating DNA from
Commercial qPCR Master Mixes Jorn Remi Henriksen, Elisabeth Lill
Andreassen, Morten Elde P094 Bhaja Krushna Padhi,
Marianela Rosales, Nicholas Huang, Guillaume
Pelletier Hazard
Identification Division, Environmental Health Science and Research Bureau,
HECSB, Health P095 Isabell Pechtl, Marcus Gassman,
Donna McDade Walker, Rüdiger
Salowsky Agilent
Technologies, P096 Development
of a system based on SMART technology for robust transcriptome library
preparation from small quantities of degraded sample Francois-Xavier
Sicot1, Magnolia Bostick2, Cynthia
Chang2, Tommy Duong2, Andrew Farmer2 1Takara Bio Europe, P097 Nucleic acid purification with magnetic
particle processor prior to qPCR Eva Tas,
Marika Suomalainen,
Ossian Saris, Sini Suomalainen Thermo Fisher
Scientific, P098 Reference
Gene Selection And Validation For HT29 And VK2/E6E7 Human Epithelial Cell
Lines Treated With Probiotic And Pathogenic Bacteria Annette Vivi Jacobsen1,2, Bisrat Tewelde1, Jana Jass1,
Nikolai Scherbak1 P099 Patrick Guertler1, Andrea
Harwardt2, Adelina Eicheldinger1, Paul Muschler3,
Ottmar Goerlich1, Ulrich Busch1 1Bavarian Health and Food Safety
Authority, P100 MIQE in
colic microbiota monitoring for
clinical and preclinical trials Maxime Dooms2, Thierry Ribeiro2, Esam Azhar1, Steve Harakeh1, Flore
Depeint2, Afif Michel Abdel Nour1 1KAU/KFRMC/Special Infectious Agents
Unit, Biosafety Level 3, P101 Isabell Pechtl, Melissa Liu, Maria Celeste Ramirez, Rüdiger Salowsky Agilent
Technologies, P102 High Throughput Miniaturized PCR using the
Echo® 525 Liquid Handler Celeste
Glazer, Carl Jarman, Brent Eaton P103 Quality control of RNA using the Agilent
2200 TapeStation system Isabell Pechtl, Arunkumar Padmanaban, Rüdiger Salowsky, Charmian Agilent
Technologies, P104 Quantification of GMO at a Level of
0.1 % – A Statistical Approach Using Frequency Distribution Lars Gerdes, Ulrich Busch, Sven Pecoraro Bavarian Health and
Food Safety Authority (LGL), P105 Gene Expression Profiling: qPCR Toolkit
for Quality Control David
Svec1, Jenny Jacobsson1, Robert
Sjöback1, Mikael Kubista1,2 1TATAA |