& NGS 2015 Talk and Poster Sessions
of "BDQ Young Scientist Poster Awards"
The new open access journal Biomolecular Detection and Quantification
(BDQ) is proud to sponsor three "BDQ Young Scientist Poster Awards" at
the qPCR & NGS 2015 event.
The posters will be evaluated by an international jury on the basis of
originality of the approach; quality of the work (e.g. appropriate
methodology, interpretation of results, conclusiveness); quality of the
presentation (e.g. clarity, response to questions); and self-reliance
and independence (not one of co-authors, but the most active, if more
than one author).
Eligible for the BDQ Young
Scientist Poster Award: The contest is
open to students and early career scientists.
Presentation: BDQ Editor-in-Chief
Stephen Bustin will award the three poster prizes
on Wednesday afternoon directly after the lunch break March 25, 2015.
- BDQ Young Scientist Poster Award - First Prize
The winner is awarded 700 Euros and a free submission to BDQ
- BDQ Young Scientist Poster Award – Second Prize
The winner is awarded 200 Euros and a free submission to BDQ
- BDQ Young Scientist Poster Award – Third Prize
The winner is awarded 100 Euros and a free submission to BDQ
Authors will be able to express their interest in participating in the
BDQ Young Scientist Poster Award contest when submitting their
abstract. Note that authors are only able to participate if their paper
has been selected as a poster by the conference organizer.
register and submit your abstract using the Internet based ConfTool
registration and submission
platform => Registration.qPCR-NGS-2015.net
the conference agenda
-- HTML version AgendaHTML.qPCR-NGS-2015.net
and PDF version AgendaPDF.qPCR-NGS-2015.net
- PCR in less than
30 seconds: Efficient, specific amplification with increased primer and
Carl T Wittwer; University of Utah, United States of America
- Digital PLA
(Proximity Ligation Assay)
Stephen Bustin; Anglia Ruskin University, Chelmsford, Essex, United
Gary Schroth; Illumina, United States of America
- How could digital
PCR benefit clinical analysis?
Jim Francis Huggett; LGC, United Kingdom
- Updated evaluation of
quantitative miRNA expression platforms in the microRNA quality control
Jo Vandesompele; Ghent
University on behalf of the microRNA quality control study consortium,
- Mass Spectrometry
Based Draft Of The Human Proteome.
Bernhard Küster; TU München, Freising Weihenstephan, Germany
- Next Generation
Sequencing Applications to the study of human and animal Microbiomes.
Karen E. Nelson, J. Craig Venter Institute, United States of America
Profiling of Circulating Tumor Cells: a Prognostic and Predictive
Biomarker in Cancer.
Mikael Kubista; TATAA Biocenter, Sweden
- Variability of
the Reverse Transcription Step: Practical Implications.
Tania Nolan; The Gene Team, United Kingdom
- Decoding lncRNA
functions using high-throughput pathway perturbation.
Pieter Mestdagh; Biogazelle, Belgium
& NGS 2015 Talk and Poster Sessions
topic: Advanced Molecular Diagnostics
This session is focusing on the application of highly
sophisticated methods, application and algorithms to discover, detect,
and validate molecular diagnostic markers. A special focus is lying on
the integrative analysis of multi level biomarkers, e.g. microRNA –
mRNA, integrative analysis of genomic, proteomic, metabolomic and
topic: Biomarker Discovery
A session about the discovery, identification and
molecular biomarkers: diagnostic, prognostic or therapeutic
DNA, RNA, microRNA or small RNA, metabolome and proteome level (e.g.
disease markers, cancer or stem-cells markers, tissue specific markers,
differentiation markers, methylation markers). New assay systems will
be presented like high throughput proteomics (MS based proteome map),
PLA (Proximity Ligation Assay) or PEA (Proximity Elongation Assay).
topic: Next Generation Sequencing (NGS)
NGS applications offer new holistic analysis of any
nucleic acid, to investigate the Genome, Exome, Epigenome,
Transcriptome and Splicome (total RNA, microRNA, small RNA Seq, long
ncRNA), CHIP purified nucleic acids, etc.
Various NGS sub-sessions will be presented:
• NGS overview
talks - information technology in the era of NGS
• Pre NGS -
sample prep & setup & library generation
• NGS – new
sequencing technologies (e.g. single molecule and pore sequencing)
• NGS – data
analysis (data management, mapping, alignment algorithms, data de novo
• NGS –
& Single-cells diagnostics
Focus is on micro-genomics, the application of molecular
to detect biomarkers in a minimal amount of tissue or matrix. New
applications in single-cell isolation, separation or characterisation
technologies, laser micro dissection, isolating circulating tumour
cells (CTC), pre-amplification techniques, sub-cellular PCR,
micro-manipulation of cell clusters, cellular micro injection, ,
single-cell handling, FACS sorting and spotting.
A new class of biomarkers are circulating nucleic acids
RNA, small RNAs, and long ncRNAs) which are free floating (?), bound to
proteins or covered in micro-vesicles. One focus will be on exosome or
micro-vesicles isolation, purification for bio-liquids, amount and size
quantification, and characterisation techniques, including
intra-cellular and membrane bound protein or lipid markers.
Diagnostics in Agriculture, Veterinary Medicine, Food &
This session is dedicated to molecular diagnostics in
Science, with focus on Agriculture, Veterinary Medicine, and
Environmental Science (which is the major research focus here at the
TUM campus in Weihenstephan). All applications of highly sophisticated
quantification methods, field application or algorithms to research the
wide field of agro-veterinarian life science are very welcome.
& QM & Standardisation strategies in molecular diagnostics
This session is focusing on standardisation strategies
quality management in molecular diagnostics. The goal is to guarantee
better and more valid results. Of special interest in the context of
qPCR are the MIQE guidelines (minimum information for publication of
quantitative real-time PCR experiments). Following these guidelines
will encourage better experimental practice, allowing more reliable and
unequivocal interpretation of qPCR results.
PCR & Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and
amplify nucleic acids including DNA, cDNA, mRNA or microRNA. It allows
a more reliable collection and highly sensitive measurement of nucleic
acid amounts, applications in copy number variants, point mutations in
RNAs -- microRNA, small RNAs, long non-coding RNAs
This session is dedicated to the family of non-coding
its RNAi mechanism and applications: extraction of non-coding
RT-qPCR technologies to detect microRNA, long-non coding RNAs and new
classes of small RNAs, siRNA knock down applications, microRNA targets
and microRNA precursors, new siRNA manipulation, etc.
Data Analysis -- BioStatistics & BioInformatics
The data analysis in RT-qPCR expression profiling
still challenging and time consuming. From the literature it is well
known, that a lot of error is introduced by wrong experimental study
design, reference gene normalisation or qPCR data analysis. This
session is focusing on useful algorithms and software applications for
data mining, calculation of relative expression, primer and probe
design for any kind of nucleic acid (mRNA, microRNA, piRNA), real-time
PCR efficiency determination, mathematical modelling, multivariate
expression profiling raw data analysis, statistics in real-time PCR,
data management, multi-way expression profiling, multiple regression
analysis, 3D data visualization, and much more!
- Agilent Technologies -- 2 talks
- Life Technologies -- 2 talks
to our scientific organisation
headed by Michael
W. Pfaffl qPCR-NGSfirstname.lastname@example.org
2014 - 2015