Confirmed Speakers & preliminary Talk Titles

Symposium Talk and Poster sessions:

Please register and submit your abstracts (talk or poster) using the Internet based ConfTool registration and submission platform => Registration.qPCR-NGS-2015.net


Publish your conference contribution as a 'Full Paper' in "Biomolecular Detection and Quantification"

Biomolecular Detection and Quantification (BDQ) is an open access, peer-reviewed international journal dedicated to championing excellence in molecular study design, measurement, data analysis and reporting. Its focus is on the application of qualitative and quantitative molecular methodologies to all areas of clinical and life sciences.

A special BDQ issue with the title “Advanced Molecular Diagnostics for Biomarker Discovery” will be published in summer 2105. You can submit your conference contribution (talk or poster) manuscript until 30th April 2015.

Please submit your manuscript here => http://ees.elsevier.com/bdq/default.asp?pg=preRegistration.asp
After REGISTRATION and LOGIN select  "Special Issue (SI)  Adv. Molecular Diagnostics"

Download recent BDQ papers on Elsevier Science Direct.





Main topic:   Advanced Molecular Diagnostics


Speakers papers
CARL WITTWER
Professor, Department of Pathology, University of Utah, US
Adjunct Professor, Biomedical Engineering, University of Utah, US

KEYNOTE LECTURE:
PCR in less than 30 seconds:  Efficient, specific amplification with increased primer and polymerase.

STEPHEN A. BUSTIN
Professor of Molecular Medicine, Postgraduate Medical Institute, Faculty of Health, Social Care & Education, Anglia Ruskin University, UK

KEYNOTE LECTURE:
Digital PLA  (Proximity Ligation Assay)
LARS ULLERICH
R&D, GNA Biosolutions GmbH, Planegg / Martinsried, Germany

Laser driven PCR for ultrafast DNA detection.
JO STANTON
PhD, Department of Anatomy, University of Otago, New Zealand


Mobile applications of qPCR.



Main topic:   Biomarker Discovery



Protein Biomarkers:



BERNHARD KÜSTER
Chair of Proteomics and Bioanalytics
member of the Excellence Cluster CIPSM (Centre for Integrated Protein Science Munich),  TUM Weihenstephan, Freising, Germany

KEYNOTE LECTURE:
Mass-spectrometry-based draft of the human proteome.
GEMMA JOHNSON
Postgraduate Medical Institute, Faculty of Health, Social Care & Education, Anglia Ruskin University, UK

Proximity Ligation assay for the early, specific detection of infectious aspergillosis.
Nucleic Acid Biomarkers & Circulating NAs:



MERCEDES TKACH
INSERM U932, Institut Curie, PI Team "Exosomes and Tumor Growth", Paris, France

Biology of exosomes and other extracellular vesicles, new potential circulating biomarkers.

STANISLAS WERFEL
Institute of Pharmacology and Toxicology
Technische Universität München (TUM), Munich, Germany

CircRNA: a new class of non-coding ribonucleic acid molecules.
MICHAEL W. PFAFFL
Prof. of Molecular Physiology, Chair of Physiology, TUM Weihenstephan, Germany

Expression profiling of Exosomes.
ROBERT LOEWE
CEO, GeneWake GmbH, Neuried,Germany

miRNA profiling in tumor tissue, body fluids and exosomes - a combinational techniques approach of NGS and qPCR.

CONSTANZE KINDLER & JONATHAN SHAFFER
QIAGEN R&D, Hilden, Germany


Exosomes – A novel approach to biomarker identification.



Main topic:   Next Generation Sequencing (NGS)
Various NGS sub-sessions will be presented:
  • NGS overview talks - information technology in the era of NGS
  • Pre NGS - sample prep & setup & library generation
  • NGS – new sequencing technologies (e.g. single molecule, pore sequencing, etc. )
  • NGS – data analysis     (data management, mapping, alignment algorithms, data de novo assembly, etc. )
  • NGS – diagnostic applications

KAREN NELSON
Professor, President of J. Craig Venter Institute, San Diego, USA

KEYNOTE LECTURE:
Next Generation Sequencing Applications to the study of human and animal microbiomes.

GARY SCHROTH
PhD, Distinguished Scientist, Product Development, Illumina, San Diego, CA


KEYNOTE LECTURE:
Advanced Topics in RNA-Seq - Cancer, FFPE, microbiology and viruses?

IAIN C. MACAULAY
PhD, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

G&T-Seq: Combined DNA and RNA sequencing from a single cell.

FANG CHEN
PhD, Scientist at BGI-Shenzhen, Yantian District, Shenzhen, People's Republic of China

New advances in sequencing-based technology in Clinical Applcation and Molecular Diagnosis.

BURKHARD ROLF
PD PhD, Director DNA Forensic Services, Eurofins Medigenomix Forensik GmbH

Differentiation of identical twins by Next Generation Sequencing.
JUSTIN O’GRADY
Lecturer in Medical Microbiology, Norwich Medical School, University of East Anglia, Norwich, UK

Pathogen DNA enrichment strategies for NGS based clinical diagnostics.
VICENT PELECHANO
PhD, Staff Scientist,  Steinmetz  Group,  Genome Biology Unit,
EMBL, Heidelberg, Germany


Inferring ribosome dynamics from mRNA degradome sequencing.
MICHAEL RHODES
PhD, Dir. Applications Development, NanoString Technologies, Seattle, WA 98109, United States

Nanostring - The next step after NGS.
ALEXANDER SCHRAMM
Senior Scientist, Universitätsklinikum Essen, Kinderklinik, Onkologisches Labor, Essen, Germany

Integrative analyses of primary and relapse tumor samples identifies genomic changes during cancer progression.
THOMAS CLAVEL
Junior Research Group Intestinal Microbiome
ZIEL Research Center for Nutrition and Food Sciences, TU-München, Freising, Germany

Microbiomes via sequencing: challenges and opportunities.
SIMON RAYNER
PhD., Scientific Manager NGS Bioinformatics, Exiqon, Denmark

Fast and flexible bioinformatics analysis of RNA seq data to provide biological insight.

MARKUS SCHMITT
PhD, Scientific Sales Representative,
GATC Biotech AG, Konstanz, Germany

Whole Exome Sequencing for Detection of Genetic Variants to Predict Chronic Lung Allograft Dysfunction.
STEVE LEFEVER
UGent, pxlence, Center for Medical Genetics Ghent, Ghent University, Ghent, Belgium

Targeted resequencing and variant validation using pxlence PCR assays.
MATTHIAS SCHERF & MARTIN SEIFERT
CTO & CSO, Genomatix Software GmbH, Munich, Germany

Chances and challenges in RNA-Seq analysis.
JAN HELLEMANS
Biogazelle, Technologiepark 3, B-9052 Zwijnaarde, Belgium

Benchmarking of RNA-seq data processing pipelines using whole transcriptome qPCR expression data.
ANDREAS DAHL
PhD, Leader Deep Sequencing-Group of SFB 655, TU Dresden, Germany


From a ton of RNA to a single cell - Challenges for a Sequencing core lab to achieve robust Trancriptome Profiling.

MICHAEL WITTIG
Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Germany

High resolution NGS-based HLA-typing using in-solution targeted enrichment.




MicroGenomics & Single-cells diagnostics



MIKAEL KUBISTA
Prof. of  Biotechnology, BTU, Czech Academy of Sciences & TATAA Biocenter, Sweden


KEYNOTE LECTURE:
High throughput Expression Profiling of Circulating Tumor Cells.
STEPHAN LORENZ
Senior Scientific Manager, Wellcome Trust Sanger Institute, Single Cell Genomics Core Facility, Hinxton, Cambridge, United Kingdom
Walkaway Automated Single Cell Genomics.
BIRGIT LISS
Director, Institute of Applied Physiology, University of Ulm, Germany

Analyzing function and gene expression of individual neurons in health and disease: from mice to men.
MICHAEL MANCINI
Professor & Academic Director, Integrated Microscopy Core & Co-Director, John S. Dunn GCC for Chemical Genomics
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA
Interrogating nuclear receptor-mediated gene regulation by single cell analysis:  High throughput antibody/mRNA approaches for multiOmics analysis.
ANDERS STAHLBERG
Prof. and Senior Scientist, Sahlgrenska Cancer Center, University of Gothenburg, Sweden


Multi-analyte analysis at single-cell level in Myxoid Liposarcoma reveals previously unknown cell states.
MARK KIBSCHULL
Post Doctoral Research Fellow, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada
From Cultures to Results in One Day:  A New Workflow for Accurate and Reproducible Large Scale qPCR Expression Analysis from Limited Samples in Stem Cell Research.
RADEK SINDELKA
PhD, Insitute of  Biotechnology, BTU, Czech Academy of Sciences, CZ

Intracellular molecular gradients prepare oocytes for asymmetric cell division.



Molecular Diagnostics in Agriculture, Veterinary Medicine, Food and Environmental Science



PATRICE MARTIN & CLAUDIA BEVILACQUA
INRA (Institut National de la Recherche Agronomique), Genetique animale et Biologie integrative, Jouy-en-Josas, France

Comparison of five different RNAsources to examine the lactating bovine mammary gland transcriptome using RNA-Sequencing.
ILKA HAASE
Analytical Service Manager, DNA Food Testing, Applied Genomics, Eurofins Genomics, Germany
DNA-based Food Authenticity and Safety Control using Advanced Barcoding by Next Generation Sequencing.


IRMGARD RIEDMAIER-SPRENZEL
PhD, Senior Scientist, Chair of Physiology, TUM Weihenstephan, Germany

Transcriptomic Biomarkers in Food Safety.

MOJCA MILAVEC
PhD, National Institute of Biology (NIB), Ljubljana, Slovenia

Challenges in analysis of genetically modified organisms.
MAREIKE WENNING
PhD, Department of Microbiology at the Central Institute for Food and Nutrition Research (ZIEL), TUM Weihenstephan, Germany


Optimized library preparation for sequencing of whole genomes and low density microbiota by Illumina-based NGS.
MARC YOUNG
Unit of Molecular Zoology, Chair of Zoology, Department of Animal Science, Technische Universität München, Freising, Germany

A transcriptomic approach to ascertain insights into the etiology of Brown Trout Syndrome.



MIQE & QM & Standardisation strategies in molecular diagnostics



JO VANDESOMPELE
Center for Medical Genetics Ghent, Ghent University, Ghent, Belgium
Biogazelle, Technologiepark 3, B-9052 Zwijnaarde, Belgium

KEYNOTE LECTURE:
Assessing microRNA gene expression platform performance in the miRQC study.
TANIA NOLAN
Institute of Population Health, Faculty of Medical and Human Sciences, University of Manchester, UK

KEYNOTE LECTURE:
Variability of the reverse transcription step: practical implications.
MATJAZ HREN
Biosistemika, Research & Development LLC, Tehnoloski park, Ljubljana, Slovenia


Reducing time and error rates by automating qPCR workflows.
ATHANASIA SPANDIDOS
1st Department of Pathology, National and Kapodistrian University of Athens, Athens, Greece
Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, US

PrimerBank: a Database of Human and Mouse PCR Primer Pairs for Gene Expression, Detection and Quantification.
DAVE SCHUSTER
R & D, Quanta BioSciences, Perry Parkway, Gaithersburg, MD, USA

qScript XLT cDNA SuperMix: overcoming the common pitfalls of cDNA synthesis as applied to relative quantification and RT-qPCR.
JENS BJÖRKMAN
Senior Scientist, TATAA Biocenter, Sweden


New tools for quality assessment of RNA in Molecular Diagnostics.

AFIF M. ABDEL NOUR
PhD, Field Application Specialist, Middle East, Turkey, Africa, Bio-Rad LSG, Buhaira Chorniche, Sharjah, U.A.E

Digital publication: how making the MIQE guidelines easier to follow.
SCOTT ROSE
PhD, Director of Enzyme Development, Integrated DNA Technologies, Coralville, IA, USA

Customized Solution Hybridization Enrichment Panels: From A Single Gene To An Entire Exome- Design, Usage, And How To Increase Your Odds For A Successful Outcome.

ALEXANDRA OVER
Biosearch Technologies,
Waltham, MA, USA

ThermaStop: Properties and Benefits.



Digital PCR  &  Nano-fluidics



JIM HUGGETT
Principal Scientist, Nucleic Acid Research, Molecular and Cell Biology, Science and Innovation
LGC, Teddington, Middlesex, UK

KEYNOTE LECTURE:
Sources of error when performing absolute quantification using digital PCR.
SVILEN TZONEV
Staff Scientist - Digital Biology Center, Bio-Rad Laboratories, USA
Rapid and Ultra-Sensitive Single-Cell Transcript Profiling with Droplet Digital PCR (ddPCRTM): Applications for neuronal differentiation.
TIM FORSHEW
UCL Group Leader - Translational Cancer Genetics, UCL Cancer Institute, London, UK

NGS and dPCR analysis of circulating tumour DNA and their potential uses in cancer patient care.
ADAM CORNER
PhD, RainDance Technologies, USA Headquarters, Middlesex Turnpike Billerica, MA, USA
RainDrop™ Digital PCR System Single Molecule Counting: making the most of 10 million droplets.



Non-coding RNAs -- microRNA, small RNAs, long non-coding RNAs



PIETER MESTDAGH
Biogazelle, Technologiepark 3, B-9052 Zwijnaarde, Belgium

KEYNOTE LECTURE:
Decoding lncRNA functions using high-throughput pathway perturbation.
ROBERT SJÖBACK
Senior Scientist, TATAA Biocenter, Sweden


New, sensitive and specific method for quantitative analysis of microRNA.

SIMON BAKER
Ph.D., Global Director of Research & Development, Bioline, UK
Visiting Research Fellow at Birkbeck College, University of London, UK
miRNA profiling in Cancer, Stem Cells and Body Fluids.
DAVID SIMPSON
Senior Lecturer, Queen's University Belfast, Centre for Experimental Medicine, Institute of Clinical Science, Royal Victoria Hospital, Belfast, Northern Ireland


Development and optimisation of PCR assays to analyse microRNAs and their target genes.



qPCR Data Analysis -- BioStatistics & BioInformatics



PHILIP ZIMMERMANN
CEO NEBION AG, Zürich, Switzerland


Beyond data analysis - interpreting your results in light of biological contexts.
ANDREAS UNTERGASSER
University of Heidelberg, Heidelberg, Germany


RDML qPCR Data Format - Ready For The Next Level?
ANNE ARENS
QIAGEN, Advanced Genomics, Hilden, Germany


NGS/Genomics from “sample to insight” - analysis, visualization and interpretation of NGS data.
JAN RUIJTER
Senior Scientist, Academic Medical Center, University Amsterdam, The Netherlands


Removal of Between-Plate Variation in qPCR with Factor Correction: Completion of the Analysis Pipeline Supported by RDML.




Technical Lunch-Time Seminars



ROBERT LOEWE
CEO, GeneWake GmbH, Neuried,Germany
High resolution melting – how to produce highly reproducible measurements.
BERND MARTIN
Agilent Technologies, Braunschweig, Germany

Total Confidence qPCR – The New AriaMX Real-Time PCR System.
ROMERO ATOCHA
Liquid Biopsy Laboratory, Hospital Universitario Puerta de Hierro, Madrid Spain

Digital PCR analysis of tumor-specific mutations from peripheral blood in ER positive breast cancer samples.
ULRICH CERTA
Professor, R&D, Roche, Basel


Interrogating the human transcriptome for biomarker discovery- from the ‘ome to routine qPCR.


Please direct your enquiry to our scientific organisation team, headed by Michael W. Pfaffl  qPCR-NGS-2015@wzw.tum.de
©  2014 - 2015