2nd International qPCR Symposium &
Workshop & qPCR Matrix Workshop
The whole story of
quantitative PCR – from Tissue Preparation to BioInformatics
5 - 9th September 2005 in Freising-Weihenstephan,
Technical University of Munich,
Physiology Weihenstephan, Germany
behalf of the Organisation Committee and the Scientific Board it is
great pleasure to invite you to the 2nd International qPCR
Symposium & Application Workshop to be held September 5-9,
2005, at the Center of Life Science in Freising Weihenstephan,
Technische Universität München (Germany). The
interest in the qPCR 2004 - 1st International qPCR Symposium &
Application Workshop by 465 participants from 44 countries and 32
international companies in
the qPCR Industrial Exhibition led us to the decision to repeat it in
event will focus on all aspects of qPCR technology and its applications
in research and diagnostics. Leading academic researchers and
industrial contributors in the field will be participate in the
symposium, which will be an arena for fruitful discussions between
researchers of different backgrounds. Title
of the Symposium talks. The Symposium, the Industrial
Exhibition and two types of associated Workshops offer an overview of
the present knowledge and future developments in qPCR technology and
its wide applications. The subtitle of the qPCR 2005
The whole story of quantitative PCR – from
Tissue Preparation to BioInformatics
The event is divided in
qPCR Symposium from 5 – 7th September,
is the central lecture hall and the foyer at TUM (Technical University
Munich) in Freising Weihenstephan. We are part of the biggest Biotech
Cluster in Germany and Europe, located close to the Munich airport, and
in the heart of Bavaria.
qPCR Industrial Exhibition from
5 – 7th September
qPCR Workshop from 7 – 9th September ( TATAA application workshop and the qPCR Matrix Workshop )
The scientific organization will be managed by a similar
team like last year:
I am looking
forward meeting you in September at the Symposium in
of Molecular Science QM, School of Medicine, London, UK
of Biotechnology, MultiD Analyses AB, Gothenburg, Sweden
Genomics Core Facility, Heidelberg, Germany
of TATAA Biocenter, Sweden
(coordinator TATAA application workshop)
|Heinrich H.D. Meyer
of Physiology, Weihenstephan, Germany
|Michael W. Pfaffl
scientific coordinator of the Symposium and the Matrix Workshop qPCR2005@wzw.tum.de
|Russell G. Higuchi
in real-time PCR, Associate Director of the Human Genetics Department,
Roche Molecular Systems, Alemada, CA, USA
Wild, TUM-Tech GmbH Munich firstname.lastname@example.org
NEWS - qPCR 2005
- NEWS - qPCR 2005 - NEWS
- qPCR 2005 - NEWS
the TATAA qPCR
application workshop is fully booked by 20 people ! (
22nd July 2005 )
please register and participate in the qPCR Matrix Workshop
is fully booked by 60 people ! (
31th August 2005 )
organisation of the qPCR Matrix Workshop on Monday late afternoon (
17:00 - 18:30 ) => overview
interesting sessions will be presented in the qPCR Matrix
Workshopov from Wednesday till Friday,
least 8 different real-time cyclers ( new Roche
LightCycler 480, new Eppendorf RealPlex, new Bio-Rad
iQ5 Cycler, Rotor-Gene
3000 by Corbett Research, Applied Biosystems 7900,
Stratagene MX cycler(s), SmartCycler, etc. )
of high-throughput technologies using pipetting- and nucleic
acid extraction robots (by Eppendorf, Applied
Biosystems, and Corbett Research),
of new real-time applications ( SYBR Green, fast-PCR,
multiplex-qPCR, one-step and two-step qRT-PCR systems, low density
arrays, universal probe library, genotyping, etc. ) will be presented
Roche, Invitrogen, Promega, etc.
of new BioInformatics tools for relative quantification and
statistical analysis of
real-time data, by Jo Vandesomplete, Jan Hellemans, Mikael Kubista,
Michael W. Pfaffl and co-workers,
We welcome our
international speakers and symposium participants at the qPCR 2005
official conference and workshop language is ENGLISH. No simultaneous
translation will be provided.
All oral presentation, poster and abstarcts must be presented in
scientific contributions will be published in the qPCR
Symposium Proceedings ISBN 3000166874
|new deadline =>
31st July 2005 (submission
based abstract submission platform =>
indicate if you want to present either a Poster or give an oral
Presentation via MS PowerPoint.
Spring 2006 the PDF download of the contributions is password
protected and only accessible for Symposium and Workshop
abstract should be a
one-page document based on unpublished material and written in good
standard English. The text should clearly state objectives,
methodology, results, and conclusion. Simple tables and graphs are
- Poster will be DIN A0 (portrait format :120
high x 90 cm/35 inch wide)
of every communication at the Conference is subjected to formal
registration of at least one co-author.
based registration platform =>
registration fee includes:
printed proceedings ISBN 3000166874
containing all the poster and talk abstracts.
access: all presentation
slides and posters will be shown online on the symposium homepage, in
a password protected area.
catering service at the Symposium:
includes all kind of drinks (coffee, tea, alcoholic, and
snacks, three lunch meals (Mo. - Wed.) and two events: a Poster-Party on Monday evening with live
music (sponsored by and Roche Aplied ScienceEppendorf)
and a downtown Evening-Event at
Tuesday in Freising with a variety of delicious buffets. A
detailed qPCR 2005 welfare guide will be published soon !
catering service at the Workshops:
includes all kind of drinks (coffee, tea, and non-alcoholic),
snacks, and three lunch meals (Wed. - Fri.).
symposium proceedings ISBN
3000166874 including all Abstract,
Posters and Oral Presentations. Proceedings will be published as
FULL PDF PRESENTATIONS on the qPCR2005.Gene-Quantification.info
web page. As wanted by the authors the PDF download of the
contributions is password protected and only accessible for Symposium
or Application Workshop participants, until spring 2006.
symposium will focus on approximately 35 lectures presented by
internationally recognised experts in their field. The emphasis will be
on unbiased, didactic information exchange. Internationally
renown speakers will be participating in a lively and exciting
programme enabling the valuable exchange of information in the qPCR
third of the talks will be presented by selected invited speakers,
third will be selected from the submitted abstracts and one
third will be presented by qPCR company R&D
representatives. All scientific contributions will be published
in the qPCR 2005 Symposium Proceedings.
The sessions will be grouped like the work flow in a scientific lab -
from RNA/DNA tissue preparation to qPCR BioInformatics applications.
Starting with sampling, NA isolation, RT, qPCR, CP determination and
how to deal with the generated results. New methods of
quantification strategies, normalization procedures, new calculation
algorithms and exact nucleic acid quantification in combination
with array technology will be presented. Depending on the submitted
abstract we want to have a GMO and Food analysis session.
of the Symposium talks
Symposium and Poster sessions:
|sampling technologies, DNA / RNA
purification, DNA / RNA quality control, Reverse Transcription,
efficiency of DNA / RNA extraction, influence on
of the real-time reaction, inhibition of negative effects, new dyes, increase real-time PCR
efficiency, optimisation of multiplexing, etc........
standards, comparability between runs, tests, labs and countries,
national and international studies on qPCR standardisation, etc.......
normalisation, one housekeeping gene vs. multiple housekeeping
relative quantification software applications, etc........
profiling, comparability of cDNA array results with
real-time RT-PCR, comparability of real-time RT-PCR with other methods,
|new quantification methods, high
throughput applications, CHIP technology, multiplexing, new dyes or
probe technologies, new quantification strategies, etc.......
|GMO Analytics & Food Hygiene
|GMO problematic, food testing, microbiology, validation of
methods, special plant and food extraction methods, etc.....
|new software applications,
calculation of relative expression, exact determine of real-time PCR
efficiency, optimal CP determination, mathematical algorithms, data
management, statistics in real-time PCR, 3D data visualisation,
We plan to perform two types of workshop.
One will be like a classical qPCR Application
Workshop (type 1) organized by the TATAA Biocenter
in Sweden (www.tataa.com), and the
other is a new type of Matrix Workshop (type
2) where people rotate and can visit 6 different workshop
sessions. Both qPCR Workshop typess will be held from afternoon
7th till late afternoon 9th September 2005 and will be divided
in two different workshop types. The students can
visit either the type 1 or the type 2.
and qPCR companies will be responsible
for their own equipment: all hardware, software, kits
and disposables. The organizers at the TUM-Weihenstephan
can provide four different rooms (P1, P2, P3 and P4) with
different number of benches (from 7 to 17 benches, each fitting 4-6
students 1-2 and workshop session instructors), chairs, electricity,
W-LAN, fridges, freezers, crushed ice, water, and…...
held on two lab-benches, one for sample preparation and one for the
classical qPCR workshop, presented by TATAA
room number 2 - Plan of room P2
workshop offers hands-on training by leading experts
in qPCR from TATAA Biocenter. Major probe technologies
will be demonstrated, leading and emerging instrument platforms will be
presented, and advanced primer and probe design programs will be
available. Hands-on training will be provided on quantitative gene
expression, including design and optimization of both RT and qPCR
protocols. Participating companies are asked to support the workshop
with cyclers and kits. To support of the with PCR cyclers, qPCR kits,
consumables, centrifuges or pipettes please contact Neven Zoric at the
TATAA Biocenter Neven.Zoric@tataa.com
NEW type of
qPCR Matrix Workshop, presented by
the qPCR companies itself
number 1, 3 and 4 => Plans of P1 and P3 and
New type of interactive
Researchers and qPCR companies are addressed to present their
technologies (cycler, chemistry, kits or software applications) by
their own product specialists.
Each student can
visit 6 different session types at the 6 time frames during the qPCR
workshop. After finishing a session the students move to the next bench
and session type.
In each time frame 4-6 students can be educated by one or two
instructors in a defined subject.
Each session can use two benches, one for sample preparation and one
for the qPCR applications.
Each workshop session will last for 3 hours.
Three sessions will be presented at one day.
The students can choose and book the sessions on
Monday afternoon (17:00 - 18:30) registartion desk.
The session instructors from the companies are asked to generate
interesting sessions in which the student can learn about one distinct
application or can solve one special problem. For session ideas
arrangements please contact M. W. Pfaffl qPCR2005@wzw.tum.de
qPCR Industrial Exhibition
industrial exhibition will be held during the qPCR Symposium from 5
– 7th September in the foyer of the central lecture hall complex
(green frame) and in two side rooms S1 and S2 (blue frame).
According to the booth location we have 26 booth in different
prize categories. They are all grouped around the central lecture halls
H14 and H15
and fits up to 650 people.
Parallel to the Symposium an industrial Exhibition will take place
along with the congress, where an intensive conversation with numerous
companies is possible (real-time PCR Cyclers, Kit
Producers, Nucleic Acid Purification Systems, Enzyme Producers,
Plasticware Suppliers, etc.)
=> Link to the Industrial
To book a
booth and for further details about the industrial exhibition, e.g.
exhibition contract, technical management, stand allocation, electronic
installations, internet connection (W-LAN), etc. we are pleased to
answer your questions.
Please contact Dr. Ulrich Wild from TUM-Tech GmbH Munich email@example.com