qPCR Matrix Workshop
Two types of workshop will be perfomed at the qPCR 2005 Event:
One will be like a classical qPCR Application Workshop organized by the TATAA Biocenter in Sweden (www.tataa.com), and the other is a new type of qPCR Matrix Workshop where people rotate and can visit 6 different workshop sessions. Both qPCR Workshop typess will be held from afternoon 7th till late afternoon 9th September 2005 and will be divided in two different workshop types. The students can visit either the   TATAA Workshop   or   the qPCR Matrix Workshop.
qPCR Matrix Workshop

A total new type of interactive workshop.  Practical room number 1, 3 and 4 =>  Plans of  P1  and  P3  and  P4
  • Researchers and qPCR companies are addressed to present their technologies (cycler, chemistry, kits or software applications) by their own product application specialists.
  • Each student can visit 6 different session types in 6 time frames (time management see below).
  • After finishing a session the students move to the next bench and session type.
  • In each time session and frame 4-6 students can be educated by 1-2 session instructors.
  • Each session will be held on two lab-benches, one for sample preparation and one for the qPCR applications.
  • Each workshop session will last for 3 hours.
  • Maximal three sessions will be presented at one day.
  • The students can choose and book the sessions at the meeting registartion desk ( Mo. 5th Sept. 17:00 - 18:30 ).
Announced Sessions
ID
 Type / Room
 Session Instructor
Institution / Company
 Session title
and description
1
 Application
P1
 Andrés Jarrin
Application specialist, Eppendorf AG, Hamburg, Germany
 Reaction setup and quantitative PCR analysis with the Eppendorf epMotion liquid handling workstation and Mastercycler realplex.
2
 Application
P1
 Cornelia Goldstein
Irmgard Obermaier
Roche Applied Science, Mannheim, Germany
 Accurate Gene Expression Analysis with High Flexibility: LightCycler® 480 System and Universal ProbeLibrary.
Anmeldeformular  ( Deutsch / German )
Registration form  ( English )
3
 Application
P1
 Martin Schlumpberger
Ina Scheuerpflug
QIAGEN GmbH, R&D, Hilden, Germany
 Easy Isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections and SYBR Green qPCR with new, ready-to-use QuantiTect Primer Assays.   QIAGEN_Workshop_Easy_Isolation_of_RNA_Details.pdf
Application
P1
 Dirk Loeffert
Annette Tietze
QIAGEN GmbH, R&D, Hilden, Germany
 		Fast and efficient: Synthesis of cDNA without RNA isolation and reliable real-time multiplex PCR.
QIAGEN_Workshop_Fast_and_Efficient_Details.pdf
4
 Application
P1
 Thomas Kaiser
Rudolf Walser
LTF-Labotechnik, Germany
Corbett Research, Australia		 Primer & probe matrix optimization and PCR setup using the CAS-1200 and high speed runs on the Rotor-Gene.
5
 Application
P1
 Steffen Müller
Stratagene, Field Application Scientist
 FastPCR - Speed up results with FullVelocity.
6
 Application
P1
 Marcus Neusser
Gene Expression Devision, Bio-Rad Laboratories
 Fast PCR Applications: Sense and Nonsense
Success with SYBR - How to Design and Implement High-Quality SYBR Green I  Reactions.
7
 Application
P4
 Kyle Hooper &
Alyssa TenHarmsel
Promega Corporation, Woods Hollow Road, Madison, WI, USA		 Simplify multiplexed qPCR analysis a new approach with the Plexor Real-Time PCR System.
8
 Application
P4
 Astrid Potratz
Support Specialist, Applied Biosystems, Germany		 From Tissue to a Gene Expression Profile in less than 5 hours.
Using the Applied Biosystems 7900 Real-time PCR System
 in combination with the ABI Prism 6100 Nucleic Acid PrepStation.
9
 Application
P4
 Andreas Eckelt
Cepheid SA, Odenthal, Germany

 Efficient and fast transition of a gel-based to a real time PCR reaction.
Content: a standard PCR reaction will be converted to a  real-time PCR assay. Temperatur profile, buffer conditions and primer concentration will be adopted.
10
 Application
P4
 Mark Andersen
R&D Scientist, Invitrogen, USA
 Analyze your SNP genotype and buccal cell expression profile with CellsDirect(TM) and LUX(TM) detection technology.
11
 BioInformatics
P3
 Michael W. Pfaffl
and collegues
Physiology Weihenstephan
Center of Life Science, Technical University of Munich, Germany
 Application of various new REST software versions:
Relative Expression Software Tool
REST-384   (REST high troughput)
REST-MCS  (REST with Multiple Condition Solver)
REST-RG  (REST for Rotor-Gene)
REST-2005    (REST stand alone application)      Time frame:  1 and 5
BioInformatics
P3
 Jo Vandesompele
Jan Hellemans
 Center for Medical Genetics Ghent University Hospital, Belgium
 Application of normalisation and calculation software:
geNorm
qBASE
BioInformatics
P3
 Mikael Kubista
and collegues
MultiD Analyses AB
Lotsgatan, Göteborg, Sweden
www.multid.se
 GenEx for real-time PCR data analysis and sample classification.


Preliminary Matrix Workshop time table:


Wednesday afternoon  7th September 2005:
Opening of the Workshop and introductory talk by the WS organizers
 13:00 – 14:00
coffee break
 14:00 – 14:30
time frame 1:
 14:30 – 18:30


Thursday  8th September 2005:
time frame 2:
 08:30 – 11:30
Lunch in the cafeteria 
 11:30 – 12:30
time frame 3:
 12:30 – 15:30
variable coffee break
time frame 4:
 15:30 – 18:30


Friday  9th September 2005:
time frame 5:
 08:30 – 11:30
Lunch in the cafeteria
 11:30 – 12:30
time frame 6:
 12:30 – 15:30
Closing of the workshop
 15:30 – 16:00
The session instructors from the companies are asked to generate interesting sessions in which the student can learn about one distinct application or can solve one special problem. For session ideas and coordination of the sessionsm at the qPCR Matrix workshop arrangement please contact M. W. Pfaffl  qPCR2005@wzw.tum.de

Researchers and qPCR companies will be responsible for their own equipment: all hardware, software, kits and disposables. The organizers at the TUM-Weihenstephan can provide four different rooms (P1, P3 and P4) with different number of benches (from 7 to 17 benches, each fitting 4-6 students  and 1-2 session instructors), chairs, electricity, W-LAN, fridges, freezers, crushed ice, water, and…...
any changes =>  please contact the scientific organizer Michael W. Pfaffl via  qPCR2005@wzw.tum.de