Central
lecture halls complex in Freising – Weihenstephan, Technical University
Munich (TUM), 85354 Freising, Germany
Symposium & Workshops
agenda
Sessions
& Workshops overview
TATAA
Application Workshop ( fully booked )
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Sunday
4th September 2005
13:00 – 18:00 Built-up for Industrial Exhibition
15:00 – 18:00 Arrival & Registration
Monday 5th September 2005
Welcome & Opening of the Symposium
Lecture hall HS 14
08:00 – 10:00 Built-up for Industrial Exhibition
08:00 – 10:00 Arrival & Registration
09:00 – 10:00 Welcome Coffee & Tea
10:00 Welcome &
Opening of the Symposium.
Michael W. Pfaffl & Neven Zoric
Scientific coordination of the qPCR 2005 Symposium & TATAA Application
Workshop
10:10 Welcome at the Center of Food & Life Science
in Freising Weihenstephan.
Prof. Dr. Dr. h.c. mult. Wolfgang A. Herrmann, President TUM, Germany
10:20 Keynote lecture:
Real-time PCR, a personal perspective.
Russell Higuchi, "Pioneer in real-time PCR”
Associate Director of the Human Genetics Department, Roche Molecular
Systems, Almeda, CA, USA
Session: Pre-Analytical
Steps
Chair: V. Benes
Lecture hall HS 14
11:00 Session introduction by V. Benes
11:10 mRNA quantification from archival cancer samples.
Stephen A Bustin, Rebecca Hands, Sina Dorudi, Institute
of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry,
University of London
11:40 Nucleic acid isolation for diagnostic testing
using Bayer´s magnetic particles.
Guido Hennig, Bayer HealthCare AG, Diagnostics Research Germany, Leverkusen
12:10 Robust molecular profiling from RNA derived
of archival tissue.
Janine Antonov, Departement of Clinical Research, University of Bern,
Switzerland.
12:40 – 13:40 Lunch in the student cafeteria
13:40 Nucleic Acid Stabilization in Cultured Cell
and Tissue Lysates for QPCR Gene Expression Analysis.
L. Scott Basehore, Sr. Research Associate, Stratagene Research &
Development Department
14:10 Standardization of RNA Quality Assessment
using the RNA Integrity Number (RIN) and the 2100 bioanalyzer.
Marc Valer, Agilent Technologies, Waldbronn, Germany LIVE presentation
of Bioanalyzer 2100
15:00 – 15:30 Coffee break
15:30 Influence of RNA matrix effect on qRT-PCR
results – an overview.
Michael W. Pfaffl, Simone Fleige, Physiology, Center of Life Science,
Weihenstephan, Technical University of Munich, Germany
16:00 Use of standardized mixtures of internal standards
in RT-PCR to generate validated biomarkers and to develop standardized
transcript abundance reference databases.
James Willey1, Elizabeth Peters 2, Charles Knight1, Erin Crawford,
1, Bradley Austermiller1, Terry Osborn2; 1: Medical University of
Ohio, Toledo, Ohio, United States. 2: Gene Express, Inc., Toledo,
Ohio, United States.
16:30 Optimization of reverse transcription for
two-step QRT-PCR: A comparison of RT priming methods and the addition
of a new enhancer for efficient removal of double-stranded DNA contamination.
Ian Kavanagh1, Stephanie Noel1, Chatu Rajapakshe1, Gerwyn Jones1,
Nicky Quispe1, Simon Baker(1, 2), Meg Martel1, 1: ABgene, Epsom,
United Kingdom. 2: Birkbeck, University of London, United Kingdom.
17:00 – 18:30 Refreshments
in the Industrial Exhibition, Get-together with the Companies
18:30 – 20:00 Poster
- Session
20:00 – 24:00 Poster
– Party
welcome by Prof. Heinrich H.D. Meyer
Salonorchester Karl Edelmann is presenting a variety
of international music
Poster Party is sponsored by Roche Applied Science
& Eppendorf
Tuesday
6th September 2005
Session: New Application
– part 1: single cells
Chair: M. Kubista
Lecture hall HS 14
08:00 Session introduction by M. Kubista
08:10 Gene expression profiling in single cells.
Anders Ståhlberg (1), Martin Bengtsson (1,2), Patrik Rorsman(2,3)
and Mikael Kubista (1)
1: Department of Chemistry & Bioscience / Molecular Biotechnology,
Chalmers University of Technology and TATAA Biocenter, Sweden. 2:
Department of Experimental Medical Science, Lund University, Sweden.
3: The Oxford Centre for Diabetes, Endocrinology and Metabolism, The Churchill
Hospital, Oxford, England.
08:40 Quantitative single-cell RT-PCR and calcium
imaging in acute brain slices.
Robert Blum, Guylaine M. Durand, Nima Marandi, Simone D. Herberger,
Arthur Konnerth, Ludwig-Maximilians-Universität, Germany.
09:10 Amplification based assays in nanoliter volume
range.
Andreas Dahl1, Marc Sultan1, Regine Schwartz1, Matthias Lange1, Alexander
Jung2, Michael Steinwand2, Kenneth Livak2, Hans Lehrach1 and Lajos Nyarsik1,
(1) Max Planck Institute for Human Genetics, Deutschland (2) Applied Biosystems.
09:40 Forensic and single-molecule assays of mitochondrial
DNA using LATE-PCR.
Arthur Reis, Lawrence J. Wangh, Brandeis University,
Boston, MA, USA
10:10 – 10:40 Coffee break
Session: New Application
- part 2: new methods
Chair: R. Higuchi
Lecture hall HS 14
10:40 Rapid Development of RT-PCR Assays for RNAi
Experiments Using Pre-designed LNA-probe Libraries.
Michael Boutros, Boveri-Group Signaling and Functional
Genomics, DKFZ, Heidelberg, Germany.
11:10 A Multiplex Branched DNA Assay for Parallel
Quantitative Gene Expression Profiling.
Michael Flagella*, Son Bui*, Zhi Zheng, Cung Tuong Nguyen, Aiguo Zhang,
Larry Pastor, Yunqing Ma, Wen Yang, Kim Crawford, Gary K. McMaster, Frank
Witney and Yuling Luo, Genospectra Inc., United States.
11:40 TripleHYB: A novel detection format for real-time
PCR.
Anne-Katrin Rost 1, Natalia Malchowa 2, Awad A. Osman 1, Thomas Köhler
1, 1: AJ Roboscreen GmbH, Delitzscher Strasse 135, D-04129
Leipzig, Germany. 2: Department of Microbiology, Faculty of Biotechnology,
Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland.
12:00 Correlation of microarray and quantitative
real-time PCR results.
Elisa Wurmbach, Mount Sinai School of Medicine, United States.
12:30 – 13:30 Lunch in the student cafeteria
Session: New Application
- part 3: multiplexing
Chair: S. Bustin
Lecture hall HS 14
13:30 The best of both worlds - New dyes for qPCR
for use in combination with probes.
Neven Zoric, Coordinator of the TATAA Biocenter,
Göteborg, Sweden.
14:00 qPCR pitfalls - primer and probe design /
fluorophore quencher combinations.
Clémence Beslin, Eurogentec, Belgium.
14:30 Going MULTI – how to easily achieve high multiplexing
in real-time PCR.
Andreas Missel, Associate Director R&D, QIAGEN
GmbH, Hilden
15:00 Plexor™ Real-Time Quantitative PCR Systems:
Multiplexed assays made easy.
Kyle Hooper, Promega Corporation, Woods Hollow
Road, Madison, WI, USA
15:30 Two-color multiplex assay
for the identification of Orthopoxes viruses with Real-Time LUX PCR.
Mohamed Aitichou 1, Sandrine Javorschi-Miller 2,
Sofi Ibrahim 1, Mark Andersen 2, 1: Virology Division, United
States Army Medical Research Institute of Infectious Diseases, United
States. 2: Invitrogen, United States.
16:00 – 16:30 Coffee break
Session: New Application
- part 4: mixed session
Chair: T. Bar
Lecture hall HS 14
16:30 ChIP studies on a HOX gene regulated by Polycomb
group and trithorax group proteins.
Bernadett Papp, EMBL, Germany.
17:00 The Ups and Downs of Gene Regulation: Validating
siRNA Gene Expression Disruption with RT-qPCR.
Hilary Katherine Srere, Bio-Rad Laboratories, United
States.
17:30 Real-time immuno-PCR for quantification of
proteins.
Kristina Lind, Mikael Kubista; Department of Chemistry and Bioscience,
Chalmers University,
18:00 microRNA expression profiles from Real-time
PCR classify ES and differentiated cells
Simone Guenther 1, Adam Broomer 2, Dana Ridzon 2, Kai Lao2, Karl Guegler2,
William Strauss3; 1: Applied Biosystems, Darmstadt, Germany.
2: Applied Biosystems, Foster City,USA. 3: University of Colorado,
Boulder, USA.
19:00 – 24:00 Symposium Gala Dinner
Location: Lindenkeller, Pasta & More, Freising
· Bavarian Buffet
· Mediterranean Buffet
· Asian Buffet
· Modern Crossover Buffet
· Music and Dancing
Tuesday
6th September 2005
Session: Normalization
Chair: N. Zoric
Lecture hall HS 15
08:00 Session introduction by N. Zoric
08:10 Normalization of gene expression: state of
the art and preview on a new strategy using expressed Alu repeats.
Jo Vandesompele, Center for Medical Genetics Ghent,
Ghent University Hospital, Ghent, Belgium
08:40 Normalisation of mRNA levels against total
DNA content.
Shu-Rui Li 1, Doug Storts 2, Becky Hands 1, Benjamin
Krenke 2, Ethan Strauss 2, William Ogunkolade 1, Stephen Andrew Bustin
1, 1: Queen Mary University of London, United Kingdom. 2: Promega
Corporation, USA, Institute of Cell and Molecular Science, Queen Mary's
School of Medicine and Dentistry, University of London
09:10 Normalization genes for heart failure myocardium
in mice, rats and humans.
Trond Brattelid (2,3), Lisbeth Winer (1), Ole M. Sejersted (1,3) and
Kristin B. Andersson (1,3)
1: Institute for Experimental Medical Research, Ullevaal University
Hospital, University of Oslo. 2: Department of Pharmacology,
University of Oslo. 3: Center for Heart Failure Research,
Faculty of Medicine, University of Oslo.
09:40 Early mouse development and mammalian embryonic
stem cells: a qRT-PCR story.
Erik Willems1, Caroline Kemp1, Ileana Mateizel2, Karen Sermon2 and
Luc Leyns1, 1. Lab for Cell Genetics, Vrije Universiteit Brussel,
Brussels, Belgium. 2: Research Centre for Reproduction and
Genetics, Vrije Universiteit Brussel, Brussels, Belgium.
10:10 – 10:40 Coffee break
Session: Optimisation
– part 1
Chair: B. Rutledge
Lecture hall HS 15
10:40 Session introduction by b. Rutledge
10:50 Design and optimization of Taqman and SYBR
Green I real-time qPCR assays.
Greg Shipley, Director, Quantitative Genomics Core, Laboratory, The
University of Texas Health Science Centre- Houston, USA
11:20 Comparison of MMP gene expression analysis
by capillary and "realplex" real-time PCR.
Raimund Kinne, Experimentelle Rheumatologie, Klinikum
der Friedrich-Schiller-Universität Jena, Germany
11:50 Finding the needle in the haystack - LNA bases
enhance SNP detection dramatically.
Olfert Landt, TIB MOLBIOL Syntheselabor GmbH Eresburgstraße,
Berlin, Germany
12:20 Infectious disease diagnostic research in
Africa; the role of real time PCR.
Jim Huggett, Centre for Infectious Diseases &
International Health, University College London, UK
12:50 – 13:50 Lunch in the student cafeteria
Session: Optimization
– part 2
Chair: H.H.D. Meyer
Lecture hall HS 15
13:50 Relative real time PCR for gene expression
measurement in breast cancer biopsies.
A.Larionov1, S.White1, D.B.Evans2, A.Krause2, M.J.Dixon1, W.R.Miller1;
1: Breast Research Group, Western General Hospital, Edinburgh, UK. 2: Novartis
Pharma AG, Basel, Switzerland.
14:20 The fitness of a football team: High Resolution
Melts for the determination of genotypes.
Valin Reja1, Brant Bassam1 and Thomas Kaiser2;
1: Corbett Research, Mortlake, NSW, Australia. 2: Corbett Research
UK Limited, Cambridge Science Park, Milton, Cambridge, UK.
14:50 Validation of fast PCR protocols with the
Eppendorf Mastercycler ep realplex.
Cynthia Potter, Eppendorf UK Limited, Vision Park, Chivers Way, Histon,
Cambridge, UK
15:20 – 15:50 Coffee break
Session:
Standardization
Chair: G. Shipley
Lecture hall HS 15
15:50 Session introduction by G. Shipley
16:00 A Comparison of Real-Time RT-PCR Technique,
Chemistries and Instrumentation in Laboratories Utilizing the Same Assay.
Pamela Scott Adams, Director, Molecular Biology
Core Facility, Trudeau Institute, Saranac Lake, NY, USA
16:30 Accurate Gene Expression Analysis with High
Flexibility: Concepts and Developments.
Oliver Geulen, Roche Applied Science, Mannheim, Germany
17:00 The Data Comparability Challenge - Standards
and Best Practices.
Morten T. Andersen, Bio-Molecular Innovation, LGC, Teddington, Middlesex,
UK
17:30 Putting the "quantity " into quantitative
PCR: A simplified approach to the establishment and application of quantitative
scale.
Bob Rutledge, Natural Resources Canada, Canadian Forest Service, Laurentian
Forestry Centre, Sainte-Foy, Quebec, Canada
18:00 Assay standardisation using universal internal
controls and lyophilized reagent beads.
Andreas Eckelt, Cepheid SA, Deutschland.
19:00 – 24:00 Symposium
Gala Dinner
Location: Lindenkeller, Pasta & More, Freising
· Bavarian Buffet
· Mediterranean Buffet
· Asian Buffet
· Modern Crossover Buffet
· Music and Dancing
Wednesday
7th September 2005
Session: BioInformatics
Chair: M. W. Pfaffl
Lecture hall HS 14
08:00 Session introduction by M. W. Pfaffl
08:10 From Sequences to Synthesis: Optimal Amplification
through Careful Oligonucleotide Selection.
Ben Sowers, Research Associate, Biosearch Technologies
08:40 Estimation of sample specific efficiency –
methods and applications.
Tzachi Bar, Department of Chemistry and Biosciences Chalmers University
of Technology, Göteborg, Sweden;
Ales Tichopad, LabonNet, Kirchheim near Munich, Germany
09:10 Amplification efficiency dynamics and its
implications: Developing a kinetic-based approach for quantitative analysis.
Bob Rutledge, Natural Resources Canada, Canadian
Forest Service, Laurentian Forestry Centre, Sainte-Foy, Quebec, Canada
09:40 qBase: relative quantification software for
management and automated analysis.
Jan Hellemans, Center for Medical Genetics Ghent, Ghent University
Hospital, Ghent, Belgium
10:10 – 10:40 Coffee break
10:40 Early Phase Fluorescence Fitting of real-ime
PCR reaction.
Hervé Rhinn, Laboratoire de Pharmacologiqe Chimique et Génétique,
France.
11:10 Classification of real-time PCR data.
Mikael Kubista, Professor of Biotechnology,
MultiD Analyses AB, Göteborg, Sweden
11:40 The real-time PCR primer and probe database
RTPrimerDB: a major update.
Filip Pattyn, Piet Robbrecht, Jelle Verspurten, Anne De Paepe, Frank
Speleman, Jo Vandesompele, Center for Medical Genetics Ghent (CMGG),
Ghent University Hospital, Ghent, Belgium.
12:10 Closing of the
Symposium in HS 14
Michael W.
Pfaffl
Wednesday
7th September 2005
Session: GMO Analytics
& Food Hygiene
Chair: C. Albrecht
Lecture hall HS 15
08:00 Session introduction by C. Albrecht
08:10 Keynote lecture:
Uncertainties and certainties in GMO analytics using qPCR.
Philipp Hübner, Kantonales Laboratorium Basel-Stadt, Abteilungsleiter
Lebensmittel, Basel, Switzerland.
08:50 Accurate GMO quantification in food samples.
Dörte Wulff, Research and Development, Eurofins Genescan / GeneScan
Analytics GmbH, Freiburg, Germany
09:20 The USDA/GIPSA Proficiency Program: A Summary
of Participants Capabilities for Detecting and Quantifying Transgenic
Events in Corn and Soybeans.
Ron Jenkins, USDA/GIPSA, USA
09:50 - 10:30 Coffee break
10:30 Application of synthetic DNA-standards for
the quantitative screening of different genetically modified rapeseed lines
via real-time PCR.
Francisco Moreano, Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit
(LGL), Infektologie / Molekularbiologie, German
11:00 Cloned plasmid DNA molecules as a tool for
GMO analysis.
Isabel Taverniers, Marc De Loose
Department of Plant Genetics and Breeding, DvP-CLO, Melle, Belgium.
11:30 Detection of Food Pathogens using the Smart
Cycler II.
Martina Fricker, Dep. of Bioscience, Technical University of Munich,
Freising, Germany
Closing of the Symposium
Lecture hall HS 14
12:10 – 12:20 Closing of the Symposium.
Michael W. Pfaffl
12:20 – 13:00 Lunch in the student cafeteria
Symposium & Workshops
agenda
Sessions & Workshops
overview
|