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Comment about the Gene Quantification page in Science - LIFE SCIENCE TECHNOLOGIES

LIFE SCIENCE TECHNOLOGIES - qPCR Innovations and Blueprints PDF
7. October 2011  by Chris Tachibana  in Science

Quantitative PCR users can rapidly generate large amounts of high-quality data with new instruments and products made possible by microfluidics and miniaturization technology. These platforms are the tools for developing techniques that require extremely high throughput and sensitivity such as digital PCR and single-cell analysis. Researchers are adopting these methods to ask sophisticated questions about genetics and cancer biology as well as to develop novel research and diagnostic assays. As qPCR innovators explore new frontiers and everyday users venture into more complicated workflows, international groups of industry and academic partners are keeping us on the path of best practices. Two consortia (MIQE & SPIDIA) are generating guidelines on the qPCR process - from experimental design and pre-analysis sample collection, to processing data and publishing results. The guidelines are blueprints that ensure reproducibility, validity, and transparency.

Citations from Science -
http://www.sciencemag.org

"The Gene Quantification website is like a 7-Eleven store for quantitative polymerase chain reaction (qPCR): a one-stop shop for news, advice, and product links. Its curator is Michael W. Pfaffl, a professor in the physiology department at Technical University of Munich, who has been developing qPCR methodology for more than a decade. Pfaffl sees qPCR innovation in three areas: platforms, technologies, and physiology. ... ..."

"... ... With the economies of scale and quality assurance that core facilities or contract research organizations can provide, outsourcing qPCR work is tempting. Researchers still need to understand good data handling and experimental design, though, says Kubista. "The user doesn't need to be a statistician, but has to understand the purpose of different controls and replicates." It's a lot to keep track of, with technological advances leading researchers in many directions; but a regular stop at the Gene Quantification website will keep you stocked up on current qPCR news and developments."






2004
total page views
total unique sites
per month
unique user sessions
per 24 hours

data transfer
in  [ GB ]
June 2004
63 486
8 634
9 895
  5.421
July 2004
78 719
9 822
11 598
  9.483
August 2004
54 371
9 672
10 612
13.199
September 2004
58 526
9 274
10 336
13.881
October 2004
68 490
10 542
12 980
16.662
November 2004
66 127
11 257
11 725
18.766
December 2004
50 904
8 880
10 420
15.203
total 2004
440 623
68 081
77 566
92.535
average/month in 2004
62 950
9 725
11 080
13.219
2005




January 2005
60 113
10 960
12 405
17.512
February 2005
53 252
8 566
11 643
18.241
March 2005
61 099
9 982
14 837
23.246
April 2005
57 415
11 945
18 176
24.274
May 2005
54 143
11 402
17 673
21.011
June 2005
57 720
11 928
18 436
23.624
July 2005
50 337
11 833
19 461
23.019
August 2005
50 127
11 601
18 171
21.867
September 2005
49 478
10 754
15 990
21.423
October 2005
55 420
11 616
16 956
23.063
November 2005
62 648
13 017
19 375
28.479
December 2005
50 599
11 129
16 630
25.245
total 2005
698 161
134 553
199 753
271.004
average/month in 2005
58 180
11 215
16 650
22.585
2006




January 2006
58 660
13 813
20 832
24.309
February 2006
62 465
15 271
23 365
30.715
March 2006
76 467
18 391
29 610
42.822
April 2006
63 779
17 640
27 047
32.362
May 2006
66 903
33 700
61 156
30.522
June 2006
67 410
43 399
69 638
31.912
July 2006
69 982
36 821
55 766
30.920
August 2006
57 133
28 239
41 274
26.588
September 2006
65 776
30 217
37 957
28.856
October 2006
75 322
28 770
36 941
29.591
November 2006
71 258
26 328
36 221
27.822
December 2006
67 248
27 745
34 775
27.880
total 2006
802 450
320 335
474 580
364.510
average/month in 2006
66 900
26 700
39 550
30.376
2007




January 2007
70 531
24 985
44 743
35.030
February 2007 65 562
21 679
38 995
31.684
March 2007 69 456
27 729
35 356
30.139
April 2007
77 557
26 774
34 567
33.755
May 2007
84 256
28 971
39 338
35.987
June 2007
72 204
23 596
31 983
44.150
July 2007
78 506
23 708
37 685
37.255
August 2007
74 747
22 584
32 313
33.261
September 2007
84 270
34 089
52 656
41.650
October 2007
89 106
35 039
50 376
70.986
November 2007
88 760
34 935
50 045
66.528
December 2007
82 840
31 135
46 405
52.752
total 2007
937 860
337 2540
494 575
513.180
average/month 2007
78 155 28 105 42 215
42.765
2008




January 2008
86 970
34 460
44 305
48.654
February 2008
85 320
40 130
45 290 60.692
March 2008 82 356
37 672
42 339
57.381
April 2008
89 911
39 491
46 732
52.868
May 2008
88 590
39 044
44 565
48.317
June 2008
91 291
40 245
41 355
50.698
July 2008
87 610
41 562
42 252
51.532
August 2008
81 355
33 392
39 392
41.055
September 2008
86 027
40 051
43 051
47.153
October 2008
94 177
45 705
49 418
59.610
November 2008
104 120
52 460
57 952
62.320
December 2008
112 855
72 886
73 372
54.850
total 2008
 1 080 605
520 100
570 050
635.170
average/month 2008
90 050
43 350
47 500
52.930
2009



January 2009
83 760
53 750
54 250
61.564
February 2009
77 620
49 458
50 060
85.534
March 2009
101 920
62 480
67 070
69.756
April 2009
87 867
53 655
58 684
70.971
May 2009
82 085
46 988
52 438
71.865
June 2009 88 008
44 438
49 832
75.805
July 2009 85 471
52 695
54 347
77.335
August 2009
78 229
46 854
49 863
58.326
September 2009
89 783
48 190
50 386
63.235
October 2009
105 774
60 274
65 641
78.923
November 2009
103 628
58 928
64 931
83.024
December 2009
93 713
56 921
64 519
78.115
total 2009
1 077 900
634 650
682 070
875.500
average/month 2009
89 820
52 890
56 850 72.880
2010



January 2010
90 260
67 180
74 430
84.885
February 2010
88 480
65 950
72 460
113.230
March 2010
106 034
72 103
77 986
112.928
April 2010
103 105
75 967
80 238
105.063
May 2010 105 716
76 558
84 096
106.198
June 2010
107 592
72 948
82 464
95.949
July 2010
99 987
71 061
81 920
93.907
August 2010
97 293
66 321
75 960
86.441
September 2010
103 812
75 299
82 805
92.494
October 201
116 414
79 392
87 341
98.652
November 201
126 442
79 168
86 761
96.272
December 2010
101 542
79 548
87 504
87.983
total 2010
1 247 695
881 680
973 975
1,174.000
average/month 2010 103 975
73 475
81 165
97.835
2011




January 2011
115 371 91 994
100 995
101.456
February 2011
107 206
83 261
91 410
90.937
March 2011
120 137
95 524
107 951
105.416
April 2011
108 365
83 378
92 715
93.353
May 2011
112 326
80 804
88 541
95.741
June 2011
111 386
77 745
91 604
95.011
July 2011
116 333
78 425
88 447
93.617
August 2011
116 202
76 675
85 997
95.255
September 2011
121 911
85 527
93 141
114.200
October 2011
140 355
94 104
103 577
108.132
November 2011
147 357
98 050
108 710
121.984
December 2011
139 859
95 430
106 175
115.259
total 2011
1 456 800
1 040 930
 1 186 275
1,230.360
average/month 2011 121 400
86 750
98 850
102.530
2012




January 2012
139 278
92 126
102 323
114.256
February 2012
145 342
95 991
105 543
112.961
March 2012
167 531
101 705
112 857
130.316
April 2012
145 579
91 358
104 306
124.617
May 2012
146 889
92 060
104 843
134.133
June 2012
133 946
95 096
101 141
128.437
July 2012
125 944
87 975
98 883
116.595
August 2012
122 118
86 876
96 777
114.381
September 2012
125 368
88 441
104 565
117.588
October 2012
133 956
93 018
107 338
126.033
November 2012
140 107
95 993
106 123
135.726
December 2012
157 826
101 757
112 114
146.903
total 2012 1 673 900
1 122 400
 1 356 850
1,492.145
average/month 2012 139 500
93 550
113 070
124.350
2013




January 2013
159 814
100 388
127 004
135.491
February 2013
168 411
98 877
124 438
131.374
March 2013 165 482
105 722
134 523
136.778
April 2013
168 767
101 941
133 056
135.571
May 2013
162 472
121 440
138 969
122.701
June 2013
173 118
126 075
142 756
128.044
July 2013
164 332
122 083
137 093
125.030
August 2013
177 809
132 892
135 946
128.872
September 2013
184 734
134 355
147 053
142.903
October 2013
186 880
136 565
154 898
151.352
November 2013
178 930
119 857
143 009
138.802
December 2013
170 457
121 335
144 716
140.281
total 2013
2 061 212
1 421 537
1 663 465
1,617.199
average/month 2013 171 760
118 460
138 620
134.770
2014




January 2014
175 355
121 824
148 650
142.553
February 2014
173 451
120 993
140 994
136.808
March 2014
169 880
117 529
136 583
133.634
April 2014
167 093
116 085
137 782
140.057
May 2014
174 382
121 654
148 063
148.054
June 2014
173 072
122 805
146 050
145.427
July 2014
175 338
125 823
149 876
153.787
August 2014
183 329
131 045
152 330
147.431
September 2014
194 690
129 388
148 893
149.506
October 2014
195 393
127 482
151 360
147.035
November 2014
193 914
131 672
144 245
151.428
December 2014
193 529
130 805
141 893
152.527
total 2014
2 161 420
1 497 110
1 756 730
1,750.250
average/month 2014
180 120
124 760
146 390
145.85
2015




January 2015
186 322
132 217
153 353
163.443
February 2015
195 385
147 102
148 363
168.415
March 2015
172 334
144 689
150 395
173.330
April 2015
180 532
148 138
153 231
180.425
May 2015
192 871
170 922
180 753
216.044
June 2015
185 664
163 805
173 080
198.727
July 2015
191 366
168 921
176 434
204.853
August 2015
183 403
171 064
181 334
196.980
September 2015
196 346 179 879
185 983
212.045
October 2015
192 610
168 902
183 573
190.336
November 2015
197 374
181 055
184 425
211.841
December 2015
190 983
169 225
176 005
195.077
total 2015
2 277 980
1 945 950
2 046 930
2,311.486
average/month 2015
189 840 162 160
170 570
192.620
2016




January 2016
188 367
170 509
175 607
200.641
February 2016
175 342
165 208
167 005
186.683
March 2016 191 690
185 643
177 652
195.974
April 2016
193 452
173 830
184 589
205.583
May 2016
194 997
173 106
185 215
203.584
June 2016
192 760
179 675
183 894
196.073
total 2016 1 136 600
1 047 970
1 073 960
1,188.538
average/month 2016 189 450
174 660
178 995
198.090

total page views
per month
total unique sites
per month
unique user sessions
per 24 hours
data transfer
in  GB per month

Home page views and server statistics

March 2002 - May 2004

http://www.wzw.tum.de/gene-quantification/

date
since 26.02.2002

2002

total  visits
in
gene-quantification
visits  on
homepages
data
transfer
in  
[ GB ]
REST©  &
REST-XL©&
BestKeeper©
visits &
 downloads
paper & poster
downloads
Pfaffl et al. workgroup
March 2002
7 930
1 549
0.209
253
576
April 2002
19 406
3 465
0.722
567
2 029
May 2002
 23 802
3 233
1.139
414
 3 972
June 2002
 20 311
 2 585
 0.988
383
 4 190
July 2002
 32 201
 3 337
 1.540
472
 4 502
August 2002
 36 759
 4 372
 1.845
419
 3 967
September 2002
 55 168
 6 971
 2.730
 412
 5 237
October 2002
 64 483
 6 717
 3.726
 509
 5 741
November 2002
 52 507
 4 954
 3.099
 447
 5 180
December 2002
 51 416
 4 567
 3.368
 397
 5 360
 total  2002
 363 982
 41 750
 19.366
4 273
40 754
2003          
 January 2003
 67 621
 5 985
 4.501
 562
 7 773
February 2003
 75 859
 6 657
 5.321
 635
 9 255
March 2003
 80 011
 7 117
 5.280
 929
 9 297
April 2003
 81 292
 6 848
 5.794
 933
 9 989
May 2003
 80 287
 6 660
 5.429
 867
 9 857
June 2003
 76 561
 6 853
 5.425
 562
 9 484
July 2003
 75 413
 5 823
 5.169
 746
 11 489
August 2003
 62 922
 4 702
 4.730
 663
 11 864
September 2003
 78 350
 4 864
 5.636
 757
 11 676
October 2003
81 209
 7 356
 7.792
 589
 14 248
November 2003
 87 697
 6 115
 6.662
 547
 12 406
December 2003
 78 273
 7 247
 5.867
 709
 11 679
total  2003
953 495
 76 226
67.606
 8 499
129 017
 2002 + 2003
1 307 477
117 976 
86.972
12 772 169 771
 January 2004
67 843
7 611 6.342
  894 13 654
February 2004
72 449
7 923 7.987
792
12 753
March 2004
67 331
9 118
8.391
829
14 769
April 2004
59 948
8 518
9.042
755
13 839
May 2004
63 445
8 895
7.567
692
15 991
total 
03/2002 - 05/2004
  1 577 890
160 141
  126.285
  16 735  240 800


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