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Agilent Technologies is a worldwide leader in developing innovative products and technologies for life science research. Our Stratagene products offer a total solutions approach to quantitative PCR, simplifying the challenges you face in sample preparation as well as data analysis and validation. Whether or not you are experienced in QPCR your individual needs are met with our comprehensive range of products and supports. Researchers just getting started in QPCR will benefit from our on-site and web-based training programs, premixed kits, and turnkey instruments installation. More experienced users appreciate the flexibility of our powerful, user friendly software as well as reagent kits.

 
Mx Family of QPCR Systems

The Mx3005P QPCR System – superior flexibility and performance
  • Advanced five channel optical system with user-selected filters for greater flexibility
  • Defined excitation and emission detection wavelengths are ideal for superior multiplex results of up to five targets simultaneously
  • Independent Filter Control for greater flexibility
  • MxPro QPCR Software - the most powerful data analysis software available now with optional 21 CFR Part 11 compatible features
  • The best QPCR System Service Program available
  • Open flexible platform design supports all fluorescent chemistries and a wide range of applications
  • Recommended for use with Stratagene's Brilliant® QPCR reagent kits
The Mx3000P QPCR System – the first full-featured, high-performance QPCR system at a price you can afford
  • Four optical channels with user-selected filters for greater flexibility
  • Defined excitation and emission detection wavelengths are ideal for superior signal discrimination
  • MxPro QPCR Software - the most powerful data analysis software available now with optional 21 CFR Part 11 compatible features
  • The best QPCR System Service Program available
  • Recommended for use with Stratagene's Brilliant® QPCR reagent kits


  QPCR & QRT PCR Reagents

Brilliant II Probe-Based QPCR and QRT-PCR Master Mix
  • Earlier Ct detection and improved sensitivity of detection
  • Improved data reproducibility
  • Greater flexibility for use with numerous different templates and targets
  • More accurate quantification results
  • Uses AffinityScript Mutliple Temperature Reverse Transcriptase
  • Developed on our Mx QPCR Systems
Brilliant Multiplex QPCR Master Mix
  • Amplifies up to 4 targets in a single reaction
  • Simultaneously amplifies low and high abundance targets
  • Maximizes analysis of limited or rare samples
  • Allows detection of multiple targets plus an internal control
  • Saves time and increases throughput, reduces cost per reaction
Brilliant Probe-Based QPCR and QRT-PCR Reagents
  • Master mixes and core reagent kits for any fluorescent chemistry
  • Compatible with sequence-specific probes (probe not included)
  • Wide linear dynamic range of amplification
  • SureStart Taq DNA polymerase provides high-specificity hot-start
  • Passive reference dye included in separate tube for flexiblity
  • Core kits available for assay optimizations
Brilliant SYBR® Green QPCR and QRT-PCR Reagents  
  • Universal detection with SYBR® Green dye (included)
  • Superior sensitivity and specificity compared to competitors' SYBR® Green kits
  • Choice of master mix and core reagent kit formats
  • SureStart Taq DNA polymerase provides high-specificity hot-start
  • Passive reference dye included in a separate tube to maximize performance on different instrument platforms
Brilliant II QPCR & QRT-PCR Reagents with High and Low ROX
  • Developed for QPCR systems requiring ROX reference dye
  • Delivers superior results on Stratagene and ABI real-time QPCR systems
  • Improved sensitivity of detection and more robust amplification
  • Improved performance with earlier Ct detection across a wide dynamic range
  • Increased sensitivity of detection to low copy numbers
  • Reliable quantification across a wide range of targets and templates
SideStep™ and SideStep™ II QPCR and QRT-PCR Master Mixes
  • Streamlined sample preparation for QPCR in 10 minutes
  • RNA stabilization at least 20 months at -80C prevents loss or degradation
  • Gene expression down to a single cell
  • Genomic DNA removal reagents allows use of current primer sets
  • Prepare lysates from as few as one cell or up to 1 million cells
  • No toxic organic chemicals or ethanol precipitations
  • Both one-step (probe-based) and two-step (SYBR® Green) formats available
Alien QRT-PCR Inhibitor Alert
  • High-quality external control for detecting inhibitors in RNA samples for QRT-PCR experiments
  • High sensitivity to various inhibitors
  • No significant homology to any known sequence
  • Ideally suited for assay standardization applications
Stratagene QPCR Reference Total RNA, Human and Mouse
  • High-quality total RNA control for quantitative PCR gene expression analysis
  • An optimized blend of RNA for either human or mouse, derived from cell lines representing different tissue
  • Greater consistency than RNA references derived from tissue sources
  • Maximum representation of low, medium and high abundant gene transcripts
  • Manufactured at industrial scale lot sizes and stringently quality-controlled for minimized lot-to-lot variation
  • No detectable levels of genomic DNA


Sample Preparation

Absolutely RNA FFPE Kit
  • Reliable recovery of RNA suitable for QRT-PCR from formalin fixed paraffin embedded tissues
  • High yields of unmodified and QRT-PCR amplifiable RNA
  • Highly Pure DNA-free total RNA
  • Kit offers you the ability to cross compare samples and experiments
  • Complete removal of genomic DNA contamination
  • Easy-to-use protocol
  • No hazardous waste removal or storage issues
Absolutely RNA Purification Kits
  • Fast and Simple purification of DNA-free total RNA from tissue and cell samples
  • Isolates highly pure RNA
  • Flexible for all sample sizes
  • Minimizes loss of RNA
  • Eliminates DNA contamination
  • Saves times with streamlined method.
  • Superior multiplexing performance
SideStep Lysis and Stabilization Buffer
  • Equivalent results compared to purified RNA
  • Minimize RNA loss and ensure true, actual, accurate gene expression data
  • Samples are ready for quantitative reverse-transcriptase PCR (QRT-PCR) in 10 min
  • RNA is stable up to 6 months at -20C
  • Easy to use protocol produces lysates using up to 1 x 10^6 cells
  • Widest range of cell input per reaction that can be scaled up or down
SideStep QPCR cDNA Synthesis Kit
  • Produce high quality, QPCR-grade cDNA directly from cells
  • Immediate cell lysis and RNA stabilization up to 6 months at -20C
  • Easy to use protocol produces lysates using up to 1 x 10^6 cells
  • cDNA expressly for QPCR amplification using SYBR® Green or probe-based detection
AffinityScript QPCR cDNA synthesis kit
  • Fast, highly efficient cDNA synthesis for QRT-PCR
  • Multiple Temperature RT includes for maximum flexibility
  • Streamlined protocol produces cDNA in 15 minutes
  • Master mix format saves time, reduces pipetting variability
  • Linear detection from 3 pg to 3 µg total RNA
AffinityScript Multiple Temperature Reverse Transcriptase
  • Robust cDNA synthesis across a broad range of temperatures from 37°C – 55°C
  • Full length cDNA synthesis from 500 bp up to 20 kb
  • High affinity for RNA:DNA primer:template complexes promotes robust cDNA yield
  • Ability to reverse transcribe through secondary structure
  • Detects low input amounts of RNA better than SuperScript® III and SuperScript® II RT
  • Strict enzyme purity specifications ensures no contaminating RNase and exonuclease activities



Services Mx3000P and Mx3005P System Service Program
                                                                           
Our Service Program redefines the service and repair standard for QPCR instrumentation by guaranteeing the shortest instrument down time in the industry

  • Factory Service Center repair with guaranteed loaner instrument for duration of repair within 2 business days of a service call
  • Pre-paid shipping, free software and hardware upgrades
  • Qualified repair parts, post-repair factory level quality control and full system validation
  • One year warranty included with purchase of Mx3000P and Mx3005P Systems
  • Reagent usage service credit
Fast Track Education Program

The Fast Track QPCR Education Program is a comprehensive program of user training and advanced education for users of our real-time PCR instruments. The program provides a wide range of tools to get you up and running with real-time PCR quickly and effectively. The program also offers you access to expert Field Application Scientists providing advanced education. As a leader in the training of scientists on real-time PCR, we are proud to offer the Fast Track Education Program.
  • Rapid and effective QPCR training
  • Advanced application-focused education
  • On-demand educational tools
  • Ongoing education to support new personal and evolving assays
  • Consultation with expert Field Application Scientists
More information available on  http://www.stratagene.com/tradeshows/fasttrack.aspx

Stratagene also offers a variety of live and recorded online seminars presented in real-time by one of our Technical Services Scientists and Tutorials.
For more information and registration, please visit our website



Contact

Contact a rep

inquiries@agilent.com

www.stratagene.com/contacts/


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