Illumina
introduces a new Real-Time PCR Resource Center, filled with all the
information you need to support your research.
And we mean everything, from Real-Time PCR 101 audio training modules
to data analysis guides for the experienced user, FAQs to help you
quickly resolve issues, and links to webinars where you can learn even
more.
Scientists worldwide trust real-time PCR reagents from Takara Bio–even
for the most demanding qPCR experiments.
When
you use real-time PCR reagents that are sensitive and specific, you can
spend less time on PCR troubleshooting and more time on moving your
research forward. Takara Bio offers qPCR kits for use with both SYBR
Green detection and TaqMan probes.
Part
1: Getting You Started
Find
the right detection method
Study
your gene expression by RT-qPCR
Check
the accuracy of your results
Speaker: Professor Mikael
Kubista, TATAA Biocenter
Compatible
with all major real time
PCR instrument systems, these products allow you to obtain accurate,
consistent results from a wide variety of sample types, even when other
reagents fail. Want to try Takara Bio real time PCR reagents for
yourself? Samples are available for many of our qPCR reagents.
Part 2: Interpreting Your Data
Determine
Cq values
Obtain
absolute quantification of your starting DNA
Define
a good reference for relative quantification
Follow
MIQE guidelines
Speaker: Robert
Sjöback, Ph.D., TATAA Biocenter
Count
on Takara Bio to help you choose the best qPCR products for plant,
soil, blood, paraffin-embedded tissues, archival or degraded samples or
when conducting gene expression studies, forensic research analyses, or
other specific applications. Want to learn more about real-time PCR?
Three videos produced by TATAA Biocenter in Europe help you understand
everything from the basics of qPCR to data analysis techniques. Each
20- to 30-minute video can be watched at your convenience from the
comfort of your office, lab, or home.
Part 3: Troubleshooting Your Results
Identify
potential pitfalls in qPCR data
Detect
PCR inhibition
Control
the quality of RNA and reverse transcription
Whether you’re new to PCR or need more in-depth learning guides our
video library will have all the resources you need. See our
Veriti® Thermal Cycler in action, catch up with Ph.Diddy and
Ph.Diva and go behind the scenes. New videos will be added periodically
so be sure to bookmark this page for future reference.
Featured Videos
Music Videos
Applications
Products
How To's
Interviews
The
MIQE guidelines - Online tutorials
The MIQE Guidelines Uncloaked - Speaker:
Greg Shipley 8 June 2010 -The
MIQE (Minimum Information for Publication of
Quantitative Real-Time
PCR Experiments) guidelines have been presented to serve as a practical
guide for authors when publishing experimental data based on real-time
qPCR. Each item is presented in tabular form as a checklist within the
MIQE manuscript. However, this format has left little room for
explanation of precisely what is expected from the items listed and no
information on how one might go about assimilating the information
requested. This presentation presents an expanded explanation of the
guideline items with commentary on how those requirements might be met
prior to publication.
As part of our customer education program, we have
provided two
recorded seminar series covering the topics of qPCR and MIQE. The
recorded sessions are intended to provide a high level overview of
these subject matters. We have kept the lessons concise so that you can
enjoy a self-paced learning program.
This animation describes Exiqon's
LNA™ technology, and why it is
superior to DNA in the study of microRNAs, which are challenging for
many reasons => show
animation
Their short length and the high sequence similarity
between closely
related microRNAs makes it hard to detect them with sufficient
specificity and sensitivity. => Exiquon
ProbeLibrary real-time PCR Assay System
Synopsis:
Thousands of users today trust the bioanalyzer 2100 for
qualifying total RNA samples, looking for integrity, purity, recovery,
and consistency information for sample preparation methods, assisting
them to determine the optimal conditions for their experimental design.
Marc Valer describes the use of Agilent's new RNA Integrity Database
(RINdb) to assist in the development of experimental protocols,
particularly sample preparations, by providing a means for researchers
to contextualize RNA profiles.
A Statistical
approach to qPCR gene expression data analysis
Register for a live Webinar and hear a live talk about the cutting-edge
technologies of your choice followed by a Q&A session. The speaker
will answer as many of your questions as possible during the session.
Any remaining questions will be answered by personal e-mail after the
Webinar. Alternatively, you can listen to one of our previously
recorded Webinars.
miRNA purification
and detection — new tools in expression profiling and biomarker
development
Recent progress in
RNAi screening
A successful and
affordable RNAi solution for every lab
Novartis scientists
share their experiences in multiplex, real-time PCR
Transcriptome-wide
miRNA quantification by RT-PCR
Accelerate your PCR
and qPCR
and much
more..........................
PODCAST:
The Introduction
to Molecular and Cellular Biology lectures include
text, images, and audio. Each lecture webpage is synchronized to the
audio
component. In addition, the lectures are available as a podcast
subscription.
by Lawrence
Chasin and Deborah Mowshowitz, Department of
Biological Sciences, Columbia University, New York. Clickable pictures are from Purves, et. al., Life,
5th Edition,
Sinauer-Freeman's Images of Life 5.0. A
production of the Columbia
Center for New Media Teaching and Learnin
The
presentations listed below provide background and instruction about
specific concepts in PCR.
PCR Fundamentals— This audio slideshow
describes the PCR process, including the steps and reaction components
involved. for Windows (.wmv) | for Macintosh (.mov)
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
Understanding
biological complexity arising from patterns of gene expression requires
accurate and precise measurement of RNA levels across large numbers of
genes simultaneously. The preferred method for
quantitative transcriptional analysis is real time PCR (rt-PCR) in a
96- or 384-well microtiter plate. Scaling rt-PCR to higher throughputs
is intrinsically limited by cost and logistic considerations. Alternatively
hybridization microarrays are capable of measuring transcription of
many thousands of genes simultaneously yet are limited by low
sensitivity, accuracy and sample throughput. We
demonstrate a hybrid approach by combining the superior accuracy,
precision and dynamic range of rt-PCR with the high density parallelism
of a microarray in an array of 3,072 real-time, 33 nL polymerase chain
reactions the size of a microscope slide. Real-time PCR
in this nanotiter plate format results in substantial savings in
reagents, measurement time and productivity. We demonstrate system
performance by measuring tissue-specific expression of kinase genes in
human heart and liver samples and transcriptional modulation by
small-molecule perturbation of the TNF-alpha signaling pathway in HUVEC
cells. Compared with the same assay in a 384-well
microplate, we measured equivalent rt-PCR performance with a 64-fold
reduction in assay volume, a 24-fold increase in analytical throughput
and a workflow compatible with standard microplate protocols in an
easy-to-use fomat.
Dynamic Arrays to
Measure Expression of Nucleic
Acids and Proteins
A dynamic array is
a biochip that employs integrated
channels and valves in a matrix architecture. This matrix architecture
is a breakthrough in efficiency because performing the same set of
reactions by hand or with a robot would require orders of magnitude
more sample, reagents, and pipetting steps.Dynamic
arrays have been introduced that handle
homogeneous or heterogeneous assays, such as real-time qPCR and ELISAs,
respectively. BioMark™ dynamic arrays for qPCR accept 48 samples and 48
primer/probe sets. The components are combined into 2,304 assays (48 x
48).The chip is ideal to validate expression for 48
genes on samples from many individuals. Projected throughput of future
chips is ~10,000 reactions. BioMark™ dynamic arrays for immunoassays
accept 48 samples and 18 antibody pairs and generate 1,728 assays.
Integrated valves prevent mixing between antibody pairs.Thus,
dynamic arrays prevent signal crosstalk and
eliminate the need to optimize antibody pairs for multiplexing in one
vessel, a requirement with other formats. Instrumentation automates the
loading of chips and analysis of results. Data produced on BioMark™
dynamic arrays for both applications will be presented, demonstrating a
sensitivity of detection equivalent to conventional microwell plates
while generating orders of magnitude higher throughput.
A Sequence Oriented
Comparison of Gene Expression
Measurements Across Different Hybridization-Based Technologies
Gene expression
microarrays have made a profound
impact in biomedical research. The diversity of platforms and
analytical methods has made comparison of data from multiple platforms
very challenging. The presentation will describe a framework for
comparisons across platforms and laboratories, where probe sequences
were utilized from each vendor to map of genes across platforms.
High-throughput
Measurement of Expression Signatures
Using Dynamic Arrays for qPCR and Immunoassays
Michael Lucero of
Fluidigm speaking at the European
Biomarkers Summit 2006
To purchase a DVD of all the presentations featured
at the European Biomarkers Summit, please go to the Select Biosciences
website.
RNAi
screening – advanced tools to accelerate translational research and
drug discovery
In
recent years, RNAi
screening using synthetic siRNA libraries has become a popular tool for
elucidating gene functional pathways and for target identification.
Find out about the essentials of RNAi screening and the latest tools
developed by QIAGEN to enable its broad application from Dr. Eric
Lader, QIAGEN’s Associate Director of RNAi research.
RNAi screening - advanced tools to accelerate
translational research and drug discovery.
Abnormal
expression
of microRNAs (miRNAs) in cancer implies that these small ~22-nucleotide
molecules play a role in oncogenesis, and therefore may comprise a
novel class of diagnostic and prognostic signatures. Here, we are
studying the global expression profiles of miRNAs in breast cancer and
adjacent non-malignant breast tissue in order to identify possible new
biomarkers for breast cancer
Biopsies from
primary
tumors and from the proximal tissue (1 cm and 5 cm from the border zone
of tumor) were collected from female patients (age 55-69) undergoing
surgery for invasive ductal carcinoma. Total RNA was extracted and
analyzed for global miRNA expression on a miRCURYTM LNA-based
microarray platform containing capture probes for over 400 miRNAs.
Our analysis of
miRNA
expression patterns from tumor and proximity tissue revealed numerous
differentially expressed miRNAs, including those reported to be
associated with breast cancer, such as let-7a/d/f, miR-125a/b, miR-21,
miR-32, and miR-136.
In addition, we
have
identified several miRNAs that have not previously been connected with
breast cancer. Some of these novel miRNA signatures could have
diagnostic and prognostic potential for breast cancer patients.
Several cases of
off-target gene silencing were identified in our siRNA library screens
(Lin X et.al. Nucleic Acids Research 33: 4527-35, 2005). However,
despite the complications of off-target gene silencing, we successfully
identified three cancer targets by screening an siRNA library against
the “druggable genome” using a cell-death assay.
In addition, in an
siRNA-based synthetic lethal screen, we found that knockdown of
survivin led to the selective killing of K-Ras mutant cells. We also
explored RNAi-based methodology for target validation in animal models.
We developed a
tightly regulated shRNA expression system (Lin X. et.al. FEBS letter,
577: 376-80, 2004) and used this system to prepare stable tumor cell
lines that conditionally expressed an shRNA for HIF-1a. These tumor
cells were implanted in mice to form tumors that served as a versatile
tool for studying the effects of inhibiting HIF-1 in vivo (Li L et.al.
Cancer Research, 65: 7249-58, 2005).
Finally, we
investigated several methods for the creation of germline knockdown
animals. By modifying the standard methods, we successfully increased
the transgenic frequency and F1 transmission rate and created
tyrosinase knockdown mice with a lighter-coat-color phenotype that can
be stably transmitted to the next generation.
Welcome to
the OpenGenomics Peer Science series, where your colleagues
discuss their latest findings in Genomics research. With technical
webcasts from your peers, podcasts providing distilled takes on
research breakthroughs, and the latest in published papers, Open
Genomics is dedicated to providing fresh perspectives on pressing
research questions. Check here regularly for the latest developments in
Genomics.
Each webcast
contains a short scientific
talk on a specific area of
research, presented by the researcher. These webcasts are 15-20 minutes
long and are available on demand.
Each Podcast is
developed as an
informational brief highlighting
innovative approaches to fundamental research questions, available as
an RSS feed to your IPod®, or available for listening on your
computer.
These weekly
podcasts
feature interviews with leading biotech researchers, newsmakers, and
thought leaders. Topics revolve around the critical scientific and
business issues that impact the global biotechnology industry,
beginning with new discoveries in the lab and then moving onto R &
D, biomanufacturing, and final product commercialization. Trends, novel
technologies, opinions, recent developments, how-to advice, and much
more are discussed in our podcasts in a lively, to-the-point, and
informative style. New every Thursday, you can listen right from the
GEN website or you can subscribe using the button below to have it
download each week automatically to your iPod or mp3 player!
THE INVENTOR
OF PCR - GEN's
Editor-in-Chief John Sterling interviews the Nobel Prize winning
inventor of PCR, Dr. Kary Mullis.
(2/15/2007) sponsored by: Eppendorf
RNAi
TECHNOLOGY
- Richard Jorgensen, Ph.D., from the University of Arizona
(3/22/2007) sponsored by: Sigma-Aldrich
qPCR AND
LATE-PCR, AN
ADVANCED TECHNIQUE FOR DNA AMPLIFICATION - Lawrence Wangh,
Ph.D., from Brandeis University (2/22/2007) sponsored
by: Eppendorf
PROBE-BASED,
REAL-TIME
QUANTITATIVE PCR ASSAY DESIGN AND APPLICATIONS - Gregory
L. Shipley, Ph.D., Director, Quantitative Genomics Core Laboratory, The
University of Texas Health Science Center-Houston.
(2/1/2007) sponsored by: Eppendorf
for Windows (.wmv)
for Macintosh (.mov)
for
Printing (.pdf)