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Sars-Cov-2
Quantification
-- MIQE, Pitfalls and Solutions
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Title |
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References
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JIM HUGGETT
PhD, Science Fellow, UK
National Measurement Laboratory, Nucleic Acid
Metrology, LGC, Teddington, UK
visiting Professor, Analytical Microbiology,
University of Surrey, UK
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How to approach diagnosing the next
pandemic. A lesson from Covid-19.
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CARL WITTWER
Professor, Department
of Pathology, University of Utah, US
Adjunct Professor,
Biomedical Engineering, University of Utah, US
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At-home PCR
testing for SARS-CoV-2 |
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MAURICE JB van
den HOFF
PhD, AmsterdamUMC, Location AMC, Department
Medical Biology, Meibergdreef 15, 1105AZ
Amsterdam, The Netherlands
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Reliable
quantification of Sars-Cov-2 in standard
clinical samples is not possible |
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CORINNA
HOLETSCHEK
Environmental Medicine, Faculty of Medicine,
University of Augsburg, Germany; Institute of
Environmental Medicine, Helmholtz Center Munich,
Germany; Chair of Environmental Medicine,
Technical University of Munich, Germany
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Sars-CoV-2 RNA
vs Antigen Kinetics in Different Compartments |
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invited by
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AGNES MEIDERT
Department of
Anesthesiology, Biomarker Research Lab, LMU
University Hospital Grosshadern,
Ludwig-Maximilians-University Munich, Munich,
Germany
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Extracellular
vesicles in Covid-19 pneumonia and progression
to acute respiratory failure
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Spatial-Transcriptomics
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RAFFAELE A.
CALOGERO
Molecular Biotechnology Center, University of
Torino, Torino, Italy
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Limits and
strength of Visium spatial transcriptomics
platform
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MATHIAS HOLPERT
PhD, Principle Product Application Scientist,
NanoString Technologies, Inc.
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Spatial
transcriptomics – studies on the single cell
and functional level of tissue |
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AHMAD AL ALWASH
Predoctoral Fellow in Jan’s Korbel lab at the
Genome Biology Unit, EMBL, Heidelberg, Germany
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Spatial
Multi-omics Analysis of The Tumor
Microenvironment In Colon Cancers
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MARC REUDELSTERZ
Vizgen Inc, USA
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Molecular
Atlassing with MERSCOPE Reveals the Spatial
Organization of Mouse and Human Tissues
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LUKAS VALIHRACH
Senior Scientist, Laboratory of Gene Expression,
Institute of Biotechnology CAS @ BIOCEV,
CzechBIO, The Technology Centre CAS, Czech
Republic
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Patiotemporal
dynamics of ischemic brain injury resolved at
single-cell level
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Multi-Omics
Biomarkers
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PIETER MESTDAGH
CellCarta,
Technologiepark 3, B-9052 Zwijnaarde,
Belgium
Prof. at Center for Medical
Genetics, University of Ghent,
Belgium
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Leveraging tumor
RNA-sequencing data to quantify TMB, MSI and
TIL for immune checkpoint inhibition therapy
response prediction
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ROBERT
LOEWE
CEO, GeneSurge GmbH, Munich, Germany
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Real world
multi-omics not lost in clinical translation
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MARKUS LIST
Head of "Big Data in BioMedicine", Chair of
Experimental Bioinformatics, TUM School of Life
Sciences, Technical University of Munich,
Germany
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Competing
endogenous RNAs as biomarkers of miRNA
activity |
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PHILIPP BECKER
PhD, Singleron Biotechnologies, Cologne, Germany
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Multi-verse
in a nutshell: Advanced single cell
multi-omics technologies
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SARANTIS
CHLAMYDAS
Phd, Scientific Director, MultiOmics, Olink
Proteomics, Uppsala, Sweden
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Novel NGS-based
proteomics revolutionizes biomarker
identification in liquid biopsies
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Liquid Biopsy
& Circulating Nucleic Acids
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KLAUS PANTEL
Director of the Department of Tumor Biology,
University Medical Center Hamburg-Eppendorf,
University of Hamburg, Germany
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Liquid Biopsy --
From Discovery to Clinical Implementation
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JO VANDESOMPELE
Professor at Department of
Biomolecular Medicine, Faculty of Medicine and
Health Sciences, University of Ghent, Belgium
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Performance of RNA purification kits
and blood collection tubes in the Extracellular
RNA Quality Control (exRNAQC) study
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ADAM S CORNER
PhD, Market
Development Manager, EMEA & APAC, Digital
Biology Group, Bio-Rad Laboratories, USA
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Maximizing
metastasis insights: Using an integrated
workflow approach to achieve efficient CTC
enrichment with targeted enumeration for
versatile downstream applications
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STEFAN HOLDENRIEDER
Prof, MD, Director of Institute of Laboratory
Medicine, German Heart Centre Munich, Technical
University of Munich, Germany
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New perspectives
for liquid profiling of circulating nucleic
acids in clinical diagnostics
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NIELS
PALLISGAARD
Professor, Department of Science and
Environment, Molecular and Medical Biology,
Sjællands Universitetshospital Roskilde,
Patologiafdelingen, Roskilde, Denmark
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Systemic
Alterations in the Systemic Inflammatory
Response and Circulating Cell-free DNA in
Relation to Stent Placement
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invited by
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STEFANIA
CRUCITTA
PhD, Clinical
Pharmacology and Pharmacogenetics Unit,
Department of Clinical and Experimental
Medicine, University of Pisa, Pisa, Italy
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Comparison Of
Two Digital PCR Systems For The Analysis Of
Liquid Biopsy Samples Of Patients Affected By
Lung And Colorectal Cancer
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invited by
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ANDERS STAHLBERG
Prof. and Senior Scientist, Sahlgrenska Cancer
Center, University of Gothenburg, Sweden
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Digital
sequencing using liquid biopsies: clinical and
technical aspects
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| Digital-PCR |
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JIM HUGGETT
PhD, Science
Fellow, UK National Measurement Laboratory,
Nucleic Acid Metrology, LGC, Teddington, UK
visiting Professor, Analytical Microbiology,
University of Surrey, UK
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10 years Of
digital MIQE; What have we learned?
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MATTHIJS VYNCK
PhD, Department of
Morphology, Imaging, Orthopedics, Rehabilitation
and Nutrition, Faculty of Veterinary Medicine,
Ghent University, Belgium
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Achieve
high-level multiplexing using 6-color digital
PCR with robust assay designs and partition
classification
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MEGAN CLEVELAND
NIST, Monrovia, MD, USA
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Accuracy of
Digital Polymerase Chain Reaction
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GARY PESTANO
PhD, Chief
Development Officer, Biodesix Inc., Boulder,
Colorado, USA
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Development of
Advanced Droplet Digital PCR Applications for
the Clinical Lab |
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invited by
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VALERIE TALY
PhD, group leader, INSERM Université Paris
Descartes UMRS1147, CNRS SNC 5014, Equipe
Labellisée Ligue nationale contre le cancer
(LNCC), France
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Digital PCR for
the follow-up of patient with cancer
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ROBERT SJÖBACK
Head of R&D,
TATAA Biocenter AB, Göteborg, Sweden
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Design and
Validation of Precision dPCR assays
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MicroGenomics:
EV & Single-Cells Diagnostics
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MIKAEL KUBISTA
Prof. of Biotechnology, BTU, Czech
Academy of Sciences & TATAA Biocenter,
Sweden
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Applications of Two-Tailed PCR
for Precision Diagnostics
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PAMELA PINZANI
Associate Professor of Clinical Biochemistry
Clinical and Molecular Biochemistry Laboratory
Careggi University Hospital, Department of
Biomedical, Experimental and Clinical Sciences
University of Florence, Italy
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Molecular
characterization of single Circulating Tumor
Cells -- challenges and promise for
translation to the clinic
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THOMAS KRONEIS
Division of Cell Biology, Histology &
Embryology, Gottfried Schatz Research Center for
Cell Signaling, Metabolism and Aging, Medical
University of Graz, Austria
Total Quality Management, Center for Biomarker
Research in Medicine GmbH, Graz, Austria
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On the
analysis of microchimerism
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RADEK SINDELKA
PhD, Gene Expression Laboratory, IBT, Institute
of Biotechnology CAS, Vestec, Czech Republic
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Healing as a key
phase of regeneration studied by bulk,
single-cell and spatial transcriptomic
analyses
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Non-coding RNAs
microRNA, small RNAs, tRFs, piRNAs, lnc-RNAs
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MICHAEL W. PFAFFL
Prof. of Molecular Physiology, Animal Physiology
& Immunology, TUM School of Life Sciences,
Freising Weihenstephan, Germany
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A
development and analysis pipeline for microRNA
biomarker signatures in molecular diagnostics
based on circulating EVs
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ERIC JAMES DE BONY
DE LAVERGNE
Center for Medical Genetics Ghent, Ghent
University, Ghent, Belgium, Biogazelle,
Zwijnaarde, Belgium
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High-throughput
CRISPR-interference screening platform to
study lncRNA functions through serial cellular
and molecular phenotypin
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Next Generation
Sequencing
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STEFAN FILGES
CTO, Simsen Diagnostics, Sahlgrenska Science
Park, Gothenburg, Sweden
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Tumour-guided
Personalised Sequencing Assays For ctDNA
Detection
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KLAUS NEUHAUS
PhD, Head of Core
Facility Microbiome, ZIEL Institute for Food
& Health, Technische Universität München,
Germany
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Quantification
with Spike DNA and Common Pitfalls of 16S rRNA
Gene Amplicon Data Analysis in Microbiome
Research
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SUBRATA PANJA
Quantabio, 100 Cummings Center Suite 407J,
Beverly, MA, USA |
Significant
Improvements To PCR Amplification Enables
Better Performance For A Multitude Of Next
Generation Sequencing Applications: Library
Amplification With Fast, Processive,
Higher-Fidelity And Inhibitor Tolerant PCR
Master Mixes In The New Era Of NGS
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NISH KUMAR
Bio-Rad Laboratories
Inc., Hercules CA, USA
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Simultaneous
Interrogation of Small RNAs and Long RNAs from
archival breast cancer FFPE samples using Next
Generation Sequencing (NGS)
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CELESTE
LETELLIER
Applications, EMEA, Standard Biotools, Lyon, France
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Setting the new
benchmarks in automated and cost-effective
qPCR and NGS assays -- Meet the
Biomark X and Juno
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KRYSTYNA NAHLIK
PhD, LGC Clinical Diagnostics,
Wetzlar, Germany
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Essential
Factors Affecting the Assessment of Homologous
Recombination Deficiency by NGS
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invited by
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Data Analysis, Algorithms and more
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TOBIAS
ÖSTERLUND
Bioinformatician, Gothenburg University and
Sahlgrenska University Hospital, Sweden
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Bioinformatics
tools for ultra-sensitive sequencing data
using unique molecular identifiers
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DAVID LANGENBERGER
CEO, PhD, ecSeq Bioinformatics GmbH, Leipzig,
Germany
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Gene
Fusion Detection - more complex than you might
assume
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AMIN FOROOTAN
CEO, MultiD Göteborg, Sweden
MIKAEL
KUBISTA
CEO, TATAA Biocenter,
Göteborg, Sweden
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Genex For The Analysis Of QPCR
And DPCR Data In Regulated Environments
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Advanced
Molecular
Dx
in Life Science
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STEPHEN A. BUSTIN
Prof. of Molecular Medicine, Postgraduate
Medical Institute, Faculty of Health, Social
Care & Education, Anglia Ruskin University,
UK
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RT-ePCR:
A paradigm shift for molecular diagnostics
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VANESSA GEBAUER
PhD, Head of
Technical Operations, IMGM Laboratories GmbH,
Munich, Germany
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Putting Ideas
Into Action - How IMGM Can Accelerate Your
Research
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CHRISTIAN SIGL
CEO, capsitec GmbH,
Germany |
Programmable
Antiviral Platform Based On DNA Nanotechnology
– The VIRUS TRAP
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MATTHIEU LEWIS
PhD, Senior Market Strategy Manager,
Molecular Diagnostics at Takara Bio Europe,
St.-Germain-en-Laye, Île-de-France, France
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How Research
Tools and Enzymes Became Fundamental
Components of Molecular Diagnostic Solutions |
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ABEL BRONKHORST
PhD, Institute of Laboratory Medicine, German
Heart Centre Munich, Technical University of
Munich, Germany
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Biological,
structural and functional aspects of
circulating nucleic acids
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ANNE LOUISE
WYLLIE
Research Scientist in Epidemiology (Microbial
Diseases), Yale School of Public Health, New
Haven, USA
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Simplified
Testing for Public Health Impact
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PATRICK GÜRTLER
PhD, Bavarian Health and Food Safety Authority,
Molecular Biology Unit, Bayerisches Landesamt
für Gesundheit und Lebensmittelsicherheit (LGL),
Oberschleissheim, Germany
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Detection of mutations generated by
genome editing - activities of the § 28b GenTG
working group
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ERIK WENDLANDT
Manager of Core and
qPCR Applications Development, IDT, USA
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Novel
identification in diverse sample types through
direct amplification
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RASMUS KOEFOED
PETERSEN
R&D, Pentabase, Odense, Denmark
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A novel qPCR
technology for direct quantification of
methylations in untreated DNA
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BRIGITTA SAUL
PhD, Promega Corporation, Madison, WI, USA
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Design of qPCR
assays including standards and controls for
pathogen testing in wastewater
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MIQE
& Quality Management &
Standardisation Strategies in Molecular
Diagnostics
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JAN RUIJTER
PhD, Department of Medical Biology, Amsterdam
University Medical Center (UMC), The Netherlands
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One more time: do not ignore the
amplification efficiency! |
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JAN HELLEMANS
CTO, CellCarta,
Technologiepark 3, B-9052 Zwijnaarde, Belgium
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Guidance for the
Validation of qPCR/dPCR Assays in Clinical
Research
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JENS BJÖKMAN
President, Head of Commissioned Services,
TATAA Biocenter AB, Göteborg, Sweden
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Lifting a nucleic acid service
lab to GLP compliance
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DAVE SCHUSTER
R&D, Quantabio,
Beverly, MA, USA
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QScript Ultra
RT: A Next-Generation Reverse Transcriptase
Platform To Break Cold-Chain Barriers And
Democratize State Of The Art RNA
Quantification Tools
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ANDREAS
UNTERGASSER
Zentrum für Molekulare Biologie der
Universität Heidelberg
Im Neuenheimer Feld 329, D-69120 Heidelberg,
Germany
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Absolute
quantification of estimated copy number
without a standard curve
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