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Sars-Cov-2
Quantification
-- MIQE, Pitfalls and Solutions
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References
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JIM HUGGETT
PhD, Science Fellow, UK
National Measurement Laboratory, Nucleic Acid
Metrology, LGC, Teddington, UK
visiting Professor, Analytical Microbiology,
University of Surrey, UK
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The need for reproducible and
standardized metrics for SARS-CoV-2 -- What we
learned from COVID-19
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CARL WITTWER
Professor, Department
of Pathology, University of Utah, US
Adjunct Professor,
Biomedical Engineering, University of Utah, US
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At-home PCR
testing for SARS-CoV-2 |
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AGNES MEIDERT
& GUSTAV SCHELLING
Department of
Anesthesiology, Biomarker Research Lab, LMU
University Hospital Grosshadern,
Ludwig-Maximilians-University Munich, Munich,
Germany
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OLFERT LANDT
Founder TIB MOLBIOL,
Berlin, Germany
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The learning
lession from Covid diagnostics |
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Spatial-Transcriptomics
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RAFFAELE A.
CALOGERO
Molecular Biotechnology Center, University of
Torino, Torino, Italy
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Limits and
strength of Visium spatial transcriptomics
platform
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LUKAS VALIHRACH
Senior Scientist, Laboratory of Gene Expression,
Institute of Biotechnology CAS @ BIOCEV,
CzechBIO, The Technology Centre CAS, Czech
Republic
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Patiotemporal
dynamics of ischemic brain injury resolved at
single-cell level
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Multi-Omics
Biomarkers
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PIETER MESTDAGH
Prof. at Center for
Medical Genetics, Biogazelle,
Technologiepark 3, B-9052 Zwijnaarde, Belgium
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Leveraging tumor
RNA-sequencing data to quantify TMB, MSI and
TIL for immune checkpoint inhibition therapy
response prediction
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ROBERT
LOEWE
CEO, GeneSurge GmbH, Munich, Germany
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Real world
multi-omics not lost in clinical translation
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MARKUS LIST
Head of "Big Data in BioMedicine", Chair of
Experimental Bioinformatics, TUM School of Life
Sciences, Technical University of Munich,
Germany
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Competing
endogenous RNAs as biomarkers of miRNA
activity |
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Liquid Biopsy
& Circulating Nucleic Acids
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KLAUS PANTEL
Director of the Department of Tumor Biology,
University Medical Center Hamburg-Eppendorf,
University of Hamburg, Germany
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Liquid Biopsy --
From Discovery to Clinical Implementation
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JO VANDESOMPELE
Prof. at Center for Medical Genetics Ghent,
Ghent University, Ghent, Belgium, Biogazelle,
Zwijnaarde, Belgium
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Performance of RNA purification kits
and blood collection tubes in the Extracellular
RNA Quality Control (exRNAQC) study
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STEFAN HOLDENRIEDER
Prof, MD, Director of Institute of Laboratory
Medicine, German Heart Centre Munich, Technical
University of Munich, Germany
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New perspectives
for liquid profiling of circulating nucleic
acids in clinical diagnostics
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ANDERS STAHLBERG
Prof. and Senior Scientist, Sahlgrenska Cancer
Center, University of Gothenburg, Sweden
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A
generic, patient-specific and ultrasensitive
sequencing approach for monitoring treatment
efficacy using liquid biopsies
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| Digital-PCR |
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JIM HUGGETT
PhD, Science
Fellow, UK National Measurement Laboratory,
Nucleic Acid Metrology, LGC, Teddington, UK
visiting Professor, Analytical Microbiology,
University of Surrey, UK
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10 years of
digital MIQE, what have we learned?
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MicroGenomics:
EV & Single-Cells Diagnostics
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MIKAEL KUBISTA
Prof. of Biotechnology, BTU, Czech
Academy of Sciences & TATAA Biocenter,
Sweden
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Applications of Two-Tailed PCR
for Precision Diagnostics
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PAMELA PINZANI
Associate Professor of Clinical Biochemistry
Clinical and Molecular Biochemistry Laboratory
Careggi University Hospital, Department of
Biomedical, Experimental and Clinical Sciences
University of Florence, Italy
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Molecular
characterization of single Circulating Tumor
Cells -- challenges and promise for
translation to the clinic
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THOMAS KRONEIS
Division of Cell Biology, Histology &
Embryology, Gottfried Schatz Research Center for
Cell Signaling, Metabolism and Aging, Medical
University of Graz, Austria
Total Quality Management, Center for Biomarker
Research in Medicine GmbH, Graz, Austria
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On the
analysis of microchimerism
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VLADIMIR BENES
PhD, Head of Gene Core Facility @ EMBL,
Heidelberg, Germany
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RADEK SINDELKA
PhD, Gene Expression Laboratory, IBT, Institute
of Biotechnology CAS, Vestec, Czech Republic
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Healing as a key
phase of regeneration studied by bulk,
single-cell and spatial transcriptomic
analyses
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Non-coding RNAs
-- microRNA, small RNAs, tsRNA, lnc-RNAs
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MICHAEL W. PFAFFL
Prof. of Molecular Physiology, Animal Physiology
& Immunology, TUM School of Life Sciences,
Freising Weihenstephan, Germany
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A
development and analysis pipeline for microRNA
biomarker signatures in clinical and molecular
diagnostics based on circulating EVs
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ERIC JAMES DE BONY
DE LAVERGNE
Center for Medical Genetics Ghent, Ghent
University, Ghent, Belgium, Biogazelle,
Zwijnaarde, Belgium
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High-throughput
CRISPR-interference screening platform to
study lncRNA functions through serial cellular
and molecular phenotypin
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ROBERT SJÖBACK
Head of R&D,
TATAA Biocenter AB, Göteborg, Sweden
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Design and
optimization of Two-Tailed PCR assays for
microRNA and sequence variant detection
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Next
Generation Sequencing
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GUSTAV JOHANSSON
CEO and Co-founder, Simsen Diagnostics,
Sahlgrenska Science Park, Gothenburg, Sweden
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Ultrasensitive
Immune Repertoire Sequencing Using Unique
Molecular Identifiers
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Data Analysis, Algorithms and more
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DAVID LANGENBERGER
CEO, Phd, ecSeq Bioinformatics GmbH, Leipzig,
Germany
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Gene
Fusion Detection - more complex than you might
assume
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AMIN FOROOTAN
MultiD Göteborg, Sweden
MIKAEL
KUBISTA
TATAA Biocenter, Göteborg,
Sweden
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Analyzing and validating gene
expression data in GenEx |
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Advanced
Molecular
Dx
in Life Science
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STEPHEN A. BUSTIN
Prof. of Molecular Medicine, Postgraduate
Medical Institute, Faculty of Health, Social
Care & Education, Anglia Ruskin University,
UK
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RT-ePCR:
A paradigm shift for molecular diagnostics
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JORDAN MOORE
Director, Applications, EMEA, Fluidigm / Standard
Biotools
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Setting the new
benchmarks in automated and cost-effective
qPCR and NGS assays
Meet the Biomark X and Juno
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ABEL BRONKHORST
PhD, Institute of Laboratory Medicine, German
Heart Centre Munich, Technical University of
Munich, Germany
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Biological,
structural and functional aspects of
circulating nucleic acids
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MIKAEL KUBISTA
Prof. of Biotechnology, BTU, Czech
Academy of Sciences & TATAA Biocenter,
Sweden
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Applications of Two-Tailed PCR
for Precision Diagnostics
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PATRICK GÜRTLER
PhD, Bavarian Health and Food Safety Authority,
Molecular Biology Unit, Bayerisches Landesamt
für Gesundheit und Lebensmittelsicherheit (LGL),
Oberschleissheim, Germany
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Detection of mutations generated by
genome editing - activities of the § 28b GenTG
working group
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MIQE
& Quality Management &
Standardisation Strategies in Molecular
Diagnostics
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CARL WITTWER
Professor, Department
of Pathology, University of Utah, US
Adjunct Professor,
Biomedical Engineering, University of Utah, US
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At-home PCR
testing for SARS-CoV-2 |
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JAN HELLEMANS
CTO, Biogazelle,
Technologiepark 3, B-9052 Zwijnaarde, Belgium
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Assay
validation: the minimum for research purposes
or in depth for clinical trials
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JENS BJÖKMAN
President, Head of Commissioned Services,
TATAA Biocenter AB, Göteborg, Sweden
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Lifting a nucleic acid service
lab to GLP compliance
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STEFAN RÖDIGER
Faculty of Natural Sciences, Brandenburg
University of Technology Cottbus-Senftenberg,
Senftenberg, Germany
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JAN RUIJTER
PhD, Department of Medical Biology, Amsterdam
University Medical Center (UMC), The Netherlands
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One more time: do not ignore the
amplification efficiency! |
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