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With over 50 years of dedication to quality and customer service, Bio-Rad Laboratories has a solution for all your amplification needs. From amplification reagents to real-time PCR and digital PCR instruments, optical-quality PCR plates and reaction vessels, Bio-Rad has the products and experience to ensure success.

Droplet Digital™ PCR

Bio-Rad’s Droplet Digital PCR (ddPCR™) system provides absolute quantification of target DNA or RNA molecules with unmatched sensitivity and precision for a wide range of applications.

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QX200™ Droplet Digital PCR System

QX200 Droplet Digital PCR System

The QX200 Droplet Digital PCR system is Bio-Rad’s second-generation ddPCR system. It allows absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Benefits of the QX200 Droplet Digital PCR system

The QX200 droplet generator is used to partition ddPCR reaction mix into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 droplet reader. Droplets pass by a two-color optical detection system in a serial manner. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning. Up to 96 samples can be processed per run.

A Versatile and Scalable Workflow

  • 1Prepare ddPCR reaction mix

    • Combine DNA sample, primers, and/or probes with one of Bio-Rad’s ddPCR supermixes
    • Fully validated PrimePCR ddPCR assays can be used
  • 2Generate droplets

    • Load the ddPCR reaction mix into the wells of a droplet generator cartridge
    • 8 x 20,000 droplets are generated from each run in the QX200 droplet generator
    • Target DNA ( ) and background DNA ( ) are randomly distributed in droplets
  • 3Perform PCR with EvaGreen or hydrolysis probes

    • Transfer the droplets to a 96-well PCR plate and seal the plate
    • Run the PCR protocol
  • 4Read and analyze results

    • After PCR, load the 96-well PCR plate into the QX200 droplet reader
    • Positive and negative droplets in each sample are read
    • Analyze concentrations with QuantaSoft™ software

Applications of the QX200 Droplet Digital PCR System

Specifications for the QX200 Droplet Digital PCR system

Starting sample size, μl 20
QX200 droplet generator capacity, samples/cartridge 8
Droplets per sample 20,000
Detection channels FAM, VIC/HEX
Linear dynamic range 5 orders of magnitude
Precision +10%
Droplets per 96-well plate, million 1.4

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Digital PCR Reagents and Kits

Ready-to-use 2X supermixes that are suitable for use in ddPCR assays. Tested for reliable amplification over a wide dynamic range of template input and template type (genomic DNA, cDNA, and plasmid DNA).

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Probe Assays for Droplet Digital PCR

Droplet Digital PCR (ddPCR) technology provides an absolute measure of target DNA molecules with unrivaled accuracy, precision, and sensitivity, accelerating discovery and enabling new research strategies for the study of inherited disorders, cancer, and infectious disease. Probe assays for the QX100™ and QX200 Droplet Digital PCR systems provide single-copy PCR resolution without a standard curve. ddPCR probe assays are available with a FAM or HEX fluorophore. We offer:

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Real-Time PCR Instrumentation

Bio-Rad’s real-time PCR amplification systems combine thermal cyclers with optical reaction modules for singleplex and multiplex detection of fluorophores. Bio-Rad’s real-time PCR detection systems range from economical two-target detection to sophisticated five-target detection systems. All Bio-Rad real-time detection platforms offer reliable gradient-enabled thermal cyclers for rapid assay optimization.

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qPCR Systems

  CFX96 Touch Real-Time PCR Detection System
CFX96 Touch™
Real-Time PCR
Detection System
CFX384 Touch Real-Time PCR Detection System
CFX384 Touch™
Real-Time PCR
Detection System
CFX Connect Real-Time PCR Detection System
CFX Connect™
Real-Time PCR
Detection System
CFX96 Touch Deep Well Real-Time PCR Detection System
CFX96 Touch Deep Well
Real-Time PCR
Detection System
Features
Powerful and precise, full-featured real-time PCR instrument that supports up to 5-channel multiplexing.Learn More
Flexible system that can accommodate high-throughput real-time PCR in 384-well plates, with up to 4-channel multiplexing.Learn More
Excellent thermal performance for two-target multiplex PCR and routine detection of single fluorophore experiments.Learn More
Industry-leading technology for large-volume reactions in real-time PCR applications.Learn More
Exitation range 450–684 nm 450–650 nm 450–535 nm 450–684 nm
Light source 6 LEDs in optics shuttle 5 LEDs in optics shuttle 3 LEDs in optics shuttle 6 LEDs in optics shuttle
Optical detection 6 photobodies 5 photobodies 3 photobodies 6 photobodies
Detection range 515–730 nm 515–690 nm 515–580 nm 515–730 nm
Multiplex capability Up to 5 targets Up to 4 targets Up to 2 targets Up to 5 targets
Gradient range 3–100°C 3–100°C 3–100°C 3–100°C
Maximum gradient span 24°C 24°C 24°C 24°C
Maximum ramp rate 5°C/sec 2.5°C/sec 24°C 24°C
Dimensions (W x D x H) 33 x 46 x 36 cm 33 x 46 x 36 cm 33 x 46 x 36 cm 33 x 46 x 36 cm
Weight 21 kg 21 kg 21 kg 21 kg

CFX Automation System for Real-Time PCR

The CFX automation system works with the CFX96 Touch and CFX384 Touch real-time PCR detection systems to enable walk-away, high-throughput operation in a simplified format that does not compromise precision. The plug-and-go benchtop plate handler has the capacity to load up to twenty 384-well PCR plates at a time.

This system facilitates the automation of workflows, generation of large volumes of data, and rapid data analysis. It is ideally suited to meet the high-throughput requirements of today’s drug discovery workflows, letting you process up to 7,680 samples in a single run.

The CFX automation system makes it easy to:

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Amplification Software

CFX Maestro Software

CFX Maestro Software provides experiment setup and data analysis capabilities for the MiniOpticon™, CFX Connect, CFX96 Touch and CFX384 Touch real-time PCR detection systems.

Available Editions: English | Chinese | Russian

CFX Maestro Software, Secure Edition

The new CFX Maestro Software description: CFX Meastro software provides experiment setup and data analysis capabilities for the CFX Connect, CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch real-time PCR detection systems. Maestro also includes capabilities for some statistical analyses and publication-quality graphing.

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Precision Melt Analysis Software

Precision Melt Analysis software imports and analyzes data files generated from the CFX Connect, CFX96 Touch or CFX384 Touch real-time PCR detection system to genotype samples based on the thermal denaturation profiles of double-stranded DNA. The software can be used for a variety of genotyping applications, including scanning for new gene variants, screening DNA samples for single nucleotide polymorphism (SNP), identifying insertions/deletions or other unknown mutations, and determining the percentage of methylated DNA in unknown samples.

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PCR primers, probes, and panels

Assays and Panels for Real-Time PCR

Our real-time PCR assays were designed in collaboration with leading experts in real-time PCR research at Biogazellle. Every PCR primer pair has been experimentally validated to ensure optimal assay performance. We offer:

All real-time PCR assays and panels can be used with CFX real-time PCR detection systems and data analysis software, streamlining PCR data collection and analysis.

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Real-Time PCR Supermixes and Kits

Bio-Rad’s supermixes can make any qPCR experiment easier, faster, and more effective. We offer ready-to-use supermixes designed to deliver robust and reliable performance for a wide range of applications on any qPCR platform.

Use our interactive selection tool to find the right products for your needs. Filter by application, technique, detection chemistry, or product-specific features to easily compare reagents.

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SsoAdvanced™ Universal SYBR® Green Supermix

SsoAdvanced Universal SYBR Green Supermix

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SsoAdvanced™ Universal Probes Supermix

SsoAdvanced Universal Probes Supermix

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SsoFast™ EvaGreen® Supermix

SsoFast EvaGreen Supermix

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iTaq™ Universal SYBR® Green Supermix

iTaq Universal SYBR Green Supermix

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iTaq™ Universal Probes Supermix

iTaq Universal Probes Supermix

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iQ™ Multiplex Powermix

iQ Multiplex

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Precision Melt Supermix

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Reverse Transcription Reagents for RT-PCR

iScript™ reverse transcription products are formulated for efficient and sensitive reverse transcription over a wide linear dynamic range of input RNA for real-time qPCR.

Reverse Transcription (for 2-step RT-qPCR)

iScript™ Advanced cDNA Synthesis Kit for RT-qPCR

iScript Advanced cDNA Synthesis Kit for RT-qPCR

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iScript™ Reverse Transcription Supermix for RT-qPCR

iScript Reverse Transcription Supermixfor RT-qPCR

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iScript™ cDNA Synthesis Kit

iScript cDNA Synthesis Kit

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iScript™ Select cDNA Synthesis Kit

iScript Select cDNA Synthesis Kit

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One-Step RT-PCR Kits

iTaq™ Universal SYBR® Green One-Step Kit

iTaq Universal SYBR® Green One-Step Kit

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iTaq™ Universal Probes One-Step Kit

iTaq Universal Probes One-Step Kit

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RT-PCR Directly from Cells

iScript™ RT-qPCR Sample Preparation Reagent

iScript RT-qPCR Sample Preparation Reagent

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Polymerases & Accessory Products for PCR/qPCR

Core reagents form the basis for many of Bio-Rad’s supermixes and kits for PCR and real-time PCR applications. Reagents include DNA polymerases, fluorescent dyes, dNTP mix, and reaction buffers.

Thermostable and High-Fidelity DNA Polymerases

iProof™ High-Fidelity DNA Polymerase

iProof High-Fidelity DNA Polymerase

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iTaq™ DNA Polymerase

iTaq DNA Polymerase

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Accessory Products for PCR/qPCR

dNTP Mix

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Sample Purification

Bio-Rad offers a complete line of kits and reagents for fast and effective extraction and purification of RNA, DNA, and PCR products.

RNA Isolation

Aurum™ Total RNA Mini Kit

Aurum Total RNA Mini Kit

Aurum Total RNA Kits are a family of isolation kits that provide a high yield of intact RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as plant and animal tissues.

Aurum Total RNA Fatty and Fibrous Tissue Kit

Aurum Total RNA Isolation Products come in:

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PureZOL™ RNA Isolation Reagent

PureZOL RNA Isolation Reagent

The ready-to-use PureZOL RNA isolation reagent is a versatile and efficient means of isolating high yields of RNA from a variety of sources, including cultured cell, animal and plant tissue, yeast, virus, and bacteria samples.

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DNA Isolation

InstaGene™ Matrix

Instagene Matrix

InstaGene matrix, made with a specially formulated 6% w/v Chelex® resin, makes DNA sample preparation fast, easy, and cost-effective, providing PCR-quality template DNA in less than an hour.

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Quantum Prep® Plasmid Kits

Quantum Prep Plasmid Kits

The consistently high yields achieved with Quantum Prep plasmid kits are due to the high-affinity binding of DNA to the unique Quantum Prep DNA binding matrix.

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DNA Cleanup

Freeze N’ Squeeze DNA Gel Extraction Spin Columns

Freeze ’N Squeeze™ DNA Gel Extraction Spin Columns

Freeze ’N Squeeze DNA gel extraction spin columns provide a quick and effective means to purify double-stranded DNA fragments from TAE- or TBE-buffered agarose gels.

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