Droplet Digital PCR Systems
Bio-Rad’s Droplet Digital PCR Systems provide ultrasensitive
and absolute nucleic acid quantification.
A high-throughput, multiplexed, and integrated
Droplet Digital PCR system
For high-throughput analysis
For analyzing up to 96 samples
Benefits of Droplet Digital PCR Technology
- Most precise and sensitive digital PCR solution for a wide
variety of applications
- Flexible digital PCR chemistry – optimized for hydrolysis
probes and EvaGreen assays
- Flexible assay setup – scalable for high sensitivity or high
throughput
- Simple and easy-to-use workflow with 96-sample throughput
- Droplet partitioning reduces bias from amplification
efficiency and PCR inhibitors
- Convenient assay design – standard curves are not required
-
- Combine DNA sample, primers, and/or probes
with one of Bio-Rad’s ddPCR supermixes
- Fully validated PrimePCR ddPCR assays can be
used
-
- Load the ddPCR reaction mix into the wells
of a droplet generator cartridge
- 8 x 20,000 droplets are generated from each
run in the QX200 droplet generator
- Target DNA ( )
and background DNA ( )
are randomly distributed in droplets
-
- Transfer the droplets to a 96-well PCR plate
and seal the plate
- Run the PCR protocol
-
- After PCR, load the 96-well PCR plate into
the QX200 droplet reader
- Positive and negative droplets in each
sample are read
- Analyze concentrations with QuantaSoft™
software
Applications of Droplet Digital PCR Technology
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Digital PCR Supermixes
Ready-to-use 2X supermixes that are suitable for use in ddPCR
assays. Tested for reliable amplification over a wide dynamic
range of template input and template type (genomic DNA, cDNA,
and plasmid DNA).
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Digital PCR Assays and Kits
Bio-Rad offers a comprehensive portfolio of Digital PCR Assays
and Kits for numerous applications:
- Mutation Detection
- Copy Number Determination
- Genome Edit Detection
- Gene Expression
- Residual DNA Quantification
- Library Quantification
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PCR & Real-Time PCR Instruments
Traditional PCR Systems
Thermal cyclers are essential laboratory equipment for
researchers who run polymerase chain reaction (PCR) for
sequencing, cloning, genotyping, mutagenesis, and many other
applications. Common features of Bio-Rad's thermal cyclers
include Peltier effect technology for precise temperature
control, and thermal gradient to easily optimize PCR assays in a
single run.
Instruments include the PTC Tempo Thermal Cycler, the next
generation of conventional PCR thermal cyclers from Bio-Rad.
Engineered to maintain thermal performance that delivers
accurate results, the PTC Tempo Thermal Cycler offers the
flexibility to scale with your laboratory. The new
design features a refreshed, intuitive user interface and
flexible connectivity options for streamlining protocol
management. A motorized lid enables automation, taking your
PCR to the next level with modern instrument control.
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qPCR Systems
Bio-Rad’s real-time PCR amplification systems combine thermal
cyclers with optical reaction modules for singleplex and
multiplex detection of fluorophores. Bio-Rad’s real-time PCR
detection systems range from economical two-target detection to
sophisticated five-target detection systems. All Bio-Rad
real-time detection platforms offer reliable gradient-enabled
thermal cyclers for rapid assay optimization.
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CFX
Opus 96
Real-Time PCR System
|
CFX
Opus 384
Real-Time PCR System
|
CFX
Duet
Real-Time PCR System
|
CFX
Opus Deepwell
Real-Time PCR System
|
| Features |
Powerful 5-channel multiplexing with our best thermal
uniformity and modernized data management options.
|
Our high-throughput system with 4-channel
multiplexing, automation capabilities, and more.
|
Accessible qPCR for essential molecular testing.
|
Industry-leading technology for large-volume reactions
in real-time PCR applications.
|
| Wells |
96 |
384 |
96 |
96 |
| Multiplex channels |
5 plus FRET |
4 plus FRET |
2 plus
FRET |
5 plus FRET |
| Touch screen |
Color |
Color |
Computer-controlled
operation only |
Color |
| Reaction volume |
1–50 µl |
1–30 µl |
1–50 µl |
10–125 µl |
Amplification Software
CFX Maestro Software
CFX Maestro Software is designed to streamline data collection,
visualization, and analysis. Precision Melt Analysis software
enables high resolution melt for SNP Genotyping and detection of
small indels.
CFX Maestro Software streamlines and supports the entire
process of real-time PCR experiments, including reference gene
selection, complex experiment setup, statistical analysis, and
publication-quality data graphing.
Available Editions: PC Edition | Mac Edition | Chinese Edition | Russian Edition | Security Edition (see
below)
CFX Maestro Software, Security Edition
CFX Maestro Software Security Edition adds a set of data
security tools to support compliance with U.S. FDA 21 CFR Part
11 regulations, working with the security features of Windows
operating systems, and requires a key that must be attached to a
USB port to function.
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Precision Melt Analysis Software
Precision Melt Analysis Software imports and analyzes data
files generated from all CFX Real-Time PCR Detection Systems to
genotype samples based on the thermal denaturation properties of
double-stranded DNA.
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PCR & qPCR Reagents, Assays, and Oligos
Assays and Panels for Real-Time PCR
PrimePCR real-time PCR assays were designed in collaboration
with leading experts in real-time PCR research at Biogazellle.
Every PCR primer pair has been experimentally validated to
ensure optimal assay performance. We offer:
- Predesigned primer and probe assays – gene
expression assays for the human and mouse genomes
- Custom oligonucleotides – design your own
PCR primer or probe assay
- Customizable 96- and 384-well plates –
use the custom plate configurator to
conveniently design a PCR plate
- Predesigned pathway panels – select from
a wide variety of disease- and pathway-specific panels
- Controls and Reference Assays are available
to facilitate assay normalization and control
All real-time PCR assays and panels can be used with CFX
Real-Time PCR Detection Systems and CFX Maestro Data Analysis
Software, streamlining PCR data collection and analysis.
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Real-Time PCR Supermixes and Kits
Bio-Rad's PCR reagents and kits feature advanced enzyme
technology and buffer formulations for reliable amplifcation,
robust reverse transcription, and high-quality real-time PCR
data.
Use our interactive selection tool to find the right products
for your needs. Filter by application, technique, detection
chemistry, or product-specific features to easily compare
reagents.
Go to Reagent Selector
SsoAdvanced Universal SYBR® Green Supermix
- Achieve superior real-time PCR results under various
experimental conditions – for example, primer
concentrations, annealing temperatures, salt concentrations,
and/or cycling protocol (standard or fast)
- Increase your qPCR sensitivity and efficiency of
detection from compromised samples – the highly
processive Sso7d fusion polymerase has increased resistance to
various PCR inhibitors, enhancing sensitivity and overall
performance
- Shorten time to results while maintaining qPCR data
quality – the Sso7d fusion polymerase and optimized
buffer provide rapid polymerization kinetics and instant
polymerase activation
- Use any real-time PCR system – the
universal reference dye in this supermix enables ROX
normalization of qPCR data regardless of the ROX level
requirements of the qPCR system
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SsoAdvanced Universal Probes Supermix
- Carry out high-performance singleplex and duplex
reactions – the highly processive Sso7d fusion
polymerase and advanced formulation enable robust performance
in singleplex or duplex qPCR, allowing cost and time savings
- Achieve superior real-time PCR results under various
experimental conditions – for example, primer
concentrations, annealing temperatures, salt concentrations,
and/or cycling protocol (standard or fast)
- Increase your qPCR sensitivity and efficiency of
detection from compromised samples – the Sso7d
fusion polymerase has increased resistance to a wide variety
of PCR inhibitors, enhancing sensitivity and overall
performance
- Shorten time to results without compromising qPCR
data quality – the Sso7d fusion polymerase and
optimized buffer provide rapid polymerization kinetics and
instant polymerase activation
- Use any real-time PCR system – the
universal reference dye in this supermix enables ROX
normalization of quantitative PCR (qPCR) data regardless of
the ROX level requirements of the qPCR system
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SsoFast EvaGreen Supermix
- Fast qPCR results and high performance —
instant activation and rapid polymerization kinetics generate
exceptional qPCR results in less than 30 min
- Minimal PCR inhibition — increased
resistance to PCR inhibitors ensures maximum efficiency,
sensitivity, and reproducibility
- Single-copy detection — provides high
sensitivity with amplification and detection from a single
copy of a target gene
- Robust discrimination and reproducibility —
achieves efficient discrimination and reliable quantification
from 1.33-fold serial dilutions of template
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iTaq Universal SYBR® Green Supermix
- Formulation developed for optimal results on any real-time
PCR instrument
- Advanced 2x ready-to-use supermix, formulated to deliver
robust qPCR results with superior sensitivity, efficiency, and
specificity
- Optimized buffer allows consistent results using both
standard and fast cycling protocols
- Antibody-mediated iTaq DNA polymerase enables fast
activation and superior specificity in qPCR
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iTaq Universal Probes Supermix
- Formulation developed for optimal results on any qPCR
instrument
- Optimized buffer allows consistent results for simplex and
duplex reactions using both standard and fast cycling
protocols
- Advanced 2x ready-to-use supermix, formulated to deliver
robust qPCR results with superior sensitivity, efficiency, and
specificity
- Antibody-mediated iTaq DNA polymerase enables fast
activation and superior specificity in qPCR
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iQ Multiplex Powermix
- Robust supermix formulated for sensitive and efficient
multiplex qPCR
- Reliable quantification of up to 4 targets (expression
levels can vary up to 106-fold between target
genes) or up to 5 targets
- Linearity over 6 orders of magnitude of input cDNA and 4
orders of magnitude of input genomic DNA
- Suitable for a wide variety of applications, including gene
expression analysis, SNP genotyping, SNP analysis, GMO
detection, and viral load detection
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Thermostable and High-Fidelity DNA Polymerases
iProof High-Fidelity DNA Polymerase
- Comprises a unique Pyrococcus-like proofreading
enzyme fused to a dsDNA binding protein, Sso7d
- High fidelity – iProof DNA polymerase is 52-fold more
accurate than Taq DNA polymerase
- Speed – high processivity dramatically reduces extension
(15–30 sec/kb) and overall reaction times
- Successful amplification of long products with higher yields
– fragments up to 37 kb are amplified in less time and with
less enzyme (0.25–1 unit/reaction)
- Comes in three convenient formats: a stand-alone enzyme, an
easy-to-use master mix, and a PCR kit complete with controls
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iTaq DNA Polymerase
- Antibody-mediated hot-start DNA polymerase is suitable for
both PCR and real-time PCR applications
- Needs only a short 3 minute activation step at 95°C
- iTaq DNA polymerase is highly specific, sensitive, and easy
to use
- Provides improved product yields compared to
nonantibody-based DNA polymerases
- Convenient reaction assembly at room temperature
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Other PCR Reagents
Precision Melt Supermix
- Optimized formulation containing EvaGreen dye delivers
robust PCR and high resolution melt (HRM) performance
- Sensitive and effective discrimination of all 4 SNP classes
across a broad range of amplicons
- Accurate detection of CpG methylation status for epigenetic
studies
- Exceptional room temperature stability for high-throughput
HRM studies
- Reliable performance on any HRM-capable thermal cycler
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dNTP Mix
- Formulated for optimal performance in real-time and standard
PCR applications
- Robust dNTP solution withstands multiple rounds of
freeze-thawing and temperature cycling
- Allows consistency and higher efficiency
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Reverse Transcription Reagents for RT-PCR
iScript
reverse transcription products are formulated for
efficient and sensitive reverse transcription over a wide linear
dynamic range of input RNA for real-time qPCR.
Reverse Transcription (for 2-step RT-qPCR)
iScript Advanced cDNA Synthesis Kit for RT-qPCR
- Increased qPCR data throughput and cost-effectiveness from a
single 20 μl reverse transcription (RT) reaction
- Superior sensitivity and broad linear dynamic range for RT
(7.5 μg–100 fg)
- 2-tube kit (5x iScript reaction mix and iScript reverse
transcriptase) for ease of use and reduced reaction setup time
- Optimized blend of oligo(dT) and random primers ensures
complete and unbiased RNA sequence representation
- RNase H+ MMLV reverse transcriptase (preblended with RNase
inhibitor) delivers high sensitivity for real-time RT-qPCR and
eliminates additional RNase H+ step
- Potent blend of RNase A inhibitor protects RNA during setup
and RT
- Short 35 minute protocol allows fast qPCR data generation
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iScript Reverse Transcription Supermix for RT-qPCR
- 1-tube format for simple and fast setup and reduced
pipetting variability
- Liquid format at –20°C offers superior stability and
eliminates freeze/thaw cycle
- Superior sensitivity and broad linear dynamic range for RT
(1 μg–100 fg)
- Optimized blend of oligo(dT) and random primers ensures
complete and unbiased RNA sequence representation
- RNase H+ MMLV reverse transcriptase (preblended with RNase
inhibitor) delivers high sensitivity for real-time RT-qPCR and
eliminates additional RNase H+ step
- Potent blend of RNase A inhibitor protects RNA during setup
and RT
- Short 40 minute protocol allows fast qPCR data generation
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iScript cDNA Synthesis Kit
- 2-tube kit (5x iScript reaction mix and iScript reverse
transcriptase) increases reproducibility and reduces reaction
setup time
- Optimized blend of oligo(dT) and random primers ensures
complete and unbiased RNA sequence representation
- RNase H+MMLV reverse transcriptase (preblended with RNase
inhibitor) delivers high sensitivity for real-time RT-PCR
- Dynamic range of 1 μg to 100 fg total RNA input (target
dependent)
- Short 40 minute protocol allows fast qPCR data generation
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iScript Select cDNA Synthesis Kit
- Separate tubes of oligo(dT) and random primers allows
selection of priming strategy for reverse transcription
- RNase H+MMLV reverse transcriptase (preblended with RNase
inhibitor) delivers high sensitivity for real-time RT-PCR
- Quality control provides reliable synthesis of long cDNA
greater than 6 kb in length
- A separate tube of enhancer solution provides maximum
sensitivity for reverse transcription with gene-specific
primers
- Dynamic range of 1 μg to 100 fg total RNA input (target
dependent)
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One-Step RT-PCR Kits
iTaq Universal SYBR® Green One-Step Kit
- Increase sensitivity, specificity, and efficiency –
advanced formulation enables robust performance and
increased resistance to a wide variety of sample types and
target sequences
- Use any real-time PCR system – the
universal reference dye in this reaction mix enables ROX
normalization of qPCR data regardless of the ROX level
requirements of the qPCR system
- Achieve superior real-time PCR results under any
condition – robust formulation delivers consistent
performance in fast cycling across a broad range of reaction
conditions, primer concentrations, and temperature ranges
- Obtain better results with PrimePCR assays – real-time
PCR assays are expertly designed and wet-lab validated to
ensure optimal assay performance
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iTaq Universal Probes One-Step Kit
- Increase sensitivity, specificity, and efficiency –
advanced formulation enables robust performance and
increased resistance to a wide variety of sample types and
target sequences
- Obtain superior results with multiplex reactions – enhanced
chemistry enables up to 3 target amplifications at the same
time, resulting in higher data precision with fewer pipet
steps and reduced sample usage
- Carry out high-throughput real-time PCR screening
and validation – simplified workflow and reduced
cycling times enable screening and validation of a great
number of samples and targets in a short period of time
- Use any real-time PCR system – the
universal reference dye in this reaction mix enables ROX
normalization of qPCR data regardless of the ROX level
requirements of the qPCR system
- Achieve superior real-time PCR results under any
conditions – robust formulation delivers
consistent performance in fast cycling across a broad range of
reaction conditions, primer concentrations, and temperature
ranges
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RT-PCR Directly from Cells
SingleShot Cell Lysis RT-qPCR Kits
- Cell lysate from 10 to 100,000 cells ready in 20 minutes
- Complete removal of genomic DNA while preserving RNA
integrity
- Optimal accuracy and linear dynamic range of qPCR data
across genes
- Results comparable to using purified RNA, including dynamic
range
- SingleShot RNA Control ensures optimal cell and lysate
inputs
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PCR Plastic Consumables
Our plastic consumables are precisely manufactured for optimal
fit and cycling performance and are certified to be free of
DNase, RNase, and human genomic DNA. Using high-quality PCR
plastics from Bio-Rad helps provide confidence in your PCR, qPCR
and next generation sequencing research.
Use the interactive PCR Plastics Selector to find the best
plates, tubes, seals, and accessories for your
PCR and qPCR experiments and determine which plates have the
best compatibility with your instrument.
Go to Plastics Selector
PCR Plates and Seals
Hard-Shell PCR Plates
Hard-Shell PCR Plates are engineered to withstand the stresses
of heat sealing, thermal cycling, and robotic handling,
featuring a patented two-component design for superior stability
and flatness.
- Superior well-to-well uniformity for reliable and
reproducible real-time qPCR results
- Automation Compatible - Warp resistance and superior
stability for robotic handling, high-speed centrifugation, and
storage
- Optional barcode with alphanumeric labels for easy well
identification in high-throughput settings
- Footprint and well spacing designed to match ANSI/SBS
standard dimensions
- Free of DNase, RNase, and human DNA
Hard-Shell
96-Well PCR Plates
Hard-Shell
384-Well PCR Plates
Custom PCR Plates
Hard-Shell PCR plates customized to your specifications. We
provide a well-defined and straightforward process to address
your PCR plastic needs, with customization options inlcuding:
- Barcoding — choose placement and sequences,
1D or 2D barcodes
- Packaging — select various boxes, including
standard white boxes
- Color — pick plate and well color
combinations
- Branding — include company name and logo on
PCR plates
- Labeling — customize the information
included on product packaging
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MicroSeal PCR Plate Seals
Choose from a wide range of pressure, adhesive, and
heat-sealing films and foils for PCR plates and other
microplates including:
- Peelable, pierceable, or permanent seals
- Seals optimized for PCR, qPCR, NGS, and sample storage
- Automation-compatible seals and sealing accessories
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PCR Tubes, Caps, and Accessories
PCR Tubes, Caps, and Strips
Individual 0.2 ml and 0.5 ml PCR tubes, 0.2 ml tube strips, and
cap strips with domed or optical flat tops
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PCR Plastics Accessories
A selection of handy tools, racks, and other accessories for
PCR plate and tube sealing, handling, and storage
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Sample Purification
Bio-Rad offers a complete line of kits and reagents for fast
and effective extraction and purification of RNA, DNA, and PCR
products.
RNA Isolation
Aurum Total RNA Mini Kit
Aurum Total RNA Kits are a family of isolation kits that
provide a high yield of intact RNA from a wide range of starting
materials, including cultured cells, bacteria, and yeast, as
well as plant and animal tissues.
- PCR-ready RNA in less than 60 min
- RNase-free reagents and plastic consumables ensure the
integrity of isolated RNA
- Kit includes DNase I provided for removal of gDNA
contamination
- Easy-to-use spin or vacuum protocol
Aurum Total RNA Isolation Kit variations:
PureZOL RNA Isolation Reagent
The ready-to-use PureZOL RNA isolation reagent is a versatile
and efficient means of isolating high yields of RNA from a
variety of sources, including cultured cell, animal and plant
tissue, yeast, virus, and bacteria samples.
- Single-solution format permits recovery of RNA from small
quantities of tissues or cells, making it ideally suited for
gene expression studies
- Efficient RNA purification from cultured cells, yeast,
viruses, and bacteria, as well as plant and animal tissues
- Efficient lysis of cells and tissues, deproteinates RNA, and
inactivates endogenous nucleases in a single step
- Scalable starting sample amount
- Convenient isolation of RNA, DNA, and protein from the same
sample
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iScript RT-qPCR Sample Preparation Reagent
- Enables RT-qPCR directly from cells without RNA purification
- Delivers efficient cell lysis, RNA stabilization, and
removal of genomic DNA for sensitive qPCR in 5 to 10 minutes
- Suitable for adherent and suspension animal cells
- Allows multiplex real-time detection of up to four targets
from as few as ten cells
- Ideal for rapid, high-throughput gene expression analysis
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DNA Isolation
InstaGene Matrix
InstaGene matrix, made with a specially formulated 6% w/v
Chelex resin, makes DNA sample preparation fast, easy, and
cost-effective, providing PCR-quality template DNA in less than
an hour.
- Ease of use — the small-particle-size slurry won't clog
pipet tips and is easily resuspended with the included stirbar
- Appropriate matrix concentration ready upon resuspension
- Affordability and cost-effectiveness — 100 samples from 1
bottle
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Quantum Prep Plasmid Kits
The consistently high yields achieved with Quantum Prep plasmid
kits are due to the high-affinity binding of DNA to the unique
Quantum Prep DNA binding matrix.
- High purity and yield of plasmid DNA
- Easy spin-column procedure to significantly reduce plasmid
DNA isolation time
- Stringent QC specifications ensure high-quality plasmid DNA
for immediate use in all downstream molecular biology
applications
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DNA Cleanup
Freeze ’N Squeeze DNA Gel Extraction Spin Columns
Freeze ’N Squeeze DNA gel extraction spin columns provide a
quick and effective means to purify double-stranded DNA
fragments from TAE- or TBE-buffered agarose gels.
- Requires less than 1 minute of hands-on time and less than
10 minutes for DNA cleanup
- No solutions to prepare
- DNA immediately available for subsequent PCR, subcloning,
ligations, and sequencing reactions
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