Life Technologies - Advanced gene expression services

Company Profile

Life Technologies biotechnology products and services, from instruments to everyday lab essentials, are created by scientists, for scientists. Customers worldwide use our innovative solutions every day in their pursuit to solve some of the most difficult biological challenges of the 21st century: translational research, personalized healthcare, stem cell therapies, food safety, animal health, and human identification. We are committed to moving science forward to help scientists improve life in meaningful ways.

Real-Time PCR
Applied Biosystems® pioneered the development of real-time PCR in 1995, enabling high-throughput, quantitative gene expression. Today, Applied Biosystems instruments and TaqMan® Assays offer industry leading performance for Gene Expression, miRNA profiling, SNP Genotyping, Copy Number Variation and Drug Metabolism Enzyme evaluation.

To learn more about our simple, affordable approach to real-time PCR and gene expression analysis, please select one of our featured products from the list below.

Real-Time PCR Instruments
StepOne™ Real-time PCR System
The StepOne™ Real-Time PCR System combines plug and play convenience, uncompromising performance and Fast high speed thermal cycling, that is tailor-made for both first-time users and real-time PCR experts.

StepOnePlus™ Real-Time PCR System
The Applied Biosystems
® StepOnePlus™ Real-Time PCR System, makes it simple and easy to get high-quality real-time PCR results.

7500 Real-Time PCR System
The Applied Biosystems
® 7500 Real-Time PCR System is a leading edge system with features to support labs requiring more capabilities from their real-time PCR platform.

7500 Fast Real-Time PCR System
The Applied Biosystems
® 7500 Fast Real-Time PCR System enables high speed thermal cycling in a 96-well format, reducing your run times to less than 40 minutes and accelerating your research by providing access to results more quickly than ever before.

7900HT Fast Real-Time PCR System
The Applied Biosystems
® 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading - and now also offers optional Fast real-time PCR capability.

The ViiA™ 7 Dx Real-Time PCR Instrument
The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to TaqMan® Array Micro Fludic Card, the ViiA™ 7 is available for in vitro diagnostics use.

QuantStudio™ real-time PCR  digital PCR system
Flexibility. Versatility. Speed. Precision. Everyone’s needs are unique and that’s why we have expanded the QuantStudio™ family of real-time PCR & digital PCR systems. Now you can pick the qPCR platform that best fits your research requirements—find your fit today.

Assays & Arrays
TaqMan® Gene Expression Assays
TaqMan® Gene Expression Assays provide over 1.3 million predesigned primer/probe sets covering 23 species, the most comprehensive set of quantitative gene expression assays available. Alternatively, custom assays enable you to study the expression of any gene or splice variant in any organism.

TaqMan® MicroRNA Assays
Innovative TaqMan® Assays for microRNA and other small RNAs, as well as longer noncoding RNA transcripts such as pri-miRNAs and long noncoding RNA. We offer products for noncoding RNA discovery, profiling, quantitation, validation, and functional analysis.

TaqMan® Assays for Genotyping & Genetic Variation Analysis
The precision of TaqMan® probe-based chemistry makes SNP genotyping and copy number variation studies more accurate than ever. Choose from 4.5 million human and mouse TaqMan® SNP Genotyping Assays or design your own Custom TaqMan® SNP Genotyping Assays. For analysis of SNPs in genes for drug metabolizing enzymes (DMEs), we offer 2,700 unique TaqMan® DME Assays that detect polymorphisms in 221 genes for DMEs and associated transporter targets.
For copy number variation studies, we offer over 1.6 million predesigned TaqMan® Copy Number Assays with human, genome-wide coverage, and mouse and marker/reporter assays. Custom and Custom Plus TaqMan® Copy Number Assay options are ideal when a predesigned assay for a target is not available.
TaqMan® Mutation Detection Assays
Competitive Allele-Specific TaqMan® PCR (castPCR™) technology detects and quantitates rare sequences in a background of common sequence by specifically blocking amplification of all but the rare-sequence variants. It has great potential for detection of rare mutations such as in circulating cancer cells.

TaqMan® Protein Assays
Revolutionary TaqMan® Protein Assays enable fast, easy identification, and relative quantification of protein markers from limited quantities of cultured cells. Choose from predesigned assays for human stem cell pluripotency markers and control proteins, or create your own assay from your biotinylated antibodies.

Custom TaqMan® Assay Manufacturing & Plating Service
If your research application requires special manufacturing modifications - such as a different dye or assay volume, or you would like to have your assays plated in a specific way, our custom services can help.

Primers & Probes Find oligonucleotides built to your specifications here. We offer options for virtually any application and delivery times to keep your research moving. Whether you need oligos for PCR, cloning, hybridization probes, or sequencing sample prep, Invitrogen, Applied Biosystems®, and TaqMan® custom oligo synthesis services offer the quality and convenience you’re looking for. Our predesigned oligos include trusted TaqMan® Assays for real-time PCR analysis of gene expression, genetic variation, and noncoding RNAs; Ambion® Silencer® Select siRNAs for RNA interference studies, and general use oligos and hybridization probes.

Invitrogen™ Custom DNA Oligos
DNA oligo products and services when you need them.
Custom oligos are offered with standard deoxynucleotides, modified bases and 5' and 3'-modified nucleotides.

Applied Biosystems® Custom TaqMan® Probes
Custom Applied Biosystems® oligonucleotides for use as primers and TaqMan® probes. Ideal for real-time PCR applications, sequencing, and fragment analysis sample prep.

Enzymes & Kits
TaqMan® Master Mixes
TaqMan® Master Mixes contain the buffers, dNTPs, internal reference dye, and - most importantly - hot-start DNA polymerase, that power real-time PCR. They were developed for use with our comprehensive selection of TaqMan® Assays to streamline reaction setup and therefore minimize variability among replicates, assays, and experiments.

View all TaqMan® Master Mixes
SYBR® Green Master Mixes
In SYBR® Green-based real-time PCR, amplification products are detected with fluorescent signal released by SYBR® Green dye, which fluoresces only when bound to double-stranded DNA. Our SYBR® Green Master mixes include buffers, dNTPs, internal reference dye, hot-start DNA Polymerase, and of course, SYBR® Green dye.

View all SYBR® Green Master Mixes

SuperScript® Reverse Transcription
RNA must be converted to cDNA in order to be amplified using PCR. We recommend the Invitrogen
SuperScript® VILO™ cDNA Synthesis Kit for reverse transcription prior to analyzing gene expression (mRNA), long noncoding RNA, and pri-miRNA using real-time PCR.
For analysis of microRNA and other small RNAs using TaqMan® Assays, we recommend the TaqMan® MicroRNA Reverse Transcription Kit.

One-Step Real-Time RT-PCR Kits
In one-step RT-PCR, reagents and enzymes for both reverse transcription (RT) and real-time PCR are added to RNA samples at the same time. Reactions are first incubated using conditions favorable for RT, then the resulting cDNA is immediately run in a real-time PCR reaction.
Sample-to-Answer Kits
With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required, saving time and reducing the chance of sample mixup or cross-contamination. Kits are available for gene expression, miRNA studies, and SNP genotyping.
High Resolution Melt (HRM) Master Mix
Mixed with your DNA sample and PCR primers, MeltDoctor™ HRM Master Mix provides the reagents needed to discriminate subtle differences in the target DNA sequence using post-PCR melting analysis.
TaqMan® OpenArray® Reagents
Specialized master mixes for use on the OpenArray® system.
MicroAmp® 96-Well Plates, 384-Well Plates, PCR Tubes, PCR Strip Tubes, and Adhesive Films
MicroAmp® plastics consumables offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. All of our plastics consumables are validated with Applied Biosystems® instruments for optimal fit and performance.

Useful links

qPCR Education

Contact Contact:

Thermo Fisher Scientific
Frankfurter Straße 129B
64293 Darmstadt
Phone:     49 6151 9670 0 
Fax:          49 6151 9670 5599 

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