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Life
Technologies - Advanced
gene expression services Company Profile Life
Technologies™ biotechnology products and
services, from instruments to
everyday lab essentials, are created by scientists, for scientists.
Customers worldwide use our innovative solutions every day in their
pursuit to solve some of the most difficult biological challenges of
the 21st century: translational research, personalized healthcare, stem
cell therapies, food safety, animal health, and human identification.
We are committed to moving science forward to help scientists improve
life in meaningful ways.
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| Real-Time
PCR |
Applied
Biosystems® pioneered the development
of real-time PCR in 1995, enabling
high-throughput, quantitative gene expression. Today, Applied
Biosystems instruments and TaqMan® Assays offer industry leading
performance for Gene Expression, miRNA profiling, SNP Genotyping, Copy
Number Variation and Drug Metabolism Enzyme evaluation. To learn more about our simple, affordable approach to real-time PCR and gene expression analysis, please select one of our featured products from the list below. |
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| Real-Time PCR Instruments |
StepOne™
Real-time PCR System The StepOne™ Real-Time PCR System combines plug and play convenience, uncompromising performance and Fast high speed thermal cycling, that is tailor-made for both first-time users and real-time PCR experts. StepOnePlus™ Real-Time PCR System The Applied Biosystems® StepOnePlus™ Real-Time PCR System, makes it simple and easy to get high-quality real-time PCR results. 7500 Real-Time PCR System The Applied Biosystems® 7500 Real-Time PCR System is a leading edge system with features to support labs requiring more capabilities from their real-time PCR platform. 7500 Fast Real-Time PCR System The Applied Biosystems® 7500 Fast Real-Time PCR System enables high speed thermal cycling in a 96-well format, reducing your run times to less than 40 minutes and accelerating your research by providing access to results more quickly than ever before. 7900HT Fast Real-Time PCR System The Applied Biosystems® 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading - and now also offers optional Fast real-time PCR capability. The ViiA™ 7 Dx Real-Time PCR Instrument The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to TaqMan® Array Micro Fludic Card, the ViiA™ 7 is available for in vitro diagnostics use. QuantStudio™ real-time PCR digital PCR system Flexibility. Versatility. Speed. Precision. Everyone’s needs are unique and that’s why we have expanded the QuantStudio™ family of real-time PCR & digital PCR systems. Now you can pick the qPCR platform that best fits your research requirements—find your fit today. |
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| Assays
& Arrays |
TaqMan® Gene Expression Assays TaqMan® Gene Expression Assays provide over 1.3 million predesigned primer/probe sets covering 23 species, the most comprehensive set of quantitative gene expression assays available. Alternatively, custom assays enable you to study the expression of any gene or splice variant in any organism. TaqMan® MicroRNA Assays Innovative TaqMan® Assays for microRNA and other small RNAs, as well as longer noncoding RNA transcripts such as pri-miRNAs and long noncoding RNA. We offer products for noncoding RNA discovery, profiling, quantitation, validation, and functional analysis. TaqMan® Assays for Genotyping & Genetic Variation Analysis The precision of TaqMan® probe-based chemistry makes SNP genotyping and copy number variation studies more accurate than ever. Choose from 4.5 million human and mouse TaqMan® SNP Genotyping Assays or design your own Custom TaqMan® SNP Genotyping Assays. For analysis of SNPs in genes for drug metabolizing enzymes (DMEs), we offer 2,700 unique TaqMan® DME Assays that detect polymorphisms in 221 genes for DMEs and associated transporter targets. For copy number variation studies, we offer over 1.6 million predesigned TaqMan® Copy Number Assays with human, genome-wide coverage, and mouse and marker/reporter assays. Custom and Custom Plus TaqMan® Copy Number Assay options are ideal when a predesigned assay for a target is not available. TaqMan® Mutation Detection Assays Competitive Allele-Specific TaqMan® PCR (castPCR™) technology detects and quantitates rare sequences in a background of common sequence by specifically blocking amplification of all but the rare-sequence variants. It has great potential for detection of rare mutations such as in circulating cancer cells. TaqMan® Protein Assays Revolutionary TaqMan® Protein Assays enable fast, easy identification, and relative quantification of protein markers from limited quantities of cultured cells. Choose from predesigned assays for human stem cell pluripotency markers and control proteins, or create your own assay from your biotinylated antibodies. Custom TaqMan® Assay Manufacturing & Plating Service If your research application requires special manufacturing modifications - such as a different dye or assay volume, or you would like to have your assays plated in a specific way, our custom services can help. |
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| Primers & Probes | Find
oligonucleotides built to your specifications here. We offer options
for virtually any application and delivery times to keep your research
moving. Whether you need oligos for PCR, cloning, hybridization probes,
or sequencing sample prep, Invitrogen™,
Applied Biosystems®, and
TaqMan® custom oligo synthesis services offer the quality and
convenience you’re looking for. Our predesigned oligos include trusted
TaqMan® Assays for real-time PCR analysis of gene expression,
genetic variation, and noncoding RNAs; Ambion® Silencer® Select
siRNAs for RNA interference studies, and general use oligos and
hybridization probes. Invitrogen™ Custom DNA Oligos DNA oligo products and services when you need them. Custom oligos are offered with standard deoxynucleotides, modified bases and 5' and 3'-modified nucleotides. Applied Biosystems® Custom TaqMan® Probes Custom Applied Biosystems® oligonucleotides for use as primers and TaqMan® probes. Ideal for real-time PCR applications, sequencing, and fragment analysis sample prep. |
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| Enzymes
& Kits |
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| SuperScript®
Reverse Transcription RNA must be converted to cDNA in order to be amplified using PCR. We recommend the Invitrogen™ SuperScript® VILO™ cDNA Synthesis Kit for reverse transcription prior to analyzing gene expression (mRNA), long noncoding RNA, and pri-miRNA using real-time PCR. For analysis of microRNA and other small RNAs using TaqMan® Assays, we recommend the TaqMan® MicroRNA Reverse Transcription Kit. One-Step Real-Time RT-PCR Kits In one-step RT-PCR, reagents and enzymes for both reverse transcription (RT) and real-time PCR are added to RNA samples at the same time. Reactions are first incubated using conditions favorable for RT, then the resulting cDNA is immediately run in a real-time PCR reaction.
With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required, saving time and reducing the chance of sample mixup or cross-contamination. Kits are available for gene expression, miRNA studies, and SNP genotyping.
Mixed with your DNA sample and PCR primers, MeltDoctor™ HRM Master Mix provides the reagents needed to discriminate subtle differences in the target DNA sequence using post-PCR melting analysis.
Specialized master mixes for use on the OpenArray® system. |
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| Assessories |
MicroAmp® 96-Well Plates, 384-Well
Plates, PCR Tubes, PCR Strip Tubes, and Adhesive Films MicroAmp® plastics consumables offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. All of our plastics consumables are validated with Applied Biosystems® instruments for optimal fit and performance. |
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Useful
links |
qPCR Education |
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| Contact | Contact: Thermo Fisher Scientific Frankfurter Straße 129B 64293 Darmstadt Germany Email: orders_germany@lifetech.com Phone: 49 6151 9670 0 Fax: 49 6151 9670 5599 |
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