Links to the WWW
Guide of Polymerase Chain Reaction Technique.
information please contact firstname.lastname@example.org
is an independent company dedicated to perform and to develop the
most sophisticated and accurate multichannel
validation systems, tests and techniques available on the market at this point
in time, all of which must be compliant with and related to reliable
PCR and RT-PCR
PCR and RPA &
others movies under
by Dolan DNA
Learning Center, Cold Spring Habor Laboratory
page for information and links on all aspects of the Polymerase Chain
polymerase chain reaction (PCR) is used in a wide variety of fields
including: molecular biology, environmental science, forensic science,
medical science, biotechnology, microbiology, the food industry,
diagnostic science, epidemiology, genetics, gene cloning, and many
This site provides scientists with a comprehensive directory for the
polymerase chain reaction (PCR) and related techniques.
laboratory was among the pioneers in quantitative real-time PCR and contributed by inventing the
light-up probes. We now strengthen our capability to handle
and take on commercial projects within the field of Q-PCR.
by OCTAVIAN HENEGARIU, Yale University
Quantitative Real-Time Polymerase Chain Reaction for
the Core Facility Using TaqMan and the Perkin-Elmer/Applied Biosystems
Division 7700 Sequence Detector
by Deborah S. Grove
Nucleic Acid Facility, Life Science Consortium, The
Pennsylvania State University, University Park, PA 16802
(1): real-time PCR Goes Prime Time
assays used for quantitative RT-PCR combine the best
attributes of both
relative and competitive (end-point) RT-PCR in that they
accurate, precise, capable of high throughput, and relatively easy
perform. To truly
appreciate the benefits of this technology, a review of PCR
multiplex PCR: guide and troubleshooting
Manual. Roche Molecular Biochemicals-Research
Biosystems Primer Design Tips
BioGuide - PCR
sequence tagged sites have been used as landmarks for construction of
various types of genomic maps. Using NCBI's "electronic PCR" program,
these sites can be detected in DNA sequences, potentially allowing
their map locations to be determined.
Reaction (PCR) Jump Station - An excellent web page for links and
Assistant PCR Troubleshooting Program.
DNA and PCR
Applied Biosystems Sequence Detection
Systems - NEWS
7700 FAQs & Tutorials
Notes & Multimedia
Products for LightCycler
of a Webcast on Real-Time PCR Applications (Bio.Com)
An excellent review on
Real-Time PCR (Bustin SA, 2000)
review on Product Differentiation by DNA Melting Point Curves (Ririe et
Handbooks on SYBR Green Detection Systems and RT-PCR
article on real-Time PCR platforms
Review of Molecular Beacons (by Robinson JK et al. )
technical article on Fluorescence Excitation and Emission on IDT Website
COMPLETE Alkami Quick GuideTM for
PCR is a
page manual with a file size of 296 KB.
It is formated
5.5" X 8.5" in order to make the Guide a
for use at your bench. The complete Guide will print
on 79 single-sided, letter sized, 8.5" X 11" sheets
you can cut and bind as desired. Click [HERE] to
your FREE copy of the Adobe Acrobat Reader.
PCR Primer design
Wittwer is Professor of Pathology at the University of Utah Medical
School and Director of
Flow Cytometry and
New Technology at Associated Regional and University Pathologists
Chief Technology Officer at Idaho Technology.
of Rapid Cycle Real Time PCR
presentation requires the RealPlayer 8 Basic.
on the LightCycler® Instrument
Calculation of the efficiency, "fit point method"
vs. "2nd derivate maximum method", noise-band, hybridisation
Rasmussen Ph.D. (Idaho Technology, Chief Operating Officer)