Symposium Sessions:
Main Topic: Molecular
diagnostics
Markers in diagnostic,
prognostic, and therapeutic,
markers on DNA, RNA, microRNA level, disease
markers, tissue specific markers, cancer markers, stem-cells markers,
differentiation
markers, methylation markers diagnostic
quantification methods, epigenetics, SNP analysis, high resolution melt
(HRM) applications, ...
Main Topic: Next
Generation Sequencing (NGS)
NGS
applications in Genomics, Epigenomics, RNA-Seq, microRNA analysis, CHIP
analysis; NGS data handling and analysis, ... ...
Main Topic: Transcriptional
Biomarkers
A
session about the discovery, identification and validation of
transcriptional biomarkers. In connection to this session a METHODS special issue "Transcriptional
Biomarkers"
(edited by Michael W. Pfaffl) is published in January 2013. At
the meeting all
participants will receive a print copy of
this special Methods
issue "Transcriptional
Biomarkers" METHODS 2013 59(1) pages 1-192
High
throughput
analysis
in
qPCR
High throughput and
gene
expression profiling platforms, 384 – 1536 well applications and more,
qPCR robotics; high throughput SNP application,
real-time
RT-PCR arrays (mRNA and microRNA), quantitative multiplexing …
Systems
biology
With
the main focus on agri-veterinarian systems biology
as a biology-based inter-disciplinary field that focuses on complex and
holistic interactions in biological systems.
Single-cells diagnostics
Single-cell sampling, circulating tumor cells
(CTS), pre-amplification
techniques, laser micro dissection, sub-cellular PCR,
micro-manipulation of
cell clusters, cellular micro injection, FACS spotting, single-cell
handling, …
MIQE & QM
strategies in qPCR
The MIQE guidelines: minimum
information for publication of quantitative real-time PCR experiments.
Following these guidelines will encourage better experimental practice,
allowing more reliable and unequivocal interpretation of qPCR results.
QM
strategies in real-time PCR to guarantee better and more valid
results...
non-coding RNAs -
microRNA, siRNA
and long non-coding RNAs
RNAi mechanism, extraction of non-coding
RNAs, RT-qPCR technologies to detect microRNA and long-non coding RNAs,
siRNA
applications
in combination with qRT-PCR, microRNA
targets and
microRNA precursors, new siRNA manipulation and microRNA
technologies, .....
Digital PCR
& Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and
clonally amplify
nucleic acids including DNA, cDNA, mRNA or microRNA. It allows a more
reliable
collection and sensitive measurement of nucleic acid amounts,
applications in copy
number variants, point mutations, and molecular diagnostics; …
Pre-analytical Steps
Pre-amplification, sampling technologies, DNA /
RNA purification, extraction
efficiency, DNA
/ mRNA / microRNA quality control, Reverse Transcription, RT quality
control,
external references, results from the SPIDIA study, ...
BioStatistics
& BioInformatics
qPCR & NGS data analysis
qPCR: Software applications, data
mining, calculation
of
relative expression, primer and probe design on mRNA and microRNA
level, real-time
PCR efficiency determination, mathematical modelling, multivariate
expression
profiling raw data analysis, statistics in
real-time PCR, data management, multi-way
expression profiling, multiple regression analysis, 3D data
visualization.......
NGS: Information technology in the era of NGS, data management,
mapping and alignment algorithms, data de novo assembly, NGS
application
on DNA, mRNA and microRNA level, comparison of NGS with conventional
high
throughput qPCR, and much more ... ...
Lunch
Seminars:
- qBASEplus - Data Analysis Lunch Seminar
- GenEx - Data Analysis Lunch Seminar
- Life Technologies - digital PCR Lunch Seminar
- Genomatix - NGS data analysis Lunch Seminar
Abstract
submission deadline:
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Deadline is 8th February 2013
Please
register
using
the
Internet based ConfTool registration and submission platform => https://www.conftool.com/qPCR-NGS-2013/
- Register as ConfTool User
=>
NEW USER => Create new
account
- Register as symposium PARTICIPANT
=>
for the
symposium
and/or workshops
- Submit
Contribution =>
submit
your TALK and/or POSTER abstract(s)
- Please
indicate in which Session
you want to present either a POSTER or give an TALK
Presentation via MS PowerPoint
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The
official conference and workshop language is ENGLISH.
No
simultaneous
translation
will
be provided.
All oral presentations, posters and abstracts must be presented in English
All
scientific
contributions
will be published in the qPCR
& NGS 2013 Symposium Proceedings - ISBN to
be announced The
symposium
proceedings will including
all
Abstracts,
Posters and Oral Presentations. Proceedings will be published as
FULL PDF PRESENTATIONS on the symposium web page, as in the previous
years. As
wanted
by the authors the PDF download of the
contributions is password protected and only accessible for Symposium
or Application Workshop participants. |
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Poster Layout:
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- Until
autumn 2013 the PDF download of the contributions is password
protected
and only accessible for Symposium and Workshop participants.
- The
abstract should be a one-page document based on unpublished material
and
written in good standard English. The text should clearly state
objectives,
methodology, results, and conclusion. Simple tables and graphs are
allowed.
- Poster will be
DIN A0 (portrait format
=> 120
cm/48
inch high x 90 cm/35 inch wide)
Presentation
of every communication at the Conference is subjected to formal
registration
of at least one co-author.
- All
scientific contributions will be published in the qPCR & NGS 2013 Symposium Proceedings
- ISBN to be announced
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