Main Topic: Molecular
diagnostics
Markers in diagnostic,
prognostic, and therapeutic,
markers on DNA, RNA, microRNA level, disease
markers, tissue specific markers, cancer markers, stem-cells markers,
differentiation
markers, methylation markers diagnostic
quantification methods, epigenetics, SNP analysis, high resolution melt
(HRM) applications, ...
Main Topic: Next
Generation Sequencing (NGS)
NGS
applications in Genomics, Epigenomics, RNA-Seq, microRNA analysis, CHIP
analysis; NGS data handling and analysis, ... ...
Main Topic: Transcriptional
Biomarkers
A
session about the discovery, identification and validation of
transcriptional biomarkers. In connection to this
session a METHODS special issue
"Transcriptional
Biomarkers"
(edited by Michael W. Pfaffl) is published in January 2013. At
the meeting all
participants will receive a print copy of
this special Methods
issue "Transcriptional
Biomarkers" METHODS 2013 59(1) pages 1-192
High
throughput
analysis
in
qPCR
High throughput and
gene
expression profiling platforms, 384 – 1536 well applications and more,
qPCR robotics; high throughput SNP application,
real-time
RT-PCR arrays (mRNA and microRNA), quantitative multiplexing …
Systems
biology
With
the main focus on agri-veterinarian systems biology
as a biology-based inter-disciplinary field that focuses on complex and
holistic interactions in biological systems.
Single-cells diagnostics
Single-cell sampling, circulating tumor cells
(CTS), pre-amplification
techniques, laser micro dissection, sub-cellular PCR,
micro-manipulation of
cell clusters, cellular micro injection, FACS spotting, single-cell
handling, …
MIQE & QM
strategies in qPCR
The MIQE guidelines: minimum
information for publication of quantitative real-time PCR experiments.
Following these guidelines will encourage better experimental practice,
allowing more reliable and unequivocal interpretation of qPCR results.
QM
strategies in real-time PCR to guarantee better and more valid
results...
non-coding RNAs -
microRNA, siRNA
and long non-coding RNAs
RNAi mechanism, extraction of non-coding
RNAs, RT-qPCR technologies to detect microRNA and long-non coding RNAs,
siRNA
applications
in combination with qRT-PCR, microRNA
targets and
microRNA precursors, new siRNA manipulation and microRNA
technologies, .....
Digital PCR
& Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and
clonally amplify
nucleic acids including DNA, cDNA, mRNA or microRNA. It allows a more
reliable
collection and sensitive measurement of nucleic acid amounts,
applications in copy
number variants, point mutations, and molecular diagnostics; …
Pre-analytical Steps
Pre-amplification, sampling technologies, DNA /
RNA purification, extraction
efficiency, DNA
/ mRNA / microRNA quality control, Reverse Transcription, RT quality
control,
external references, results from the SPIDIA study, ...
BioStatistics
& BioInformatics
qPCR & NGS data analysis
qPCR: Software applications, data
mining, calculation
of
relative expression, primer and probe design on mRNA and microRNA
level, real-time
PCR efficiency determination, mathematical modelling, multivariate
expression
profiling raw data analysis, statistics in
real-time PCR, data management, multi-way
expression profiling, multiple regression analysis, 3D data
visualization.......
NGS: Information technology in the era of NGS, data management,
mapping and alignment algorithms, data de novo assembly, NGS
application
on DNA, mRNA and microRNA level, comparison of NGS with conventional
high
throughput qPCR, and much more ... ...
Lunch Time Seminars:
- qBASEplus - Data
Analysis Seminar
- GenEx - Data
Analysis Seminar
- Life Technologies
- digital PCR Seminar
- Genomatix - NGS data analysis Seminar