Confirmed Speakers & preliminary Talk Titles



Main Topic:   Molecular diagnostics
Russ Higuchi
Ph.D., Cepheid Fellow R&D, Cepheid, Sunnyvale, CA, USA


Keynote presentation:
Near-Oncology Patient (and Near-Oncology Health Care Provider) Molecular Testing on the GeneXpert

Hendrik Dietz
Prof. of Biomolecular Nanotechnology, Physik Department & Walter Schottky Institute, Technische Universität München, Germany


Shape, sequence, function - synthetic biology with DNA

Steve Lefever
Center for Medical Genetics Ghent, Ghent University, Ghent, Belgium


Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR

Olfert Landt
CEO, TIB-Molbiol, Berlin, Germany


Going to the limits of Multiplex Real-time PCR

Lars Ullerich
R&D, GNA Biosolutions GmbH, Planegg / Martinsried, Germany


An integrated molecular assay with laser-heated nanoparticles, performed in 20 minutes

Mikael Kubista
Prof. of Biotechnology, BTU, Czech Academy of Sciences & TATAA Biocenter, Sweden


High throughput mRNA and protein expression profiling by qPCR

Ralf Mauritz
R&D,
Roche Diagnostics GmbH, Nonnenwald, Penzberg, Germany

The new LightCycler 96 System:  It is so easy to be a LabHero



Main Topic:   Next Generation Sequencing  (NGS)
Alexander Schramm
Senior Scientist, Universitätsklinikum Essen, Kinderklinik, Onkologisches Labor, Essen, Germany


RNA-Seq:  opportunities, limitations and applications in cancer research

Vladimir Benes
Head of GeneCore, EMBL, Heidelberg, Germany


Advances in NGS library preparartion
Bram De Wilde
Center for Medical Genetics Ghent, Ghent University
, Ghent, Belgium


NXTVAT:  cloud software for NGS data-analysis
Michael W. Pfaffl
Prof. of Molecular Physiology, Chair of Physiology, TUM Weihenstephan, Germany


Functional small-RNAs in blood and milk

Ralph Vogelsang
Pacific Biosciences, Willow Road, Menlo Park, CA, USA


Sequencing the “Unsequenceable”
Single-Molecule, Real-Time (SMRT™) DNA Sequencing: Technology Overview and Recent Applications

Götz Frommer
R & D, Agilent Technologies, Böblingen, Germany

New developments in NGS target enrichment

Richard Fekete
R & D, Life Technologies, Foster City, CA, USA

Advancements in Ion Torrent RNA Sequencing: Less AND More

Svilen Tzonev
Head of Computational Biology, Digital Biology Center, Bio-Rad Laboratories, CA, USA

Droplet Digital PCR and Next Gen Sequencing: Digital Biology in High Definition

Oldroyd, Nicola
R&D, Illumina, United Kingdom


The Potential for Next Generation Sequencing in Forensics

Erik Söderbäck
R&D, Qiagen, Hilden, Germany

Transforming NGS in Clinical Research and Healthcare - The New Scalable GeneReader Sample to Result Workflow

Eric van der Walt
PhD, Senior Scientist, Kapa Biosystems, Black River Business Park, Cape Town, South Africa


Engineered Enzymes and Optimized Workflows for Next Generation Sequencing




Main Topic:   Transcriptional Biomarkers
Stephen A Bustin
Professor of Allied Health and Medicine, Postgraduate Medical Institute, Faculty of Health, Social Care & Education, Anglia Ruskin University, UK


Expression analysis tissue culture cells grown in long-term (up to 1 year) organotypic culture

Dave Ruff
R&D, Life Technologies, Foster City, CA, USA


Ion AmpliSeq Technology:  A tool for biomarker discovery and beyond

Stefan Bauersachs
PhD, LMU, Munich, Germany


Transcriptional Profiling To Address Molecular Determinants Of Endometrial Receptivity – Lessons From Studies In Livestock Species

Michael Rhodes
R&D, Nanostring Technologies, UK

Single-Cell Digital Gene Expression On Up To 800 Unique Transcripts Using Optically-Barcoded Single-Nucleic Acid Counting: Comparison With Microfluidic qPCR And RNA-seq (Whole Transcriptome)



High throughput analysis &
Optimization in qPC
R

Jan Hellemans
Biogazelle, Technologiepark 3, B-9052 Zwijnaarde, Belgium


Unique benefits of qPCR demonstrated by the first qPCR transcriptome wide expression profiling

Patricia de Winter
PhD, University College London, London, UK


Rapid qPCR using a novel Taq mutant

Dave Schuster
R & D, Quanta BioSciences, Perry Parkway, Gaithersburg, MD, USA

Overcoming PCR inhibition:  Next Generation qPCR and RT-qPCR ToughMix® Reagents for High Sensitivity Quantification and Accurate Genotyping from Crude Samples

Rainer Schubbert
Head of Operations Applied Genetics, Eurofins Medigenomix, Ebersberg, Germany

Twisted Intercalating Nucleic Acid (TINA) ­ a novel groupof molecules with improved performance
in PCR and qPCR applications

Gothami Padmabandu
R&D, Illumina, San Diego, USA

A Nû Solution for Real-Time Quantitative PCR

Ossian Saris
R&D, Thermo Fisher Scientific, Finland


Factors influencing the transfer of multiplex assays between qPCR instruments
Celeste Glazer
R&D, Labcyte, Sunnyvale, CA, USA

Automated PCR Setup for High Throughput Analysis Enabled by the Labcyte Echo® 525 Liquid Handler and Access™ Workstation

Stefaan Derevaux
WaferGen Biosystems Europe, L-1210 Luxembourg, Luxembourg

The WaferGen’s SmartChip System: “Cycling in the world of high- throughput qPCR and target enrichment“



Systems Biology
Massimo Bionaz
Assistant Professor, Dairy Management, Animal and Rangeland Sciences, Oregon State University, Corvallis, USA


Keynote presentation:
Systems Physiology in Cattle:  Transcriptome Dynamics and Beyond


Robert Loewe
CEO, GeneWake GmbH, Neuried,Germany

A system biology approach utilizing in situ PCR, qPCR and NGS - a Toolbox to decipher cancer



Single-Cell Diagnostics
Ken Livak
Fluidigm, South San Francisco, CA, USA
Keynote presentation:
A Microfluidic Device that Isolates Single Cells then Processes RNA for qPCR or Sequencing

Anders Ståhlberg
Senior Scientist, Sahlgrenska Cancer Center, University of Gothenburg, Sweden


Cell Transitions at Single-Cell Level
Christian Korfhage
R&D, Qiagen, Hilden, Germany

Genome Analysis Of Individual Cells

Marshall Levesque
Bioengineering at University of Pennsylvania, USA


Visualizing gene expression at the single cell, single chromosome, single RNA, and single base level



MIQE & QM strategies in qPCR
Stephen A Bustin
Professor of Allied Health and Medicine, Postgraduate Medical Institute, Faculty of Health, Social Care & Education, Anglia Ruskin University, UK

Keynote presentation:
MIQE - its impact four years after publication

Matjaž Hren
BioSistemika Ltd, Tehnološki park 24, 1000 Ljubljana, Slovenia


Management and Automation of qPCR Diagnostic Workflows

Afif Michel Abdel Nour
Associate Professor, Special Infectious Agents Unit – Biosafety Level 3, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia



Applying The MIQE guidelines to clinical and pre-clinical trials
Gregory Shipley
Shipley Consulting LLC, Houston, Texas, USA
former Director of the Quantitative Genomics Core Laboratory at UTHSC-Houston



Applying the MIQE Guidelines to Screens Utilizing qPCR Focused Arrays

Jan M Ruijter
Department of Anatomy, Embryology and Physiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands


The use and usefulness of amplification curve analysis in quantitative PCR

Kristina Lind
Senior Sceintist, TATAA Biocenter Sweden, Göteborg, Sweden


Quality control in Quantitative PCR


Andreas Untergasser
University Heidelberg, ZMBH, Michelstadt, Germany



Four Years of RDML qPCR Data Format – Achievements and Opportunities


non-coding RNAs - microRNA, siRNA and long non-coding RNAs
Jo Vandesompele
Prof. at the Center for Medical Genetics Ghent, Ghent University, Ghent, Belgium

Keynote lecture:
Evaluation of quantitative microRNA gene expression platforms in the microRNA Quality Control (miRQC) study

Mirco Castoldi
Clinic of Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University, Duesseldorf, Germany


High throughput analysis of Asthma-associated circulating microRNAs
Joerg Hackermueller
Young Investigators Group Bioinformatics and Transcriptomics , Helmholtz Centre for Environmental Research - UFZ
RNomics group, Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany


Non-coding RNAs in tumor and inflammatory diseases
Pieter Mestdagh
Center for Medical Genetics Ghent, Ghent University, Ghent, Belgium


High-throughput lncRNA expression profiling identifies candidate cancer lncRNAs
Angel Rubio
Group of Bioinformatics, CEIT and TECNUN, University of Navarra, San Sebastian, Spain


Integration of disparate sources of information to predict miRNA-mRNA interactions

Swanhild Meyer
Physiology Weihenstephan, Technische Universität München, Freising, Germany



How to narrow down the complexity of possible miRNA and mRNA interactions in cellular differentiation?

Ditte Andreasen
Scientific Manager - qPCR Development, Exiqon, Denmark

Generating Robust Results From qPCR Analysis Of MicroRNAs In Biofluids

Jonathan Michael Shaffer,
R&D, Qiagen, United States of America

An Optimized miRNA Profiling System for Limiting Samples



Digital PCR  &  Nano-fluidics
Jim Huggett
Science Leader Nucleic Acid Metrology, LGC

Keynote presentation:
An introduction to digital PCR
Ariane De Ganck
Biogazelle, Technologiepark 3, B-9052 Zwijnaarde, Belgium


Use of digital PCR for improved copy number quantification

David Keys
R & D, Life Technologies, Foster City, CA, USA

Performance of a next generation fixed microwell platform for digital PCR

Jennifer Meadows
Dept Medical Biochemistry & Microbiology (IMBIM), Uppsala University, Sweden


Enhanced Resolution of Copy Number Variants in Domestic Animal Species

Adam Corner
Field Application Scientist - Europe, RainDance Technologies, Lexington, MA, USA

RainDrop Digital PCR: Single Molecule Counting With A Droplet Digital PCR Platform



BioStatistics & BioInformatics
qPCR & NGS data analysis

John Quackenbush
Professor of Computational Biology and Bioinformatics, Department of Biostatistics
Dana-Farber Cancer Institute, Boston, Massachusetts, USA

Keynote presentation:
The road to personalized medicine is paved with data and information

Rolf Knobel
R&D, Roche Diagnostics International, Rotkreuz, Switzerland

A Kinetic PCR model function and its application on the Light Cycler 96



Lunch Time Seminars

Barbara D'haene
Biogazelle, Technologiepark 3, B-9052 Zwijnaarde, Belgium

qBASEplus - data analysis lunch time seminar

qbasePLUS speeds up the analysis of your qPCR data and improves the accuracy of your results



Mikael Kubista & Amin Forootan
MultiD & TATAA Biocenter Sweden, Göteborg, Sweden

GenEx - data analysis lunch time seminar

GenEx ­ - the ultimate tool for qPCR data analysis



David Keys
R & D, Life Technologies, Foster City, CA, USA
digital PCR lunch time seminar

Digital PCR Workshop with the QuantStudio 3D

Christian Zinser
Head of Consulting, Genomatix Software GmbH, Munich, Germany

NGS data analysis lunch time seminar

Start making sense - NGS data analysis with Genomatix




Please direct your enquiry to our scientific organisation team, headed by Michael W. Pfaffl  qPCR-NGS-2013@wzw.tum.de
©  2012 - 2013