CALL for PAPERS

---

Talk and Poster Sessions to be presented at the event:

All scientific contributions will be published in the qPCR & NGS 2015 Symposium Proceedings  ( ISBN 978-3-00-048805-4 )

Main topic:   Advanced Molecular Diagnostics
This session is focusing on the application of highly sophisticated methods, application and algorithms to discover, detect, and validate molecular diagnostic markers. A special focus is lying on the integrative analysis of multi level biomarkers, e.g. microRNA – mRNA, integrative analysis of genomic, proteomic, metabolomic and phenotypic markers.

Main topic:   Biomarker Discovery
A session about the discovery, identification and validation of molecular biomarkers:  diagnostic, prognostic or therapeutic markers on DNA, RNA, microRNA or small RNA, metabolome and proteome level (e.g. disease markers, cancer or stem-cells markers, tissue specific markers, differentiation markers, methylation markers). New assay systems will be presented like high throughput proteomics (MS based proteome map), PLA (Proximity Ligation Assay) or PEA (Proximity Elongation Assay).

Main topic:   Next Generation Sequencing (NGS)
NGS applications offer new holistic analysis of any kind of nucleic acid, to investigate the Genome, Exome, Epigenome, Transcriptome and Splicome (total RNA, microRNA, small RNA Seq, long ncRNA), CHIP purified nucleic acids, etc.
Various NGS sub-sessions will be presented:
•    NGS overview talks - information technology in the era of NGS
•    Pre NGS - sample prep & setup & library generation
•    NGS – new sequencing technologies (e.g. single molecule and pore sequencing)
•    NGS – data analysis (data management, mapping, alignment algorithms, data de novo assembly )
•    NGS – diagnostic applications

MicroGenomics & Single-cells diagnostics
Focus is on micro-genomics, the application of molecular methods to detect biomarkers in a minimal amount of tissue or matrix. New applications in single-cell isolation, separation or characterisation technologies, laser micro dissection, isolating circulating tumour cells (CTC), pre-amplification techniques, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection, , single-cell handling, FACS sorting and spotting.

Circulating Nucleic Acids
A new class of biomarkers are circulating nucleic acids (DNA, RNA, small RNAs, and long ncRNAs) which are free floating (?), bound to proteins or covered in micro-vesicles. One focus will be on exosome or micro-vesicles isolation, purification for bio-liquids, amount and size quantification, and characterisation techniques, including intra-cellular and membrane bound protein or lipid markers.

Molecular Diagnostics in Agriculture, Veterinary Medicine, Food & Environmental Science
This session is dedicated to molecular diagnostics in Life Science, with focus on Agriculture, Veterinary Medicine, and Environmental Science (which is the major research focus here at the TUM campus in Weihenstephan). All applications of highly sophisticated quantification methods, field application or algorithms to research the wide field of agro-veterinarian life science are very welcome.

MIQE & QM & Standardisation strategies in molecular diagnostics
This session is focusing on standardisation strategies and quality management in molecular diagnostics. The goal is to guarantee better and more valid results. Of special interest in the context of qPCR are the MIQE guidelines (minimum information for publication of quantitative real-time PCR experiments). Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.

Digital PCR  &  Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and clonally amplify nucleic acids including DNA, cDNA, mRNA or microRNA. It allows a more reliable collection and highly sensitive measurement of nucleic acid amounts, applications in copy number variants, point mutations in molecular diagnostics.

Non-coding RNAs -- microRNA, small RNAs, long non-coding RNAs
This session is dedicated to the family of non-coding RNA and its RNAi mechanism and applications:  extraction of non-coding RNAs, RT-qPCR technologies to detect microRNA, long-non coding RNAs and new classes of small RNAs, siRNA knock down applications, microRNA targets and microRNA precursors, new siRNA manipulation, etc.

qPCR Data Analysis -- BioStatistics & BioInformatics
The data analysis in RT-qPCR expression profiling experiments is still challenging and time consuming. From the literature it is well known, that a lot of error is introduced by wrong experimental study design, reference gene normalisation or qPCR data analysis. This session is focusing on useful algorithms and software applications for data mining, calculation of relative expression, primer and probe design for any kind of nucleic acid (mRNA, microRNA, piRNA), real-time PCR efficiency determination, mathematical modelling, multivariate expression profiling raw data analysis, statistics in real-time PCR, data management, multi-way expression profiling, multiple regression analysis, 3D data visualization, and much more!

---

Important dates:

Abstract submission deadline:	Abstract submission deadline is 6th Feb 2015 Please register using the Internet based ConfTool registration & submission platform => registration.qPCR-NGS-2015.net Register as ConfTool User  =>  NEW USER  =>  Create new account Register as symposium PARTICIPANT  =>  for the symposium and/or workshops Submit Contribution  =>  submit your TALK and/or POSTER  abstract(s) Please indicate in which Session you want to present either a POSTER or give an TALK Presentation via MS PowerPoint The abstract should be a one-page document based on unpublished material and written in good standard English. The text should clearly state objectives, methodology, results, and conclusion. Simple tables and graphs are allowed. All scientific contributions will be published in the qPCR & NGS 2015 Symposium Proceedings  (ISBN 978-3-00-048805-4)
Symposium:	The official conference and workshop language is ENGLISH No simultaneous translation will be provided. All oral presentations, posters and abstracts must be presented in English All scientific contributions will be published in the qPCR & NGS 2015 Symposium Proceedings  -  ISBN 978-3-00-048805-4 The symposium proceedings willinclud all Abstracts, Posters and Oral Presentations. Proceedings will be published as FULL PDF PRESENTATIONS on the symposium web page. Please note -- All talks will be recorded! In Summer 2015 the recorded talks will be published on www.eConferences.de  Until autumn 2015 the recorded tallks are password protected and only accessible for Symposium and Workshop participants.
Poster Layout:	The official conference and workshop language is ENGLISH All oral presentations, posters and abstracts must be presented in English Until autumn 2015 the PDF download of the poster contributions are password protected and only accessible for Symposium and Workshop participants. Poster will be DIN A0   (portrait format  =>  120 cm/48 inch high x 90 cm/35 inch wide) Presentation of every communication at the Conference is subjected to formal registration of at least one co-author.
Symposium Talks: (each talk 30 min)	Monday             23rd of March        10:00 a.m.  to  6:00 p.m. Tuesday            24th of March         8:30 a.m.  to   6:00 p.m. Wednesday      25th of March         8:30 a.m.  to   5:00 p.m.