
CALL
for PAPERS
Talk
and Poster Sessions to be presented at the
event:
All
scientific
contributions
will be published in the qPCR
& NGS 2015 Symposium Proceedings ( ISBN
978-3-00-048805-4 )
Main
topic: Advanced Molecular Diagnostics
This session is focusing on the application of highly
sophisticated methods, application and algorithms to discover, detect,
and validate molecular diagnostic markers. A special focus is lying on
the integrative analysis of multi level biomarkers, e.g. microRNA –
mRNA, integrative analysis of genomic, proteomic, metabolomic and
phenotypic markers.
Main
topic: Biomarker Discovery
A session about the discovery, identification and
validation of
molecular biomarkers: diagnostic, prognostic or therapeutic
markers on
DNA, RNA, microRNA or small RNA, metabolome and proteome level (e.g.
disease markers, cancer or stem-cells markers, tissue specific markers,
differentiation markers, methylation markers). New assay systems will
be presented like high throughput proteomics (MS based proteome map),
PLA (Proximity Ligation Assay) or PEA (Proximity Elongation Assay).
Main
topic: Next Generation Sequencing (NGS)
NGS applications offer new holistic analysis of any
kind of
nucleic acid, to investigate the Genome, Exome, Epigenome,
Transcriptome and Splicome (total RNA, microRNA, small RNA Seq, long
ncRNA), CHIP purified nucleic acids, etc.
Various NGS sub-sessions will be presented:
• NGS overview
talks - information technology in the era of NGS
• Pre NGS -
sample prep & setup & library generation
• NGS – new
sequencing technologies (e.g. single molecule and pore sequencing)
• NGS – data
analysis (data management, mapping, alignment algorithms, data de novo
assembly )
• NGS –
diagnostic applications
MicroGenomics
& Single-cells diagnostics
Focus is on micro-genomics, the application of molecular
methods
to detect biomarkers in a minimal amount of tissue or matrix. New
applications in single-cell isolation, separation or characterisation
technologies, laser micro dissection, isolating circulating tumour
cells (CTC), pre-amplification techniques, sub-cellular PCR,
micro-manipulation of cell clusters, cellular micro injection, ,
single-cell handling, FACS sorting and spotting.
Circulating
Nucleic Acids
A new class of biomarkers are circulating nucleic acids
(DNA,
RNA, small RNAs, and long ncRNAs) which are free floating (?), bound to
proteins or covered in micro-vesicles. One focus will be on exosome or
micro-vesicles isolation, purification for bio-liquids, amount and size
quantification, and characterisation techniques, including
intra-cellular and membrane bound protein or lipid markers.
Molecular
Diagnostics in Agriculture, Veterinary Medicine, Food &
Environmental
Science
This session is dedicated to molecular diagnostics in
Life
Science, with focus on Agriculture, Veterinary Medicine, and
Environmental Science (which is the major research focus here at the
TUM campus in Weihenstephan). All applications of highly sophisticated
quantification methods, field application or algorithms to research the
wide field of agro-veterinarian life science are very welcome.
MIQE
& QM & Standardisation strategies in molecular diagnostics
This session is focusing on standardisation strategies
and
quality management in molecular diagnostics. The goal is to guarantee
better and more valid results. Of special interest in the context of
qPCR are the MIQE guidelines (minimum information for publication of
quantitative real-time PCR experiments). Following these guidelines
will encourage better experimental practice, allowing more reliable and
unequivocal interpretation of qPCR results.
Digital
PCR & Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and
clonally
amplify nucleic acids including DNA, cDNA, mRNA or microRNA. It allows
a more reliable collection and highly sensitive measurement of nucleic
acid amounts, applications in copy number variants, point mutations in
molecular diagnostics.
Non-coding
RNAs -- microRNA, small RNAs, long non-coding RNAs
This session is dedicated to the family of non-coding
RNA and
its RNAi mechanism and applications: extraction of non-coding
RNAs,
RT-qPCR technologies to detect microRNA, long-non coding RNAs and new
classes of small RNAs, siRNA knock down applications, microRNA targets
and microRNA precursors, new siRNA manipulation, etc.
qPCR
Data Analysis -- BioStatistics & BioInformatics
The data analysis in RT-qPCR expression profiling
experiments is
still challenging and time consuming. From the literature it is well
known, that a lot of error is introduced by wrong experimental study
design, reference gene normalisation or qPCR data analysis. This
session is focusing on useful algorithms and software applications for
data mining, calculation of relative expression, primer and probe
design for any kind of nucleic acid (mRNA, microRNA, piRNA), real-time
PCR efficiency determination, mathematical modelling, multivariate
expression profiling raw data analysis, statistics in real-time PCR,
data management, multi-way expression profiling, multiple regression
analysis, 3D data visualization, and much more!
Important dates:
Abstract
submission deadline:
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Abstract submission deadline
is 6th Feb 2015
Please
register
using
the
Internet based ConfTool registration & submission platform => registration.qPCR-NGS-2015.net
- Register as ConfTool User
=>
NEW USER => Create new
account
- Register as symposium PARTICIPANT
=>
for the
symposium
and/or workshops
- Submit
Contribution =>
submit
your TALK and/or POSTER abstract(s)
- Please
indicate in which Session
you want to present either a POSTER or give an TALK
Presentation via MS PowerPoint
- The
abstract should be a one-page document based on unpublished material
and
written in good standard English.
- The text should clearly state
objectives,
methodology, results, and conclusion. Simple tables and graphs are
allowed.
- All
scientific contributions will be published in the qPCR & NGS 2015
Symposium Proceedings
(ISBN 978-3-00-048805-4)
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Symposium:
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- The
official conference and workshop language is ENGLISH
- No
simultaneous
translation
will
be provided.
- All oral presentations, posters and
abstracts must be presented in English
- All
scientific
contributions
will be published in the qPCR
& NGS 2015 Symposium Proceedings - ISBN
978-3-00-048805-4
- The
symposium
proceedings willinclud
all
Abstracts,
Posters and Oral Presentations. Proceedings will be published as
FULL PDF PRESENTATIONS on the symposium web page.
- Please note -- All
talks will be recorded!
In Summer 2015 the recorded
talks
will be published on www.eConferences.de
Until
autumn 2015 the recorded tallks are password
protected
and only accessible for Symposium and Workshop participants.
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Poster Layout:
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- The
official conference and workshop language is ENGLISH
- All oral presentations, posters and
abstracts must be presented in English
- Until
autumn 2015 the PDF
download of the poster contributions are password
protected
and only accessible for Symposium and Workshop participants.
- Poster
will be
DIN A0 (portrait format
=> 120
cm/48
inch high x 90 cm/35 inch wide)
- Presentation
of every communication at the Conference is subjected to formal
registration
of at least one co-author.
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Symposium Talks:
(each
talk 30 min)
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- Monday
23rd of
March 10:00 a.m. to
6:00 p.m.
- Tuesday
24th of
March 8:30 a.m.
to 6:00 p.m.
- Wednesday 25th of
March
8:30 a.m.
to 5:00 p.m.
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Publish
your conference contribution as a 'full peer reviewed paper' in
"Biomolecular Detection and
Quantification"
Biomolecular Detection and Quantification (BDQ) is an open access,
peer-reviewed international journal dedicated to championing excellence
in molecular study design, measurement, data analysis and
reporting. Its focus is
on the application of qualitative and quantitative molecular
methodologies to all areas of clinical and life sciences.
A special BDQ issue with the title “Advanced
Molecular Diagnostics for Biomarker Discovery” will be
published in summer 2105. You can submit your conference contribution
(talk or poster) manuscript until 30th
April 2015.
Please submit
your manuscript here => http://ees.elsevier.com/bdq/default.asp?pg=preRegistration.asp
After REGISTRATION and LOGIN select "Special Issue (SI) Adv. Molecular
Diagnostics"
Download
BDQ papers on Elsevier Science Direct
The journal has two main
aims:
- to provide a forum for
discussion and recommendation of guidelines designed to improve the
accuracy of molecular measurement, its data analysis and the
transparency of its subsequent reporting;
- to publish molecular biology
based studies that adhere to best practice guidelines, both current and
future.
Benefits
of publishing open access with
Elsevier's
Biomolecular Detection and Quantification - www.journals.elsevier.com/biomolecular-detection-and-quantification/
- Peer-reviewed journal indexed by Scopus and
supporting ORCID and Crossref to help maintain your publication record.
- A renowned and
respected editorial team with
extensive knowledge of your research field. BDQ was established by a
group of scientists based on their experience developing and publishing
the Minimum Information for Publication of Quantitative Real-Time (and
Digital) PCR Experiments (the MIQE and digital MIQE guidelines).
- Reaching key audiences with 10 million active
users per month using our publishing platform ScienceDirect.
- Choice of user
licenses so
authors can decide how best to publish open access.
- A discount on the publication fee for
a limited time.
For more information about
publishing Open
Access with Elsevier, including funding body arrangements,
institutional agreements and more, visit: www.elsevier.com/openaccess
We look forward to receiving your paper until
31st March 2015!
Kind regards,
The Editors
Stephen Bustin, Jim
Huggett, Justin O'Grady, Michael W. Pfaffl, Carl Witwer, Ron Cook
Please
direct
your
enquiry
to our scientific organisation
team,
headed by Michael
W. Pfaffl qPCR-NGS-2015@wzw.tum.de
©
2014 - 2015
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