qPCR & NGS 2015 Event Advanced Molecular Diagnostics for Biomarker Discovery 7th international qPCR & NGS Event Symposium  &  Industrial Exhibition  &  Application Workshops 23 - 27 March 2015,   in Freising-Weihenstephan, School of Life Sciences, Technical University of Munich, Weihenstephan, Germany
Join our next event: qPCR dPCR & NGS 2017 8th international Gene Quantification Event 3rd - 7th April 2017 Liqid Biopsy, Integrative Big Data Analysis, Biomarker Signature ... and beyond First impressions from qPCR & NGS 2015 Event -- a trailer We welcome 480 participants from 38 countries Browse the conference agenda -- HTML version  AgendaHTML.qPCR-NGS-2015.net  and for printing PDF version  AgendaPDF.qPCR-NGS-2015.net ... ... and the Poster sessions -- printing PDF version of the poster presentations  Posters.qPCR-NGS-2015.net Poster Session 1 Poster Session 2 Poster Session 3
We present five qPCR, digital PCR and NGS Application Workshops on 26-27th March -- Workshops.qPCR-NGS-2015.net Register for the Symposium and the Application Workshops --  Registration.qPCR-NGS-2015.net Promotion Trailer qPCR & NGS 2015 -- YouTube -- Follow 200 talks from previous events on streaming server www.eConferences.de Download Event Brochure -- Event-announcement.qPCR-NGS-2015.net Call for conference contributions (Talk and Poster abstracts) deadline 6th Feb 2015 -- Call.qPCR-NGS-2015.net

Freising,  1st April 2015

Dear colleagues,
dear researchers,
dear company representatives,

The great international interest in the previous qPCR & NGS Events from 2004 till 2013 with a constant audience of more than 500 participants from all over the world motivates repeating the success next year in March 2015. We broaden our focus in genomics applications from quantitative RT-PCR, over digital PCR to the latest Next Generation Sequencing technologies. The date for the 7th International qPCR Symposium & Exhibition & Application Workshops is from the 23rd to the 27th March 2015. Parallel to the scientific symposium an industrial exhibition will take place where around 35-40 international companies will be presenting their newest qPCR, dPCR and NGS services and technologies. The symposium will be followed by various qPCR & NGS Workshops taking place March 26th and 27th powered by TATAA Biocenter, Qiagen, Genomatix and other leaders in the field.

Event location is the central lecture hall complex and the foyer at TUM (Technical University of Munich) in Freising Weihenstephan, Germany. The TUM and the Biotech region around Munich are part of the largest Biotech cluster in Europe, located close to the Munich airport (MUC) directly in the heart of Bavaria.

The focus of the qPCR & NGS 2015 Event is

Advanced Molecular Diagnostics for Biomarker Discovery

The symposium is based on 77 lectures and 76 posters presented by international recognised experts in their application fields. The emphasis will be on unbiased, didactic and scientific information exchange. One third of the talks will be presented by invited speakers, one third of the speakers will be selected from the submitted abstracts and one third will be given to qPCR and NGS company R&D representatives. Various poster sessions will be held in parallel in a separate poster exhibition hall. All scientific contributions will be published in an abstract book (qPCR & NGS 2015 Proceedings -- ISBN 978-3-00-048805-4). All talks will be recorded and made public in autumn 2015 via the www.eConferences.de streaming platform together with around 200 talks from qPCR 2010 onwards.

Leading academic researchers and industrial contributors in the field will participate in the symposium, which will be an arena for fruitful discussions between researchers of different backgrounds. The Symposium Talks, Poster Sessions, Industrial Exhibition and associated qPCR & NGS Application Workshops offer an overview of the present knowledge and future developments in qPCR, next generation sequencing and gene expression measurement technology and its wide applications in research.
As usual the qPCR & NGS Event is structured in three parts:		Download Event Flyer
1.    Symposium & Poster Sessions	taking place March  23-25  2015
2.    Industrial Exhibition with 40 companies       (exhibition is fully booked by 40 companies)	taking place March  23-25  2015
3.    qPCR & NGS Application Workshops	taking place March  26-27  2015
	Promotion Trailer qPCR & NGS 2015 Follow 200 talks from previous events on streaming server www.eConferences.de
Keynote Lectures: PCR in less than 30 seconds: Efficient, specific amplification with increased primer and polymerase. Carl T Wittwer; University of Utah, United States of America Digital PLA  (Proximity Ligation Assay) Stephen Bustin; Anglia Ruskin University, Chelmsford, Essex, United Kingdom Next-Generation RNA-Seq. Gary Schroth; Illumina, United States of America How could digital PCR benefit clinical analysis? Jim Francis Huggett; LGC, United Kingdom Updated evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study. Jo Vandesompele; Ghent University on behalf of the microRNA quality control study consortium, Belgium Mass Spectrometry Based Draft Of The Human Proteome. Bernhard Küster; TU München, Freising Weihenstephan, Germany Next Generation Sequencing Applications to the study of human and animal Microbiomes. Karen E. Nelson, J. Craig Venter Institute, United States of America Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer. Mikael Kubista; TATAA Biocenter, Sweden Variability of the Reverse Transcription Step: Practical Implications. Tania Nolan; The Gene Team, United Kingdom Decoding lncRNA functions using high-throughput pathway perturbation. Pieter Mestdagh; Biogazelle, Belgium
Please register for the Symposium & Workshops, using the Internet based ConfTool registration and submission platform => Registration.qPCR-NGS-2015.net
Publish your conference contribution in "Biomolecular Detection and Quantification" Biomolecular Detection and Quantification (BDQ) is an open access, peer-reviewed international journal dedicated to championing excellence in molecular study design, measurement, data analysis and reporting. Its focus is on the application of qualitative and quantitative molecular methodologies to all areas of clinical and life sciences. A special BDQ issue with the title “Advanced Molecular Diagnostics for Biomarker Discovery” will be published in summer 2015. You can submit your conference contribution (talk or poster) manuscript until 30th April 2015. Please submit your manuscript here => http://ees.elsevier.com/bdq/default.asp?pg=preRegistration.asp After REGISTRATION and LOGIN select  "Special Issue (SI)  Adv. Molecular Diagnostics" Download recent BDQ papers on Elsevier Science Direct

---

Symposium Talk and Poster sessions:

Main topic:   Advanced Molecular Diagnostics
This session is focusing on the application of highly sophisticated methods, application and algorithms to discover, detect, and validate molecular diagnostic markers. A special focus is lying on the integrative analysis of multi level biomarkers, e.g. microRNA – mRNA, integrative analysis of genomic, proteomic, metabolomic and phenotypic markers.

Main topic:   Biomarker Discovery
A session about the discovery, identification and validation of molecular biomarkers:  diagnostic, prognostic or therapeutic markers on DNA, RNA, microRNA or small RNA, metabolome and proteome level (e.g. disease markers, cancer or stem-cells markers, tissue specific markers, differentiation markers, methylation markers). New assay systems will be presented like high throughput proteomics (MS based proteome map), PLA (Proximity Ligation Assay) or PEA (Proximity Elongation Assay).

Main topic:   Next Generation Sequencing (NGS)
NGS applications offers new holistic analysis of any kind of nucleic acid, to investigate the Genome, Exome, Epigenome, Transcriptome and Splicome (total RNA, microRNA, small RNA Seq, long ncRNA), CHIP purified nucleic acids, etc.
Various NGS sub-sessions will be presented:
•    NGS overview talks - information technology in the era of NGS
•    Pre NGS - sample prep & setup & library generation
•    NGS – new sequencing technologies (e.g. single molecule and pore sequencing)
•    NGS – data analysis (data management, mapping, alignment algorithms, data de novo assembly )
•    NGS – diagnostic applications

MicroGenomics & Single-cells diagnostics
Focus is on micro-genomics, the application of molecular methods to detect biomarkers in a minimal amount of tissue or matrix. New applications in single-cell isolation, separation or characterisation technologies, laser micro dissection, isolating circulating tumour cells (CTC), pre-amplification techniques, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection, , single-cell handling, FACS sorting and spotting.

Circulating Nucleic Acids
A new class of biomarkers are circulating nucleic acids (DNA, RNA, small RNAs, and long ncRNAs) which are free floating (?), bound to proteins or covered in micro-vesicles. One focus will be on exosome or micro-vesicles isolation, purification for bio-liquids, amount and size quantification, and characterisation techniques, including intra-cellular and membrane bound protein or lipid markers.

Molecular Diagnostics in Agriculture, Veterinary Medicine, Food & Environmental Science
This session is dedicated to molecular diagnostics in Life Science, with focus on Agriculture, Veterinary Medicine, and Environmental Science (which is the major research focus here at the TUM campus in Weihenstephan). All applications of highly sophisticated quantification methods, field application or algorithms to research the wide field of agro-veterinarian life science are very welcome.

MIQE & QM & Standardisation strategies in molecular diagnostics
This session is focusing on standardisation strategies and quality management in molecular diagnostics. The goal is to guarantee better and more valid results. Of special interest in the context of qPCR are the MIQE guidelines (minimum information for publication of quantitative real-time PCR experiments). Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.

Digital PCR  &  Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and clonally amplify nucleic acids including DNA, cDNA, mRNA or microRNA. It allows a more reliable collection and highly sensitive measurement of nucleic acid amounts, applications in copy number variants, point mutations in molecular diagnostics.

Non-coding RNAs -- microRNA, small RNAs, long non-coding RNAs
This session is dedicated to the family of non-coding RNA and its RNAi mechanism and applications:  extraction of non-coding RNAs, RT-qPCR technologies to detect microRNA, long-non coding RNAs and new classes of small RNAs, siRNA knock down applications, microRNA targets and microRNA precursors, new siRNA manipulation, etc.

qPCR Data Analysis -- BioStatistics & BioInformatics
The data analysis in RT-qPCR expression profiling experiments is still challenging and time consuming. From the literature it is well known, that a lot of error is introduced by wrong experimental study design, reference gene normalisation or qPCR data analysis. This session is focusing on useful algorithms and software applications for data mining, calculation of relative expression, primer and probe design for any kind of nucleic acid (mRNA, microRNA, piRNA), real-time PCR efficiency determination, mathematical modelling, multivariate expression profiling raw data analysis, statistics in real-time PCR, data management, multi-way expression profiling, multiple regression analysis, 3D data visualization, and much more!

---

The scientific organization is managed by international well-known scientists:
Scientific board:
Stephen Bustin	Prof. of Molecular Medicine, Faculty of Health, Social Care & Education, Anglia Ruskin University, UK
Mikael Kubista	Prof. of Biotechnology, BTU, Czech Academy of Sciences & TATAA Biocenter, Sweden
Vladimir Benes	PhD, Head of the Genomics Core Facility at EMBL, Heidelberg, Germany
Jim Huggett	PhD, Science Leader, Nucleic Acid Metrology, LGC, London Twickenham, UK
Jo Vandesompele	Prof. at the Center of Medical Genetics, University of Ghent, Belgium
Michael W. Pfaffl	Prof. of Molecular Physiology, TUM School of Life Sciences, Weihenstephan, Germany Scientific coordinator of the Symposium and the Application Workshops  qPCR-NGS-2015@wzw.tum.de
Event organization:	Sylvia Pfaffl,   bioMCC,   85354 Freising    Eventmanagement@bioMCC.com

 
We are looking forward to meeting you in March 2015 at the Symposium in Freising-Weihenstephan