1st International qPCR
Symposium & Application Workshop ©
Transcriptomics, Clinical
Diagnostics & Gene Quantification
3rd -
6th March, 2004 in Freising-Weihenstephan, Germany
Welcome &
Wednesday talks
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Thursday Talks
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Friday Talks
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Application Workshop
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Industrial Exhibition
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Evening Event in Bräustüberl
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Evening Event in Lindenkeller
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Catering Team
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Industrial Exhibition
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Wednesday Evening
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Bayerische Gemütlichkeit
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Thursday Lectures
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1st International qPCR
Symposium & Application Workshop
3rd - 6th March,
2004 in Freising-Weihenstephan, Germany
The Symposium presents unbiased international lectures & posters
about quantification strategies, normalisation methods & new
algorithms for exact nucleic acid quantification in the broad range of
qPCR applications:
Transcriptomics
Clinical Diagnostics
Food
Hygiene & GMO
Expression
profiling & cDNA
Array Verification
Microbiology &
Virology
NutriGenomics
& PharmacoGenomics
Theoretical qPCR & real-time PCR efficiency
Pre-analytical Steps
& RT & Isolation of Nucleic Acids
NEWS - NEWS - NEWS - NEWS -
NEWS - NEWS - NEWS - NEWS - NEWS - NEWS
Application Workshop is
FULLY booked with 80 participants !!!
More than 32
companies will
participate in the Industrial Exhibition along with the
Symposium.
(real-time PCR Cyclers, Kit Producers, Nucleic Acid
Purification Systems, Enzyme Producers, Plasticware Suppliers,
Software Developers, etc.)
http://www.wzw.tum.de/gene-quantification/qpcr2004/companies.html
More
than 65 internationally reknown speakers and over 135
poster contributions
in 8 specialised poster sessions will participat in a lively
and exciting programme enabling
the valuable exchange of information in the qPCR field.
465 participants and around 200 abstracts
from every place on earth were send to us.
!!! Flag
order is randomised !!!
Invitation to the 1st
International
qPCR Symposium & Application Workshop
Dear researchers and colleagues,
On behalf of
the Organisation Committee and the Scientific Board it is great
pleasure to invite you to the 1st International qPCR Symposium &
Application Workshop to be held March 3-6, 2004, at the Center of Life
Science in Freising Weihenstephan, Technische Universität
München (Germany http://www.wzw.tum.de/wzw/english/index.html).
The
polymerase chain reaction (PCR) is the most sensitive technique
to detect low abundance mRNA and minute amounts of DNA typically
present in tissue samples. Real-time reverse transcription PCR (qPCR or
kinetic RT-PCR) is rapidly becoming the method of choice for
quantitative gene expression analysis and molecular diagnostics owing
to its high sensitivity, excellent reproducibility, and wide dynamic
quantification range. However, this ultra high sensitivity makes qPCR
sensitive to experimental error, variations in experimental protocols
and presence of contaminants in
complex biological samples. Successful applications of qPCR require
understanding of the practical problems associated with sample
handling, including careful experimental design, assay optimisation,
appropriate validation and proper data treatment.
This 1st International qPCR
Symposium will focus on all aspects of qPCR technology and its
applications in research and diagnostics. Leading academic researchers
and industrial contributors in the field will be participate in the
symposium, which will be an arena for fruitful discussions between
researchers of different backgrounds. The Symposium and associated
Application Workshop offers an overview of the present knowledge and
future developments in qPCR technology and its wide applications.
The Application Workshop
offers hands-on training by leading experts from TATAA Biocenter (www.tataa.com) in
qPCR. Major probe technologies will be demonstrated, leading and
emerging instrument platforms will be presented, and advanced primer
and probe design programs will be available. Hands-on training will be
provided on quantitative gene expression, including design and
optimization of both RT and qPCR protocols.
Michael W. Pfaffl
Mikael Kubista
(Chairman Symposium)
(Chairman
Application Workshop)
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Cordial
Greetings
from Kary B. Mullis
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Dear Dr. Pfaffl,
I am sorry that I will not be
able to
attend the First International qPCR Symposium and Application
Workshop. Please deliver my cordial greetings to those in
attendance and remind all that “symposium” is from the Greek “symposion”
meaning to drink together. One shouldn’t have to walk too far in
Bavaria to find a stein worth raising in celebration of "Twenty
years of PCR” without which we may have had another twenty
years without PCR. We’d already had three billion years of that,
and it was getting tiresome. So don’t forget to go to the
sessions, but do remember why you are there. Sympose !
Sorry I’m not there with you.
Cordially, Kary B. Mullis
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Confirmed Speakers:
Stephen Bustin
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Reader in Molecular Medicine,
School of Medicine, London
"Pitfalls in the quantification of
RNA using real-time RT-PCR."
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Mikael Kubista
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TATAA Biocenter, Gothenborg ,
Sweden
"Real-time
PCR is the most sensitive technique for biomolecular detection.
Possibilities and Limitations in Research and in Clinical Diagnostics."
(scientific coordination of
the Application workshop)
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Udo Reischl
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Institute of Medical Microbiology
& Hygiene, Regensburg, Germany
"LightCycler
Applications in Diagnostic Bacteriology."
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Heinrich H.D. Meyer
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Physiology - Weihenstephan, ZIEL, Freising, Germany,
Center of
Food & Life Science-Weihenstephan
"qPCR and Transcriptomics:
Creation of a new tool to understand life."
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Michael W. Pfaffl
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Physiology - Weihenstephan, ZIEL, Germany
"Determination of real-time PCR efficiency -
An overview of different methods."
(scientific coordination of the
Symposium)
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Confirmed Speakers
from University:
Carl T. Wittwer
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University of Utah
School of Medicine
"High Resolution Melting Curve Analysis." |
Lawrence Wangh
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Brandeis University, Boston, MA,
USA
"LATE-PCR and Allied Technologies
for Amplification and Utilization of Single-stranded DNA."
and
"PurAmp
- a New Quantitative
Method for Preparation, Synthesis, and Amplification of Both cDNA and
Genomic DNA in a Single Tube."
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David Whitcombe
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DxS Genotyping
"Scorpions-
Application in Genotyping and Real time PCR."
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Hannelore Daniel
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Molecular Nutrition Unit, ZIEL,
Germany Center of Food & Life
Science
"NUTRIGENOMICS:
the road that leads to new insights into nutritional processes."
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Anders Stahlberg
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Chalmers University, Sweden
"Quantitative gene expression
analysis by Real-time
PCR - How to optimize the reverse transcription and real-time PCR
reactions."
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Pamela Pinzani
&
Mario
Pazzagli
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Clinical Biochemistry Unit, Dep.
of Clinical Physiopathology Florence, Italy
"Laser
capture microdissection and real-time PCR in Human Cancer." |
Deborah Grove
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Pennsylvania
State University, USA
"Real time PCR in a Core Facility:
Helping others to help
themselves."
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Jo Vandesompele
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Center of Medical Genetics, Ghent
University, Belgium
"Accurate normalization of gene
expression using multiple internal control genes."
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Kristin Brevik Andersson
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Institute of Experimental Medical
Research, Oslo
"Normalization
genes for heart failure."
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Jim Huggett
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Center for Infectious Diseases,
London, UK
"Validation
of housekeeping genes for normalising RNA expression."
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Confirmed Speakers sponsored by the
Industry:
Thomas Fröhlich et al. |
Platinum
Sponsor
Roche
Diagnostics R
& D LightCycler Development Group
"Genotyping
of SNPs via Fluorescent Melting Curve Analysis."
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Hilary Srere
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Gold Sponsor
R
& D Bio-Rad Laboratories
"Relative Gene Expression
Studies using Multiplex Quantitative PCR on the
Bio-Rad iCycler iQ Real Time PCR Detection System."
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Babette Fahey
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Gold Sponsor
MJ
Research Inc.
"Real-time Detection
and Quantitation of Genetically Modified Soy."
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Manohar Furtado
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Applied
Biosystems R
& D -
Applera Deutschland GmbH
"Expression Profiling of
Candidate Genes: Assays-on-Demand(tm) Gene
Expression products based
on TaqMan MGB chemistry."
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Debra Nickson
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Invitrogen
Europe
"LUX™
Fluorogenic Detection System and other new
approaches in qPCR."
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Dirk
Löffert
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Silver Sponsor
Qiagen
"Novel amplification and
detection chemistries for real-time PCR."
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Thomas
Köhler
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Silver Sponsor
Roboscreen,
Leipzig
"Standardized gene expression
profiling and tumor prognosis."
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Tania Nolan
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Silver Sponsor
Stratagene
Europe
"A Housekeeping-Gene Free Zone for
Normalisation."
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Tobias
Ruckes
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Silver Sponsor
Artus
GmbH
“Pitfalls in transfer of
diagnostic duplex qPCR assays between technological platforms.” |
Thomas Kaiser
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Corbett Research R&D,
Australia sponsered
by Biotage ( Pyrosequencing )
"Different
approaches of data analysis in real-time amplification."
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Hans Nitschko
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Max-von-Pettenkofer Institut, LMU sponsored
by Abgene
"Genotyping and
quantification of hepatitis C virus using
fluorescent probes."
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Martin Lee
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BioGene Limited
"Efficient non-linear
analysis of kinetic amplification for quantification and automated
results
calling."
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Stephen Sharp
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Sequenom
"Highly
sensitive analysis of allele-specific gene expression by MALDI-TOF MS."
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Khalil Arar
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Director of Research &
Development PROLIGO SAS
"LNA
probes, a new tool to enhance your Real Time QPCR applications."
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Important Dates:
The official conference and workshop language is ENGLISH.
No simultaneous translation will be provided.
All oral presentation, poster and abstarcts must be presented in ENGLISH.
Abstract
submission deadline:
Last
minute POSTER CALL - until 31. January 2004 - CLOSED
poster-call.pdf
Abstract submission:
qpcr2004@wzw.tum.de
Guidelines for abstract submission
www.wzw.tum.de/gene-quantification/qpcr2004/abstract.pdf
Please indicate if you want to present either a Poster or give an oral
Presentation via MS PowerPoint.
Symposium Publications:
Abstracts
The symposium proceedings including all Abstract, Posters and Oral
Presentations and will be published on the Symposium & Gene
Quantification Homepage. Until August 2004 the PDF download of the
contributions is password protected and only accessible for Symposium
or Application Workshop participants www.wzw.tum.de/gene-quantification/qpcr2004/abstracts-a.html
Full Papers /
Reviews / Minireviews
Symposium organisation & registration:
Please sent registartion forms to susanna.wirth@tumtech.de
Registration Form (PDF) www.wzw.tum.de/gene-quantification/qpcr2004/registration.pdf
Registration
Form (DOC) www.wzw.tum.de/gene-quantification/qpcr2004/registration.doc
Please send your registration form via:
Snail mail Dr.
Susanna Wirth, TUM-Tech, Saarstraße
7, D-80797 München, Germany
or
FAX
Dr. Susanna Wirth,
TUM-Tech, FAX No. +49-89-306695-66
and e-mail the completed
Word document
Please sent
registartion forms to susanna.wirth@tumtech.de
Symposium Registration Fees (3 days):
(incl. MWST = VAT)
early registration fee
(before 15th Nov. 2003)
360 €
late registration fee
(after 15th Nov. 2003):
430 €
Application Workshop Registration Fee (1.5 days):
(incl. MWST = VAT)
Application
Workshop is FULLY booked with 80 participants !!!
registration fee
(before 15th
Nov. 2003) 430 €
late
registration fee
(after 15th Nov. 2003):
490 €
Symposium & Application Workshop Registration Fee
(4 days):
(incl. MWST = VAT)
Application
Workshop is FULLY booked with 80 participants !!!
registration fee
(before 15th
Nov. 2003) 790 €
late
registration fee
(after 15th Nov. 2003):
920 €
Please transfer the registration fee to
the bank account:
Payment is possible by Bank Transfer in Euro [ € ] and free
of charges to the recipient TUM-Tech
Account
No. 10374304
Kreissparkasse München Starnberg Code 702 501 50
International Bankcode:
iBAN: DE75702501500010374304
Swift –Adresse
BYLADEMM
BIC-Code
BYLADEM1KMS
Please state your
Name and Symposium or Workshop or Symposium
& Workshop
WE ARE FULLY BOOKED
!!!
32 companies will participate in the Industrial
Exhibition.
Industrial Exhibition conditions
http://www.wzw.tum.de/gene-quantification/qpcr2004/companies.html
For details for the
industrial exhibition, e.g. technical management, stand allocation,
electronic installations, exhibition opening times, costs, symposium
sponsoring etc. please contact Dr. Susanna Wirth at
TUM-Tech GmbH (susanna.wirth@tumtech.de)
Access to the Symposium:
http://www.wzw.tum.de/gene-quantification/qpcr2004/access.html
Accomodation:
Please make your own reservations via http://www.freising.de/tourismus/hotelliste.htm
Hotels (***) Hotel Lerner, Hotel Bayerischer Hof, Hotel
Am Klostergarten, Hotel zum Löwen, Isarhotel
Hotels (**** - *****) Dorint Hotel
Freising, München Airport Marriott Hotel
Meals and beverages:
Substantial Welfare - Balancing
your mind and body welfare.pdf
Map to Dinner - 1st
March
Registration
fees including all snacks, meals, and beverages (coffee, tea,
non-alcoholic during the
lectures & alcoholic drinks at diner) during the Symposium and
the Application Workshop.
Noontime a lunch is served in the student cafeteria. At Wednesday
evening a Bavarian Dinner Buffet is served
in the center of the industrial exhibition. This will stimulate the
scientific conversation between participants from academic institutions
and private companies and will offer opportunities for further research and collaborations.
On Thursday evening a Bavarian Gala Banquette in served in the
traditional beer
cellar of
the "Oldest
brewery of the world“, directly on the campus WEIHENSTEPHAN, founded
1040 (www.brauerei-weihenstephan.de)
or alternatively an Mediterranean Gala Dinner at Pasta
& More (Lindenkeller)
http://www.essen-macht-spass.de/Essen-macht-spass_page8.htm
If you like vegeterian or pork free meals, please indicate it at
the registration form or at the registration desk.
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