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qPCR Symposium Proceedings 
ISBN 3-00-013404-2
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qPCR Symposium Agenda
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Oral Presentations
Oral presentations PDFs can be downloaded
in the password protected area.
http://qPCR2004.gene-quantification.info/pub/pub-main.html#oral
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Wednesday
3 March 2004
Welcome & Opening
of the Symposium
Lecture hall HS 14
08:00 – 10:00
Built-up for Industrial Exhibition
10:00 – 11:30 Arrival
& Registration
11:30 – 12:30 Get-together lunch in the student cafeteria
12:30
“Welcome & Opening of the Symposium.”
Michael W. Pfaffl &
Mikael Kubista
Scientific coordination of the Symposium
& Workshop
12:45
“Welcome at the Center of Food & Life Science in Freising
Weihenstephan.”
Bertold Hock, Dean of the Center
of Food & Life Science-Weihenstephan, Freising, Germany
13:00
"qPCR and Transcriptomics: Creation of a new tool to understand
life."
Heinrich H. D. Meyer, Physiology
- Weihenstephan, Freising, Center of Food & Life Science-Weihenstephan,
Germany
13:45
"Pitfalls in the quantification of RNA using real-time
RT-PCR.” ( 50 MB )
Stephen Bustin, Reader in Molecular
Medicine, School of Medicine, London
14:30 – 15:00 Coffee break
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Session: Pre-Analytical Steps
Chairs: M. Kubista &
D. Grove
Lecture hall HS 14
15:00
Session introduction by M. Kubista & D.
Grove
15:10 "Quantitative
gene expression analysis by Real-time PCR - How to optimize
the reverse transcription and real-time PCR reactions:”
Anders Stahlberg, Mikael Kubista,
Chalmers University, Sweden
15:40
“Overcoming bias in quantitative RT-PCR: Different methods
of reverse transcription influence sensitivity and accuracy
of gene expression patterns.”
Ginzinger DG1, Yu M1, Schuster D2
& A Rashtchian2; University of California San Francisco,
Comprehensive Cancer Center, Genome analysis core facility,
San Francisco, CA, USA; 2 Quanta BioSciences, Inc. Gaithersburg,
MD 20877, USA
16:00 “RNA Integrity
Number (RIN) –Standardization of RNA Integrity Measurements.”
Odilo Mueller1, Andreas Schroeder1,
Samar Lightfoot2, Ruediger Salowsky1, Susanne Stocker1,
Thomas Ragg3;
1 Agilent Technologies, Waldbronn,
Germany; 2 Agilent Technologies, Palo Alto, USA; 3 Quantiom
Bioinformatics, Weingarten, Germany
16:20
“Gene expression quantitated in PAXgene™ frozen stored
blood as compared to freshly Immuno Magnetic Separated (IMS)
blood cells.”
Ovstebo R, Haug KBF, Kierulf P.;
The Research and Developement Group, Department of Clinical
Chemistry, Ulleval University Hospital, Oslo, Norway
16:40
“Modified silica-magnetite composite as a universal matrix
for nucleic acids isolation.”
Zhao X, Huang Z,
Luan G; Biovision Biotech, Inc, China
17:00 – 18:00
Poster session
18:00 – 22:00 Get-together
with the Companies Bavarian Dinner Buffet in the
Aula
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Thursday
4 March 2004
Session: qPCR Application in
Clinical Diagnostics
Chairs: S. Bustin &
M. Pazzagli
Lecture hall HS 14
08:30
Session introduction by S. Bustin & M.
Pazzagli
08:40
"Real-time PCR is the most sensitive technique for biomolecular
detection. Possibilities and Limitations in Research and
in Clinical Diagnostics."
Mikael Kubista, Chalmers University
of Technology and the TATAA Biocenter, Göteborg, Sweden
09:10
"Laser capture microdissection and real-time PCR in Human
Cancer."
Pamela Pinzani, Prof. Mario Pazzagli,
Clinical Biochemistry Unit, Dep. of Clinical Physiopathology
Florence, Italy
09:40
Microarray data
of pooled samples compared to real-time-PCR of individual
samples in African Children with Malaria
Yvonne Kalmbach (1),
Angelica B. W. Boldt (1), Martin P. Grobusch (1,2), Cristina
Tena-Tomás (1), Arnaud Dzeing (4), Maryvonne Kombila
(4), Michael Bonin (3), Olaf Riess (3), Peter G. Kremsner (1,2),
Jürgen F. J. Kun (1).
Institute for Tropical Medicine, Dept. of Parasitology,
Wilhelmstr. 27, D-72074, Tübingen, Germany.
Medical Research Unit, Albert
Schweitzer Hospital, Lambaréné, Gabon.
Institute for Human Genetics,
Dept. of Medical Genetics, Calwerstr. 7, D-72076, Tübingen,
Germany
Département de Parasitologie,
Faculté de Médicine, Libreville, Gabon.
10:00 “Quantitative
analysis of gene expression – a valuable tool in clinical
immunology.”
Giese T1, Stallmach A2, Zeier M3
& Meuer SC1; 1Institute of Immunology, University Hospital
Heidelberg; 2Dept. Gastroenterology, Catholic Hospital Essen-Nord;
3Dept. Nephrology, University Hospital Heidelberg
10:20 – 10:45 Coffee
break
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Session:
qPCR Application in Microbiology & Virology
Chairs: U. Reischl &
H. Nitschko
Lecture hall HS 14
10:45
Session introduction by U. Reischl & H.
Nitschko
10:50 "LightCycler
Applications in Diagnostic Bacteriology."
Udo Reischl, Institute of Medical
Microbiology & Hygiene, Regensburg, Germany
11:20
"Genotyping and quantification of hepatitis C virus using
fluorescent probes."
Hans Nitschko, Max-von-Pettenkofer
Institut, LMU; Munich, Germany sponsored by Abgene
11:50
“Dissection of the retroviral life cycle using real-time
PCR assays.”
Klein D, Nosek D, Leichsenring B
& Knapp E; Institute of Virology, University of Veterinary
Medicine Vienna, Austria
12:10 “Real-time
quantitative PCR assays for the detection and monitoring
of pathogenic human viruses in immunosuppressed pediatric
patients.”
Suda Magdalena, Matthes-Martin Susanne
and Lion Thomas; Div. Mol. Microbiology and Development
of Genetic Diagnostics, Children´s Cancer Research Institute,
Vienna
12:30 - 13:30 Lunch
in the student cafeteria
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Session:
Normalization & Standardization
Chairs: T. Köhler
& M. W. Pfaffl
Lecture hall HS 14
13:30
Session introduction by T. Köhler &
M.W. Pfaffl
13:40 "Accurate
normalization of gene expression using multiple internal
control genes."
Jo Vandesompele, Center of Medical
Genetics, Ghent University, Belgium
14:10
"Normalization genes for heart failure."
Kristin Brevik Andersson, Institute
of Experimental Medical Research, Oslo
14:40
"Validation of housekeeping genes for normalising RNA
expression.”
Jim Huggett, Center for Infectious
Diseases, London, UK
15:10 – 15:40 Coffee
break
15:40
"Relative Gene Expression Studies using
Multiplex Quantitative PCR on the Bio-Rad iCycler iQ Real
Time PCR Detection System."
Hilary Srere, R & D Bio-Rad Laboratories,
Hercules, CA, USA
16:10
"Standardized gene expression profiling and tumor prognosis."
Thomas Köhler, Roboscreen, Leipzig,
Germany
16:50
"A Housekeeping-Gene Free Zone for Normalization."
Tania Nolan, Stratagene Europe, Amsterdam,
The Netherlands
17:10 “Housekeeping
gene expression in human seminoma and normal testicular tissue.”
Neuvians TP, Sauer CG, Bleyl U &
Grobholzer R; Pathologisches Institut, Universitätsklinikum
Mannheim der Rupprecht-Karls-Universität Heidelberg,
Deutschland
17:30
“Pitfalls in transfer of diagnostic duplex qPCR assays
between technological platforms.”
Tobias Ruckes, Artus GmbH, Hamburg,
Germany
17:50 – 19:00 Poster session
19:00 – 22:00 Bavarian
Gala Dinner in the „Oldest brewery of the world” WEIHENSTEPHAN
or alternatively
19:00 – 22:00 Mediterranean
Gala Dinner in LINDENKELLER
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Thursday
4 March 2004
Session: New Approaches in
qPCR & Automatisation
Chairs: C. Wittwer &
L. Wangh
Lecture hall HS 15
08:30
Session introduction by C. Wittwer & L.
Wangh
08:40 "Real time
PCR in a Core Facility: Helping others to help themselves."
Deborah Grove, Pennsylvania State
University, USA
09:10
"Determination of real-time PCR efficiency - An overview
of different methods."
Michael W. Pfaffl, Physiology - Weihenstephan,
ZIEL, Center of Food & Life Science-Weihenstephan Germany
09:40
"LATE-PCR and Allied Technologies for Amplification and
Utilization of Single-stranded DNA."
Lawrence Wangh, Brandeis University,
Boston, MA, USA
10:10
"Expression Profiling of Candidate Genes: Assays-on-Demand
Gene Expression products based on TaqMan MGB chemistry."
Manohar Furtado, Applied Biosystems R & D -
Applera Deutschland GmbH, Germany
10:30 – 11:00
Coffee break
11:00
“Establishment and the use of multiplex applications.”
Böll Inga, Roche Applied Science,
Penzberg, Germany
11:20
"Efficient non-linear analysis of kinetic amplification
for quantification and automated results calling."
Martin Lee, BioGene Limited, Kimbolton,
UK
11:40
“Quantitative PCR – a novel tool for protein quantification.”
Andreas Kage1, Wolfgang Henke2, Heiko
Witt3, Claudia Dahmen4 ; 1 Charité - Universitätsmedizin
für Berlin, Institut für Laboratoriums-medizin
und Pathobiochemie, 2 Charité - Universitätsmedizin
für Berlin, Institut für Laboratoriumsmedizin
und Pathobiochemie, 3 Charité - Universitätsmedizin
für Berlin, Abt. für Pädiatrie, Augustenburger
Platz 1, 13353 Berlin; 4 AptaRes AG, Im Biotechnologiepark
TGZ 1, 14943 Luckenwalde, Germany
12:00
"PurAmp - a New Quantitative Method for Preparation, Synthesis,
and Amplification of Both cDNA and Genomic DNA in a Single
Tube."
Lawrence Wangh, Brandeis University,
Boston, MA, USA
12:20
“A sensitive method for the quantitation of residual DNA
using Alu based sequences and real-time PCR amplification.”
Nussbaum O, Oppenheimer-Shaanan Y,
Eren R, Dagan S, Zaubermann, A; XTL Biopharmaceuticals
Ltd., Rehovot, Israel
12:40 - 13:40
Lunch in the student cafeteria
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Session:
Transcriptomics & Expression profiling
Chair: M. Kubista
Lecture hall HS 15
13:40
Session introduction by M. Kubista
13:50 "Different
approaches of data analysis in real-time amplification."
Thomas Kaiser, Corbett Research R&D,
Australia sponsered by Pyrosequencing, Sweden
14:20
“Real-Time RT-PCR profiling of over 1,400 Arabidopsis transcription
factors: Unprecedented sensitivity reveals novel root- and
shoot-specific genes.”
Czechowski T, Bari R, Stitt M, Scheibele
WR & Udvardi MK; Max-Planck Institute of Molecular
Plant Physiology, Am Mühlenberg 1, 14476 Golm, Germany
14:40 “PCR amplification
efficiency, relative quantification and the analysis of
alterations in cellular gene expression patterns.”
Petrauskene OV, MacLean J, Wong a,
& Furtado MR; Applied Biosystems, 850 Lincoln Center
Dr. Foster City, CA, USA
15:00
“A rapid method to measure reactivity of brain regions
upon stress exposure employing real-time qPCR analysis of
activity regulated gene transcripts and calculation of recommended
sample size.”
Koya E1, Spijkers S1, Homberg J2,
Schoeffelmeer ANM2, De Vries TJ2, Smit AB1; 1 Department
of Molecular and Cellular Neurobiology, Vrije Universiteit Amsterdam,
The Netherlands; 2. Department of Medical Pharamcology, VU-Medical
Center, The Netherlands
15:20 – 15:50
Coffee break
15:50
“Using real-time quantitative RT-PCR to validate a transcriptomics
analysis advancing embryo-maternal communication.”
Ulbrich S1), Bauernsachs S2), Rehfeld
S2), Mallok S3), Prelle K1), Wenigerkind H4), Blum H3) ,Wolf
E2) & Einspanier R1,5); 1Institute of Physiology, TUM,
Freising, Germany; 2Institute of Molecular Animal Breeding, LMU,
Munich, Germany; 3Gene Center of the LMU Munich, 4Bavarian Research
Center for Biology of Reproduction, Ober-schleissheim, Germany;
5 present address: Institute of Veterinary Biochemistry, Free University
of Berlin, Berlin, Germany
16:10
“Multiplex BD QZyme Assays – a reliable real-time qPCR
chemistry for analyzing the effects of gene silencing models.”
Larsen Robert, BD Biosciences, Belgium
16:30
“Human Transcriptome Probe Library - detecting 37,000
genes with 90 probes.”
Mouritzen P, Tolstrup N, Ramsing
NB; Exiqon A/S, Bygstubben 9, DK2950 Vedbaek, Denmark
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Session:
Quality Assessment in qPCR
Chair: T. Bar
Lecture hall HS 15
16:50
Session introduction by T. Bar
17:00 "High Resolution
Melting Curve Analysis."
Carl T. Wittwer, School of Medicine,
University of Utah, USA
17:30 “Comparative
Quality Assessment (CoQA) for real-time PCR.”
Tzachi Bar1 Neven Zoric2, Anders
Muszta3 and Mikael Kubista1, 2; 1Department of Chemistry
and Biosciences, Chalmers University of Technology, Medicinargatan,
2TATAA Biocenter, Medicinargatan, 3Department of Mathematical
statistics, Eklandagatan 86, 412 96, Gothenburg, Sweden
17:50
“An Italian external quality control program for quantitative
PCR assay based on the use of TaqMan probes: results of a 42
laboratory survey.”
Orlando C1, Casini Raggi C1, Pinzani
P1, Simi L1, Verderio P2, Marubini E2, Pazzagli M1. 1Clinical
Biochemistry Unit, Department of Clinical Physiopathology,
University of Florence, Italy; 2Operative Unit of Medical Statistics
and Biometry, European Institute of Oncology, Milan, Italy
18:10
“Data processing in real time PCR.”
Larionov A.A., Miller W.R.; Breast
Unit Research Group, Western General Hospital, Edinburgh,
UK
18:30 – 19:00 Poster
session
19:00 – 22:00 Bavarian
Gala Dinner in the „Oldest brewery of the world” WEIHENSTEPHAN
or alternatively
19:00 – 22:00
Mediterranean Gala Dinner in LINDENKELLER
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Session: Nutrigenomics
Chair: H. Daniel
Lecture hall HS 14
08:30
Session introduction by H. Daniel
08:40 "Nutrigenomics:
the road that leads to new insights into nutritional processes."
Hannelore Daniel, Molecular Nutrition
Unit, ZIEL, Center of Food & Life Science Weihenstephan,
Germany
09:10
“Nuclear Factors and Cytokines control TFFs down regulation
in tumor cell lines of the digestive tract.”
Baus-Loncar M, Dossinger V, Blin
N, Gött P & Kayademir T; Institute for Anthropology
and Human Genetics, Division of Molecular Genetics, University
of Tübingen, Germany
09:40
“Differential regulation of the sodium-ascorbate co-transporters
SVCT1 and SVCT2 expression in glutathione depleted CaCo-2
cells as assessed by functional analysis and quantitative real-time
PCR.”
Maulen NP1, Kempe S2, Nualart F3,
Bustamante ME1 & Vera JC2; 1Laboratory of Molecular
Biology, Faculty of Medicine, Universidad Católica de
la Santísima Concepción; 2Department of Physiopathology,
3Department of Cell Biology, Faculty of Biological Sciences,
University of Concepción, Concepción, Chile
10:00 – 10:30 Coffee
break
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Session:
Food Hygiene & GMO
Chair: Heinrich H. D. Meyer
Lecture hall HS 14
10:30
Session introduction by Heinrich H.D. Meyer
10:40 "Real-time
Detection and Quantitation of Genetically Modified Soy."
Babette Fahey, MJ Research Inc.,
Waltham, MA, USA
11:10 "Detection
and quantitation of GMO in official food and feed control."
S. Pecoraro, Bavarian Health and
Food Safety Authority Oberschleißheim, Germany
11:30
“CaMV virus detection with Real-time PCR – identification
of false positive results in 35S screening for genetically
modified organisms (GMOs).”
Cankar K, Gruden K, Tusek M, Toplak
N, Ravnikar M, Žel J; National Institute of Biology, Department
of Plant Physiology and Biotechnology, Večna pot 111, 1000
Ljubljana, Slovenia
11:50
“Finding the traces - real-time PCR assays for quantitative
and qualitative detection of animal DNA in food and feed.”
Bruns U, Müller M, Steinbohrn
R & Müller S; Institute of Animal Breeding and
Genetics, Veterinary University Vienna, Austria
12:10
“qPCR and Small Grain Cereals: Species and Transgene Detection.”
Terzi V1, Shewry PR 2, Stanca AM
1, Faccioli P 1; 1 Istituto Sperimentale per la Cerealicoltura,
Via San Protaso 302, 29017-Fiorenzuola d’Arda (PC) , Italy;:
2 IACR-Long Ashton Research Station, Long Ashton, Bristol BS18
9AF, UK
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Session: Detection methods
Chair: David Whitcomb
Lecture hall HS 15
08:30
Session introduction by D. Whitcombe
08:40 "Scorpions-
Application in Genotyping and Real time PCR."
David Whitcombe, DxS Genotyping,
Manchester, UK
09:10
"Genotyping of SNPs via Fluorescent Melting Curve Analysis."
Thomas Fröhlich, Roche Diagnostics
R & D LightCycler Development Group, Penzberg, Germany
09:40
“Real-time PCR genotyping using strand displacement probes.”
Li Q, Cheng J, Zhang Y, Molecular
Diagnostics Laboratory, Xiamen University, China
10:00 – 10:30 Coffee
break
10:30
"LUX Fluorogenic Detection System and other new approaches
in qPCR."
Debra Nickson, Invitrogen Europe
11:00 "LNA probes,
a new tool to enhance your real Time QPCR applications."
Khalil Arar, Proligo SAS, Paris,
France
11:30 "Novel amplification
and detection chemistries for real-time PCR."
Dirk Löffert, Qiagen, Hilden,
Germany
12:00 "Highly sensitive
analysis of allele-specific gene expression by MALDI-TOF
MS."
Stephen Sharp, Sequenom, Inc., San
Diego, Germany
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Closing of the Symposium
Lecture hall HS 14
12:30 - 13:00
“Closing of the Symposium.”
Michael W. Pfaffl, H. H. D. Meyer
& Mikael Kubista
13:00 – 14:00 Lunch
in the student cafeteria
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Friday 5 March 2004
Workshop Agenda
12:00 – 14:00
Registration
14:00 – 14:30 Welcome
& Opening of the Application Workshop by Prof. M. Kubista
Lectures by the Workshop participating
Companies:
Lecture hall HS 15
14:30 "The new
LightCycler 2.0: an advanced multi-channel system for rapid
real-time PCR."
Oliver Geulen, Roche Diagnostics,
LightCycler Devolpment Group, Germany
14:50 “Complete
Solutions for Real-Time PCR approaches – Bio-Rad Real-Time
PCR Systems.”
M. Neusser & Luis Ugozzoli, Bio-Rad
Laboratories GmbH, 80939 München, Germany
15:10
"Haplotype Analysis using a Novel Real-Time Amplification
Strategy on the MJ Research
Opticon Continuous Fluorescence Detection System."
Andree Chas, MJ Research Inc., Waltham,
MA, USA
15:30 – 16:00 Coffee
break
16:00 "New tools
for genetic research: Whole genome microarrays and customized
low density solutions"
Thomas Rygus & Thomas Schild,
Applied Biosystems, Germany
16:20
"Multiplexing your assay, from simplex to fourplex."
Fabrice Magnino, Stratagene Europe,
Amsterdam, The Netherlands
16:40
"Optimizing Assays in real time amplification."
Thomas Kaiser, Corbett Research R&D,
Australia sponsered by Pyrosequencing, Sweden
17:00
"Normalization using the F3 Channel of the Lightcycler-
a New Reporter Enables
Multiplexing with 5’Nuclease Probes."
Mary Katherine Johansson, Biosearch
Technologies, Novato, CA, USA
"A Two-Color TaqMan Assay on the LightCycler
1.2."
Brian
E. Caplin, Fluorescentric Inc.
17:20
"The effect of consumable type on the sensitivity and reproducibility
of QPCR."
Sarah Freshwater, ABgene, Blenheim
Road, Epsom, UK
17:40
“Test Systems for Fast and Automated Molecular Diagnostics.”
William A. McMillan, Cepheid
18:00 "BD
QZYME Assay for real-time quantitative PCR."
Bob Larsen, BD BioSciences
18:20
Open evening & visit the Nightlife of Freising
www.freising.de
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Saturay 6 March 2004
qPCR Application Workshop
http://qPCR2004.gene-quantification.info/qpcr2004-workshop.html
Workshop Oral presentations PDFs can
be downloaded in the password protected area.
http://qPCR2004.gene-quantification.info/pub/pub-main.html#ws
Workshop agenda and organization
The 80 participants will be divided into two groups of 40 persons
in each. One group will have seminars covering practical
aspects of qPCR and upstream processes, before lunch and hands-on experiments
and data-analyses after lunch.
The other group will have hands-on before lunch and seminars
after.
Seminars:
Part 1: Introduction
Part 2: Quantification
Strategies
Covering Absolute, Relative and Comparative Quantification.
How standard curves are established and the effects of efficiency
estimations for quantification results.
Several examples are presented that demonstrate the effects
of erroneous efficiency estimations.
Part 3: Nucleic
acids extraction
The most common extraction methods of nucleic acids and their pros
and cons.
Quality control of nucleic acids for qPCR.
Part 4: Reverse
Transcription
The available methods and strategies for reverse transcription
as well as practical considerations when performing RT.
Introduction
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Part 1 of
4
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Part 2 of
4
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Part 3 of
4
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Part 4 of
4
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Hands-on:
Each group of 40 persons is divided so that a small group
of approximately 4 persons is stationed at each instrument.
This group sets up, programs and starts an experiment on that
machine.
The groups will the rotate and get to see experimental data
on the next station/instrument and see how software, setup and analysis
works on that instrument. Also another type of experiment is performed
on each instrument.
The groups rotate until they have seen all experiment/instrument
types.
One instructor
will be available for each station as well as company representatives
to help assist the programming of the software and answer any questions.
The instruments and experiments:
LightCycler - SNP detection using FRET probes
iCycler - Duplex reaction using Molecular Beacons
myCycler - SYBR Supermix- insitu calibration
Stratagene - BEBO and BOXTO, new dyes for qPCR
Opticon 2 - SYBR Green siRNA knockdown
SmartCycler - SYBR Green
ABI Prism 7900 - TaqMan Assay
Rotorgene
3000 - QZyme (BD Biosciences)
For setup of the experiments the participants
will have approximately 1 hour and then approximately ½ hour on
each platform.
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Poster Presentations
Full PDF Posters can be downloaded
in the password protected area.
http://qPCR2004.gene-quantification.info/pub/pub-main.html#po
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Poster Session:
qPCR Application in Clinical Diagnostics
P001
Real time PCR method to differentiate
between homozygous and hemizygous fish.
Agarwal-Mawal Alka, Mawal Yogesh,
Shears Margaret & Fletcher Garth
amawal@aquabounty.com
Aqua Bounty CanadaTM, St. John's,
NL Canada
P002
Higher expression of RANKL in osteoporosis
than in osteoarthrosis
ARKO B1, BITENC LOGAR D1, KOMADINA
R2, KOCIJANČIČ A3, MARC J1 (janja.marc@ffa.uni-lj.si)
1 Faculty of Pharmacy, University
of Ljubljana, Slovenia
2 Department of Traumatology,
General and Teaching Hospital Celje, Slovenia
3 Department of Endocrinology
and Metabolic Diseases, Clinical Centre, Slovenia
P003
Diagnostics of apple proliferation
(AP) phytoplasma by real-time PCR
BARIC S & DALLA VIA J (sanja.baric@provinz.bz.it)
Research Centre for Agriculture
and Forestry Laimburg, Province of Bolzano / Bozen, Italy
P004
A highly sensitive real-time PCR assay for
the detection of HCV RNA in human cells
BEN-PORATH J1, ORDENTLICH A2,
SERY T1, AVIEL S1, SLAMA D1, MISHORI E1, NUSSBAUM O1,
ZAUBERMAN A1 AND DAGAN S1. (jben-porath@xtlbio.com)
1 XTL Biopharmaceuticals Ltd.,
Rehovot, Israel
2 Dept. of Biochemistry and Molecular
Genetics, Israel Institute for Biological Research,
Ness-Ziona, 74100, Israel.
P005
Sensitive and rapid quantitative
detection of HCV RNA by real-time PCR
BOGOSLOVSKAYA E, TSYGANOVA G,
ZAITSEV V, SHIPULIN G (lenbo@pcr.ru)
The central research institute
for epidemiology, Russian Federation
P006
Identification of topical anti-ageing
compound using gene expression studies on Human reconstituted
epidermis.
Bonnet-Duquennoy, M.; Lazou, K.
; Benlahcem, F.; Noblesse, E.; Schnebert, S.;
Kurfürst, R. and Mahé, C.. (mbonnetduquennoy@diormail.com)
Laboratoires R&D – LVMH Recherche
, Saint Jean-de-Braye – 45804, France.
P007
Development of a commercial available real-time
PCR assay for the detection of Hepatitis-A virus: fields
of application
HEITMANN A1, LAUE T1, SCHOTTSTEDT
V2, DOTZAUER A3, PICHL L2 (heitmann@artus-biotech.de)
1 artus GmbH, Hamburg, Germany
2 DRK Blutspendedienst West, Hagen,
Germany
3 Department of Virology, University
of Bremen, Bremen, Germany
P008
A RQ-PCR APPROACH FOR QUANTITATION
OF GLIVEC RESISTANCE ASSOCIATED MUTATIONS.
GRUBER F.X.E.1, 2LAMARK T., 1OLSEN
M., 1HOKLAND K., 1SKOGEN B., 1Dept of Imunology and Transfusion
Medicine, University Hospital of Northern Norway
2Dept of Medical Biology, University
of Northern Norway, Tromsoe, Norway.
(Franz.Gruber@unn.no)
P009
Identification and quantification of human
polyomavirus BK (BKV) in renal allograft patients
Hilmarsen HT1, Bjørang
O1, Midtvedt K2, Scott H1, Nymoen DA1, Silye A1 and Andresen
PA1.
(hilde.tveitan@rikshospitalet.no)
1 Department of pathology and
2Department of Nephrology, National University Hospital,
Oslo, Norway
P010
Multiplex real-time PCR assay
for detection of HCV and HIV RNA and HBV DNA in blood
donations
BOGOSLOVSKAYA E, TSYGANOVA G,
SHIPULIN G (lenbo@pcr.ru)
The central research institute
for epidemiology, Russian Federation
P011
Application of laser microdissection
and real-time Q-PCR on immunocytochemically identified
neurons of the human brain: results from post-mortem material.
KONTOSTAVLAKI D.1,2,3, SLUIJS
J.A.1, UNMEHOPA U.1, SWAAB D.F.1, PANAYOTACOPOULOU M.T.2,3,
HUITINGA I.1 and HOL E.M.1 (mpana@cc.uoa.gr)
Graduate School for Neurosciences,
Netherlands Institute for Brain Research, Amsterdam
Department of Psychiatry,
University of Athens and
University Mental Health Research
Institute, Athens, Greece
P012
Detection and quantitation of
residual DNA in biopharmaceuticals by real – time PCR
technique
Kovač M1, Toplak N1, A. Plaper2
(omega@omega.si)
1 Omega d.o.o., Dolinškova 8,
1000 Ljubljana
2 KRKA d.d , Šmarješka cesta 6,
7000 Novo Mesto
P013
Characterization of mtDNA SNP
typing using quantitative real-time PCR for forensic
purposes with special emphasis on heteroplasmy detection and
mixture ratio assessment
NIEDERSTÄTTER H1, COBLE MD2,
PARSONS TJ2 & W PARSON1 (walther.parson@uibk.ac.at)
1 Institute of Legal Medicine,
University of Innsbruck, Müllerstrasse 44, 6020 Innsbruck
2 Armed Forces DNA Identification
Laboratory, Armed Forces Institute of Pathology, Rockville,
MD, USA
P014
QUANTITATIVE REAL-TIME RT-PCR
ANALYSIS OF PSA AND HK2 MRNA IN CIRCULATIG CELLS FROM
PATIENTS WITH PROSTATIC DISORDER
RISSANEN nèe MÄKINEN
M 1, NURMI J1 , KORPIMÄKI T1, NURMI2 M, PETTERSSON
K1 (majoma@utu.fi)
1 Department of Biotechnology,
University of Turku, Finland
2 Department of Surgery, University
of Turku, Finland
P015
Rapid and highly sensitive pharmacogenetic
diagnostics using real-time PCR
Eckart Schnakenberg, artus GmbH,
Germany
P016
Biological reference materials
for BCR-ABL RNA detection by RQ-PCR assays for therapeutic
monitoring and optimization
SILVY M.1, HEATH A.2, SALDANHA
J.2 and GABERT J1.* (jgabert@ap-hm.fr)
1 Bioch. & Mol. Biol. Lab.ERT
MEIDIA, APHM -IFR Jean roche, MARSEILLE, France ; 2 National
Institute of Biological Standards and Controls, South Mimms,
United Kingdom; * for the international consortium on RQ-PCR standards
P017
Real-time quantification of gene
transcription in the causative agents of Dutch-elm disease
TADESSE YOHANNES1, BERNIER LOUIS2,
E HINTZ WILLIAM3, H HORGEN PAUL1
(ytadesse@utm.utoronto.ca)
1 Department of Botany, University
of Toronto at Mississauga, Mississauga, Ontario, Canada
2 Centre de Recherche en Biologie
Forestière, Université Laval, Quebec,
Canada
3 Department of Biology, University
of Victoria, British Columbia, Canada
P018
Evaluation of a real-time PCR
for the diagnosis of adenoviral keratoconjunctivitis
in clinical eye-swabs
TOLLMANN F, SCHUBERT J, WEISSBRICH
B (tollmann@vim.uni-wuerzburg.de)
Institute of Virology and Immunology,
University of Würzburg, Germany
P019
Real time quantitative PCR machines
in onco-hematology: a cost effectiveness analysis
SILVY M.1, MENOT GENRE C.²,
MANCINI J.3, THIRION X.3, MOATTI JP.² and GABERT J.1
(jgabert@ap-hm.fr)
1Laboratoire de Biochimie et Biologie
Moléculaire, Hôpital Nord, Bd P. Dramard,
Marseille, France
²INSERM U379, Institut Paoli
Calmettes, Marseille, France
3DIM AP-HM, Hôpital Sainte
Marguerite, Marseille, France
P020
Quantitative real-time PCR using
sequence-specific primers to discriminate between donor
and recipient specific mitochondrial DNA polymorphisms
WARNER JB1, HANNIG H2, BRUIN-VAN
DIJK EJ1, VAN DER STEEGE G1, DE LEIJ LFMH1 & HSP GARRITSEN3
(h.garritsen@klinikum-braunschweig.de)
1 Department of Medical Biology,
PAT-LABG, University of Groningen, The Netherlands
2 Centre for Molecular Diagnostics,
Städtisches Klinikum Braunschweig, Germany
3 Department of Transfusion Medicine,
Städtisches Klinikum Braunschweig, Germany
P021
A two-reaction real-time PCR assay
covering the entire spectrum of human adenoviruses for
early identification of patients at high risk of disseminated
disease
WATZINGER FRANZ, SUDA MAGDALENA,
EBNER KARIN and LION THOMAS
(franz.watzinger@ccri.at)
Div. Mol. Microbiology and Development
of Genetic Diagnostics, Children´s Cancer Research
Institute, A-1090 Vienna.
P022
Development and analysis of a multiplex
RT-PCR assay for combined expression screening of melanoma
drug resistance target genes
Wittig R1, Salowsky R2, Maa JS3,
Su MJ3, Blaich S1, Mollenhauer J1, Poustka A1, Mueller
O2
ruediger_salowsky@agilent.com
1 Department of Molecular Genome
Analysis, German Cancer Research Center, Heidelberg, Germany;
2Agilent Technologies, Waldbronn, Germany; 3 Maxim Biotech,
San Francisco, USA
P023
Systematic Multiplex PCR and RT-PCR
analyses of changes in copy number and expression of proto-oncogenes
and tumor suppressor genes in cancer tissues and cell lines
YAMAMOTO M1, METOKI R2, &
YAMAMOTO F1 (fyamamoto@burnham.org)
1 Cancer Genetics and Epigenetics
Program, The Burnham Institute, USA.
2 Sendai Yanagyu Urology Clinic,
Japan.
P024
Simultaneous determination of
fetal trisomies 18 and 21 by real-time quantitative PCR
Bernhard Zimmermann, Lisa Levett*,
Wolfgang Holzgreve, Sinuhe Hahn (tvk2000@hotmail.com)
Laboratory for Prenatal Medicine,
University Women’s Hospital, Basel, Switzerland.
* Cytogenetic DNA Services Ltd.,
London, UK.
P025
Identification and quantitative
measurement of BCR/ABL transcripts using quantitative
real-time PCR (Q-PCR)
Silye, A., Hilmarsen, H.T, Nymoen,
D., Beiske, K. and Andresen, P.A.
aleksandra.silye@rikshospitalet.no
Department of Pathology, The National
Hospital, Norway.
P026
Optimised amplification of multicopy
Y-chromosome sequences for the improved quantitative measurement
of fetal male DNA in plasma by real-time PCR
Bernhard Zimmermann, Wolfgang
Holzgreve, Sinuhe Hahn (tvk2000@hotmail.com)
Laboratory for Prenatal Medicine,
University Women’s Hospital, Basel, Switzerland.
P137
Exogenous and endogenous controls for qualitative and quantitative
detection of seminal cell-associated porcine reproductive and respiratory
syndrome virus (PRRSV) RNA
Sandra Revilla-Fernández1,2,
Barbara Wallner1, Friedrich Schmoll3, Elisabeth Hofmann1, Hanno Schreiber1,
Mathias Müller1, Ralf Steinborn1,4
Ralf.Steinborn@vu-wien.ac.at
1Institute of Animal Breeding and Genetics and 3II. Medical
Clinic for Ruminants and Swine, University of Veterinary Medicine,
Veterinärplatz 1, A-1210 Vienna, Austria; and 4Ludwig-Boltzmann
Institute for Immuno-, Cyto- and Molecular Genetic Research, Veterinärplatz
1, A-1210 Vienna, Austria
|
Poster Session: qPCR
Application in Microbiology & Virology
P027
Development of real – time PCR
assay for Chrysanthemum stem necrosis virus
BOBEN, J.1, ZUPANČIČ, M.1, MAVRIČ,
I.1, 2, VOZELJ, N.1, PETROVIČ, N.1, RAVNIKAR, M.1 (jana.boben@nib.si)
1National institute of Biology,
Department of Plant Physiology and Biotechnology, Večna
pot 111, SI-1000,
Ljubljana, Slovenia
2Current address: Agricultural
Institute of Slovenia, Haquetova 17, SI-1000, Ljubljana,
Slovenia
P028
Automated system for isolation
of bacterial DNA
FOSSHEIM T, HARDERSEN H, and HAALAND
IE
(ingerlise.evans@qiagen.com)
QIAGEN AS, Norway
P029
Preliminary results on detection
of Plum pox potyvirus using real-time PCR
MAVRIČ Irena1, TOPLAK Nataša2,
VIRŠČEK MARN Mojca1 (irena.mavric@kis.si)
1 Agricultural Institute of Slovenia,
Hacquetova 17, SI-1000 Ljubljana, Slovenia
2 Omega d.o.o., Dolinškova 8,
SI-1000 Ljubljana, Slovenia
P030
Nucleic acid sequence based amplification
(NASBA) Chlamydia trachomatis 16S-rRNA with Real-time
detection
GUSCHIN A.E., RYZHIKH P.G., SHIPULIN
G.A. (aguschin@pcr.ru)
Central Research Institute for
Epidemiology, Moscow, Russian Federation
P031
Detection of Mycobacterium avium
subsp. paratuberculosis in bovine semen by real-time PCR
HERTHNEK D1, ENGLUND S1, WILLEMSEN
PTJ2, BÖLSKE G1 (david.herthnek@sva.se)
1 National Veterinary Institute
(SVA), Uppsala, Sweden
2 CIDC-Lelystad, Central Institute
for Animal Disease Control, Lelystad, Netherlands
P032
A Validated In-House PCR Method for the
Detection of Chlamydia trachomatis from Urine and Swabs
Using Automated Sample Preparation.
HJELMEVOLL S. O1, OLSEN M. E1,
HAAHEIM H1 (stig.ove.hjelmevol@unn.no)
1 Department of Medical Microbiology,
University Hospital of North Norway, Norway
P033
Implications of Molecular Evolution
on Viral Quantification using real-time PCR based assays
KLEIN D, BRUNNER B & STEINRIGL
A
(dieter.klein@vu-wien.ac.at)
Institute of Virology, University
of Veterinary Medicine Vienna, Austria
P035
Real-time and End-point PCR detection
of T. pallidum DNA and RNA for clinical and research
application.
GUSCHIN A.E., RODIONOVA E.N.,
SHIPULIN G.A. (aguschin@pcr.ru)
Central Research Institute for
Epidemiology, Moscow, Russian Federation
P036
Development and validation of
a real time PCR to quantify Parvovirus B19-DNA in plasma
pool. A normative approach.
G. Nardi1, W.K. Roth2, A. Morelli1,
A. Falbo1, C. Nardini1, E. Moretti1
(g.nardi@kedrion.com)
1 Process and Analytical Development,
Kedrion S.p.A., Italy
2 Institute of Transfusion Medicine
and Immunohaematology Blood Transfusion Centre of the
German Red Cross, Frankfurt, Germany
P037
Differentiation for viable and
heat-inactivated Salmonella Enteritidis by LightCycler
real-time PCR
NOTZON A, BAUER J (Angelika.Notzon@wzw.tum.de)
Lehrstuhl für Tierhygiene,
Technische Universität München-Weihenstephan,
Germany
P038
Real-time and end-point PCR assay
for detection of Neisseria gonorrhoeae in clinical specimens
using dual-labeled fluorescent probes.
SAVOCHKINA YA, GUSCHIN AE, CHICHKANOVA
YV, SHIPULIN GA (savochkina@pcr.ru)
Central Research Institute for
Epidemiology, Moscow, Russian Federation
P039
Quantification of HCMV DNA in
whole blood by Real-Time RotorGene-Based PCR. Comparison
of two approaches of normalization.
SHIPULINA O, SLUGINA T, SHIPULIN
G. (olga.shipulina@pcr.ru)
Central Research Institute for
Epidemiology, Moscow, Russia.
P040
Quantification of MADS box genes
transcription in the orchid Dendrobium thyrsiflorum
Skipper M1 & Pedersen KB2
(martins@bot.ku.dk)
1Botanical Institute, University
of Copenhagen, Gothersgade 140, DK-1123 Copenhagen K,
Denmark. 2MJ Research Representative Office, Literbuen 10B,
DK-2740 Skovlunde, Denmark.
P041
Switching between metabolic states
in Saccharomyces cerevisiae – a gene expression profiling
study
Ståhlberg A1,2 Otterstedt
K1 Gustafsson L1, Andrade Garda JM3 & Kubista M1,2
(anders.stalberg@molbiotech.chalmers.se)
1 Department of Chemistry and
Biosciences, Chalmers University of Technology, 405 30
Gothenburg, SWEDEN
2 TATAA Biocenter, 405 30 Gothenburg,
SWEDEN
3 Department of Analytical Chemistry,
University of A Coruna, Spain
P042
Evaluation of a confirmatory PCR
assay for Neisseria gonorrhoeae
WHILEY D1, BUDA P2, SYRMIS M1,
BAYLISS J3, COVER L3, BATES J3, & T SLOOTS1,2 (d.whiley@mailbox.uq.edu.au)
1 Clinical Virology Research Unit,
Sir Albert Sakzewski Virus Research Centre, Royal Children’s
Hospital, Queensland, Australia
2 Queensland Health Pathology
Service, Royal Brisbane Hospital, Queensland, Australia
3 Queensland Health Scientific
Services, Queensland, Australia
P045
Quantification of the expression
of the chitinolytic enzyme encoding genes ech30, ech42
and nag1 in Trichoderma atroviride P1 under varying growth conditions
using a real-time RT-PCR assay
Clarke JL1, Tronsmo A2, Clarke
N3 and Klemsdal SS1 (jihong.liu-clarke@planteforsk.no)
1) Plant Protection Center, The
Norwegian Crop Research Institute, Høgskoleveien
7, N-1432 Ås, Norway
2) Department of Chemistry and
Biotechnology, Agricultural University of Norway, N-1432
Ås, Norway
3) The Norwegian Forest Research
Institute, Høgskoleveien 12, N-1432 Ås, Norway
P065
A real-time RT-PCR SYBR Green-I
assay for detection of porcine reproductive and respiratory
syndrome virus
Hjulsager CK1, Jørgensen
PH2, Larsen LE1, Storgaard T3 & Bøtner A4 (ckh@dfvf.dk)
1 Department of Veterinary Diagnostics
and Research, Danish Institute for Food and Veterinary
Research, Denmark
2 Department of Poultry, Fish
and Fur Animals, Danish Institute for Food and Veterinary
Research, Denmark
3 Novo Nordisk A/S, Applied Trinomics,
Denmark
4 Department of Virology, Danish
Institute for Food and Veterinary Research, Denmark
P132
Real-time PCR Detection of Orthopoxvirus
DNA and Identification of Variola Virus DNA by Melting
Analysis
NITSCHE ANDREAS, ELLERBROK HEINZ
AND PAULI, GEORG (nitschea@rki.de)
Robert Koch-Institut, Zentrum für Biologische Sicherheit
1, Nordufer 20, D-13353 Berlin
P133
Absolute quantification of pathogens in food
samples using floatation as a novel sample treatment method
prior to qPCR.
WOLFFS P. and RÅDSTRÖM P. (petra.wolffs@tmb.lth.se)
Applied Microbiology, Lund Institute
of Technology, Lund University, Sweden
|
Poster Session:
Normalization & Standardization &
Quality control
P043
Design and testing of glyceraldehyde-3-phosphate
dehydrogenase (GAPD) primers to avoid pseudogenes amplification
in Real-Time PCR.
CARRARO G , ALBERTIN G , NUSSDORFER
GG (gianni.carraro@unipd.it)
Department of Human Anatomy and
Physiology, University of Padua, Italy
P044
Development of a Quantitative
Real-Time Polymerase Chain Reaction (QRT-PCR) Assay using
Hybridisation Probes to Detect the Fungus Aspergillus
CHONG WY1, NG WL2, KOAY ESC2,3
(patkoaye@nus.edu.sg)
1Department of Biological Science,
Faculty of Science, National University of Singapore
2Molecular Diagnosis Centre, Department
of Laboratory Medicine, National University Hospital,
Singapore
3Department of Pathology, Faculty
of Medicine, National University of Singapore
P046
Stability of six housekeeping
genes in nine ovine tissues and its application to the
relative quantification of prion protein gene expression
GARCIA-CRESPO D, JUSTE RA &
HURTADO A (dgarcia@neiker.net)
Department of Animal Health, Basque
Institute for Agricultural Research and Development (NEIKER),
Spain
P047
Analysis of the expression stability
of Actinobacillus pleuropneumoniae housekeeping genes during
in vitro growth under iron-depleted conditions by Real-Time
quantitative PCR
Nielsen, K. K1 & Boye, M1
(kni@dfvf.dk)
1 Department of Veterinary Diagnostics
and Research, Danish Institute for Food and Veterinary
Research, Copenhagen, Denmark
P048
New tools for quality control in real-time
PCR experiments
Salowsky R1, Lightfoot S2, Schroeder
A1, Mueller O1 (ruediger_salowsky@agilent.com)
1 Agilent Technologies, Waldbronn,
Germany; 2 Agilent Technologies, Palo Alto, USA
P049
Search by cluster analysis for
steadily expressed genes with application as normalisation
index in real-time RT-PCR.
Tichopád1 Aleš & Michael
W. Pfaffl2 (pfaffl@wzw.tum.de)
1 IMFORM GmBH, International Clinical
Research, Birkenweg 14, Darmstadt, Germany
2 Physiology – Weihenstephan,
Freising, Germany
P050
Comparative Quality Assessment
(CoQA) for real-time PCR
BAR T1, MUSZTA A2 , ANDRADE-GARDA
J.M.3 & KUBISTA M1,4 (tzachi.bar@molbiotech.chalmers.se)
1Department of Chemistry and Bioscience
Chalmers University of Technology Medicinargatan 7B 405
30, Gothenburg, Sweden
2Department of Mathematical statistics,
Chalmers University of Technology Eklandagatan 86, 412
96, Gothenburg, Sweden
3Department of Analytical Chemistry,
University of A Coruna, A Zapateira s/n E-15071 A Coruna,
Spain
4TATAA Biocenter, Medicinargatan
7B 405 30, Gothenburg, S
P051
Real-time PCR survey
ADAMS PS1, BAO Y2, GROVE DS3,
HOLLOWAY B4, SHIPLEY GL5, YEUNG AT6 (dsg4@psu.edu)
1Trudeau Institute, Saranac Lake,
NY, United States
2University of Virginia School
of Medicine, Charlottesville, VA, United States 3Pennsylvania
State University, University Park, PA, United States
4CDC, Atlanta, GA, United States,
5University of Texas Health Science
Center, Houston, TX, United States,
6Fox Chase Cancer Center, Philadelphia,
PA, United States.
P052
Amplitude normalization in real
time PCR data processing
Larionov A.L., Hulme M.J., Miller
W.R.
( alexey@larionov.co.uk )
Breast Unit Research Group, Western
General Hospital, Edinburgh, UK
P053
Performance of different qPCR
approaches: Comparing accuracy and reproducibility of
conventional 5’ nuclease probes, short LNA enhanced probes
and SYBR Green
NIELSEN PS1, FINK T2, RAMSING
NB1 & MOURITZEN P1 (mouritzen@exiqon.com)
1 Exiqon A/S, Vedbaek, Denmark
2 Department of Health Science
and Technology, Aalborg University Stem Cell Research,
Denmark
P054
The influence of pigmentation and evaporation on Quantitative
PCR
Van der Valk A M1, 2, O’Shaughnessy M C1, Baker S C1,3,
Ng S1, Sarker D K2 & Lloyd A W2 (mego@abgene.com)
1 ABgene, ABgene House, Blenheim Road, Epsom, Surrey,
KT19 9AP, UK; 2 School of Pharmacy and Biomolecular Sciences,
University of Brighton, Moulsecoomb, Brighton, BN2 4GJ, UK;
3 School of Biological and Chemical Sciences, Birkbeck University of
London, Malet Street, Bloomsbury, London, WC1E 7HX, UK
P055
Integration of Comparative Quality
Assessment (CoQA) for real-time PCR into Kinetic Outlier
Detection (KOD)
BAR T1, SVANBERG B2, EDLING M2,
WATERS S2, MUSZTA A3 & KUBISTA M1,4
(tzachi.bar@molbiotech.chalmers.se)
1Department of Chemistry and Bioscience,
Chalmers University of Technology, Medicinargatan 7B 405
30, Gothenburg, Sweden
2Carlsson Research AB, Medicinargatan
11, 405 30, Gothenburg, Sweden
3Department of Mathematical statistics,
Eklandagatan 86, 412 96, Gothenburg, Sweden
4TATAA Biocenter, Medicinargatan
7B 405 30, Gothenburg, Sweden
|
Poster Session:
Transcriptomics & Expression profiling
P056
qRT-PCR as a tool for studying
the expression pattern of Wnt genes, their receptors and
their secreted antagonists in the Mouse Embryo
ABDO S1, WILLEMS E1, KEMP C1 &
LEYNS L1 (lleyns@vub.ac.be)
1 Laboratory for Cell Genetics,
Free University of Brussels, Pleinlaan 2, 1050 Brussels,
Belgium
P057
Telomerase reverse transcriptase
expression in twenty-three acute promyelocytic leukemia
patients: relative quantification by real-time polymerase chain
reaction.
Calatroni S1, Klersy C2, Rocca
B1, Boni M1, Cavigliano PM1, Giardini I1, Zappatore R1,
Corsetto N1, Caresana M1, Bernasconi C1, Bernasconi P1 (p.bernasconi@smatteo.pv.it)
1 Division of Hematology, IRCCS
Policlinico San Matteo, University of Pavia
2 Scientific Direction, Clinical
Epidemiology and Biometry Unit, IRCCS Policlinico San
Matteo, Pavia, Italy
P058
RGS2 expression and intracellular
calcium mobilization in fibroblasts from hypertensive
patients
CEOLOTTO G., BARITONO E., LENZINI
E., ORSO E., SARTORI M., CICCARIELLO L., PAPPARELLA
I., SEMPLICINI A. (giulio.ceolotto@unipd.it)
Department of Clinical and Experimental
Medicine, University of Padova Medical School, Padova,
Italy
P059
Use quantitative real-time RT-PCR
to measure innate immunity
Chang J-S, Huggett J, Kim L, Dheda
K, Zumla A, Rook G (j.chang@ucl.ac.uk)
Centre for Infectious Diseases
and International Health, UCL and Royal Free Medical
School, London
P060
Evaluation of HR-HPV-E6-E7 mRNA
in histologically negative sentinel lymph nodes of cervical
carcinoma patients as a predictive marker for recurrence.
Dürst M, Altgassen C, Müller
B, Greinke C, Haefner N and Schneider A for the AGO “Uterus
3” study group (matthias.duerst@med.uni-jena.de)
Klinik für Frauenheilkunde
und Geburtshilfe der FSU Jena, Abt. Frauenheilkunde,
Bachstraße 18, D-07743 Jena
P061
The in vivo effects of a Mycophenolic
Acid (MPA) treatment on the cytokine expression in sheep
leucocytes, using an efficiency corrected relative quantification
model in real-time RT-PCR.
Dzidic A, Meyer HHD, Pfaffl MW
(pfaffl@wzw.tum.de)
Institute of Physiology, Center
of Life and Food Science, TUM– Weihenstephan, Freising,
Germany
P062
Analysis of differentially expressed
genes in kidneys of PEPT2 knockout mice
FREY I1, SAILER D1,RUBIO-ALIAGA
I2, DROBYSHEV A2, BECKERS J2, DANIEL H1
(frey@wzw.tum.de)
1, Molecular Nutrition Unit, WZW
Weihenstephan, Technical University of Munich, Germany
2, Institute of Experimental Genetics,
GSF, Neuherberg, Germany
P063
Norepinephrine transporter knockout-mediated
regulation of adrenergic receptor mRNAs in mice brain
1Gilsbach Ralf, 1Loebbe Sandra,
1Brüss Michael, 2Caron Marc, 1Bönisch Heinz
(boenisch@uni-bonn.de)
1 Institute of Pharmacology &
Toxicology, University of Bonn (Germany)
2 Duke University, Med. Center,
Durham, NC (USA)
P064
Application of real-time PCR for
quantitative determination of transgene copy number in
transformed CHO cell lines
GRUDEN K., HREN M., POMPE-NOVAK
M., RAVNIKAR M. (matjaz.hren@nib.si)
Department of Plant Physiology
and Biotechnology, National Institute of Biology, Večna
pot 111, Ljubljana Slovenia
P066
Development of real-time RT-PCR
assays for relative gene expression estimation of equine
cytokines
KNAPP E, ONMAZ AC, VAN DEN HOVEN
R, KLEIN D (elzbieta.knapp@vu-wien.ac.at)
Institute of Virology, University
of Veterinary Medicine Vienna, Austria
P067
Modular concept of a real time
PCR-assay for determination of the quantity and quality
of mitochondrial and nuclear DNA
KÖCHL S, NIEDERSTÄTTER
H, PARSON W ( walther.parson@uibk.ac.at )
Institute of Legal Medicine, University
of Innsbruck, Austria
P068
The Profiling of Estrogen-Response
Elements in Estrogen Target Genes of MCF7 Breast Tumor Cells
Kong SAY LI1, Lin CHIN-YO1, Edison
Liu TAK-BUN1 (kongsl@gis.a-star.edu.sg)
1Genome Institute of Singapore,
Singapore
P069
USE OF REAL-TIME RT-PCR TO COMPARRE
GENE EXPRESSION IN MALE AND FEMALE Brugia malayi| ADULT
WORMS
Li BW, Rush A, Weil, GJ. (bli@im.wustl.edu)
Washington University School of
Medicine, Department of internal Medicine, St Louis,
MO, USA
P070
Beneficial applications of cell
line and primary cell Q-PCR data.
MURDOCK PR,1 WALHIN JP1, STRUM
JC2, STEPLEWSKI KM3, CARRICK KM2, MAURIO FP2, WILSON JM1,
EVERETT DM1, KELLY FM1, MARTENSEN SA2, BERG S2 and JP COGSWELL2
(Paul.R.Murdock@gsk.com)
1 GlaxoSmithKline, Medicines Research
Centre, Stevenage, UK
2 GlaxoSmithKline, Five Moore
Drive, Research Triangle Park, North Carolina, USA.
3 GlaxoSmithKline, Upper Providence,
Philadelphia, PA, USA.
P071
Innate Immune Receptors of the
TLR Family in Human Skin Disease: Profiling of Functional
Gene Expression in Epidermal Keratinocytes
NADERI-KALALI B,† KÖLLISCH
G,†* OLLERT M,†§ (naderi@gsf.de)
†Department of Dermatology and
Allergy, Biederstein,
§Clinical Research Division
of Molecular and Clinical Allergotoxicology
*Division of Environmental Dermatology
and Allergy GSF/TUM, GSF National Research Center for
Environment and Health, Neuherberg, Germany
P072
Quantitative mRNA expression of
the IGF-system members during induced luteolysis in the
bovine.
TP Neuvians, MW Pfaffl, D Schams,
B Berisha (tanja.neuvians@path.ma.uni-heidelberg.de)
Physiology-Weihenstephan, Technical
University of Munich, Freising, Germany
P073
Abundance of mRNA coding for components
of the somatotropic axis in different layers of the jejunum
and ileum of neonatal calves
ONTSOUKA E.C., PHILIPONA C., HAMMON
H.M. & BLUM J. W.
(juerg.blum@itz.unibe.ch)
Division of Nutrition and Physiology,
Institute of Animal Genetics, Nutrition and Housing,
Faculty of Veterinary Medicine, University of Berne, Switzerland
P074
Investigations of a unique line
of iron-resistant rat cardiac myoblasts using quantitative
RT-PCR
QIAN M, GAO X & JW EATON (mwqian2@yahoo.com)
Molecular Targets Group, James
Graham Brown Cancer Center, University of Louisville, KY
40202, USA
P075
Analysis of Substance P receptor
mRNA in periosteum and capsular tissue of adjuvant arthritic
rat using hemi-nested RT-PCR
QURESHI AYAZ A1. , MOATTER TARIQ2
, HASHMI PERVAIZ M.1 , AHMED MAHMOOD3, (ayaz.alam@aku.edu)
Department of Surgery, Aga Khan
University Hospital Karachi, Pakistan.
Department of Pathology and Microbiology,
Aga Khan University Hospital Karachi, Pakistan.
Department of Orthopaedics, Karolinska
Hospital, Stockholm, Sweden.
P077
Inducible and endothelial nitric
oxide synthases (NOS) mRNA in the bovine ovary
Ulbrich SE1, Berisha B1, Schoenfelder
M2, Welter H1, Schams D1 (Ulbrich@wzw.tum.de)
1 Physiology-Weihenstephan, Technical
University of Munich, Freising, Germany
2 Institute of Public Health Research,
Technical University of Munich, Munich, Germany
P078
Expression of E coli gene transcript
at various stages of growth, under the control of stationary
phase promoters
VIKRAM H1, FRIEHS K2, MIKSCH K2,
FLASCHEL E2
hvi@fermtech.techfak.uni-bielefeld.de
1 International Graduate
School in Bioinformatics and Genome Research, Universitaet
Bielefeld, Universitaetstr 25, D-33615, Germany
2 Lehrstuhl für Fermentationstechnik,
Technische Fakultät, Universitaet Bielefeld, Universitaetstr
25, D-33615, Bielefeld, Germany
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Poster Session:
Food Hygiene & GMO
& Agrobiotechnology
P076
Determining the expression level
of transgene in transformed plants – Method of choice?
TOPLAK N 1, 2, OKRŠLAR V1, STANIČ-RACMAN
D1, ŽEL J1, GRUDEN K1, 3 (natasa.toplak@nib.si)
1 National Institute of Biology,
Department of Plant Physiology and Biotechnology, Večna
pot 111, 1000 Ljubljana, Slovenia
2 Omega d.o.o., Dolinškova
8, 1000 Ljubljana, Slovenia
3 Institute Jožef Štefan, Jamova
39, 1000 Ljubljana, Slovenia
P079
Real-time PCR: accuracy of GMO
quantification
DREO T1, CANKAR K1, ŠTEBIH D1,
GRUDEN K1, RAVNIKAR M1, ŽEL J1 (tanja.dreo@nib.si)
1National Institute of Biology,
Department of Plant Physiology and Biotechnology, Večna
pot 111, 1000 Ljubljana, Slovenia
P080
Real time RT-PCR as a tool to
study differential gene expression in the potato-late
blight interaction
GIGLIOTTI S1, NICOT N1, SOLINHAC
L1, GHISLAIN M2, HAUSMAN JF1 & D EVERS1 (gigliott@crpgl.lu)
1 CRP-Gabriel Lippmann, CREBS
research unit, Luxembourg
2 International Potato Center,
Department of Crop Improvement and Genetic Resources, Lima,
Peru
P081
Applications for qPCR in applied
plant biotechnology
KAMNERT, IRÉNE (Irene.Kamnert@plantscience.se)
BASF Plant Science, Plant Science
Sweden AB, Herman Ehles vag 3-4, SE-268 31 SVALÖV,
SWEDEN
P082
Real-Time PCR Systems for Salmonella
spp. and Listeria monocytogenes Detection in Food Products
M. Malchow1, V. Schindler2, H.
Fouché2, N. Le Bellec2, F. Préaudat2, J.-P.
Tourniaire2, & M. Le Guern-Fellous2,
1 Bio-Rad Laboratories GmbH, Munich,
Germany;
2 Bio-Rad Laboratories, Marnes
La Coquette, France
P083
Quantitative PCR in a high-throughput
agricultural biotechnology setting
MEYER S, HONDRED D, LENDERTS B,
LEYSENS N, PENNINGTON-COPE N, PETERSBURG T, PIETILA J,
THOMPSON L (sandra.meyer@pioneer.com)
Analytical and Genomics Technologies,
Pioneer Hi-Bred, International, a DuPont Company, USA
P084
SOD, CAT and GSH-Px mRNA expression
in liver of Atlantic salmon Salmo salar exposed to hyperoxic
conditions during smoltification
Olsvik, Pål A. (pal.olsvik@nifes.no)
National Institute of Nutrition
and Seafood Research, Bergen, Norway
P085
The use of Real-Time PCR for GMO
detection: Comparison of commercial and in-house protocols.
OVESNÁ J., KUČERA L., CHÁB
D., JELÍNKOVÁ E., MACHALOVÁ V. (ovesna@vurv.cz)
Department of Molecular Biology,
Research Institute of Crop Production, Prague 6 – Ruzyně,
161 06, Czech Republic
P086
PCR Amplification: Is it simple
for Wildlife Forensic Samples?
Reeta Sharma and S.P. Goyal
(reeta@wii.gov.in)
Wildlife Forensic Cell, Wildlife
Institute of India, P.O. Box 18, G.P.O, Chandrabani, Dehra
Dun 248001, India
P087
Expression studies of lipid metabolism
genes in Atlantic salmon (Salmo salar) – effects of replacing
dietary fish oil with rapeseed oil.
TORSTENSEN, B. E.1), HORDVIK,
I.2), DOUGLAS, S.3), LALL, S. P.3) & JORDAL, A.-E.1)
(bente.torstensen@nifes.no)
1 NIFES (National Institute of
Nutrition and Seafood Research), Bergen, Norway.
2 Institute of Fisheries and Marine
Biology, University of Bergen, Bergen, Norway.
3 Institute of Marine Biosciences,
NRC, Halifax, Canada.
P088
Development of Real-time PCR kit
for detection and quantification of genetically
modified soybean in food and feeds.
YATSYSHINA S.B.1,2, ASTACHOVA
T.C.1, SHIPULIN G.A.1, OBUKHOV I.L.2,
PANIN A.N.2 (syatsyshina@pcr.ru)
1 Central Research Institute for
Epidemiology, Moscow, Russia
2 The All-Russia State Centre
for Quality and Standardization of animal medicines and
feeds (VGNKI), Russia
P089
Expression of the myosin heavy
chain gene (MyHC) in Atlantic salmon (Salmo salar L.) fed
with graded amounts of solubilised protein
HEVRØY EM1, JORDAL A-E1,
HORDVIK I2,1, HEMRE G-I1 & OLSVIK P1 (ernst.hevroy@nifes.no)
1 National Institute of Nutrition
and Seafood Research (NIFES), PO Box 176, Sentrum, N-5804
Bergen (Norway)
2 University of Bergen, HiB, N-5020
Bergen, (Norway)
P090
A rapid real-time PCR detection
method for specific bacteria.
WIKMAN T1, ANTONEN K1, KORPIMÄKI
T1 & J NURMI1
(tiina.wikman@utu.fi)
1 Department of Biotechnology,
University of Turku, Finland
P135
Real-time Detection and Quantitation of Genetically Modified Soy.
KARUDAPURAM S, Ph.D.1, FAHEY B, Ph.D.2, &
BATEY D, Ph.D.1 (babettef@mjr.com)
1 MJ Research, South San Francisco, CA
2 MJ Research, Inc., Waltham, MA
P136
Ligation-dependent probe amplification for the simultaneous event-specific
detection and relative quantification of DNA from different genetically
modified organisms.
F. Moreano1, A. Ehlert1 U. Busch2 and K.-H. Engel1*
(K.H.Engel@lrz.tu-muenchen.de)
1 TUM, Lehrstuhl für Allgemeine Lebensmitteltechnologie,
Am Forum 2, 85350 Freising-Weihenstephan, Germany
2 Bayerisches Landesamt für Gesundheit und
Lebensmittel-sicherheit LGL, Veterinärstr. 2, 85764 Oberschleißheim,
Germany
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Poster
Session: New Methods & Approaches
P091
Nucleic Acids Research Group (NARG)
Taqman® primer/probe design study
ADAMS PS1, BAO Y2, GROVE DS3,
HOLLOWAY B4, SHIPLEY GL5, YEUNG AT6 (Sadams@northnet.org)
1Trudeau Institute, Saranac Lake,
NY, United States
2University of Virginia School
of Medicine, Charlottesville, VA, United States
3Pennsylvania State University,
University Park, PA, United States
4CDC, Atlanta, GA, United States
5University of Texas Health Science
Center, Houston, TX, United States
6Fox Chase Cancer Center, Philadelphia,
PA, United States.
P092
A new reporter dye for real-time
PCR binding in the minor groove
BENGTSSON M1, KARLSSON J1, WESTMAN
G1, KUBISTA M1
(martin.bengtsson@molbiotech.chalmers.se)
1Department of Chemistry and Bioscience,
Chalmers University of Technology, and the TATAA Biocenter,
Göteborg, Sweden
P093
Relative Gene Expression Studies
using Multiplex Quantitative PCR on the Bio-Rad iCycler
iQ® Real-Time PCR Detection System.
Faye Boeckman, Larissa Tan, Marni
Brisson, Rob Park and Keith, Hamby,
Bio-Rad Laboratories, 2000 Alfred
Nobel Drive, Hercules, CA
P094
Unique formulations for quantitative
PCR and RT-PCR applications
BROWN CR, VON REIN E &
EASTLUND ER (eeastlund@sial.com)
Sigma-Aldrich Biotechnology, 2909
Laclede Ave., St. Louis, MO 63103 USA
P095
Multiplex qPCR LightCycler Analysis
Ebenbichler C., Boell I., Weilke
C. and Ankenbauer W.
Roche Applied Science, Nonnenwald
2, 82377 Penzberg
P096
Comparison of qPCR with FISH-based
enumeration of undefined starter cultures
FRIEDRICH, U., SCHNEIDER, H.,
AND FRANZEN, K. (udo.friedrich@danisco.com)
Research & Development, Danisco
Niebüll GmbH, 25899 Niebüll, Germany
P097
Dynamical simulation of a typical
real-time PCR system for quantitative DNA detection
Geiseler D., Hörtig W.
(geiseler.dietrich@cvuasig.bwl.de)
Chemisches und Veterinäruntersuchungsamt
Sigmaringen (Germany)
P098
RealMaster Probe Kits – A Novel
System for Quantitative PCR with Target-specific Probes
JaNae Grutt, Ryan Westberry, Jessica
Goodrich, Lars-Erik Peters
Eppendorf 5-Prime, Inc., Boulder,
CO 80301, USA
P099
Mass spectrometric gene expression
analysis – combining competitive PCR and MALDI TOF mass
spectrometry.
Christian Jurinke1, Chunming Ding2,
Paul Oeth1, Charles R. Cantor1 cjurinke@sequenom.com
1SEQUENOM, Inc. San Diego, CA
2Center for Advanced Biotechnology
and Bioinformatics Program, Boston University, Boston,
MA
P100
Development of methods for detection
and quantification of mRNA transcripts from single cells
BENGTSSON M1, STÅHLBERG
A1, KUBISTA M1
(martin.bengtsson@molbiotech.chalmers.se)
1Department of Chemistry and Bioscience,
Chalmers University of Technology, and the TATAA Biocenter,
Göteborg, Sweden
P101
The Kinetic and Mathematical Model
of PCR Amplification Experiment
KE Bing-shen, HUANG Xiang-yan,
CHEN Shi-min, CHEN Ying-jian, QI Fa-liang. kbshen@sina.com
Department of Immunology, General
Hospital of Jinan Military Region, Jinan 250031 China
P103
Evaluation of the performance
of LNA and MGB probes in 50-nuclease PCR assays.
Capucine Letertre, Sylvie Perelle,
Francoise Dilasser, Khalil Arar, Patrick Fach,*
p.fach@afssa.fr
Agence Franc¸aise de Se´curite´
Sanitaire des Aliments (AFSSA), Laboratoire d’Etudes
et de Recherches sur Hygiene et la Qualite des Aliments,
Unite: Atelier de Biotechnologie,
1-5, rue de Belfort, 94700 Maisons-Alfort, France.
Proligo, 1 rue Robert et Sonia
Delaunay, 75011 Paris, France
P104
Real-time immuno-PCR
Lind K1, Nilsson O2 & Kubista
M1 (kristina.lind@molbiotech.chalmers.se)
1 Department of Chemistry and
Bioscience, Chalmers University of Technology and TATAA
Biocenter, Sweden.
2 CanAg Diagnostics, Gothenburg,
Sweden.
P105
qPCR data processing: comparison
of relative quantification methods.
Montjovent, MO1, Pioletti, DP1
marc-olivier.montjovent@epfl.ch
1Bone Bioengineering Group, Orthopedic
Research Center, Swiss Federal Institute of Technology
Lausanne, Switzerland
P106
A Novel Method for Molecular Haplotyping
Combining An Improved AS-PCR Technique with Base Specific
Cleavage of Nucleic Acids Analyzed By Mass Spectrometry
Susanne Müller1, Martin Beaulieu2,
Dominik Kosman2, Matthew R. Nelson2, and Dirk van den
Boom2 (smuller@sequenom.de)
1SEQUENOM GmbH, Mendelssohnstrasse
15D, 22761 Hamburg, Germany
2SEQUENOM Inc., 3595 John Hopkins
Court, San Diego, CA 92121, USA
P107
ResonSense®: Simple linear
probes for rapid fluorogenic PCR
Lee, M A, BioGene Ltd, BioGene
House, Harvard Way, Kimbolton, Cambs PE28 ONJ.
m.lee@biogeneresearch.co.uk
P108
Improvement of gene expression
analysis by RQ-PCR technology: addition of BSA.
Silvy M., Pic G., Gabert J. and
Picard C.
(jgabert@ap-hm.fr)
ERT-MEIDIA, IFR Jean Roche, Faculté
de Médecine NORD, Bd Pierre Dramard, 13916 Marseille
Cedex 20, France.
P109
Trehalose is a potent PCR enhancer:
reduction of DNA melting temperature and thermal stabilization
of Taq Polymerase by the disaccharide trehalose.
Spiess AN1, Mueller N1 & Ivell
R1 (spiess@ihf.de)
1Institute for Hormone and Fertility
Research, Falkenried 88, 20251 Hamburg, Germany
P110
High confidence SNP scanning with
the ds DNA dye, LCGreen I, in conjunction with high resolution
melting analysis.
Reed G1, Pryor R1, Wittwer C1.
(g.reed@med.utah.edu)
1 Department of Pathology, University
of Utah, USA
P111
Rapid real-time PCR using a SuperConvectionä
instrument.
SVANVIK N1, MALMQVIST MATS1 (nicke.svanvik@alphahelix.com)
1. AlphaHelix Molecular Diagnostics
AB, Uppsala, Sweden
P112
Inhibition of Taq Polymerase and
MMLV Reverse Transcriptase performance in presence of
polyphenolic compounds: (+)-Catechine & Epigallocatechin
Gallate (EGCG)
TICHOPAD Ales1, POLSTER Jürgen2
& PFAFFL Michael W.1 (pfaffl@wzw.tum.de)
1Institute of Physiology, 2Institute
of Biological Chemistry, TUM, 85354 Freising-Weihenstephan,
Germany,
P113
Fast identification of probes
and primers for intron spanning qPCR with the ProbeFinder
web tool.
TOLSTRUP N, CAO J, NIELSEN JB
AND RAMSING NB. E-mail: (Tolstrup@Exiqon.com)
Exiqon A/S, Bygstubben 9, DK-2950,
Denmark
P114
Four-color multiplex PCR assay
for the simultaneous detection of four allelic variants
in a closed tube using a single thermal cycler program
Ugozzoli Luis A., David Chinn,
and Keith Hamby. Bio-Rad Laboratories, Inc., 2000 A. Nobel
Drive, Hercules, CA, 94547.
P115
PCR bias in multiplex real-time
quantitative PCR
Bernhard Zimmermann, Wolfgang
Holzgreve, Sinuhe Hahn (tvk2000@hotmail.com)
Laboratory for Prenatal Medicine,
University Women’s Hospital, Basel, Switzerland.
P134
Haplotype Analysis using a Novel
Real-Time Amplification Strategy on the MJ Research Opticon
Continuous Fluorescence Detection System.
Chas Andre, Ph.D.1, Fan
Chen, Ph.D.1, Vicki Pandey1, Rich Kurtz, Ph.D. 2, and David Batey,
Ph.D. 2
chasa@bioworks.com
1 MJ Bioworks, South San Francisco, CA;
2 MJ Research, South San Francisco, CA
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Poster Session:
siRNA
P116
Investigation of the adrenomedullin
mechanism through small interfering RNAs in human cells
ALBERTIN G, FORNERIS M, CARRARO
G, NUSSDORFER GG (giovanna.albertin@unipd.it)
Department of Human Anatomy and
Physiology, University of Padua, Italy
P117
Inhibition of gene src expression
in Xenopus laevis quantified by real-time PCR
Ferjentsik Z., Šindelka R., Jonák
J. (sindelka@img.cas.cz)
Department of Protein Biosynthesis,
Institute of Molecular Genetics, Academy of Sciences
of the Czech Republic, Prague, Czech Republic
P118
Using Real-Time RT-PCR to Measure
Gene Silencing by RNAi
Lee Honigberg1, Stefany Snyder1,
Jill Spoerke1, Kathleen Shelton2
1Celera Genomics, South San Francisco,
CA 2Applied Biosystems, Foster
City, CA
P119
Accelerating Drug Target Validation
using LNA.
SAKARI KAUPPINEN1, CARSTEN ALSBO1,
PETER STEIN NIELSEN1, ANNE NØRREMØLLE2,
THOMAS LITMAN3, LIS HASHOLT2 AND JENS ERIKSEN4
(sk@exiqon.com)
1 Department of Functional Genomics,
Exiqon, Bygstubben 9, DK-2950 Vedbaek, Denmark
2 Institute of Medical Biochemistry
and Genetics, Section of Neurogenetics, University of
Copenhagen, Blegdamsvej 3, DK-2200
Copenhagen N, Denmark
3 Bioinformatics Centre, University
of Copenhagen, Universitetsparken 15, DK-2100
Copenhagen Ø, Denmark
4 Laboratory of Oncology 54O5,
Herlev University Hospital, Herlev Ringvej 75, DK-2730
Herlev,
Denmark
P120
Validation of siRNA knockdowns
by real-time quantitative PCR
TUZMEN S1, AZORSA D1, WEAVER D1,
CAPLEN N2, KALLIONIEMI O1, MOUSSES S1
(stuzmen@tgen.org)
1 Translational Genomics Research
Institute, Gaithersburg, MD, U.S.A.
2 Gene Silencing Section, Office
of Science and Technology Partnerships, Office of the
Director, Center for Cancer Research, National Cancer Institute,
NIH, Bethesda, MD, U.S.A.
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Poster Session:
High Throughput & Array Technology &
Cluster Analysis
P121
cDNA Microarray validation of
small changes in gene expression by qPCR
COVARRUBIAS M-Y1, KHAN R1, 2 and
SCHWABER J1 (yolanda.covarrubias@jefferson.edu)
1 Department of Pathology, Anatomy
and Cell Biology and Daniel Baugh Institute for Functional
Genomics/Computational Biology, Thomas Jefferson University
, Philadelphia, PA 19107 USA
2 Delaware Biotechnology
Institute and Electrical and Computer Engineering, University
of Delaware, Newark, DE 19711 USA
P122
The Applied Biosystems 7900HT
Micro Fluidic Card System with Assays-on-Demand™ Gene
Expression products.
John P. Bodeau, Sangita Parikh,
Chris Grimley, Ian Harding, Adrian Fawcett, Kathy Lazaruk,
Kathleen Shelton, Matthew Chan, Mark Wechser
Applied Biosystems, Lincoln Centre
Drive, Foster City, California, USA, 94404
Joel R. Dufresne, Louis C. Haddad,
Theresa Gerten
3M Bioanalytical Technologies
Project, 3M Company, St. Paul, Minnesota, USA, 55144
P124
Gene expression profiling using
clustering and functional annotations
KNUUTTILA JEA1, TÖRÖNEN
P2 & CASTRÉN E1 (juha.knuuttila@helsinki.fi)
Neuroscience Center, University
of Helsinki
A. I. Virtanen Institute for Molecular
Sciences, University of Kuopio
P125
Validation of microarray data
from tumor sample biopsies by short LNA enhanced qPCR
probes
KRUHØFFER M1, MOURITZEN
P2, RAMSING NB2
mouritzen@exiqon.com
1Molecular Diagnostic Laboratory,
Aarhus University Hospital, Skejby, DK8200 Aarhus N,
Denmark
2Exiqon A/S, Bygstubben 9, DK2950
Vedbaek, Denmark
P126
The real-time PCR microchip.
Victor Joseph,1 Jie Zhou,1 Jungho
Kim,2 Ventzeslav Iordanov,3 Nicke Svanvik,4 and Mikael Kubista. 4,5
(mikael.kubista@tataa.com)
1 WaferGen, Bayside Technology Center, Fremont, CA, USA,
2 Department of Mechanical Engineering, University of Maryland,
USA,
3 Delft University of Technology, Delft, The Netherlands.
4 TATAA Biocenter, Göteborg, Sweden, and
5 Chalmers University of Technology, Göteborg, Sweden.
P127
High Throughput DNA Methylation
Analysis by Base Specific Cleavage of Single Strand Nucleic
Acid and Analysis by Mass Spectrometry (MALDI-TOF)
Niels Storm1, Mathias Ehrich 2,
Sebastian Böcker2, Christiane Honisch2 and Dirk
van den Boom2
nstorm@sequenom.de
1SEQUENOM GmbH, Mendelssohnstrasse
15D, 22761 Hamburg, Germany
2SEQUENOM Inc., John Hopkins Court,
San Diego, CA 92121, USA
P128
Gene expression in SIRS and septic
patients monitored by real-time PCR using microfluidic
cards
PAHL A1, KÜNKELE S2, MÜNSTER
T2, SCHÜTTLER J2 & K BRUNE1
pahl@pharmakologie.uni-erlangen.de
1Department of Pharmacology, University
of Erlangen, D-91054 Erlangen
2Department of Anesthesiology,
University of Erlangen, D-91054 Erlangen
P129
Novel Technology Permits Three
Linear RNA Amplification Rounds and Yields Reproducible
Microarray Data with Maintained Dynamics in Differential Expression
Levels
Peter Scheinert1, Guido Krupp1
and Ludger Klein-Hitpass2
1artus GmbH, Hamburg; 2Institut
für Zellbiologie, Universitätsklinikum Essen
P130
A Whole-Genome Linkage Disequilibrium
SNP Map and Validated Assay Resource.
Leila Smith, Charles Scafe, Yu
Wang, Marion Laig-Webster, Xiaoping Su, Ryan Koehler,
Hadar Avi-Itzhak, Janet Ziegle, Lewis Wogan, Eugene Spier,
Dennis A. Gilbert, and Francisco M. De La Vega
Applied Biosystems, 850 Lincoln
Centre Dr, Foster City, CA 94404, USA
P131
Cell based assays in 384 and 1536
well formats using MosQuito and the Acumen Explorer™
WYLIE P, LEWIS R & I WHITEHALL
(ian.whitehall@ttplabtech.com)
TTP LabTech Ltd, Melbourn Science
Park, Melbourn, Hertfordshire, SG8 6EE, UK.
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