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Friday
5 March 2004
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12:00 – 14:00
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Registration
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14:00
– 14:30
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Welcome
& Opening of the Application Workshop
by Prof. M. Kubista
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14:30 – 18:00
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Lectures by the Workshop paticipating
Companies
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14:30
Oliver Geulen
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Platinum Sponsor
Roche Diagnostics,
LightCycler Devolpment Group
"The new LightCycler 2.0: An
advanced multi-channel system for rapid real-time PCR."
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14:50
Marcus Neusser
&
Luis Ugozzoli
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Gold Sponsor
R & D Bio-Rad
Laboratories
"Complete Solutions
for Real-Time PCR approaches - Bio-Rad Real-Time PCR
Systems."
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15:10
Chas Andre
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Gold Sponsor
MJ Research Inc.
"Haplotype Analysis using a Novel
Real-Time Amplification Strategy on the MJ Research Opticon Continuous
Fluorescence Detection System."
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15:30 – 16:00
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Coffee break
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16:00
Thomas Rygus
& Thomas Schild
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Silver Sponsor
Applied Biosystems (Germany)
"New tools for genetic
research: Whole genome microarrays and customized low density
solutions"
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16:20
Fabrice
Magnino
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Silver Sponsor
Stratagene Europe
"Multiplexing your assay, from
simplex to fourplex."
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16:40
Thomas Kaiser
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Corbett Research R&D, Australia
sponsered by Biotage (former Pyrosequencing)
"Optimizing Assays in real time
amplification."
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17:00
Mary Katherine
Johansson
Brian E. Caplin
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Biosearch Technologies
"Normalization using the F3 Channel
of the Lightcycler- a New Reporter Enables Multiplexing with 5’Nuclease
Probes."
"A Two-Color TaqMan Assay on the
LightCycler 1.2."
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17:20
Sarah Freshwater
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ABgene
"The effect of consumable
type on the sensitivity and reproducibility of QPCR."
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17:40
William
A. McMillan
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Silver Sponsor
Cepheid (Germany)
“Test Systems for Fast and Automated Molecular Diagnostics.”
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18:00
Bob Larsen
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BD BioSciences
"BD QZYME Assay for real-time
quantitative PCR"
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after 18:20
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Open evening
& Visit the Nightlife of Freising
(www.freising.de)
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Saturday 6th March
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08.30
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Start of the
workshop
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Workshop
agenda and organization
The 80 participants will be divided into two groups of 40 persons in
each.
One group will have seminars covering practical aspects of qPCR and
upstream processes, before lunch and hands-on experiments and data-analyses
after lunch.
The other group will have hands-on before lunch and seminars after.
Seminars:
Part 1: Introduction
Part 2: Quantification Strategies
Covering Absolute, Relative and Comparative Quantification.
How standard curves are established and the effects of efficiency estimations
for quantification results.
Several examples are presented that demonstrate the effects of erroneous
efficiency estimations.
Part 3: Nucleic acids extraction
The most common extraction methods of nucleic acids and their pros and
cons.
Quality control of nucleic acids for qPCR.
Part 4: Reverse Transcription
The available methods and strategies for reverse transcription
as well as practical considerations when performing RT.
Hands-on:
Each group of 40 persons is divided so that a small group of approximately
4 persons is stationed at each instrument.
This group sets up, programs and starts an experiment on that machine.
The groups will the rotate and get to see experimental data on the next
station/instrument and see how software, setup and analysis works on that
instrument. Also another type of experiment is performed on each instrument.
The groups rotate until they have seen all experiment/instrument types.
One instructor will be available for each station as well as company
representatives to help assist the programming of the software and answer
any questions.
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The instruments
and experiments:
LightCycler - SNP detection using FRET probes
iCycler - Duplex reaction using Molecular Beacons
myCycler - SYBR Supermix- insitu calibration
Stratagene - BEBO and BOXTO, new dyes for qPCR
Opticon 2 - SYBR Green siRNA knockdown
SmartCycler - SYBR Green
ABI Prism 7900 - TaqMan Assay
Rotorgene 3000
- QZyme (BD Biosciences)
For setup of the experiments the participants will have
approximately 1 hour and then approximately ½ hour on each platform.
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18.00
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Closing of
the Application Workshop
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The workshop is held by:
Mikael Kubista, Neven Zoric, Tzachi Bar, Cristina
Lind, Martin Bengtsson, Stephen Bustin, Michael W.
Pfaffl, Julia Herzig, Susanne Ulbrich, Christian Prgomet,
Harald Welter, and others
One instructor
will be available for each station as well as one company representatives
to help assist the programming of the software and answer any questions.
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