qPCR Application Workshop
5th & 6th March, 2004
in  Freising-Weihenstephan, Germany

For further questions about Application Workshop organisation please contact  neven.zoric@tataa.com

Workshop Oral presentations can be downloaded !

Friday 5 March 2004

12:00 – 14:00

14:00 – 14:30

Welcome & Opening of the Application Workshop by Prof. M. Kubista

14:30 – 18:00
Lectures by the Workshop paticipating Companies

Oliver Geulen
Platinum Sponsor
Roche Diagnostics, LightCycler Devolpment Group
"The new LightCycler 2.0:  An advanced multi-channel system for rapid real-time PCR."

Marcus Neusser &
Luis Ugozzoli

Gold Sponsor
R & D Bio-Rad Laboratories
"Complete Solutions for Real-Time PCR approaches - Bio-Rad Real-Time PCR

Chas Andre
Gold Sponsor
MJ Research Inc.
"Haplotype Analysis using a Novel Real-Time Amplification Strategy on the MJ Research Opticon Continuous Fluorescence Detection System."

15:30 – 16:00
Coffee break

Thomas Rygus
&  Thomas Schild

Silver Sponsor
Applied Biosystems (Germany) 
"New tools for genetic research: Whole genome microarrays and customized low density solutions"

Fabrice Magnino
Silver Sponsor
Stratagene Europe
"Multiplexing your assay, from simplex to fourplex."

Thomas Kaiser

Corbett Research R&D
, Australia

sponsered by
Biotage (former Pyrosequencing)
"Optimizing Assays in real time amplification."

Mary Katherine Johansson

Brian E. Caplin

Biosearch Technologies

"Normalization using the F3 Channel of the Lightcycler- a New Reporter Enables Multiplexing with 5’Nuclease Probes."

"A Two-Color TaqMan Assay on the LightCycler 1.2."

Sarah Freshwater


"The effect of consumable type on the sensitivity and reproducibility of QPCR."

William A. McMillan
Silver Sponsor
Cepheid (Germany)
“Test Systems for Fast and Automated Molecular Diagnostics.”

Bob Larsen

BD BioSciences
"BD QZYME  Assay for real-time quantitative PCR"

after 18:20
Open evening  &  Visit the Nightlife of Freising

Saturday 6th March

Start of the workshop

Workshop agenda and organization

The 80 participants will be divided into two groups of 40 persons in each.
One group will have seminars covering practical aspects of qPCR and upstream processes, before lunch and hands-on experiments and data-analyses after lunch.
The other group will have hands-on before lunch and seminars after.


Part 1:  Introduction

Part 2:  Quantification Strategies
Covering Absolute, Relative and Comparative Quantification.
How standard curves are established and the effects of efficiency estimations for quantification results.
Several examples are presented that demonstrate the effects of erroneous efficiency estimations.

Part 3:  Nucleic acids extraction
The most common extraction methods of nucleic acids and their pros and cons.
Quality control of nucleic acids for qPCR.

Part 4:  Reverse Transcription

The available methods and strategies for reverse transcription
as well as practical considerations when performing RT.

Each group of 40 persons is divided so that a small group of approximately 4 persons is stationed at each instrument.
This group sets up, programs and starts an experiment on that machine.
The groups will the rotate and get to see experimental data on the next station/instrument and see how software, setup and analysis works on that instrument. Also another type of experiment is performed on each instrument.
The groups rotate until they have seen all experiment/instrument types.

One instructor will be available for each station as well as company representatives to help assist the programming of the software and answer any questions.

The instruments and experiments:

LightCycler - SNP detection using FRET probes

iCycler - Duplex reaction using Molecular Beacons

myCycler - SYBR Supermix- insitu calibration

Stratagene - BEBO and BOXTO, new dyes for qPCR

Opticon 2 - SYBR Green siRNA knockdown

SmartCycler - SYBR Green

ABI Prism 7900 - TaqMan Assay

Rotorgene 3000 - QZyme (BD Biosciences)

For setup of the experiments the participants will have approximately 1 hour and then approximately ½ hour on each platform.

Closing of the Application Workshop

The workshop is held by:

Mikael Kubista,   Neven Zoric,   Tzachi Bar,  Cristina Lind,  Martin Bengtsson,  Stephen Bustin,  Michael W. Pfaffl,  Julia Herzig,  Susanne Ulbrich,  Christian Prgomet,  Harald Welter,  and others

One instructor will be available for each station as well as one company representatives to help assist the programming of the software and answer any questions.

Workshop participating companies :



ABI  Instruments



Bio-Rad  Instruments & Reagents



Biosearch-Technologies  Probes





Eppendorf   qPCR Reagents
&  Instruments ( centrifuges  & pipetting sets )


Corbett Research Instruments

Rotor-Gene   and    CAS-1200 Robot



Roche  Instruments & Reagents

Stratagene  Instruments

MJ Research Instruments



1st International qPCR Symposium  &  Application Workshop © 2003 by Michael W. Pfaffl 

For further questions about Application Workshop organisation please contact  

For Workshop registration contact   susanna.wirth@tumtech.de
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