qPCR  2005
2nd International qPCR Symposium
& Industrial Exhibition
TATAA Application Workshop  &  qPCR Matrix Workshop

The whole story of quantitative PCR  –  from Tissue Preparation to BioInformatics

5 - 9th September  2005  in  Freising-Weihenstephan,                
Technical University of Munich, Physiology Weihenstephan, Germany

Freising,  2005
Dear colleagues,
dear researchers,

dear company representatives,

on behalf of the Organisation Committee and the Scientific Board it is great pleasure to invite you to the 2nd International qPCR Symposium & Application Workshop to be held September 5-9, 2005, at the Center of Life Science in Freising Weihenstephan, Technische Universität München (Germany). The great international interest in the qPCR 2004 - 1st International qPCR Symposium & Application Workshop by 465 participants from 44 countries and 32 international companies in the qPCR Industrial Exhibition led us to the decision to repeat it in 2005. The event will focus on all aspects of qPCR technology and its applications in research and diagnostics. Leading academic researchers and industrial contributors in the field will be participate in the symposium, which will be an arena for fruitful discussions between researchers of different backgrounds. Title of the Symposium talks. The Symposium, the Industrial Exhibition and two types of associated Workshops offer an overview of the present knowledge and future developments in qPCR technology and its wide applications. The subtitle of the qPCR 2005 events is:

The whole story of quantitative PCR – from Tissue Preparation to BioInformatics

The event is divided in
1.    qPCR Symposium from  5 – 7th September,
2.    qPCR Industrial Exhibition from  5 – 7th September
3.    qPCR Workshop from  7 – 9th September  ( TATAA application workshop and the qPCR Matrix Workshop )  
The location is the central lecture hall and the foyer at TUM (Technical University Munich) in Freising Weihenstephan. We are part of the biggest Biotech Cluster in Germany and Europe, located close to the Munich airport, and in the heart of Bavaria.

The scientific organization will be managed by a similar team like last year:

Scientific board:

Stephen Bustin
Prof. of Molecular Science QM, School of Medicine, London, UK
Mikael Kubista
Prof. of Biotechnology, MultiD Analyses AB, Gothenburg, Sweden
Vladimir Benes
EMBL, Genomics Core Facility, Heidelberg, Germany
Neven Zoric
Coordinator of TATAA Biocenter, Sweden     (coordinator TATAA application workshop)
Heinrich H.D. Meyer
Prof. of Physiology, Weihenstephan, Germany
Michael W. Pfaffl
Reader in Physiology, Weihenstephan, Germany
scientific coordinator of the Symposium and the Matrix Workshop  qPCR2005@wzw.tum.de

Special guest:

Russell G. Higuchi
Pioneer in real-time PCR, Associate Director of the Human Genetics Department,
Roche Molecular Systems, Alemada, CA, USA

Event organization:
Ulrich Wild, TUM-Tech GmbH Munich     ulrich.wild@tumtech.de

I am looking forward meeting you in September at the Symposium in Freising-Weihenstephan.
Michael Pfaffl
Sympoium Chair

NEWS   -   qPCR 2005   -   NEWS   -   qPCR 2005   -   NEWS   -   qPCR 2005   -   NEWS

Talk Photo Gallery

Workshop Photo Gallery

Exhibition Photo Gallery

Welcome Photo Gallery

Dinner Photo Gallery

Dancing Photo Gallery

    • => the TATAA qPCR application workshop is fully booked by 20 people !   ( 22nd July 2005 )
    • => please register and participate in the qPCR Matrix Workshop   is fully booked by 60 people !   ( 31th August 2005 )
    • => organisation of the qPCR Matrix Workshop on Monday late afternoon ( 17:00 - 18:30 ) =>  overview
      • 11 interesting sessions will be presented in the qPCR Matrix Workshopov from Wednesday till Friday,
      • at least 8 different real-time cyclers ( new Roche LightCycler 480, new Eppendorf RealPlex, new Bio-Rad iQ5 Cycler, Rotor-Gene 3000 by Corbett Research, Applied Biosystems 7900, Stratagene MX cycler(s), SmartCycler, etc. )
      • presentation of high-throughput technologies using pipetting- and nucleic acid extraction robots (by Eppendorf, Applied Biosystems, and Corbett Research),
      • lots of new real-time applications ( SYBR Green, fast-PCR, multiplex-qPCR, one-step and two-step qRT-PCR systems, low density arrays, universal probe library, genotyping, etc. ) will be presented by Applied Biosystems, Qiagen, Roche, Invitrogen, Promega, etc.
      • presentation of new BioInformatics tools for relative quantification and statistical analysis of real-time data, by Jo Vandesomplete, Jan Hellemans, Mikael Kubista, Michael W. Pfaffl and co-workers, 
We welcome our international speakers and symposium participants at the qPCR 2005 Event !
Europe USA Sweden France Slovenia Israel Portugal
Austria Canada Denmark Germany Finland UK
Czech Republic Australia Belium Italy Saudi Arabia Switzerland Ghana
Norway Spain Thailand Russia Albania India Brazil Taiwan
Turkey Nigeria   Nepal New Zealand  Egypt Sierra Leone Poland Netherlands
United Arab Emirates Peru China Greece Yugoslavia Estonia Senegal Tunesia

Important dates:
The official conference and workshop language is ENGLISH. No simultaneous translation will be provided.
All oral presentation, poster and abstarcts must be presented in English.
All scientific contributions will be published in the qPCR 2005 Symposium Proceedings     ISBN 3000166874

Abstract submission deadline:
new deadline   =>  31st  July  2005    (submission now closed)

Abstract submission:
internet based abstract submission platform =>   https://www.wzw.tum.de/conftool/

Please indicate if you want to present either a Poster or give an oral Presentation via MS PowerPoint.

  • Until Spring 2006 the PDF download of the contributions is password protected and only accessible for Symposium and Workshop participants.
  • The abstract should be a one-page document based on unpublished material and written in good standard English. The text should clearly state objectives, methodology, results, and conclusion. Simple tables and graphs are allowed.
  • Poster will be DIN A0 (portrait format :120 cm/48 inch high x 90 cm/35 inch wide)
  • Presentation of every communication at the Conference is subjected to formal registration of at least one co-author.

internet based registration platform =>   https://www.wzw.tum.de/conftool/

The registration fee includes:

registration fees

  • Proceedings:   printed proceedings ISBN 3000166874 containing all the poster and talk abstracts.
  • Online access:  all presentation slides and posters will be shown online on the symposium homepage, in a password protected area.
  • Full catering service at the Symposium:  includes all kind of drinks (coffee, tea, alcoholic, and non-alcoholic), snacks, three lunch meals (Mo. - Wed.) and two events: a Poster-Party on Monday evening with live music (sponsored by and Roche Aplied ScienceEppendorf) and a downtown Evening-Event at Tuesday in Freising with a variety of delicious buffets. A detailed qPCR 2005 welfare guide will be published soon !
  • Full catering service at the Workshops:  includes all kind of drinks (coffee, tea, and non-alcoholic), snacks, and three lunch meals (Wed. - Fri.).

Symposium Proceedings:

The symposium proceedings ISBN 3000166874 including all Abstract, Posters and Oral Presentations. Proceedings will be published as FULL PDF PRESENTATIONS on the qPCR2005.Gene-Quantification.info web page. As wanted by the authors the PDF download of the contributions is password protected and only accessible for Symposium or Application Workshop participants, until spring 2006.

qPCR Symposium

The symposium will focus on approximately 35 lectures presented by internationally recognised experts in their field. The emphasis will be on unbiased, didactic information exchange.  Internationally renown speakers will be participating in a lively and exciting programme enabling the valuable exchange of information in the qPCR field. One third of the talks will be presented by selected invited speakers, one third will be selected from the submitted abstracts and one third will be presented by qPCR company R&D representatives. All scientific contributions will be published in the qPCR 2005 Symposium Proceedings.

The sessions will be grouped like the work flow in a scientific lab - from RNA/DNA tissue preparation to qPCR BioInformatics applications. Starting with sampling, NA isolation, RT, qPCR, CP determination and how to deal with the generated results. New methods of quantification strategies, normalization procedures, new calculation algorithms and exact nucleic acid quantification in combination with array technology will be presented. Depending on the submitted abstract we want to have a GMO and Food analysis session.

Title of the Symposium talks

Symposium and  Poster sessions:

Pre-analytical Steps
sampling technologies, DNA / RNA purification, DNA / RNA quality control, Reverse Transcription, efficiency of DNA / RNA extraction, influence on real-time PCR, etc.......
optimisation of the real-time reaction, inhibition of negative effects, new dyes, increase real-time PCR efficiency, optimisation of multiplexing, etc........
establishment of DNA / RNA standards, comparability between runs, tests, labs and countries, national and international studies on qPCR standardisation, etc.......
new types of normalisation, one housekeeping gene  vs. multiple housekeeping genes, new relative quantification software applications, etc........
Array Verification
expression profiling, comparability of cDNA array results with real-time RT-PCR, comparability of real-time RT-PCR with other methods,   etc......
New Applications
new quantification methods, high throughput applications, CHIP technology, multiplexing, new dyes or probe technologies, new quantification strategies, etc.......
GMO Analytics & Food Hygiene
GMO problematic, food testing, microbiology, validation of methods, special plant and food extraction methods, etc.....
new software applications, calculation of relative expression, exact determine of real-time PCR efficiency, optimal CP determination, mathematical algorithms, data management, statistics in real-time PCR, 3D data visualisation,  etc......

qPCR Workshops

We plan to perform two types of workshop. One will be like a classical qPCR Application Workshop (type 1) organized by the TATAA Biocenter in Sweden (www.tataa.com), and the other is a new type of Matrix Workshop (type 2) where people rotate and can visit 6 different workshop sessions. Both qPCR Workshop typess will be held from afternoon 7th till late afternoon 9th September 2005 and will be divided in two different workshop types. The students can visit either the type 1 or the type 2.

Researchers and qPCR companies will be responsible for their own equipment: all hardware, software, kits and disposables. The organizers at the TUM-Weihenstephan can provide four different rooms (P1, P2, P3 and P4) with different number of benches (from 7 to 17 benches, each fitting 4-6 students 1-2 and workshop session instructors), chairs, electricity, W-LAN, fridges, freezers, crushed ice, water, and…...

Each session will be held on two lab-benches, one for sample preparation and one for the qPCR applications.

classical qPCR workshop, presented by TATAA Biocenter

Practical room number 2 - Plan of room P2

The qPCR workshop offers hands-on training by leading experts in qPCR from TATAA Biocenter. Major probe technologies will be demonstrated, leading and emerging instrument platforms will be presented, and advanced primer and probe design programs will be available. Hands-on training will be provided on quantitative gene expression, including design and optimization of both RT and qPCR protocols. Participating companies are asked to support the workshop with cyclers and kits. To support of the with PCR cyclers, qPCR kits, consumables, centrifuges or pipettes please contact Neven Zoric at the TATAA Biocenter   Neven.Zoric@tataa.com

NEW type of qPCR Matrix Workshop, presented by the qPCR companies itself

Practical room number 1, 3 and 4   =>  Plans of  P1  and  P3  and  P4

New type of interactive workshop.
Researchers and qPCR companies are addressed to present their technologies (cycler, chemistry, kits or software applications) by their own product specialists.
Each student can visit 6 different session types at the 6 time frames during the qPCR workshop. After finishing a session the students move to the next bench and session type.
In each time frame 4-6 students can be educated by one or two instructors in a defined subject.
Each session can use two benches, one for sample preparation and one for the qPCR applications.
Each workshop session will last for 3 hours.
Three sessions will be presented at one day.
The students can choose and book the sessions on Monday afternoon (17:00 - 18:30) registartion desk.

The session instructors from the companies are asked to generate interesting sessions in which the student can learn about one distinct application or can solve one special problem. For session ideas arrangements please contact M. W. Pfaffl   qPCR2005@wzw.tum.de

qPCR Industrial Exhibition

An industrial exhibition will be held during the qPCR Symposium from 5 – 7th September in the foyer of the central lecture hall complex (green frame) and in two side rooms S1 and S2 (blue frame).  According to the booth location we have 26 booth in different prize categories. They are all grouped around the central lecture halls H14 and H15 and fits up to 650 people.

Parallel to the Symposium an industrial Exhibition will take place along with the congress, where an intensive conversation with numerous companies is possible (real-time PCR Cyclers,  Kit Producers,  Nucleic Acid Purification Systems, Enzyme Producers, Plasticware Suppliers, etc.)
=>  Link to the Industrial Exhibition

To book a booth and for further details about the industrial exhibition, e.g. exhibition contract, technical management, stand allocation, electronic installations, internet connection (W-LAN), etc. we are pleased to answer your questions.
Please contact Dr. Ulrich Wild from TUM-Tech GmbH Munich ulrich.wild@tumtech.de

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for further questions about the scientific meeting contact  qPCR2005@wzw.tum.de

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