Symposium Posters

Time:  5th Sep 2005, 18:30  -  5th Sep 2005,   20:00
Session Chair:   Prof. Dr. Heinrich H.D. Meyer
Location:   Student Cafeteria
Poster Sessions:


Poster Session:   Pre-Analytical Steps

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P01

A New Mini-prep and Rapid DNA Extraction Method.

Asadollah Ahmadikhah (ahmadikhah@narod.ru)
Timiriazev Agricultural University, Russian Federation.

P02

The complex study of storage and handling of human placenta tissue samples.

Martina Pejznochova, Marketa Tesarova, Tomas Honzik, Hana Hansikova, Jiri Zeman (martina.pejznochova@centrum.cz)
Charles University, Czech Republic.


P03

RNeasy FFPE Kit - RNA Isolation from Formalin-Fixed Paraffin-Embedded Tissue.

Martin Schlumpberger, Silke v. Ahlfen
(martin.schlumpberger@qiagen.com)
QIAGEN GmbH, D-40724 Hilden, Germany.


P04

Bone marrow samples treated for cytogenetic analysis are not suitable to PCR amplification.

Barbara Rocca, Silvia Calatroni, Elisabetta Lugano, Ilaria Giardini, Jessica Quarna, Marina Boni, Paola Maria Cavigliano, Rita Zappatore, Marilena Caresana, Paolo Bernasconi (s.calatroni@smatteo.pv.it)
IRCCS Policlinico S.Matteo - Division of Hematology, Italy.



Poster Session:   New Application

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P05

Expression profiling of early development stages of Xenopus laevis.

Radek Sindelka, Zoltan Ferjentsik, Jiri Jonak (sindelka@img.cas.cz)
IMG AS CR, Czech Republic.


P06

Two-color multiplex assay for the identification of Orthopoxes viruses wih Real-time LUX PCR.

Mohamed Aitichou 1, Sandrine Javorschi-Miller 2, Sofi Ibrahim 1, Mark Andersen 2 (sandrine.javorschi@invitrogen.com)
1: Virology Division, United States Army Medical Research Institute of Infectious Diseases, United States.
2: Invitrogen, United States.



P07

Global RNAi phenotype analysis for cancer drug target identification and validation by qRT-PCR.

TUZMEN S, AZORSA D, EVANS D, KIEFER J, QUE Q, BITTNER M, KALLIONIEMI O, TRENT JM, VON HOFF D, MOUSSES S (stuzmen@tgen.org)
Translational Genomics Research Institute (TGen), U.S.A.


P08

Universal ProbeLibrary: A new concept for real-time qPCR assays.

Ralf P. Mauritz1, Peter Mouritzen2, Henrik M. Pfundheller2, Niels Tolstrup2 (ralf.mauritz@roche.com)
1: Roche Diagnostics GmbH, Roche Applied Science, Nonnenwald 2, D-82377 Penzberg, Germany.
2: Exiqon A/S, Bygstubben 9, DK-2950 Vedbaek; Denmark.


P09

Mapping of homozygous deletions in chromosome 3p regions affected in major epithelial tumors using real-time PCR.

V.Senchenko(1, 2), E.Braga3, J.Liu2, V.Loginov3, V.Kashuba2, R.Garkavtseva4, N. Mazurenko4, F.Kisselev4, L.Kisselev1, E.Zabarovsky2 (versen@eimb.ru)
1: Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia.
2: MTC, Karolinska Institute, Stockholm, Sweden.
3: Russian State Genetics Center, Moscow, Russia.
4: Blokhin Cancer Research Center, RAMS, Moscow, Russia.


P10

DNA isolation from FTA Mini Cards as a high-throughput method for genotyping of Glutathione S- transferase P1 polymorphisms in workers with asbestosis.

Franko A 1, Toplak N 2, Dodic-Fikfak M 1, Dolzan V 3
(omega@omega.si)
1: Clinical Institute of Occupational Medicine, University Medical Centre, Ljubljana, Slovenia.
2: Omega d.o.o., Dolinškova 8, Ljubljana, Slovenia.
3: Institute of Biochemistry, Faculty of Medicine, Ljubljana, Slovenia.

 
P11

qPCR in the elucidation of an insect-endosymbiont relationship.

Caroline Matthew, Simon Young, Alistair Darby, Sue Welburn (c.z.matthew@sms.ed.ac.uk)
Centre for Tropical Veterinary Medicine, University of Edinburgh, United Kingdom.


P12

LightCycler® 480 System: a novel real-time PCR platform for high-throughput qPCR and genotyping.

A. Degen, M. Hoffmann, G. Tellmann and R. Seibl (gudrun.tellmann@roche.com)
Roche Diagnostics GmbH, Roche Applied Science, Nonnenwald 2, D-82377 Penzberg, Germany.
(presentation of LightCycler® 480 System continued on P13  and P14)

 
P13

LightCycler® 480 System: a novel real-time PCR platform for high-throughput qPCR and genotyping.

A. Degen, M. Hoffmann, G. Tellmann and R. Seibl (michael.hoffmann@roche.com)
Roche Diagnostics GmbH, Roche Applied Science, Nonnenwald 2, D-82377 Penzberg, Germany.
(presentation of LightCycler® 480 System continued on P14)
 

P14

LightCycler® 480 System: a novel real-time PCR platform for high-throughput qPCR and genotyping.

A. Degen, M. Hoffmann, G. Tellmann and R. Seibl (anja.degen@roche.com)
Roche Diagnostics GmbH, Roche Applied Science, Nonnenwald 2, D-82377 Penzberg, Germany.


P15

REAL-TIME PCR GENE EXPRESSION ANALYSIS TO EVALUATE ADDITIONAL MOLECULAR MARKERS IN ACUTE PROMYELOCYTIC LEUKEMIA (APL) WITH MULTIPLE RELAPSES.

Silvia Calatroni, Paolo Bernasconi, Barbara Rocca, Elisabetta Lugano, Marina Boni, Paola Maria Cavigliano, Ilaria Giardini, Rita Zappatore, Marilena Caresana, Jessica Quarna, Mario Lazzarino. (s.calatroni@smatteo.pv.it)
IRCCS Policlinico S.Matteo - Division of Hematology, Italy.


P16

A potential role for hydrocortisone in the positive regulation of IL-15-activated NK cell proliferation and survival.

Sonia A. Perez, Louisa G. Mahaira, Fillio J. Demirtzoglou, Panagiota A. Sotiropoulou, (lmahaira@yahoo.gr)
Cancer Immunology and Immunotherapy Center, Saint Savas Cancer Hospital, Greece.


P17

Advanced Real-Time PCR Assays on the LightCycler480 instrument.

Jasmin Dehnhardt, Sascha Gille, Cornelia Götz, Olfert Landt, Uli Lass, Andrea Leider, Dario Papi, Roger Petersen, Christine Woitkowiak (olandt@tib-molbiol.de)
TIB Molbiol Syntheselabor GmbH, Deutschland.


P18

MicroRNA expression signature in human glioblastoma multiforme brain tumor.

Astrid Potratz, Dana Ridzon, Ruoying Tan, Julie Nguyen, Adam Broomer, and Caifu Chen (Astrid.Potratz@eur.appliedbiosystems.com)
R&D, Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA 94404, USA.


P19

Expression and localisation of fibroblast growth factor (FGF) family members in porcine antral follicles.

Steinberg V, Berisha B, Pfaffl MW, Schams D (steinber@wzw.tum.de)
Physiology Weihenstephan, Technical University Munich, Weihenstephaner Berg 3, D-85354 Freising-Weihenstephan, Germany.

 
P20

Expression of mRNA for Apoptotic and Anti-apoptotic Factors during Oestrous Cycle and Pregnancy in Bovine Corpus Luteum.

Berisha B, Fürst R, Kliem H, Meyer H.H.D, Schams D (berisha@wzw.tum.de)
Physiology Weihenstephan, Technical University Munich, Weihenstephaner Berg 3, D-85354 Freising-Weihenstephan, Germany.


P21

Quantitative expression of bone morphogenetic protein and bone matrix protein in human carotid plaque.

Corey Goldman, Sonia H. Montenegro, Christopher White, Richard N. Re (smontenegro@ochsner.org)
Ochsner Clinic Foundation, New Orleans, LA United States. Department of Cardiology, Molecular Immunogenetics Laboratory.


P22

Immunerelevant gene expression of primary bovine epithelial cells in response to different mastitis pathogens.

Peter Reith, Olga Wellnitz (preith@wzw.tum.de)
Technische Universität München, Institut für Physiologie, Weihenstephan, Deutschland.

 
P23

Characterization of a novel suspension cell culture system for bovine oviduct epithelial cells.

Regine Rottmayer 1, Susanne E. Ulbrich 2, S. Koelle 3, F. Sinowatz 3, R. Einspanier 4, H.H.D. Meyer 2, E. Wolf 1, S. Hiendleder 1 (ulbrich@wzw.tum.de)
1: Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilian-University Munich, Munich, Germany.
2: Physiology-Weihenstephan, Deutschland,
3: Institute of Veterinary Anatomy II, Ludwig-Maximilian University Munich, Germany,
4: Institute of Veterinary Biochemistry, Free University of Berlin, Berlin, Germany.


P24

Short term effects on pro-inflammatory cytokine, lactoferrin, and CD14 mRNA expression levels in bovine immuno-separated milk and blood cells treated by LPS.

Christian Prgomet, Hande Sarikaya, Rupert M. Bruckmaier, Michael W. Pfaffl (Prgomet@web.de)
Lehrstuhl für Physiologie TU München, Deutschland.


P25

Expression of bovine ABC transporters - potential role of ABCA1, ABCG5 and ABCG8 in cholesterol transport in the mammary gland.

Carolin Farke, Enrique Viturro, Christiane Albrecht (cfarke@wzw.tum.de)
Technical University Munich, Physiology Weihenstephan, Germany.


P26

Effects of Anabolic Sex Hormones on Gene Expression in Bovine Liver – Differential Gene Expression Profiling via a candidate gene approach qRT-PCR.

Vanessa M. Walf, Martina Reiter, Michael W. Pfaffl, Heinrich H.H.D. Meyer (martina.reiter@wzw.tum.de)
Physiology, Technical University Munich, Deutschland.


P27

IGF-1 IN BOVINE SERUM AND LEUKOCYTES.

Avo Karus, Zinaida Saprokina (akarus@eau.ee)
Estonian Agricultural University, Estonia.


Poster Session:   Normalization

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P28

Novel reference genes for normalization of real-time PCR data in normal human tissues and an application to gene expression profiling.

Viktor Lakics, Alessandro Lanza, Eric Karran, Frank Boess (vlakics@lilly.com)
Eli Lilly and Company Ltd, Erl Wood Manor, Sunninghill Road, Windlesham, Surrey, GU20 6PH, UK.


P29

mRNA Expression of Apoptotic and Anti-apoptotic Factors during PGF2a Induced Luteolysis in Bovine Corpus Luteum.

Kliem H, Kraetzl D.W, Schams D, Berisha B (kliem@wzw.tum.de)
Physiology Weihenstephan, Technical University Munich, Weihenstephaner Berg 3, D-85354 Freising-Weihenstephan, Germany.


P30

Mutations Induced at the Promotor Reguion of the myc gene due to Dual exposure to ionizing radiation and N-nitroso-urea.

Soheir Saad Korraa (soheirskorraa@yahoo.com)
National Centre for Radiation Research and Technology- Atomic Energy Authority of Egypt, Egypt.


P31

Locked Nucleic Acid (LNA) Single Nucleotide Polymorphism (SNP) Genotype Analysis and Validation Using Real-Time PCR.

Matthew P. Johnson, Laria M. Haupt, Thomas Kaiser and Lyn R. Griffiths (thomas.kaiser@corbettresearch.com)
Genomics Research Centre, School of Health Science, Griffith University Gold Coast, PMB 50, Gold Coast Mail Centre, QLD 9726, Australia.



Poster Session:   Optimization

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P32

New information on detection of Plum pox potyvirus using real time PCR.

Irena Mavric 1, Natasa Toplak 2, Mojca Virscek Marn 1 (irena.mavric@kis.si)
1: Agricultural Institute of Slovenia, Hacquetova 17, Ljubljana, Slovenia.
2: Omega d.o.o., Dolinskova 8, Ljubljana, Slovenia.


P33

Analysis of best primer and reverse transcriptase combination for the RT reaction using low amounts of bovine oocyte total RNA.

Marc Boelhauve, Fabiola Freitas Paula-Lopes, Eckhard Wolf (boelhauv@lmb.uni-muenchen.de)
Ludwig-Maximilians University Munich, Germany.


P34

Design and validation of a robust diagnostic assay (prv-1 gene) based on real-time RT-PCR.

Verena Bohle, Peter Haeusler (phausler@web.de)
private, Deutschland.


P35

Simple Qualitative and Quantitative Methods for Measuring Residual Activity of “Hot-Start” Enzyme Preparations.

Ralph Somack, Shawn Hodges, Vicki Pandey and Doug Bost (somackrn@appliedbiosystems.com)
Applied Biosystems, United States.

 
P36

Distribution of mRNA transcripts in single cells determined by quantitative RT-PCR.

Martin Bengtsson1, Anders Ståhlberg2, Patrik Rorsman(1, 3), Mikael Kubista2 (martin.bengtsson@med.lu.se)
1: Department of Experimental Medical Science, Lund University, Lund, Sweden.
2: Department of Chemistry and Biosciences - Molecular Biotechnology, Chalmers University of Technology and TATAA Biocenter, Göteborg, Sweden.
3: The Oxford Centre for Diabetes, Endocrinology and Metabolism, The Churchill Hospital, Oxford, England.


P37

A single-tube PCR with MGB Eclipse probes for detection of SHV-type extended-spectrum beta-lactamases (ESBLs).

Ekimov A.N.1, Edelstein M.V.1, Kaiser T 3, Belousov E.S.2. (thomas.kaiser@corbettresearch.com)
1: Institute of Antimicrobial Chemotherapy, Smolensk, Russia.
2: Epoch Biosciences, Bothell, WA, USA.
3: Corbett Research, Sydney, Australia.


Poster Session:   Standardization

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P38

Association of Biomolecular Resource Facilities (ABRF) Nucleic Acid Research Group Study: A Comparison of Real-Time RT-PCR Technique, Chemistries and Hardware in Laboratories Utilizing the Same Assay.

Pamela Scott Adams1, Gregory Shipley2, Brian Holloway3, Yongde Bao4, Stephen Bustin5, Deborah Grove6, Anthony Yeung7 (sadams@northnet.org)
1: Trudeau Institute, Saranac Lake, NY, USA 2:UTHSC-Houston, Houston, TX, USA 3:CDC, Atlanta, GA, USA 4:University of Virginia, Charlottesville, VA, USA 5:Queen Mary’s School, University of London, UK 6:Penn State University, University Park, PA, USA 7:Fox Chase Cancer Center, Philadelphia, PA, USA.


P39

Development of a generic inhibition control for real-time PCR assays.

LEONARDO B. PINHEIRO 12, Moreland D. Gibbs 1, Graham Vesey 2, Zena Kassir 3, Kate R. Griffiths 3, Kerry R. Emslie 3 and Peter L. Bergquist 1 (lpinheir@els.mq.edu.au)
1: Macquarie University, North Ryde, NSW, Australia.
2: BFT Pty Ltd, North Ryde, NSW, Australia.
3: National Measurement Institute, Pymble, NSW, Australia.


P40

Expression of Active BCR Related (ABR) gene in Chronic Myeloid Leukemia (CML).

Carolina Y. Namasu 1, Fernando F. Costa 1, Fernando Lopes Alberto(1, 2) (flalberto@gmail.com)
(1) Hemocenter of the Satate University of Campinas, Brazil (2) Fleury Institute, Brazil.


P41

Quantitative PCR assay set-up on the Eppendorf ep Motion 5070 using RealMasterMix Probe.

Andres Jarrin (jarrin.a@eppendorf.de)
Eppendorf AG, Deutschland.

Poster Session:   BioInformatics

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P42

Absolute Quantification in Real-time PCR by Nonlinear Regression Analysis - is the Standard Curve Obsolete?

Rasmus Goll, Trine Olsen, Guanglin Cui, Jon Florholmen (rasmus.goll@unn.no)
University of Tromso, Norway.


P43

An Advanced, Web-Based, Software Program for Real-Time PCR Sequence Design.

Ben Sowers (ben@biosearchtech.com)
Biosearch Technologies, Inc., United States.


P44

AssayBank: A simple database for tracking QPCR assay performance for small-scale labs.

Lisbeth Winer (1) and Kristin B. Andersson (1, 2)
(k.b.andersson@medisin.uio.no)
1: Institute for Experimental Medical Research, Ullevaal University Hospital, University of Oslo.
2: Center for Heart Failure Research, Faculty of Medicine, University of Oslo.


P45

REST 2005 - a new standalone software for efficiency-corrected relative quantification.

Matthew Herman1, Valin Reja1, Michael W. Pfaffl2
(michael.pfaffl@wzw.tum.de)
1: Corbett Research, Mortlake, NSW, Australia.
2: Technical University Munich, Freising Weihenstephan, Deutschland.


P46

Theoretical modeling qPCR with chemical thermodynamics.

Mei Xu (mmmxxxuuu2004@yahoo.com)
Gene21st, United States.

Poster Session:   GMO & Food Hygiene

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P47

Gene expression analysis of enterohaemorrhagic Escherichia coli virulence using qRT-PCR assay.

Maira Jessica Medellin-Peña(1, 2), Aurélien Deriencourt 2, Mansel W. Griffiths 2 (mmedelli@uoguelph.ca)
1: University of Guelph, ON. Canada.
2: Canadian Research Institute for Food Safety (CRIFS), Guelph, ON. Canada.

 
P48

Direct quantification of pathogens in food samples by filtration and qPCR without the need for enrichment or DNA purification.

Petra Wolffs, Mansel Griffiths (pwolffs@uoguelph.ca)
Canadian Research Institute for Food Safety, Canada.


P49

RAPID DETECTION OF Campylobacter jejuni BY IMMUNOMAGNETIC SEPARATION AND REAL-TIME qPCR.

Rocío Morales-Rayas, Petra F.G Wolffs, and Mansel W. Griffiths (rmorales@uoguelph.ca)
Canadian Research Institute for Food Safety. University of Guelph. 43 McGilvray St, Guelph, Canada, N1G2W1.


P50

Comparison of an IMS-real-time PCR-method with a cultural reference method for the detection of Salmonella spp. in meat.

Angelika Christiane Notzon, Johann Bauer
(Angelika.Notzon@wzw.tum.de)
Lehrstuhl für Tierhygiene, TUM, WZW, Deutschland.


P51

Quantification of GMOs in food: Assessment of measurement uncertainty (MU).

Isabel Taverniers, Marc De Loose (i.taverniers@clo.fgov.be)
Department of Plant Genetics and Breeding, DvP-CLO, Melle, Belgium.


P52

Detection and quantification of genetically modified organisms (GMOs) in food.

Isabel Taverniers, Friedle Vanhee, Marc De Loose (i.taverniers@clo.fgov.be)
Department of Plant Genetics and Breeding, DvP-CLO, Melle, Belgium.


P53

GMO detection/quantification in different food matrices: not an easy task.

Dejan Stebih, Tina Demsar, Natasa Sever, Katarina Cankar, Kristina Gruden, Jana Zel (dejan.stebih@nib.si)
National Institute of Biology, Slovenia.


P54

Feeding genetically modified Bt176-maize – no effects on selected ruminal bacteria.

Patrick Gürtler, Steffi Wiedemann, Christiane Albrecht (patrick.guertler@wzw.tum.de)
Technical University Munich, Physiology, Germany.


P55

Confirmation and Quantification of Leptospiral Burden in a Human Population using Real-Time qPCR.

Louise Sefton, Eddy Segura, Christian Ganoza, David Batey, Joseph Vinetz, and Amy Tam (louise_sefton@bio-rad.com)
Bio-Rad Laboratories, United States.

 
P56

No Impact of feeding genetically modified maize on the immune system of cows.

Steffi Wiedemann, Claudia Dummer, Bodo Lutz, Tamara Stelzl, Christiane Albrecht (wiedemann@wzw.tum.de)
Physiology Weihenstephan, TU Muenchen, Freising.


P57

In situ study on the time-dependent degradation of recombinant maize DNA in the bovine rumen.

Steffi Wiedemann, Bodo Lutz, Christiane Albrecht (wiedemann@wzw.tum.de)
Physiology Weihenstephan, TU Muenchen, Freising, Germany.

Poster Session:   Array Verification

Time: 5th Sep 2005, 18:30:00 - 5th Sep 2005, 20:00:00
Session Chair: Prof. Dr. Heinrich H.D. Meyer
Location: Student Cafeteria


P58

Validation of a bacterial 70mer oligo microarray using the Exiqon human probe library.

Mads Bennedsen1, Christel Garrigues2 and Martin B. Pedersen2 (mads.bennedsen@dk.chr-hansen.com)
Identification section (1) and department of Genomics & Strain Development(2), Chr. Hansen, Denmark.


P59

Genome Wide Expression Profiling of Paired Cancerous.

Astrid Potratz, Kelly Li, Irene Lui, and Gary P. Schroth (Astrid.Potratz@eur.appliedbiosystems.com)
Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404.


P60

Validation of a transcriptomics analysis using real-time qPCR: Deciphering embryo-maternal communication.

Susanne E. Ulbrich 1, C. Klein 2, S. Bauersachs (2, 3), R. Einspanier 4, E. Wolf (2, 3), H.H.D. Meyer 1 (ulbrich@wzw.tum.de)
(1)    Physiology-Weihenstephan, Deutschland
(2)    Institute of Molecular Animal Breeding and Biotechnology
(3)    Laboratory for Functional Genome Analysis (LAFUGA), Ludwig-Maximilian-University Munich, Munich, Germany
(4)    Institute of Veterinary Biochemistry, Free University of Berlin, Berlin, Germany.


P61

ENGL - The European Network of GMO Laboratories.

Sven Pecoraro (sven.pecoraro@lgl.bayern.de)
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, D-85764 Oberschleißheim, Germany.
Any changes ???   =>   please contact the scientific organizer Michael W. Pfaffl via  qPCR2005@wzw.tum.de