Symposium Agenda                    qPCR 2005 Symposium Homepage

Central lecture halls complex in Freising – Weihenstephan, Technical University Munich (TUM), 85354 Freising, Germany
Symposium & Workshops    agenda
Sessions & Workshops   overview

TATAA Application Workshop ( fully booked )
qPCR Matrix Workshop  ( still vacancy )


Sunday  4th September 2005

13:00 – 18:00     Built-up for Industrial Exhibition

15:00 – 18:00    Arrival & Registration

Monday  5th September 2005

Welcome  &  Opening of the Symposium
Lecture hall   HS 14

08:00 – 10:00    Built-up for Industrial Exhibition

08:00 – 10:00    Arrival & Registration

09:00 – 10:00    Welcome Coffee & Tea

10:00    Welcome & Opening of the Symposium.
Michael W. Pfaffl   &   Neven Zoric
Scientific coordination of the qPCR 2005 Symposium & TATAA Application Workshop

10:10    Welcome at the Center of Food & Life Science in Freising Weihenstephan.
Prof. Dr. Dr. h.c. mult. Wolfgang A. Herrmann, President TUM, Germany

10:20    Keynote lecture:
Real-time PCR, a personal perspective.
Russell Higuchi,  "Pioneer in real-time PCR”
Associate Director of the Human Genetics Department, Roche Molecular Systems, Almeda, CA, USA

Session:   Pre-Analytical Steps
Chair:   V. Benes
Lecture hall   HS 14

11:00    Session introduction by V. Benes

11:10    mRNA quantification from archival cancer samples.
    Stephen A Bustin, Rebecca Hands, Sina Dorudi, Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, University of London

11:40    Nucleic acid isolation for diagnostic testing using Bayer´s magnetic particles.
Guido Hennig, Bayer HealthCare AG, Diagnostics Research Germany, Leverkusen

12:10     Robust molecular profiling from RNA derived of archival tissue.
Janine Antonov, Departement of Clinical Research, University of Bern, Switzerland.

12:40 – 13:40    Lunch in the student cafeteria

13:40    Nucleic Acid Stabilization in Cultured Cell and Tissue Lysates for QPCR Gene Expression Analysis.
L. Scott Basehore, Sr. Research Associate, Stratagene Research & Development Department

14:10    Standardization of RNA Quality Assessment using the RNA Integrity Number (RIN) and the 2100 bioanalyzer.
Marc Valer, Agilent Technologies, Waldbronn, Germany LIVE presentation of Bioanalyzer 2100

15:00 – 15:30    Coffee break

15:30    Influence of RNA matrix effect on qRT-PCR results – an overview.
Michael W. Pfaffl, Simone Fleige, Physiology, Center of Life Science, Weihenstephan, Technical University of Munich, Germany

16:00    Use of standardized mixtures of internal standards in RT-PCR to generate validated biomarkers and to develop standardized transcript abundance reference databases.
James Willey1, Elizabeth Peters 2, Charles Knight1, Erin Crawford, 1, Bradley Austermiller1, Terry Osborn2;  1: Medical University of Ohio, Toledo, Ohio, United States.  2: Gene Express, Inc., Toledo, Ohio, United States.

16:30    Optimization of reverse transcription for two-step QRT-PCR: A comparison of RT priming methods and the addition of a new enhancer for efficient removal of double-stranded DNA contamination.
Ian Kavanagh1, Stephanie Noel1, Chatu Rajapakshe1, Gerwyn Jones1, Nicky Quispe1, Simon Baker(1, 2), Meg Martel1,  1: ABgene, Epsom, United Kingdom. 2: Birkbeck, University of London, United Kingdom.

17:00 – 18:30    Refreshments in the Industrial Exhibition,   Get-together with the Companies

18:30 – 20:00    Poster - Session

20:00 – 24:00    Poster – Party
                    welcome by Prof. Heinrich H.D. Meyer
                    Salonorchester Karl Edelmann is presenting a variety of international music

                    Poster Party is sponsored by  Roche Applied Science  &  Eppendorf


Tuesday  6th September 2005

Session:   New Application – part 1:  single cells

Chair:   M. Kubista
Lecture hall   HS 14

08:00    Session introduction by M. Kubista

08:10    Gene expression profiling in single cells.
Anders Ståhlberg (1), Martin Bengtsson (1,2), Patrik Rorsman(2,3) and Mikael Kubista (1)
1: Department of Chemistry & Bioscience / Molecular Biotechnology, Chalmers University of Technology and TATAA Biocenter, Sweden.  2: Department of Experimental Medical Science, Lund University, Sweden.  3: The Oxford Centre for Diabetes, Endocrinology and Metabolism, The Churchill Hospital, Oxford, England.

08:40    Quantitative single-cell RT-PCR and calcium imaging in acute brain slices.
Robert Blum, Guylaine M. Durand, Nima Marandi, Simone D. Herberger, Arthur Konnerth,  Ludwig-Maximilians-Universität, Germany.

09:10    Amplification based assays in nanoliter volume range.
Andreas Dahl1, Marc Sultan1, Regine Schwartz1, Matthias Lange1, Alexander Jung2, Michael Steinwand2, Kenneth Livak2, Hans Lehrach1 and Lajos Nyarsik1,  (1) Max Planck Institute for Human Genetics, Deutschland (2) Applied Biosystems.

09:40    Forensic and single-molecule assays of mitochondrial DNA using LATE-PCR.
    Arthur Reis, Lawrence J. Wangh, Brandeis University, Boston, MA, USA

10:10 – 10:40    Coffee break

Session:   New Application - part 2:  new methods

Chair:   R. Higuchi
Lecture hall   HS 14

10:40    Rapid Development of RT-PCR Assays for RNAi Experiments Using Pre-designed LNA-probe Libraries.
    Michael Boutros, Boveri-Group Signaling and Functional Genomics, DKFZ, Heidelberg, Germany.

11:10    A Multiplex Branched DNA Assay for Parallel Quantitative Gene Expression Profiling.
Michael Flagella*, Son Bui*, Zhi Zheng, Cung Tuong Nguyen, Aiguo Zhang, Larry Pastor, Yunqing Ma, Wen Yang, Kim Crawford, Gary K. McMaster, Frank Witney and Yuling Luo, Genospectra Inc., United States.

11:40    TripleHYB: A novel detection format for real-time PCR.
Anne-Katrin Rost 1, Natalia Malchowa 2, Awad A. Osman 1, Thomas Köhler 1,   1: AJ Roboscreen GmbH, Delitzscher Strasse 135, D-04129 Leipzig, Germany.  2: Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland.

12:00    Correlation of microarray and quantitative real-time PCR results.
Elisa Wurmbach, Mount Sinai School of Medicine, United States.

12:30 – 13:30    Lunch in the student cafeteria

Session:   New Application - part 3:  multiplexing

Chair:   S. Bustin
Lecture hall   HS 14

13:30    The best of both worlds - New dyes for qPCR for use in combination with probes.
    Neven Zoric, Coordinator of the TATAA Biocenter, Göteborg, Sweden.

14:00    qPCR pitfalls - primer and probe design / fluorophore quencher combinations.
Clémence Beslin, Eurogentec, Belgium.

14:30    Going MULTI – how to easily achieve high multiplexing in real-time PCR.
    Andreas Missel, Associate Director R&D, QIAGEN GmbH, Hilden

15:00    Plexor™ Real-Time Quantitative PCR Systems: Multiplexed assays made easy.
    Kyle Hooper, Promega Corporation, Woods Hollow Road, Madison, WI, USA

15:30        Two-color multiplex assay for the identification of Orthopoxes viruses with Real-Time LUX PCR.
    Mohamed Aitichou 1, Sandrine Javorschi-Miller 2, Sofi Ibrahim 1, Mark Andersen 2,   1: Virology Division, United States Army Medical Research Institute of Infectious Diseases, United States. 2: Invitrogen, United States.

16:00 – 16:30    Coffee break

Session:   New Application - part 4:  mixed session

Chair:   T. Bar
Lecture hall   HS 14

16:30    ChIP studies on a HOX gene regulated by Polycomb group and trithorax group proteins.
Bernadett Papp, EMBL, Germany.

17:00    The Ups and Downs of Gene Regulation: Validating siRNA Gene Expression Disruption with RT-qPCR.
    Hilary Katherine Srere, Bio-Rad Laboratories, United States.

17:30    Real-time immuno-PCR for quantification of proteins.
Kristina Lind, Mikael Kubista;  Department of Chemistry and Bioscience, Chalmers University,

18:00    microRNA expression profiles from Real-time PCR classify ES and differentiated cells
Simone Guenther 1, Adam Broomer 2, Dana Ridzon 2, Kai Lao2, Karl Guegler2, William Strauss3;   1: Applied Biosystems, Darmstadt, Germany.  2: Applied Biosystems, Foster City,USA.  3: University of Colorado, Boulder, USA.

19:00 – 24:00    Symposium Gala Dinner

Location:  Lindenkeller, Pasta & More, Freising
·    Bavarian Buffet
·    Mediterranean Buffet
·    Asian Buffet
·    Modern Crossover Buffet
·    Music and Dancing

Tuesday  6th September 2005

Session:   Normalization

Chair:   N. Zoric
Lecture hall   HS 15

08:00    Session introduction by N. Zoric

08:10    Normalization of gene expression: state of the art and preview on a new strategy using expressed Alu repeats.
    Jo Vandesompele, Center for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium

08:40    Normalisation of mRNA levels against total DNA content.
    Shu-Rui Li 1, Doug Storts 2, Becky Hands 1, Benjamin Krenke 2, Ethan Strauss 2, William Ogunkolade 1, Stephen Andrew Bustin 1,  1: Queen Mary University of London, United Kingdom. 2: Promega Corporation, USA, Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, University of London

09:10    Normalization genes for heart failure myocardium in mice, rats and humans.
Trond Brattelid (2,3), Lisbeth Winer (1), Ole M. Sejersted (1,3) and Kristin B. Andersson (1,3)
1: Institute for Experimental Medical Research, Ullevaal University Hospital, University of Oslo.   2: Department of Pharmacology, University of Oslo.   3: Center for Heart Failure Research, Faculty of Medicine, University of Oslo.

09:40    Early mouse development and mammalian embryonic stem cells: a qRT-PCR story.
Erik Willems1, Caroline Kemp1, Ileana Mateizel2, Karen Sermon2 and Luc Leyns1,  1. Lab for Cell Genetics, Vrije Universiteit Brussel, Brussels, Belgium.   2: Research Centre for Reproduction and Genetics, Vrije Universiteit Brussel, Brussels, Belgium.

10:10 – 10:40    Coffee break

Session:  Optimisation – part 1

Chair:   B. Rutledge
Lecture hall   HS 15

10:40    Session introduction by b. Rutledge

10:50    Design and optimization of Taqman and SYBR Green I real-time qPCR assays.
Greg Shipley, Director, Quantitative Genomics Core, Laboratory, The University of Texas Health Science Centre- Houston, USA

11:20    Comparison of MMP gene expression analysis by capillary and "realplex" real-time PCR.
    Raimund Kinne, Experimentelle Rheumatologie, Klinikum der Friedrich-Schiller-Universität Jena, Germany

11:50    Finding the needle in the haystack - LNA bases enhance SNP detection dramatically.
Olfert Landt, TIB MOLBIOL Syntheselabor GmbH Eresburgstraße, Berlin, Germany

12:20    Infectious disease diagnostic research in Africa; the role of real time PCR.
    Jim Huggett, Centre for Infectious Diseases & International Health, University College London, UK

12:50 – 13:50    Lunch in the student cafeteria

Session:  Optimization – part 2

Chair:   H.H.D. Meyer
Lecture hall   HS 15

13:50    Relative real time PCR for gene expression measurement in breast cancer biopsies.
A.Larionov1, S.White1, D.B.Evans2, A.Krause2, M.J.Dixon1, W.R.Miller1;  1: Breast Research Group, Western General Hospital, Edinburgh, UK. 2: Novartis Pharma AG, Basel, Switzerland.

14:20    The fitness of a football team: High Resolution Melts for the determination of genotypes.
    Valin Reja1, Brant Bassam1 and Thomas Kaiser2;  1: Corbett Research, Mortlake, NSW, Australia.  2: Corbett Research UK Limited, Cambridge Science Park, Milton, Cambridge, UK.

14:50    Validation of fast PCR protocols with the Eppendorf Mastercycler ep realplex.
Cynthia Potter, Eppendorf UK Limited, Vision Park, Chivers Way, Histon, Cambridge, UK

15:20 – 15:50    Coffee break

Session:  Standardization

Chair:   G. Shipley
Lecture hall   HS 15

15:50    Session introduction by G. Shipley

16:00    A Comparison of Real-Time RT-PCR Technique, Chemistries and Instrumentation in Laboratories Utilizing the Same Assay.
    Pamela Scott Adams, Director, Molecular Biology Core Facility, Trudeau Institute, Saranac Lake, NY, USA

16:30    Accurate Gene Expression Analysis with High Flexibility: Concepts and Developments.
Oliver Geulen, Roche Applied Science, Mannheim, Germany

17:00    The Data Comparability Challenge - Standards and Best Practices.
Morten T. Andersen, Bio-Molecular Innovation, LGC, Teddington, Middlesex, UK

17:30    Putting the "quantity " into quantitative PCR: A simplified approach to the establishment and application of quantitative scale.
Bob Rutledge, Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Sainte-Foy, Quebec, Canada

18:00    Assay standardisation using universal internal controls and lyophilized reagent beads.
Andreas Eckelt, Cepheid SA, Deutschland.

19:00 – 24:00    Symposium Gala Dinner

Location:  Lindenkeller, Pasta & More, Freising
·    Bavarian Buffet
·    Mediterranean Buffet
·    Asian Buffet
·    Modern Crossover Buffet
·    Music and Dancing

Wednesday  7th September 2005

Session:   BioInformatics

Chair:   M. W. Pfaffl
Lecture hall   HS 14

08:00    Session introduction by M. W. Pfaffl

08:10    From Sequences to Synthesis: Optimal Amplification through Careful Oligonucleotide Selection.
Ben Sowers, Research Associate, Biosearch Technologies

08:40    Estimation of sample specific efficiency – methods and applications.
Tzachi Bar, Department of Chemistry and Biosciences Chalmers University of Technology, Göteborg, Sweden;
Ales Tichopad, LabonNet, Kirchheim near Munich, Germany

09:10    Amplification efficiency dynamics and its implications: Developing a kinetic-based approach for quantitative analysis.
    Bob Rutledge, Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Sainte-Foy, Quebec, Canada

09:40    qBase: relative quantification software for management and automated analysis.
Jan Hellemans, Center for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium

10:10 – 10:40    Coffee break

10:40    Early Phase Fluorescence Fitting of real-ime PCR reaction.
Hervé Rhinn, Laboratoire de Pharmacologiqe Chimique et Génétique, France.

11:10    Classification of real-time PCR data.
    Mikael Kubista, Professor of Biotechnology,
MultiD Analyses AB, Göteborg, Sweden

11:40    The real-time PCR primer and probe database RTPrimerDB: a major update.
Filip Pattyn, Piet Robbrecht, Jelle Verspurten, Anne De Paepe, Frank Speleman, Jo Vandesompele,  Center for Medical Genetics Ghent (CMGG), Ghent University Hospital, Ghent, Belgium.

12:10    Closing of the Symposium in HS 14
            Michael W. Pfaffl

Wednesday  7th September 2005

Session:  GMO Analytics & Food Hygiene

Chair:   C. Albrecht
Lecture hall   HS 15

08:00    Session introduction by C. Albrecht

08:10    Keynote lecture:
Uncertainties and certainties in GMO analytics using qPCR.
Philipp Hübner, Kantonales Laboratorium Basel-Stadt, Abteilungsleiter Lebensmittel, Basel, Switzerland.

08:50    Accurate GMO quantification in food samples.
Dörte Wulff, Research and Development, Eurofins Genescan / GeneScan Analytics GmbH, Freiburg, Germany

09:20    The USDA/GIPSA Proficiency Program: A Summary of Participants Capabilities for Detecting and Quantifying Transgenic Events in Corn and Soybeans.
Ron Jenkins, USDA/GIPSA, USA

09:50 - 10:30    Coffee break

10:30    Application of synthetic DNA-standards for the quantitative screening of different genetically modified rapeseed lines via real-time PCR.
Francisco Moreano, Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Infektologie / Molekularbiologie, German

11:00    Cloned plasmid DNA molecules as a tool for GMO analysis.
Isabel Taverniers, Marc De Loose
Department of Plant Genetics and Breeding, DvP-CLO, Melle, Belgium.

11:30    Detection of Food Pathogens using the Smart Cycler II.
Martina Fricker, Dep. of Bioscience, Technical University of Munich, Freising, Germany

Closing of the Symposium
Lecture hall   HS 14

12:10 – 12:20    Closing of the Symposium.
                        Michael W. Pfaffl

12:20 – 13:00    Lunch in the student cafeteria

Symposium & Workshops    agenda
Sessions & Workshops   overview
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