Session
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invited academic Speaker
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preliminary Title
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Keynote speaker
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Russell
Higuchi
"Pioneer
in real-time PCR"
Associate Director
of the Human Genetics Department, Roche Molecular
Systems, Almeda, CA, USA
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Real-time PCR,
a personal perspective.
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Pre-analytical steps
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Stephen
A Bustin
Professor of Molecular Science
Institute of Cell and Molecular Science
Queen Mary's School of Medicine and
Dentistry, University of London
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mRNA quantification from archival cancer samples.
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Normalization
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Stephen
A Bustin
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Normalisation of mRNA levels against total DNA content.
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Bioinformatics
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Mikael
Kubista
Professor of Biotechnology,
MultiD
Analyses AB, Göteborg, Sweden
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Classification
of real-time PCR data.
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New Applications
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Neven
Zoric
Coordinator of the TATAA Biocenter
Göteborg, Sweden
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The best of
both worlds - New dyes for qPCR for use in combination
with probes.
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Pre-analytical steps
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Michael
W. Pfaffl
Reader in
Physiology
Center of Life Science, Weihenstephan
Technical University
of Munich, Germany
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Influence of
RNA matrix effect on qRT-PCR results - an overview.
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Array Verification
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Elisa
Wurmbach
Assistant Professor
Mount Sinai School
of Medicine
Department of Hematology/Oncology
New York, NY, USA
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Correlation
of microarray and quantitative real-time RT-PCR
results.
|
Optimization
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Greg Shipley
Director, Quantitative
Genomics Core
Laboratory, The University
of Texas Health Science Centre- Houston, USA
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Design and optimization of Taqman and SYBR Green
I real-time qPCR assays.
|
Standardization
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Pamela
Scott Adams
Director,
Molecular Biology Core Facility, Trudeau Institute,
Saranac Lake, NY, USA |
A Comparison
of Real-Time RT-PCR Technique, Chemistries and Instrumentation
in Laboratories Utilizing the Same Assay.
|
Normalization
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Jo
Vandesompele
Center for Medical Genetics Ghent
Ghent University
Hospital,
Ghent, Belgium
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Normalization of gene expression: state of the art and preview
on a new strategy using expressed Alu repeats.
|
BioInformatics
|
Jan Hellemans
Center for Medical Genetics Ghent
Ghent University
Hospital, Ghent, Belgium
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qBase: relative quantification software for management
and automated analysis.
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New Applications
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Bernadett Papp
Gene Expression Unit
EMBL, Heidelberg, Germany
|
ChIP experiments
from Drosophila tissues analyzed by qRT-PCR.
|
Optimization
|
Anders Stahlberg
Department
of Chemistry and Biosciences
Chalmers University of Technology
Göteborg,
Sweden
|
Single Cell
Transcriptome.
|
GMO
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Ron Jenkins
USDA/GIPSA
USA
|
The USDA/GIPSA Proficiency Program:
A Summary of Participants Capabilities for Detecting and Quantifying
Transgenic Events in Corn and Soybeans.
|
Keynote speaker
GMO
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Philipp
Hübner
Kantonales Laboratorium Basel-Stadt, Abteilungsleiter
Lebensmittel, Basel, Switzerland
|
Uncertainties
and certainties in GMO analytics using qPCR.
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GMO
|
Isabel Taverniers
Ministry of the Flemish Community,Centre
for Agricultural Research – CLO,Dept. for Plant Genetics
and Breeding – DvP, Belgium
|
Cloned plasmid DNA molecules
as a tool for GMO analysis.
|
GMO
|
Francisco Moreano
Bayerisches Landesamt für Gesundheit
und Lebensmittelsicherheit (LGL), Infektologie / Molekularbiologie,
Germany
|
Application of synthetic DNA-standards
for the quantitative screening of different genetically modified
rapeseed lines via real-time PCR.
|
New
Applications
|
Kristina Lind
Department
of Chemistry and Biosciences
Chalmers University of Technology
Göteborg,
Sweden
|
Real-time immuno-PCR for the
quantification of proteins.
|
Bioinformatics
|
Tzachi
Bar & Ales Tichopad
LabonNet,
Silberhornstrasse 3, Kirchheim bei München, Germany
|
Efficiency
estimation from single amplification curve – methods
and applications.
|
Standardization
|
Bob Rutledge
Natural Resources Canada,
Canadian Forest Service, Laurentian Forestry Centre
Sainte-Foy, Quebec,
Canada |
Putting the
"quantity " into quantitative PCR:
A simplified approach to the establishment
and application of quantitative scale.
|
Bioinformatics
|
Bob Rutledge
|
Amplification efficiency dynamics
and its implications: Developing a kinetic-based approach
for quantitative analysis.
|
Standardization
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Morten
T. Andersen
Bio-Molecular Innovation, LGC
Teddington, Middlesex, UK
|
The Data Comparability
Challenge - Standards and Best Practices.
|
New
Applications
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Jim Huggett
Centre for Infectious Diseases
& International Health, University College London,
UK
|
Infectious disease diagnostic research
in Africa; the role of real time PCR.
|
New
Applications
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Arthur Reis
Brandeis University,
Boston, MA, USA
|
Forensic and Single-Molecule Assays
of Mitochondrial DNA Using LATE-PCR.
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|
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academic Speaker
|
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New
Applications
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Michael Boutros
Boveri-Group Signaling and Functional
Genomics, DKFZ, Heidelberg, Germany
sponsored
by Roche Applied Science
|
Rapid Development of RT-PCR
Assays for RNAi Experiments Using Pre-designed LNA-probe Libraries.
|
Optimization
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Raimund Kinne
Experimentelle Rheumatologie, Klinikum der Friedrich-Schiller-Universität
Jena , Germany
sponsored
by
Eppendorf
|
Comparison of MMP gene expression
analysis by capillary and "realplex" real-time PCR.
|
Food Hygiene
|
Martina Fricker
Dep. of Bioscience, Technical University
of Munich, Freising, Germany
sponsored by
Cepheid
|
Detection of Food Pathogens
using the Smart Cycler II.
|
Pre-analytical steps
|
James
Willey
Medical University of Ohio, TOLEDO, OH 43614, USA
|
Use of standardized
mixtures of internal standards in RT-PCR to generate validated biomarkers
and to develop standardized transcript abundance reference databases.
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industrial R&D Speaker
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Standardization
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Oliver Geulen
Roche Applied Science, Mannheim, Germany
|
Accurate Gene Expression Analysis with
High Flexibility: Concepts and Developments. |
Optimization
|
Cynthia Potter
Eppendorf UK
|
Validation of fast PCR protocols
with the Eppendorf Mastercycler ep realplex.
|
| Pre-analytical steps |
Guido Hennig
Bayer HealthCare AG
Diagnostics Research Germany
Leverkusen
|
Nucleic acid
isolation for diagnostic testing using Bayer´s
magnetic particles.
|
Optimization
|
Olfert Landt
TIB MOLBIOL Syntheselabor
GmbH Eresburgstraße, Berlin, Germany
|
Finding the
needle in the haystack - LNA bases enhance SNP detection dramatically.
|
GMO
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Dörte
Wulff
Research and Development
Eurofins Genescan / GeneScan Analytics
GmbH, Freiburg, Germany |
Accurate GMO
quantification in food samples.
|
New Applications
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Andreas Missel
Associate Director R&D, QIAGEN
GmbH, Hilden
|
Going MULTI – how to easily achieve
high multiplexing in real-time PCR.
|
New
Applications
|
Hilary K. Srere
Bio-Rad Laboratories Europe, Munich,
Germany
|
The Ups and Downs of Gene Regulation:
Validating siRNA Gene Expression Disruption with
RT-qPCR.
|
Pre-analytical
steps
|
L. Scott Basehore
Sr. Research Associate, Stratagene Research
& Development Department
|
Nucleic Acid Stabilization
in Cultured Cell and Tissue Lysates for QPCR Gene Expression Analysis. |
New Applications
|
Kyle Hooper
Promega Corporation, Woods Hollow Road, Madison,
WI, USA
|
Plexor™ Real-Time Quantitative
PCR Systems: Multiplexed assays made easy.
|
Optimization
|
Clemence Beslin
product manager, Eurogentec,
Belgium |
qPCR pitfalls
- primer and probe design / fluorophore quencher combinations.
|
Optimization
|
Thomas Kaiser
Corbett Research, Sydney, Australia
|
The fitness of a football team:
High Resolution Melts for the determination of genotypes.
|
New-Applications
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Mark Andersen
Invitrogen, USA
|
Two-color
multiplex assay for the identification of Orthopoxes viruses
with Real-Time LUX PCR.
|
Transcriptomics
|
Simone Guenther
Applied Biosystems, Applera Deutschland
GmbH, Darmstadt, Germany
|
microRNA expression profiles from
real-time PCR classify ES and differentiated cells.
|
Bioinformatics
|
Ben Sowers
Research Associate, Biosearch
Technologies
|
From Sequences
to Synthesis: Optimal Amplification through Careful Oligonucleotide
Selection.
|
Pre-analytical
steps
|
Marc Valer
Agilent Technologies,
Waldbronn, Germany
|
Standardization
of RNA Quality Assessment using the RNA Integrity Number (RIN)
and the 2100 bioanalyzer.
LIVE presentation
of Bioanalyzer 2100
|
Standardization
|
Andreas
Eckelt
Cepheid SA, Germany |
Assay
standardisation using universal internal controls and lyophilized reagent
beads.
|
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All scientific contributions
will be published in the qPCR 2005 Symposium
Proceedings ISBN
3000166874
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