qPCR 2007 Symposium Talks
Keynote Lecture:
"Pioneer in qPCR"
Thomas W. Myers
Director, Program in Core Research,
Roche Molecular Systems, US
Advances in Quantitative PCR for Research and Diagnostic Applications.

Dr. Myers joined Roche Molecular System’s Program in Core Research as a Research Investigator in 1991, advancing to his current position as Director in 2005.  He is a recognized expert in PCR technology, holding numerous patents in the field and authoring many publications during his distinguished career.  In October 2001, Dr. Myers was the recipient of the first Roche Molecular Systems, Inc. 'President's Award for Innovation' in recognition of his pioneering contributions to establishing Roche's RT/PCR technology.
Prior to joining Roche, Dr. Myers was a postdoctoral fellow with Cetus Corporation, where he worked with David H. Gelfand, Ph.D. in the Department of Core Technology within the PCR Division of the company developing PCR methodologies (1990-1991).  Prior to Cetus Corporation, Dr. Myers held a postdoctoral fellowship with Robert A. Bambara, Ph.D in the Biochemistry Department of the University of Rochester in New York studying the enzymology of mammalian DNA replication (1987-1990).  Dr. Myers received his Ph.D. in Chemistry from Wayne State University in Michigan with Louis J. Romano, Ph.D. investigating proteins involved with the replication of bacteriophage T7 (1981-1987).
Dr. Myers holds memberships with the American Chemical Society, American Society for Biochemistry and Molecular Biology, and the American Society for Microbiology and is a frequent speaker at conferences around the world.

invited academic speaker
preliminary title
Main session:
single-cell RT-qPCR

Birgit Liss
Molecular Neurobiology, Department of Physiology & Pathophysiology, Medical Faculty, Philipps University Marburg, Germany
Quantitative RT-qPCR of individual dopaminergic neurons from vital and fixed  tissues.

Anders Stahlberg
Stem Cell Center
Lund University, Lund, Sweden
Detection and quantification of mRNAS in single human embryonic stem sells.

Sanjay Tyagi
Public Health Research Institute (PHRI) at the International Center for Public Health (ICPH),
Newark, NJ, US
Large Scale Cell-to-cell Variations in Gene Expression.

Benedita Rocha
INSERM U345, Institut Necker,
Paris, France
Quantification of multiple genes expression in single cells.

Salvatore Pece
IEO (Istituto Europeo di Oncologia)
Molecular portraiting of normal and tumor human breast stem cells.

sponsored by:
Applied Biosystems,
Applera Deutschland GmbH, Darmstadt, Germany

Martin Bengtsson
Department of Experimental Medical Science, Lund University, Lund, Sweden
Quantitative PCR of heterogeneous tissue: Lessons from the islets of Langerhans.

Radek Sindelka
Institute of Molecular Genetics, Prague, Czech Republic
Intracellular expression profiles in the Xenopus laevis oocytes revealed by quantitative real-time PCR.

Wolfgang Mann
Vize President, Head of Molecular Biology
Advalytix AG, Brunntahl, Germany
Systematic genetic analysis of single cells by PCR.

Dirk Kemming
of Tumor Biology, Center of Experimental Medicine, University Medical Center  Hamburg Eppendorf, Germany

Quantitative real time PCR for single tumor cell based diagnostics.

sponsored by:
Eppendorf AG, Hamburg, Germany

Main session:
microRNA / siRNA
Gunter Meister
Arbeitsgruppe RNA Biologie, Max Planck Institut für Biochemie, Martinsried, Germany

Functional analysis of microRNA-containing protein complexes in human cells.

Leendert H. J. Looijenga
Professor in Translational Patho-Oncology, Department of Pathology, Erasmus MC-University Medical Center Rotterdam
(Daniel den Hoed Cancer Center)
Rotterdam, The Netherlands
(micro)RNAome of human germ cell tumors: pathological and clinical implications

sponsored by:
Applied Biosystems,
Applera Deutschland GmbH, Darmstadt, Germany

Vladimir Benes
Head of Genomics Core Facility,
EMBL, Heidelberg, Germany

MicroRNA profiling toolbox: points to consider.

Greg Shipley
Director, Quantitative Genomics Core
Laboratory, The University of Texas Health Science Centre- Houston, TX, US
Validation of Hits from an siRNA Library Screen Using Real-Time qPCR.

New diagnostic applications with real-time PCR Stephen A Bustin
Professor of Molecular Science
Institute of Cell and Molecular Science
Queen Mary's School of Medicine and Dentistry, University of London, UK

Analysis of expression signatures associated with microvascular invasion in colorectal cancer.

Udo Reischl
Institut für Medizinische Mikrobiologie und Hygiene - Universität Regensburg, Germany
Real-time PCR in Diagnostic Microbiology - a review on 9 years of R&D in an academic environment.

Claudio Orlando
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Italy
Quantitative DNA Methylation Analysis.

Jim Huggett
Centre for Infectious Diseases & International Health, University College London, UK
qPCR analysis of molecular targets for developing world pathogen diagnosis; a multi-step approach to a multi-step problem.

Helen White
National Genetics Reference Laboratory,
Wessex Regional Genetics,Salisbury District Hospital Odstock, Salisbury, Wiltshire, UK
Applications of High Resolution Melt Analysis for Genetic Diagnostics.

Immuno-qPCR Christof Niemeyer
Universität Dortmund, FB Chemie, Biologisch Chemische Mikrostrukturtechnik Dortmund, Germany
Immuno-qPCR:  Novel Opportunities in Clinical Diagnostics and Research.

Ruelle Virginie
Université de Liège, Centre de Recherche sur les Proteines Prions, Liège, Belgium
Immuno-Real Time-PCR as a sensitive diagnostic tool: case of prion proteins.

Kristina Lind
Department of Chemistry and Biosciences
Chalmers University of Technology, Göteborg, Sweden
Use of immuno-qPCR for quantifying proteins in large-scale TAP-tag collections.

Pamela Pinzani
Clinical Biochemistry Unit
Department of Clinical Physiopathology
University of Florence, Florence, Italy
Simultaneous measurements of mRNA expression and the corresponding protein level in microdissected tissue samples by real-time technology: PSA in normal and tumour tissues as a demonstrative model.

Michael W. Pfaffl
Reader in Physiology, Center of Life Science, Weihenstephan, Technical University of Munich, Freising, Germany
Review:  10 years of qPCR Data analysis, Biostatistics and Bioinformatics. Recent advances and new perspectives.

Jan Hellemans
Center for Medical Genetics Ghent
Ghent University Hospital
, Ghent, Belgium

Advanced and universally applicable models for relative quantification with flexible inter-run calibration and proper error propagation.

Mikael Kubista
Professor of Biotechnology, MultiD Analyses AB, Göteborg, Sweden
Real-time PCR expression profiling.

Tzachi Bar
LabonNet Ltd., Kinarot - Jordan Valley Technological Incubator, Zemach, Jordan Valley, Israel
Multivariate kinetic outlieyer detection.

High Throughput qPCR
Expression profiling
Colin Brenan
Chief Technology Officer, BioTrove Inc., Woburn, MA, USA
Massively Parallel, Nanoliter-scale PCR for High Throughput Genomics.

Jo Vandesompele
Center for Medical Genetics Ghent
Ghent University Hospital
, Ghent, Belgium
It’s a long road to prognostic qPCR profiling in the clinic.

Luigi Warren
Department of Bioengineering, Stanford University, Stanford, CA, US
Towards High-Throughput Single-Cell Expression Analysis.

industrial R&D speaker
preliminary title


Eli Hefner
Bio-Rad Laboratories, Hercules, CA, US
Beta-actin gene silencing vis siRNA and its effects on protein profiles

High Throughput qPCR Thorsten Träger
Senior Scientist R&D, Qiagen GmbH, Hilden, Germany
Speed matters: Fast ways from template to results.
qPCR NOS session Evan Messenger
R&D, Scientist,  Biosearch Technologies, Novato, CA, US
The intricacies of multiplexing revealed through a pathogen detection assay.
High throughput qPCR Oliver Geulen
R & D, Roche Applied Science, Mannheim, Germany
LightCycler 480 Real-Time PCR System: Innovative Solutions for high throughput PCR.
single-cell qPCR Keith Stanley
Centre for Immunology, St Vincents Hospital
Darlinghurst NSW,
Corbett Life Science, Sydney, Australia
Gene expression profiling from picograms of RNA by Multiplexed Tandem PCR.
qPCR NOS session Douglas Storts
Promega Corporation, Woods Hollow Road, Madison, WI, US
Quantitative, multiplexed amplification with the Plexor qPCR and qRT-PCR Systems.
New diagnostic applications with real-time PCR David H. Persing
Executive Vice President, Chief Medical and Technology Officer, Cepheid, Sunnyvale, CA, US
Democratizing Diagnostics: On-demand detection of Drug Resistant Microbes on the GeneXpert.
qPCR NOS session Clemence Beslin
Product Manager qPCR, Eurogentec, Liege Science Park, Seraing, Belgium
Primer and probe design and synthesis Fluorophore - quencher combinations.
High Throughput qPCR Marc Unger
Director R&D, Fluidigm,  7100 Shoreline Court, South San Francisco, CA, US
BioMark System: A Breakthrough Real-time qPCR System for HT Expression Profiling, MicroRNA Analysis, and Single-Cell qPCR.
microRNA / siRNA Mark Landers
Invitrogen Corporation, Faraday Avenue, Carlsbad, CA,  US

Characterization of miRNA expression in hESC lines using NCode SYBR GreenER miRNA qRT-PCR.
qPCR NOS session
Tania Nolan
European Business Development Manager,
Sigma-Genosys,Homefield Road, Haverhill, UK
Quantification of mRNA using Real Time RT-PCR.
siRNA Martin Greiner
Product Manager 2100 Bioanalyzer, Agilent Technologies Inc, Stevens Creek Blvd
Santa Clara , CA, US

A new method for separation and characterization of Small RNA by On-Chip Electrophoresis.
High Throughput qPCR Manfred Souquet
Application Specialist Genome Lab, Beckman Coulter GmbH, Krefeld, Germany

GeXP - a new approach in high-throughput gene expression analysis.
New diagnostic applications with real-time PCR Martin Alan Lee
Enigma Diagnostics Limited, Building 224, Tetricus Science Park, Dstl Porton Down, Salisbury, UK
Fluorogenic Quantitative PCR for Non-laboratory Applications.

All scientific contributions will be published in the qPCR 2007 Symposium Proceedings   ISBN-13:  978-3-00-020385-5

Any changes ???   =>   please contact the scientific organizer Michael W. Pfaffl via    qPCR2007@wzw.tum.de