Keynote Lecture:
"Pioneer in qPCR"
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Thomas W. Myers
Director, Program in Core Research,
Roche Molecular Systems, US
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Advances in
Quantitative PCR for Research and Diagnostic Applications.
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Dr.
Myers joined Roche Molecular System’s Program in Core Research as a
Research Investigator in 1991, advancing to his current position as
Director in 2005. He is a recognized expert in PCR technology,
holding numerous patents in the field and authoring many publications
during his distinguished career. In October 2001, Dr. Myers was
the recipient of the first Roche Molecular Systems, Inc. 'President's
Award for Innovation' in recognition of his pioneering contributions to
establishing Roche's RT/PCR technology.
Prior to joining Roche, Dr. Myers was a postdoctoral fellow with Cetus
Corporation, where he worked with David H. Gelfand, Ph.D. in the
Department of Core Technology within the PCR Division of the company
developing PCR methodologies (1990-1991). Prior to Cetus
Corporation, Dr. Myers held a postdoctoral fellowship with Robert A.
Bambara, Ph.D in the Biochemistry Department of the University of
Rochester in New York studying the enzymology of mammalian DNA
replication (1987-1990). Dr. Myers received his Ph.D. in
Chemistry from Wayne State University in Michigan with Louis J. Romano,
Ph.D. investigating proteins involved with the replication of
bacteriophage T7 (1981-1987).
Dr. Myers holds memberships with the American Chemical Society,
American Society for Biochemistry and Molecular Biology, and the
American Society for Microbiology and is a frequent speaker at
conferences around the world. |
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invited academic speaker
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preliminary title
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Main session:
single-cell RT-qPCR
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Birgit Liss
Molecular Neurobiology, Department of Physiology & Pathophysiology,
Medical Faculty, Philipps University Marburg, Germany |
Quantitative
RT-qPCR of individual dopaminergic neurons from vital and fixed
tissues. |
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Anders Stahlberg
Stem Cell
Center
Lund University, Lund, Sweden |
Detection
and quantification of mRNAS in single human embryonic stem sells. |
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Sanjay Tyagi
Public Health Research Institute (PHRI) at the International Center for
Public Health (ICPH), Newark, NJ, US |
Large
Scale Cell-to-cell Variations in Gene Expression. |
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Benedita Rocha
INSERM U345, Institut Necker,
Paris, France |
Quantification
of multiple genes expression in single cells. |
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Salvatore Pece
IEO (Istituto Europeo di Oncologia)
Milano
Italy |
Molecular
portraiting of normal and tumor human breast stem cells.
sponsored by:
Applied
Biosystems, Applera
Deutschland GmbH, Darmstadt, Germany |
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Martin Bengtsson
Department of Experimental Medical Science, Lund University, Lund,
Sweden |
Quantitative
PCR of heterogeneous tissue: Lessons from the islets of Langerhans. |
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Radek Sindelka
Institute of Molecular Genetics, Prague, Czech Republic
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Intracellular
expression profiles in the Xenopus laevis oocytes
revealed by quantitative real-time PCR. |
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Wolfgang Mann
Vize President, Head of Molecular Biology
Advalytix AG, Brunntahl, Germany |
Systematic
genetic
analysis of
single cells by PCR. |
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Dirk Kemming
Institute of Tumor
Biology, Center of Experimental Medicine, University Medical Center Hamburg Eppendorf, Germany
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Quantitative
real
time PCR for single tumor cell based diagnostics.
sponsored
by:
Eppendorf AG, Hamburg, Germany
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Main session:
microRNA / siRNA |
Gunter Meister
Arbeitsgruppe RNA Biologie, Max Planck Institut für Biochemie,
Martinsried, Germany
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Functional
analysis of microRNA-containing protein complexes in human cells. |
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Leendert H. J. Looijenga
Professor in Translational Patho-Oncology, Department of Pathology,
Erasmus MC-University Medical Center Rotterdam
(Daniel den Hoed Cancer Center)
Rotterdam, The Netherlands
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(micro)RNAome of human germ cell tumors: pathological and
clinical implications
sponsored by:
Applied
Biosystems, Applera
Deutschland GmbH, Darmstadt, Germany |
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Vladimir Benes
Head of Genomics Core Facility,
EMBL, Heidelberg, Germany |
MicroRNA
profiling toolbox: points to consider.
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Greg
Shipley
Director, Quantitative Genomics Core
Laboratory, The University of Texas Health Science Centre- Houston, TX,
US |
Validation of Hits
from an siRNA Library Screen Using Real-Time qPCR. |
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| New diagnostic applications with
real-time PCR |
Stephen A Bustin
Professor of Molecular Science
Institute of Cell and Molecular Science
Queen Mary's School of Medicine and Dentistry, University of London, UK
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Analysis of
expression signatures associated with microvascular invasion in
colorectal cancer. |
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Udo Reischl
Institut für Medizinische Mikrobiologie und Hygiene
- Universität
Regensburg, Germany
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Real-time
PCR in Diagnostic Microbiology - a review on 9 years of R&D in an
academic environment. |
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Claudio
Orlando
Clinical Biochemistry
Unit, Department
of Clinical Physiopathology, University of Florence, Italy |
Quantitative
DNA Methylation Analysis. |
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Jim
Huggett
Centre for Infectious Diseases & International Health, University
College London, UK |
qPCR
analysis of molecular targets for developing
world pathogen diagnosis; a multi-step approach to a multi-step
problem. |
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Helen White
National Genetics Reference Laboratory,
Wessex Regional Genetics,Salisbury
District Hospital Odstock,
Salisbury, Wiltshire,
UK |
Applications
of High Resolution Melt Analysis for Genetic Diagnostics. |
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| Immuno-qPCR |
Christof
Niemeyer
Universität
Dortmund, FB Chemie, Biologisch Chemische
Mikrostrukturtechnik Dortmund, Germany |
Immuno-qPCR:
Novel Opportunities in Clinical Diagnostics and Research.
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Ruelle Virginie
Université de Liège, Centre de
Recherche sur les Proteines Prions, Liège, Belgium |
Immuno-Real
Time-PCR as a sensitive diagnostic tool: case of prion proteins. |
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Kristina Lind
Department
of Chemistry and Biosciences
Chalmers University of Technology, Göteborg, Sweden |
Use
of immuno-qPCR for quantifying proteins in large-scale TAP-tag
collections. |
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Pamela
Pinzani
Clinical Biochemistry
Unit
Department
of Clinical Physiopathology
University of Florence, Florence, Italy |
Simultaneous
measurements of mRNA expression and
the corresponding protein level in microdissected tissue samples by
real-time technology: PSA in normal and tumour tissues as a
demonstrative model. |
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Biostatistics
BioInformatics |
Michael W. Pfaffl
Reader in
Physiology, Center of Life Science, Weihenstephan, Technical University
of Munich, Freising, Germany |
Review:
10 years of qPCR Data analysis, Biostatistics and Bioinformatics.
Recent advances and
new perspectives.
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Jan Hellemans
Center
for Medical Genetics Ghent
Ghent University Hospital, Ghent, Belgium
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Advanced and
universally applicable models for relative quantification with flexible
inter-run calibration and proper error propagation. |
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Mikael Kubista
Professor
of Biotechnology, MultiD
Analyses
AB, Göteborg, Sweden |
Real-time
PCR expression profiling. |
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Tzachi Bar
LabonNet
Ltd.,
Kinarot - Jordan Valley Technological Incubator, Zemach, Jordan Valley,
Israel
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Multivariate
kinetic outlieyer detection.
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High
Throughput qPCR
Expression profiling |
Colin Brenan
Chief Technology Officer, BioTrove Inc., Woburn, MA, USA
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Massively
Parallel, Nanoliter-scale PCR for High Throughput Genomics.
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Jo Vandesompele
Center for Medical Genetics Ghent
Ghent University Hospital, Ghent, Belgium |
It’s a long road
to prognostic qPCR profiling in the clinic.
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Luigi Warren
Department of
Bioengineering, Stanford University, Stanford, CA, US |
Towards
High-Throughput Single-Cell Expression Analysis. |
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industrial R&D speaker
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preliminary title |
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LEAD
SPONSORS:
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siRNA
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Eli
Hefner
Bio-Rad Laboratories, Hercules, CA, US
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Beta-actin
gene silencing vis siRNA and its effects on protein profiles
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GOLD
SPONSORS: |
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| High
Throughput qPCR |
Thorsten Träger
Senior Scientist
R&D, Qiagen GmbH,
Hilden, Germany |
Speed
matters: Fast ways from template to results. |
| qPCR
NOS session |
Evan
Messenger
R&D,
Scientist,
Biosearch
Technologies, Novato, CA, US |
The
intricacies of multiplexing revealed through a pathogen detection assay. |
| High throughput
qPCR |
Oliver Geulen
R & D, Roche
Applied Science, Mannheim, Germany
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LightCycler 480 Real-Time PCR System: Innovative
Solutions for high throughput PCR. |
| single-cell qPCR |
Keith
Stanley
Centre
for Immunology, St Vincents Hospital
Darlinghurst NSW, Corbett Life Science,
Sydney, Australia |
Gene expression
profiling from picograms of RNA by Multiplexed Tandem PCR. |
| qPCR
NOS session |
Douglas
Storts
Promega
Corporation, Woods Hollow
Road, Madison, WI, US
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Quantitative, multiplexed amplification
with the Plexor qPCR and qRT-PCR Systems. |
| New diagnostic applications with
real-time PCR |
David H. Persing
Executive Vice President, Chief Medical and Technology Officer,
Cepheid,
Sunnyvale, CA, US |
Democratizing
Diagnostics: On-demand detection of Drug Resistant Microbes on the
GeneXpert. |
| qPCR
NOS session |
Clemence
Beslin
Product
Manager qPCR,
Eurogentec, , Belgium |
Primer and probe design and
synthesis Fluorophore
- quencher combinations. |
| High
Throughput qPCR |
Marc Unger
Director R&D, Fluidigm,
7100 Shoreline Court, South San Francisco, CA, US
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BioMark
System: A Breakthrough Real-time qPCR System for HT Expression
Profiling, MicroRNA Analysis, and Single-Cell qPCR.
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| microRNA
/ siRNA |
Mark Landers
Invitrogen Corporation, Faraday Avenue, Carlsbad, CA, US
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Characterization
of miRNA expression in hESC lines using NCode SYBR GreenER miRNA
qRT-PCR. |
| qPCR
NOS session |
Tania Nolan
European Business Development Manager,
Sigma-Genosys,Homefield Road, Haverhill,
UK |
Quantification
of mRNA using Real Time RT-PCR. |
| siRNA |
Martin
Greiner
Product Manager 2100
Bioanalyzer, Agilent Technologies Inc, Stevens Creek Blvd
Santa Clara , CA, US
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A
new method for separation and characterization of Small RNA by On-Chip
Electrophoresis. |
| High
Throughput qPCR |
Manfred Souquet
Application Specialist Genome Lab, Beckman Coulter GmbH, Krefeld,
Germany
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GeXP
- a new approach in high-throughput gene expression analysis. |
| New diagnostic applications with
real-time PCR |
Martin Alan Lee
Enigma Diagnostics Limited, Building 224, Tetricus Science Park, Dstl
Porton Down, Salisbury, UK |
Fluorogenic
Quantitative PCR for Non-laboratory Applications. |
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| All scientific
contributions will be published in the qPCR
2007 Symposium Proceedings ISBN-13:
978-3-00-020385-5 |