qPCR Application Workshops

All three workshops are hosted by the TATAA Biocenter Sweden and TATAA Biocenter Germany (www.tataa.com and http://TATAA.gene-quantification.info ). The TATAA qPCR workshop laboratories and seminar rooms are close to the central lecture hall.

The workshops are aimed at giving participants a deep and objective understanding of real-time quantitative PCR and its applications. The courses are intended for persons considering working with qPCR or scientists currently working with qPCR seeking a deeper understanding.

Three different 2-day workshops will be held in parallel at 29th - 30th March at the qPCR 2007 Event:

Workshop topics:

•    Classical qPCR Application  (2-days)      (fully booked)                                   => download flyer
•    qPCR Biostatistics and Expression Profiling  (2-days)                                     => download flyer
•    Sample Preparation (1-day)    &   Immuno-qPCR  (1-day)    (fully booked)     => download flyer

All workshops offer extensive hands-on training by qPCR experts from TATAA Biocenter Team. Leading probe technologies will be demonstrated, common and emerging instrument platforms will be available and advanced primer and probe design programs will be used. Hands-on training will be provided on quantitative gene expression, including design and optimization of both RT and qPCR protocols.
Participating companies are asked to support the workshops with PCR cyclers, qPCR kits, consumables, centrifuges, pipettes etc…. Please contact Michael Pfaffl at the TATAA Biocenter Germany  Michael.Pfaffl@tataa.com  for information and details.

The course covers all aspects in qPCR and is held during 2-days. Each course is app. 50% hands-on and is limited to 30 participants, resulting in very interactive teaching and everybody given the opportunity to try the instrumentation.
After the course participants will be able to plan and perform qPCR experiments themselves, as well as interpret and analyze data.
Detailed course material and full catering (lunch, coffee, soft drinks and snacks) are included in the course fee.

Please register for the symposium and the workshops via CONFTOOL:   https://www.wzw.tum.de/conftool/

Classical qPCR Application  (2-days):     => download flyer

  • Basic Principles of PCR and qPCR
  • Comparison of different detection technologies
  • Applications of qPCR
  • Probe and Primer design
  • Data Analysis
  • Relative Quantification-considerations and limitations
  • Experimental Design
  • Reverse Transcription
  • Extraction methods
  • Multiplex considerations

qPCR Biostatistics and Expression Profiling  (2-days):     => download flyer

This course explains statistics applicable to qPCR and teaches how to use statistics to interpret real-time PCR gene expression data, and classify samples based on real-time PCR expression profiling. Course is based on seminars and computer-based demonstrations. Please bring your own Laptop to the course !   During the Biostatistics Module you will learn:

  • How to calculate mean, standard deviation (of sample and population), coefficient of variation, confidence interval, P-value.
  • How to compare a group of samples with a mean (simple t-test), to compare two groups of samples (unpaired t-test), and group of samples before and after treatment (paired t-test)
  • How to compare three or more groups (one way ANOVA), and groups of samples measured before, during and after treatment (repeated measures ANOVA)
  • How to study the effect of treatment (linear regression)
  • How to compare samples that are not from a Gaussian population (Wilcoxon test, Mann-Whitney test)
  • How to visualize and interpret real-time PCR expression data of many genes in many samples (principal component analysis)
  • How to identify related samples based on real-time PCR expression profiling (Hierarchical clustering)
  • How to find response profiles describing samples studied by real-time PCR expression profiling (self-organizing maps)
  • How to design real-time PCR expression studies (experimental design).

Sample Preparation (on day 1)    &   Immuno-qPCR  (on day 2):     => download flyer

  • Sample Preparation
  • Introduction and overview of sample preparation.
  • Purification from tissue.
  • Quality control of purified DNA/RNA.
  • Quality control of purified material.
  • Advanced methods.
  • Troubleshooting.
  • Discussion and Q&A.
  • Immuno-qPCR
  • Immunoassays.
  • The immuno-qPCR assay.
  • Todays experimental setup.
  • Immuno-qPCR experiment quantifying PSA.
    - Preparation of dilution series.
    - Preincubation of PSA and antibody/DNA-conjugate.
    - Washing of wells.
    - Immobilization of protein and conjugate mix in PCR-plate.
    -Washing of wells.       
    -Adding of PCR mastermix.
  • Optimization of immuno-qPCR.
  • Selected applications of immuno-qPCR.
  • How to analyse immuno-qPCR data.
  • Troubleshooting.
  • Analysis of the performed immuno-qPCR experiment.
  • Discussion & questions.

Please register for the qPCR application workshops via CONFTOOL:   https://www.wzw.tum.de/conftool/

Any questions =>  please contact the organizer Michael Pfaffl