All three
workshops are hosted by the TATAA Biocenter Sweden and TATAA Biocenter
Germany (www.tataa.com
and http://TATAA.gene-quantification.info
). The TATAA qPCR workshop laboratories and seminar rooms are close to
the central lecture hall.
The workshops are
aimed at giving participants a deep and objective understanding of
real-time quantitative PCR and its applications. The courses are
intended for persons considering working with qPCR or scientists
currently working with qPCR seeking a deeper understanding.
Three
different 2-day workshops will be held in parallel at 29th - 30th March
at the qPCR 2007 Event:
All workshops offer extensive hands-on training by qPCR experts from
TATAA Biocenter Team. Leading probe technologies will be demonstrated,
common and emerging instrument platforms will be available and advanced
primer and probe design programs will be used. Hands-on training will
be provided on quantitative gene expression, including design and
optimization of both RT and qPCR protocols.
Participating companies are asked to support the workshops with PCR
cyclers, qPCR kits, consumables, centrifuges, pipettes etc…. Please
contact Michael Pfaffl at the TATAA Biocenter Germany Michael.Pfaffl@tataa.com
for information and details.
The
course covers all aspects in qPCR and is held during 2-days. Each
course is app. 50%
hands-on and is limited to 30 participants,
resulting in very interactive teaching and everybody given the
opportunity to try the instrumentation.
After
the course participants will be able to plan and perform qPCR
experiments themselves, as well as interpret and analyze data. Detailed
course material and full catering (lunch, coffee, soft drinks and
snacks) are included in the course fee.
Relative
Quantification-considerations and limitations
Experimental
Design
Reverse
Transcription
Extraction
methods
Multiplex
considerations
qPCR Biostatistics
and Expression Profiling (2-days):=>
download flyer
This course explains
statistics applicable to qPCR and teaches how to use statistics to
interpret real-time PCR gene expression data, and classify samples
based on real-time PCR expression profiling. Course is based on
seminars
and computer-based demonstrations. Please bring your own Laptop
to the course ! During the Biostatistics Module you will
learn:
How
to calculate mean, standard deviation (of sample and population),
coefficient of variation, confidence interval, P-value.
How
to compare a group of samples with a mean (simple t-test), to
compare two groups of samples (unpaired t-test), and group of samples
before and after treatment (paired t-test)
How
to compare three or more groups (one way ANOVA), and groups of
samples measured before, during and after treatment (repeated measures
ANOVA)
How
to study the effect of treatment (linear regression)
How
to compare samples that are not from a Gaussian population
(Wilcoxon test, Mann-Whitney test)
How
to visualize and interpret real-time PCR expression data of many
genes in many samples (principal component analysis)
How
to identify related samples based on real-time PCR expression
profiling (Hierarchical clustering)
How
to find response profiles describing samples studied by real-time
PCR expression profiling (self-organizing maps)
How
to design real-time PCR expression studies (experimental design).
Sample Preparation
(on day 1) & Immuno-qPCR (on
day 2):=>
download flyer
Sample Preparation
Introduction and
overview of sample preparation.
Purification from
tissue.
Quality control of
purified DNA/RNA.
Quality control of
purified material.
Advanced methods.
Troubleshooting.
Discussion and
Q&A.
Immuno-qPCR
Immunoassays.
The immuno-qPCR assay.
Todays experimental
setup.
Immuno-qPCR
experiment quantifying PSA. -
Preparation of dilution series. -
Preincubation of PSA and antibody/DNA-conjugate. -
Washing of wells. -
Immobilization of protein and conjugate mix in PCR-plate. -Washing
of wells. -Adding
of PCR mastermix. -qPCR.
