qPCR 2009 presentations - TALKS
qPCR 2009 presentations - POSTERS

Link to next year qPCR meeting in Vienna => qPCR 2010 in Vienna
download updated and final online PROCEEDINGS from 19th March 2009
=> qpcr2009-online-proceedings.pdf

Monday  9th March 2009

               HOT TOPIC - KEYNOTE LECTURE

10:30       MIQE- guidelines for publication of qPCR data

Stephen A Bustin1, Vladimir Benes2, Jeremy A Garson3, Jan Hellemans4, Jim Huggett3, Mikael Kubista5, Reinhold Mueller6, Tania Nolan7, Michael W Pfaffl8, Gregory L Shipley9, Jo Vandesompele4 and Carl T Wittwer10

1Barts and the London School of Medicine, UK; 2Genomics Core Facility, EMBL Heidelberg, Germany; 3University College London, UK and UCL Hospitals NHS Foundation Trust, UK; 4Ghent University Hospital, Belgium; 5Institute of Biotechnology AS CR, Czech Republic and TATAA Biocenter, Sweden; 6Sequenom, USA; 7Sigma-Aldrich, UK; 8Technical University Munich, Germany; 9University of Texas Health Science Centre, USA; 10University of Utah USA and ARUP Institute for Clinical and Experimental Pathology, USA; s.a.bustin@qmul.ac.uk


Session                Diagnostic & Molecular Markers (1)

Chair                    J. Huggett  & MW. Pfaffl

Lecture hall         HS 14

11:00       A Multi-Assay Approach to the Study of Cellular Toxicity

Gregory L Shipley

UTHSC-Houston, United States of America; gregory.l.shipley@uth.tmc.edu

11:30       Circulating nucleic acids in melanoma diagnosis

Pamela Pinzani1, Francesca Salvianti1, Roberta Cascella1, Vincenzo De Giorgi2, Daniela Massi3, Mario Pazzagli1 and Claudio Orlando1

1Department of Clinical Physiopathology, University of Florence, Florence, Italy; 2Department of Dermatological Sciences, 3Department of Human Pathology and Oncology, University of Florence, Florence, Italy; p.pinzani@dfc.unifi.it

12:00       Prognostic multigene expression classification of cancer patients: a route for success

Joëlle Vermeulen1, Katleen De Preter1, Filip Pattyn1, Liesbeth Vercruysse1, Nurten Yigit1, Jan Hellemans2, Frank Speleman1 and Jo Vandesompele2

1Center for Medical Genetics, Ghent University Hospital, Belgium; 2Ghent University, Belgium - Biogazelle, Belgium; joke.vandesompele@ugent.be

12:30 – 13:30          Lunch in the student cafeteria


Session               Diagnostic & Molecular Markers  - 

Session part 2

Chair                    S. Bustin & G. Shipley

Lecture hall         HS 14

13:30       A Novel Multiplex, Quantitative Gene Expression Approach for Cancer Biomarker Research

Jim Thorn
Beckman Coulter UK, UK, JTHORN@beckman.com

14:00       The use of nucleic acid amplification tests for research and diagnosis in the developing world.

Jim Francis Huggett1, Clare Green1, Michael Hoelscher2 and Alimuddin Zumla1

1Centre for Infectious Diseases and International Health, University College London, UK; 2Department of Infectious Diseases and Tropical Medicine, Klinikum of the University of Munich, Germany; j.huggett@ucl.ac.uk

14:30       Effective placement of LNA into Q-PCR Probes

Raymond Peterson

Celadon Laboratories, United States of America; acollins@celadonlabs.com

15:00       Expression signatures in IBD classification: A new approach

Petra von Stein

InDex Pharmaceuticals AB, Sweden; petra.stein@indexdiag.com

15:30       Intragraft expression profiles by quantitative PCR in kidney transplant patients reflect variability in the response to anti-rejection treatment with corticosteroids

Niels Rekers1, Ingeborg Bajema2, Kim Zuidwijk3, Marko Mallat3, Natascha Goemaere4, Marian van Groningen2, Cees van Kooten3, Hans de Fijter3, Frans Claas1 and Michael Eikmans1 1Department of Immunohematology and Bloodtransfusion, 2Department of Pathology, 3Department of Nephrology, Leiden University Medical Center, The Netherlands; 4St. Pathan, Rotterdam, The Netherlands; n.v.rekers@lumc.nl

16:00 – 16:30          Coffee break

 

Session               Nobel Prize Laureate Lecture

Chair                    R. Cook & MW. Pfaffl

Lecture hall         HS 14        16:30 – 18:00

Nobel Prize Laureate Kary Mullis:
25th Anniversary of PCR 

Session               High Resolution Melting & Genotyping

Chair                    J. Hellemans & A. Stahlberg

Lecture hall         HS 15

13:00       Applications of HRM curve analysis: strengths and pitfalls.

Kim De Leeneer, Ilse Coene, Bruce Poppe, Anne De Paepe and Kathleen Claes

CMGG, Belgium; kim.deleeneer@Ugent.be

13:30       Using Melt Curve Analyses for Experimental Inquiry

Madeline O’Donoghue, Junko Stephens, Nathalie Koch, Jonathan Wang, Gordon Janaway, Laurel Nelson

Applied Biosystems – part of Life Technologies, Foster City, CA madeline.odonoghue@appliedbiosystems.com

14:00       Probe Based Detection of Genetic Variations - Screening and in-vitro Diagnostics

Olfert Landt

TIB Molbiol GmbH, Germany; olandt@tib-molbiol.de

14:30       Releasing the potential of High Resolution Melting analysis

Rob Powell

PrimerDesign Ltd, United Kingdom; rob@primerdesign.co.uk

15:00       Simultaneous Determination of SNP Genotype and Allelic Copy Number of DME Gene CYP2D6

Adam Broomer, Toni Ceccardi, Kelly Li, Yu Wang, Chunlin Xiao and Caifu Chen

Life Technologies, United States of America; chencx@appliedbiosystems.com

15:30       Haematopoietic Chimerism Analysis after Allogeneic Stem Cell Transplantation.

Rosalind Ganderton1, Kate Parratt2, Deborah Richardson2, Kim Orchard2 and Elizabeth Hodges1

1Department of Molecular Pathology, Southampton University Hospitals NHS Trust, Southampton, United Kingdom; 2Department of Haematology, Southampton University Hospitals NHS Trust, Southampton, United Kingdom; rhg@soton.ac.uk

16:00 – 16:30          Coffee break

Tuesday  10th March 2009

 Session               Diagnostic & Molecular Markers (3)

Chair                    U. Reischl  &  H. Nitschko

Lecture hall         HS 14

8:30         Current applications of real-time PCR technology in diagnostic bacteriology

Udo Reischl

University Hospital of Regensburg, Germany;

udo.reischl@klinik.uni-regensburg.de

9:00         Real-time PCR Applications in the diagnostic of highly pathogenic viruses

Andreas Nitsche

Robert Koch Institute, Germany; nitschea@rki.de

9:25         Multiplex-PCR in clinical virology - benefits and limitations

Hans Nitschko, Helga Mairhofer and Anna-Lena Winkler

Max von Pettenkofer-Institute, Germany; nitschko@mvp.uni-muenchen.de

9:50         Realtime PCR of bioterrorism agents

Dimitrios Frangoulidis and Hermann Meyer

Bundeswehr, Germany; DimitriosFrangoulidis@Bundeswehr.org

10:15 – 10:45          Coffee break

10:45       Trans-renal DNA for infectious disease diagnosis

Clare Green, Jim Huggett and Alimuddin Zumla

Centre for Infectious Diseases & International Health, University College London, United Kingdom; clare.green@ucl.ac.uk

11:10       New probes, same procedure - Improved results. Remove the false negatives and positives.

Ulf Bech Christensen

PentaBase, Denmark; ubc@pentabase.com

11:35       The use of DNA/RNA chimeric primers in qPCR for microbial detection and quantification

Ofer Peleg1, Gad Baneth2, Osnat Eyal2, Jakob Inbar1 and Shimon Harrus2

1Genaphora Ltd, Israel; 2Koret School of Veterinary Medicine, The Hebrew University of Jerusalem; ofer.peleg@gmail.com

12:00       Prevalence and viral load of oncogenic Human Papillomavirus types associated with cervical carcinoma in a population of North Italy

Francesco Broccolo

University of Milan-Bicocca, Italy; francesco.broccolo@unimib.it

12:30 – 13:30          Lunch in the student cafeteri

Session                Diagnostics & Molecular Markers in agricultural & veterinary Science

Chair                    HHD. Meyer  &  U. Busch

Lecture hall         HS 14

13:00       Rapid detection and differentiation of Campylobacter jejuni, C. coli and C. lari in food samples using a quadruplex real-time PCR assay
Anja Mayr1, Johann Bauer2, Diana Thäringen1, Ulrich Busch1 and Ingrid Huber1

1Bavarian Health and Food Safety Authority, Germany; 2Institute of Animal Hygiene, TUM; Ingrid.Huber@lgl.bayern.de

13:25       The use of transcriptomics for biomarker development to trace anabolic hormone functions.

Irmgard Riedmaier, Christiane Becker, Michael W Pfaffl and Heinrich HD Meyer

Technical University Munich, Germany; irmgard.riedmaier@wzw.tum.de

13:50       Development of a highly sensitive and specific assay to detect Staphylococcus aureus in bovine mastitic milk

Hans Ulrich Graber

University of Bern, Switzerland; hans.graber@knp.unibe.ch

14:15       Development of a real-time PCR Method for Detection and Quantification of the Fungal Biocontrol Agent Trichoderma atroviride SC1 in Soil

Federica Savazzini1, Claudia Longa2 and Ilaria Pertot2

1CNR, Istituto di Biologia e Biotecnologia Agraria, Via E. Bassini 15, 20133 Milano, Italy; 2FEM-IASMA, Department of Plant Protection, Via Mach 1, 38010, San Michele all'Adige, TN, Italy; savazzinif@yahoo.com

14:40       Temperature influence on expression of selected genes in Mycobacterium avium subsp. paratuberculosis in milk environment

Radka Pribylova, Kralik Petr, Michal Slany and Ivo Pavlik

Veterinary Research Institute, Czech Republic; slany@vri.cz

15:05       Applying Real-time PCR to Determine Co-dominant Genotypes of Dominant SCAR Markers in Common Bean

George J Vandemark1, Phillip N Miklas1, Deidre Fourie2 and Richard Larsen1

1USDA ARS, United States of America; 2ARC Grain Crops Institute, Potchefstroom, South Africa; george.vandemark@ars.usda.gov

15:30  – 16:00         Coffee break

16:00       Comparison of AOE activities and expression levels in the kidney during the development of hypertension in SHR

S Arunkumar1, SK Lee1, KNS Sirajudeen2 and HJ Singh3

1Department of Physiology, School of Medical Sciences, University Science Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.; 2Department of Chemical Pathology, School of Medical Sciences, University Science Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.; 3Faculty of Medicine, Universiti Technologi MARA, 40450 Shah Alam, Selangor, Malaysia.; vsbarun@yahoo.com 

16:25       A quantitative real-time PCR assay for Ehrlichia ruminantium using pCS20

Helena Steyn

ARC-Onderstepoort Veterinary Institute, South Africa; steynh@arc.agric.za

16:50       Relative gene expression of acid-inducible genes in acid-adapted Escherichia coli O157:H7 during lactoperoxidase and lactic acid challenge

ANGELA PARRY-HANSON1, PIET JOOSTE2 and ELNA BUYS1

1UNIVERSITY OF PRETORIA, South Africa; 2TSHWANE UNIVERSITY OF TECHNOLOGY, South Africa; angiep@tuks.co.za 

17:15       A QUANTITATIVE PCR TECHNIQUE FOR EVALUATION OF ARSENIC MOBILITY IN HEAVY METAL CONTAMINATED SAMPLES

Elena A. Polishchuk, Jie Chen, Vivian Lai and William R. Cullen

University of British Columbia, Canada; elena@chem.ubc.ca

17:40       Use of real-time PCR for detection of bovine herpesvirus-1 in cattle and buffalo frozen semen

Samir Kumar Rana1, Srinivasan Alwar Villuppanoor2, Sri Naga Leela Surendra Kota2, Sriraman Rajan2 and Penchala Narasimha Rao Samyam2

1National Dairy Development Board, C/O Indian Immunologicals Ltd., Gachibowli, Hyderabad 500 032, India; 2Indian Immunologicals Ltd., Gachibowli, Hyderabad 500 032, India; skrana@indimmune.com

18:05       Viability of Mycobacterium avium subsp. Para-tuberculosis as measured by PMA-F57real time qPCR

Petr Kralik and Ivo Pavlik

Veterinary Research Institute, Czech Republic; kralik@vri.cz


Session RNAi:     microRNA – siRNA Applications

Chair                    M. Castoldi & MW. Pfaffl

Lecture hall         HS 15

8:30         miQPCR: A novel approach for expression profiling of mature microRNAs.

Mirco Castoldi

EMBL, Germany; castoldi@embl.de

9:00         An inflammatory microRNA signature in muscle cells - a comparative study of cellular models and technological platforms.

Swanhild Meyer2, Carola Wagner3, Michael W Pfaffl2 and Christian Thirion1,4

1SIRION BIOTECH, Martinsried, Germany; 2Lehrstuhl für Physiologie, TUM München, Freising-Weihenstephan, Germany; 3IMGM Laboratories, Martinsried, Germany; 4Laboratory for molecular Myology, Friedrich-Baur-Institute, Department of neurology LMU Munich, Munich, Germany; Thirion@sirion-biotech.de

9:25         Discovery and Validation of Novel Human MicroRNA Genes by SOLiD(TM) and TaqMan®

Jason H. Halsey

Life Technologies / Applied Biosystems, United States of America; halseyjh@appliedbiosystems.com

9:50         mRNA & microRNA integrity - the key to success

Michael W Pfaffl, Christiane Becker, Andrea Hammerle-Fickinger and Irmgard Riedmaier

TUM, Physiology, Weihenstephan, Germany; michael.pfaffl@wzw.tum.de

10:15       microRNAs - developing new tools for diagnostics - Join forces with IMGM Laboratories to make your miRNA project a success

Carola Wagner

IMGM Laboratories GmbH, Martinsried, Germany; carola.wagner@imgm.com

10:40 – 11:00          Coffee break

11:00       A novel and universal method for microRNA RT-qPCR data normalization

Pieter Mestdagh1, Pieter Van Vlierberghe1, An De Weer1, Frank Speleman1 and Jo Vandesompele2

1Center for Medical Genetics, Ghent University Hospital, Belgium; 2Ghent University, Belgium - Biogazelle, Belgium; joke.vandesompele@ugent.be

11:25       Highly sensitive and specific LNA™-enhanced real-time PCR for microRNA expression analysis

Ditte Andreasen, Nana Jacobsen, Liselotte Kahns, Kim Bundvig Barken, Rolf Søkilde and Peter Mouritzen

Exiqon, Denmark; dia@exiqon.com

11:50       Quantification and Functional Analysis of miRNA in Mammalian Cells

Martin Kreutz

QIAGEN GmbH, Germany; martin.kreutz@qiagen.com

12:15       A Novel Simple and Inexpensive Assay for MicroRNAs Detection

Irit Reichenstein, Zvi Bentwich and Yonat Shemer Avni

Ben-Gurion University of the Negev, Israel; Iritreic@bgu.ac.il

 

12:40 – 13:30          Lunch in the student cafeteria
Session               Single-cell qPCR

Chair                    B. Liss & A. Stahlberg

Lecture hall         HS 15

 

13:30       RT-qPCR of individual dopamine neurons form mouse brains and human post mortem brain sections.

Birgit Liss

Molecular Neurophysiology, Institute for General Physiology, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany.; birgit.liss@uni-ulm.de

14:00       Technical aspects of mRNA quantification in single cells using RT-qPCR

Anders Ståhlberg

Gothenburg University, Sweden; anders.stahlberg@neuro.gu.se

14:25       AmpliGrid and AmpliHyb, a new miniaturized, multiplex qPCR system for single cell analysis

Gordana Cerovic2, Marianna Alunni1, Angelique le Bras2, Régis Melizzi2, Jean-Luc Grabias2, Maxime Rattier2, Martin Kantlehner1, Petra Hartmann1, Wolfgang Mann1 and Claude Weisbuch2

1Olympus Life Science Research Europa, Germany; 2Genewave, Palaiseau (France)

gordana.cerovic@genewave.com

14:50       Post-characterization of cultured pituitary cells using single-cell real-time RT-PCR

Kjetil Hodne, Trude M Haug, Olav Sand and Finn-Arne Weltzien

Dept of Molecular Biosciences, University of Oslo, Norway; kjetil.hodne@imbv.uio.no

15:15       Molecular characterization of circulating tumor cells in large quantities of contaminating leukocytes by a multiplex real-time PCR

ANIETA M SIEUWERTS1, JACO KRAAN2, JOAN BOLT-DE VRIES1, PETRA VAN DER SPOEL2, BIANCA MOSTERT2, JOHN W MARTENS1, JAN W GRATAMA2, STEFAN SLEIJFER2 and JOHN A FOEKENS1

1Department of Medical Oncology, Josephine Nefkens Institute and Cancer Genomics Centre, ERASMUS MC, Netherlands, The; 2Department of Medical Oncology, Daniel den Hoed Cancer Center, ERASMUS MC, Netherlands, The; a.sieuwerts@erasmusmc.nl

15:40       Visualization of Single mRNA Molecules

Fay Wang1, John Flanagan1, Yunqing Ma2, Steve Lai2, Takuro Yaoi2, Son Bui2, Li-chong Wang1, Jennifer Wong1, Nan Su1, Jessie Wu2, Nina Nguyen2, Aiguo Zhang2, Steve Chen1, Frank Witney2, Quan Nguyen2 and Yuling Luo1

1Advanced Cell Diagnostics, Inc., United States of America; 2Affymetrix/Panomics, Inc., United States of America; yluo@acdbio.com

16:05 – 16:30          Coffee break

 


Session               Tutorials in qPCR BioStatistics & BioInformatics

Chair                    J. Vandesompele & A. Forootan

Lecture hall         HS 15

16:30       Easy analysis of qPCR data with state of the art quantification models and comprehensive quality controls using qBasePlus.

Jan Hellemans1,2, Stefaan Derveaux1 and Jo Vandesompele1,2

1Center for Medical Genetics, Ghent University, Belgium; 2Biogazelle, Belgium; Jan.Hellemans@UGent.be

17:00       RefGenes - a new tool to find suitable reference genes for selected experimental conditions

Philip Zimmermann

ETH Zurich, Switzerland; phz@ethz.ch

17:30       Data analysis for gene quantification and expression profiling using GenEx.

Anders Bergkvist

MultiD Analyses AB, Sweden; anders.bergkvist@multid.se

18:00       CAmpER - An open analysis framework for real-time PCR data using single sample amplification efficiency calculation.

Jochen Blom1, Lukas Jelonek1, Jörn Kalinowski2, Christian Rückert2 and Alexander Goesmann1

1Bioinformatics Resource Facility, CeBitTec, Bielefeld University, Germany; 2Institute for Genome Research, CeBiTec, Bielefeld Univeristy, Germany; jblom@cebitec.uni-bielefeld.de

Wednesday  11th March 2009

Session               High-Throughout session

Chair                    J. Hellemans & K. Livak

Lecture hall         HS 14

 

8:30         Moving from qPCR Assays to qPCR Arrays

Kenneth James Livak

Fluidigm Corporation, United States of America; ken.livak@fluidigm.com

9:00         Accurate and Objective Gene Copy Number Profiling using Real-Time PCR

Jan Hellemans2, Barbara D'haene1, Frauke Coppieters1, Steve Lefever1, Filip Pattyn1, Bart Leroy1, Geert Mortier1, Elfride De Baere1 and Jo Vandesompele2

1Center for Medical Genetics, Ghent University Hospital, Belgium; 2Ghent University, Belgium - Biogazelle, Belgium; Jan.Hellemans@UGent.be

9:25         High-Throughput Analysis of Nucleic Acids Using the LightCycler® 1536 qPCR Platform

Thomas Froehlich, Gregor Sagner, Gudrun Tellmann, Christian Weilke and Armin Tgetgel

Roche Diagnostics, Germany; thomas.froehlich@roche.com

9:50         Measurement of Gene Expression by Massively Parallel Nanoliter real-time PCR

Jim White

BioTrove, United States of America; jwhite1@biotrove.com

10:15 – 10:45          Coffee break

 

10:45       QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR

Samuel Arvidsson1,2, Miroslaw Kwasniewski1,2,3, Diego Mauricio Riano-Pachon2 and Bernd Mueller-Roeber1,2

1Potsdam University, Germany; 2Max-Planck Institute for Molecular Plant Physiology, Potsdam, Germany; 3University of Silesia, Katowice, Poland; samuel.arvidsson@uni-potsdam.de

11:10       Oligo Design Across the Mouse Genome

Ben Sowers

Biosearch Technologies, Inc., United States of America; ben@biosearchtech.com

11:35       A novel digital technology for non-enzymatic direct multiplexed measurement of gene expression

Chaybani, Ramin, Gary K. Geiss1, Roger Bumgarner2, Brian Birditt1, Timothy Dahl1, Naeem Dowidar1, Dwayne L. Dunaway1, Perry Fell1, Sean Ferree1, Renee D. George1, Tammy Grogan1, Jeffrey J. James1, Malini Maysuria1, Jeffrey D. Mitton1, Paola Oliveri4, Jennifer L. Osborn3, Tao Peng2, Amber L. Ratcliffe1, Philippa J. Webster1, Eric H. Davidson4 and Leroy Hood5

1NanoString Technologies Inc., Seattle, WA 2Department of Microbiology, , University of Washington, Seattle WA; 3Department of Bioengineering, University Washington, Seattle WA ; 4Division of Biology, California Institute of Technology, Pasadena CA ; 5The Institute of Systems Biology, Seattle WA; ramin@novoptim.com

12:00       Sensitive and high throughput multiplexed immunoassays for biomarker discovery in biobanked samples using proximity ligation assays and qPCR.

Simon Fredriksson

Olink Bioscience, Sweden; simon.fredriksson@olink.com

 

Session               qPCR BioStatistics & BioInformatics

Chair                    M. Kubista & A. Tichopad

Lecture hall         HS 14

13:30       Real-time PCR Expression Profiling - Concept of multiway profiling

Mikael Kubista1,2, Anders Ståhlberg2,3, Jose Manuel Andrade4, Björn Sjögreen5,6, Amin Forootan6 and Anders Bergkvist6 1TATAA Biocenter, Sweden; 2Institute of Biotechnology, Czech Academy of Sciences; Prague 3Gothenburg University, Sweden; 4University La Coruna, Spain; 5MultiD Analyses AB, Sweden; 6Lawrence Livermore Laboratory, USA; mikael.kubista@tataa.com

14:00       Design and analysis of Q-RT-PCR assays for haematological malignancies using mixed effects models

Martin Bøgsted1,2, Charlotte Mandrup1, Anders Petersen1, Steffen Falgreen1,2, Hans Erik Johnsen1, Anne Bukh1 and Karen Dybkær1

1Department of Haematology, Aarhus University Hospital, 2Center for Cardiovascular Research, Aarhus University Hospital; Denmark; martin.boegsted@rn.dk

14:25       Adequate experiment design as the first important step in obtaining valid biological inference with qPCR technique

Ales Tichopad

TUM, Germany; ales@tichopad.de 

14:50       Modeling Real-Time PCR Efficiency and Fluorescent Signal intensity for accurate gene quantification using a single standard

Kaminski Karine1, Jahan Virginie2, Lamoure Claire2, Martineau Pierre3 and Molina Franck1

1CNRS / Bio-Rad, France; 2Bio-Rad, France; 3IRCM INSERM, France; karine.kaminski@sysdiag.cnrs.fr

15:15 – 15:45          Coffee break

15:45       The Delta-TF Method for Real-Time PCR Data Standardization

Denis Rebrikov, Elena Goncharova, German Samatov, Pavel Semenov, Alexander Baluev and Dmitry Trofimov

DNA-Technology, Russian Federation; denis@dna-technology.ru

16:10       Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.

Jan M Ruijter

Academic Medical Centre, Amsterdam, the Netherlands; j.m.ruijter@amc.uva.nl 

16:35       The calculation of real-time PCR ratios by means of Monte Carlo Simulation or high-order Taylor expansion.

Andrej-Nikolai Spiess

University Hospital Hamburg-Eppendorf, Germany; a.spiess@uke.de

17:00       rtprimerdb.org: public qPCR assay database with custom assay quality control and primer design pipeline

Filip Pattyn, Steve Lefever, Frank Speleman and Jo Vandesompele

Ghent Univ. Hospital, Belgium; Filip.Pattyn@UGent.be

Session:               qPCR NOS Session (1)

Normalization & Optimization & Standardization

Chair:                    J. Vandesompele & S. Bustin

Lecture hall         HS 15

8:30         A new qPCR assay for the detection of Clostridium difficile

Stephen A Bustin

Barts and the London School of Medicine, United Kingdom; s.a.bustin@qmul.ac.uk

9:00         RealTime ready – Functionally Tested qPCR Assays for Gene Expression Analysis on the LightCycler® Platform

Ralf P. Mauritz

Roche Diagnostics GmbH, Germany; ralf.mauritz@roche.com

9:25         RDML: structured language and reporting guidelines for real-time PCR data

Steve Lefever1, Jan Hellemans2, Filip Pattyn1, Daniel Przybylski3, Chris Taylor4, René Geurts5, Andreas Untergasser5 and Jo Vandesompele2

1Center for Medical Genetics, Ghent University Hospital, Belgium; 2Ghent University, Belgium - Biogazelle, Belgium; 3Bio-Rad Laboratories, Inc. Hercules, California, USA; 4European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SD, UK; 5Laboratory of Molecular Biology, Department of Plant Science, Wageningen University, The Netherlands; steve.lefever@ugent.be

9:50         ZNA: new high-affinity synthetic oligonucleotides as powerful tools for PCR

Nathalie Lenne1, Valérie Moreau1, Emilie Voirin1, Régis Noir2, Clément Paris1, Mitsuharu Kotera2, Jean-Paul Behr2 and Patrick Erbacher1

1Polyplus-transfection, France; 2Laboratoire de Chimie Génétique, Illkirch,France; nlenne@polyplus-transfection.com

10:15 – 10:45          Coffee break

10:45       Importance of experimental design and sample QC for robust and meaningful QPCR results

Steffen Mueller

Agilent, Germany; steffen.mueller@agilent.com

11:10       Normalization of real-time RT-PCR data using an external RNA control

Stian Ellefsen1, Kåre-Olav Stensløkken2,3, Guro Katrine Sandvik3, Tom Arne Kristensen3 and Göran Erik Nilsson3

1Lillehammer University College, Norway; 2Ullevål University Hospital, Norway; 3Department of Molecular Biosciences, University of Oslo, Norway; stian.ellefsen@hil.no

11:35       Highly Accurate Quantitative Gene Expression Analysis without Use of pre-defined Normalizer Genes using Pattern Recognition Analysis

Dan Shaffer, Volker Vogel and Don-Paul Kovarcik

Daniel J. Shaffer, VP and Founder, Bar Harbor Biotechnology, US, daniel.shaffer@barharborbio.com

12:00       Optimisation and standardisation of sample preparation with the Bead-beating technology in q-PCR analysis.

Romain VEROLLET and Esmeralda CARVALHO

Bertin Technologies, France; verollet@bertin.fr

Session:               qPCR NOS Session (2)

Chair:                    A. Nitsche & A. Stahlberg

Lecture hall         HS 15

13:30       Increasing QPCR throughput: simple steps to speed up results whilst minimising variance.

Gerwyn Jones, Saima Nayab, Srujana Kapavarapu and Ian Kavanagh

Thermo Fisher Scientific, ABgene House, Blenheim Road, Epsom KT19 9AP United Kingdom; ian.kavanagh@thermofisher.com

13:55       Significant difference or artefact of the method? - The impact of temperature performance of real-time thermocyclers on generated qPCR results

Mary Span

CYCLERtest, Netherlands, The; marys@cyclertest.com

14:20       The Next Generation in Hot Start PCR - CleanAmp Primers and dNTPs

Natasha Paul

TriLink BioTechnologies, Inc., United States of America; npaul@trilinkbiotech.com

14:45       Assessment of the reliability of nucleic acid extraction systems commonly used to get valid qPCR results

Tom Øystein Jonassen1, Mona Holberg-Petersen1 and Einar S Berg2

1Ullevål University Hospital, Oslo, Norway; 2Norwegian Institute of Public Health, Oslo, Norway; esbe@fhi.no

15:10 – 15:40          Coffee break

15:40       Housekeeping genes validation in acute and chronic adjuvant arthritic rat for mRNA quantification by real time RT-PCR

Muhammad Ayaz Alam Qureshi, Per Eriksson, Andrea Stark and Mahmood Ahmed

Karolinska Institute, Sweden; alam_ayaz@hotmail.com

16:05       Gene Expression Analysis by Genome Controlled Reverse Transcription-PCR.

Chas Andre1, Jakob Stenman2, Tuomas Tenkanen1, Arto Orpana2 and Susanna Lintula2

1Finnzymes OY, Finland; 2Hospital for Children and Adolescents, Helsinki Finland; jakob.stenman@helsinki.fi

16:30       QPCR Use in Biopharmaceuticals and Current Issues

Chaminda Salgado

NDA Analytics, United Kingdom; chaminda.salgado@nda-analytics.com

 

please direct your enquiry to  Martina.Reiter@bioeps.com   or  qPCR2009@wzw.tum.de    ©  2009