qPCR 2005 - Symposium Talks
qPCR 2005 Symposium Proceedings  ( ISBN 3000166874 )

Keynote speaker
Russell Higuchi
"Pioneer in real-time PCR"
Associate Director of the Human Genetics Department, Roche Molecular Systems, Almeda, CA, USA

Real-time PCR, a personal perspective.
Pre-analytical steps
Stephen A Bustin
Professor of Molecular Science
Institute of Cell and Molecular Science
Queen Mary's School of Medicine and Dentistry, University of London

mRNA quantification from archival cancer samples.

Normalisation of mRNA levels against total DNA content.
Mikael Kubista
Professor of Biotechnology,
MultiD Analyses AB, Göteborg, Sweden
Classification of real-time PCR data.
New Applications
Neven Zoric
Coordinator of the TATAA Biocenter
Göteborg, Sweden

The best of both worlds - New dyes for qPCR for use in combination with probes.
Pre-analytical steps
Michael W. Pfaffl
Reader in Physiology
Center of Life Science, Weihenstephan
Technical University of Munich
, Germany

Influence of RNA matrix effect on qRT-PCR results - an overview.
Array Verification
Elisa Wurmbach
Assistant Professor
Mount Sinai School of Medicine
Department of Hematology/Oncology
New York, NY, USA

Correlation of microarray and quantitative real-time RT-PCR results.
Greg Shipley
Director, Quantitative Genomics Core
Laboratory, The University of Texas Health Science Centre- Houston, USA
Design and optimization of Taqman and SYBR Green I real-time qPCR assays.

Pamela Scott Adams
Director, Molecular Biology Core Facility, Trudeau Institute,
Saranac Lake, NY, USA
A Comparison of Real-Time RT-PCR Technique, Chemistries and Instrumentation in Laboratories Utilizing the Same Assay.
Jo Vandesompele
Center for Medical Genetics Ghent
Ghent University Hospital
, Ghent, Belgium
Normalization of gene expression: state of the art and preview on a new strategy using expressed Alu repeats.
Jan Hellemans
Center for Medical Genetics Ghent
Ghent University Hospital
, Ghent, Belgium

qBase: relative quantification software for management and automated analysis.
New Applications
Bernadett Papp
Gene Expression Unit

EMBL, Heidelberg, Germany
ChIP experiments from Drosophila tissues analyzed by qRT-PCR.
Anders Stahlberg
Department of Chemistry and Biosciences
Chalmers University of Technology
Göteborg, Sweden

Single Cell Transcriptome.
Ron Jenkins
The USDA/GIPSA Proficiency Program: A Summary of Participants Capabilities for Detecting and Quantifying Transgenic Events in Corn and Soybeans.
Keynote speaker
Philipp Hübner
Kantonales Laboratorium Basel-Stadt, Abteilungsleiter Lebensmittel, Basel, Switzerland
Uncertainties and certainties in GMO analytics using qPCR.
Isabel Taverniers
Ministry of the Flemish Community,Centre for Agricultural Research – CLO,Dept. for Plant Genetics and Breeding – DvP, Belgium
Cloned plasmid DNA molecules as a tool for GMO analysis.
Francisco Moreano
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Infektologie / Molekularbiologie, Germany
Application of synthetic DNA-standards for the quantitative screening of different genetically modified rapeseed lines via real-time PCR.
New Applications
Kristina Lind
Department of Chemistry and Biosciences
Chalmers University of Technology
Göteborg, Sweden

Real-time immuno-PCR for the quantification of proteins.
Tzachi Bar & Ales Tichopad
LabonNet, Silberhornstrasse 3, Kirchheim bei München, Germany
Efficiency estimation from single amplification curve – methods and applications.
Bob Rutledge
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre
Sainte-Foy, Quebec, Canada
Putting the "quantity " into quantitative PCR:
A simplified approach to the establishment and application of quantitative scale.

Bob Rutledge
Amplification efficiency dynamics and its implications: Developing a kinetic-based approach for quantitative analysis.
Morten T. Andersen
Bio-Molecular Innovation, LGC
Teddington, Middlesex, UK
The Data Comparability Challenge - Standards and Best Practices.
New Applications
Jim Huggett
Centre for Infectious Diseases & International Health, University College London, UK

Infectious disease diagnostic research in Africa; the role of real time PCR.
New Applications
Arthur Reis
Brandeis University, Boston, MA, USA
Forensic and Single-Molecule Assays of Mitochondrial DNA Using LATE-PCR.


New Applications
Michael Boutros
Boveri-Group Signaling and Functional Genomics, DKFZ, Heidelberg, Germany

sponsored by Roche Applied Science

Rapid Development of RT-PCR Assays for RNAi Experiments Using Pre-designed LNA-probe Libraries.
Raimund Kinne
Experimentelle Rheumatologie, Klinikum der Friedrich-Schiller-Universität Jena , Germany

sponsored by Eppendorf
Comparison of MMP gene expression analysis by capillary and "realplex" real-time PCR.
Food Hygiene
Martina Fricker
Dep. of Bioscience, Technical University of Munich, Freising, Germany
sponsored by Cepheid
Detection of Food Pathogens using the Smart Cycler II.
Pre-analytical steps
James Willey
Medical University of Ohio, TOLEDO, OH 43614, USA
Use of standardized mixtures of internal standards in RT-PCR to generate validated biomarkers and to develop standardized transcript abundance reference databases.


Oliver Geulen
Roche Applied Science
, Mannheim, Germany
Accurate Gene Expression Analysis with High Flexibility: Concepts and Developments.
Cynthia Potter
Eppendorf UK
Validation of fast PCR protocols with the Eppendorf Mastercycler ep realplex.
Pre-analytical steps Guido Hennig
Bayer HealthCare AG
Diagnostics Research Germany
Nucleic acid isolation for diagnostic testing using  Bayer´s magnetic particles.
Olfert Landt
TIB MOLBIOL Syntheselabor GmbH Eresburgstraße, Berlin, Germany
Finding the needle in the haystack - LNA bases enhance SNP detection dramatically.
Dörte Wulff
Research and Development
Eurofins Genescan / GeneScan Analytics GmbH, Freiburg, Germany
Accurate GMO quantification in food samples.
New Applications
Andreas Missel
Associate Director R&D, QIAGEN GmbH, Hilden
Going MULTI – how to easily achieve high multiplexing in real-time PCR.
New Applications
Hilary K. Srere
Bio-Rad Laboratories Europe, Munich, Germany

The Ups and Downs of Gene Regulation:  Validating siRNA Gene Expression Disruption with RT-qPCR.
Pre-analytical steps
L. Scott Basehore
Sr. Research Associate, Stratagene Research & Development Department
Nucleic Acid Stabilization in Cultured Cell and Tissue Lysates for QPCR Gene Expression Analysis.
New Applications
Kyle Hooper
Promega Corporation, Woods Hollow Road, Madison, WI, USA
Plexor™ Real-Time Quantitative PCR Systems: Multiplexed assays made easy.
Clemence Beslin
product manager, Eurogentec, Belgium
qPCR pitfalls - primer and probe design / fluorophore quencher combinations.
Thomas Kaiser
Corbett Research, Sydney, Australia
The fitness of a football team:  High Resolution Melts for the determination of genotypes.
Mark Andersen
Invitrogen, USA
Two-color multiplex assay for the identification of Orthopoxes viruses with Real-Time LUX PCR.
Simone Guenther
Applied Biosystems, Applera Deutschland GmbH, Darmstadt, Germany
microRNA expression profiles from real-time PCR classify ES and differentiated cells.
Ben Sowers
Research Associate, Biosearch Technologies
From Sequences to Synthesis: Optimal Amplification through Careful Oligonucleotide Selection.
Pre-analytical steps
Marc Valer
Agilent Technologies,
Waldbronn, Germany

Standardization of RNA Quality Assessment using the RNA Integrity Number (RIN) and the 2100 bioanalyzer.
Andreas Eckelt
Cepheid SA, Germany
Assay standardisation using universal internal controls and lyophilized reagent beads.
All scientific contributions are published in the qPCR 2005 Symposium Proceedings    ISBN 3000166874