 StepOnePlus Real-Time PCR System: Easy to use for all levels of experience |
 7500 Real-Time PCR System: Trusted performance & dye - versatility |
 ViiA™ 7 Real-Time PCR System: Powerful features & intuitive design |
 QuantStudio 12K Flex Real-Time PCR System: Maximum throughput, flexibility and scalability |
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/ StepOnePlus™ systems |
7500 Fast system | 7500 system | ViiA™ 7 system | QuantStudio™ 12K Flex System | |
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| Price | $ / $$ | $$ | $$ | $$$ | Varies |
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| Colors | 3 / 4 | 5 | 5 | 21 | 21 |
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| Available
blocks |
48 / 96-well | Fast 96-well | 96-well | 96/Fast 96/384-well & TaqMan® Array Card | 96/Fast 96/384-well & TaqMan® Array Card and 3,072 through-hole OpenArray® plate |
| Throughput | / |
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Available
real-time PCR cyclers (1) On this
page the most prominent real-time PCR cycler are described. In the
cycler descriptions the specifications and the advantages of the
displayed systems are shown. Which real-time platform meets your
requirements best, depends on your research application. Some of the
systems are designed for research with low capacities and others are
for high-throughput applications, most in combination with pipetting
robots. Most of them use solid-block for thermal
cycling, other use hot- and cooled-air. Most differences are obviously
in
the application software, especially in the way of data analysis and
how
the derived crossing points or threshold levels are computed. Each of
these
systems employs either one of several general types of fluorescent
probes
for detection. There are also big differences how data are displayed.
Some
of the limitations of end-point detection in (RT-) PCR have been
assuaged in real-time PCR systems, a number of which are now on the
market. These systems offer many general technical advantages,
including reduced probabilities of variability and contamination, as
well as online monitoring and the lack of need for post reaction
analyses. Further, some of these systems were
developed with contemporary applications such as quantitative PCR,
multiplexing,
and high-throughput analysis in mind. Initial template levels can be
calculated by analysing the shape of the curve or by determining when
the signal
rises above some threshold value. A hopefully complete list of the
commercially available
real-time
PCR systems are overviewed on this page and/or summarized below:
ViiA7, OpenArray, StepOne & StepOnePLus 7300, 7500, 7500 FAST, 7900HT Real-Time PCR System www.lifetechnologies.com The StepOne™ Real-Time PCR System combines plug and play convenience and uncompromising performance that is tailor-made for both first-time users and real-time PCR experts. By utilizing the full range of TaqMan® gene expression and genotyping assays, the StepOne System delivers the kind of high-quality results you've come to expect from the leader in real-time PCR. The StepOne System provides the power and flexibility experienced users demand, while its intuitive software guides new users through experimental design, operation, and analysis. Part of an elegant, optimized system solution for real-time PCR that includes software, reagents, and instrumentation, the StepOne System delivers remarkable performance with remarkable ease-of-use, at a price that can only be described as...remarkable.
StepOnePlus™
Real-Time PCR
System
read
more download StepOnePlus brochureThe Applied Biosystems® StepOnePlus™ Real-Time PCR System, makes it simple and easy to get high-quality real-time PCR results. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
Remarkably Simple System The StepOnePlus™ system brings advanced real-time PCR technology to a new level of accessibility. Beginning at the StepOnePlus software homepage, you can seamlessly navigate through all aspects of the real-time PCR method including sample and reaction set-up, thermal cycling, and fluorescent detection. Focused application software analyzes and interprets experimental results. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the Design Wizard. Simply Remarkable Results - Because the StepOnePlus system is factory-calibrated for optical and thermal accuracy, simply remarkable real-time PCR results are available right out of the box. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® Assay with 99.7% confidence. Applications and Software Analysis The StepOnePlus System supports any real-time PCR application.
Instrument software for the Applied Biosystems® StepOne Real-Time PCR System runs on the Windows XP® operating system and provides instrument control, data collection, and data analysis. This user-friendly and intuitive software package includes the following features:
The
7500 Fast Real-Time PCR System
The Applied Biosystems 7500 FAST Real-Time PCR System is a versatile, leading edge platform offering enhanced performance capabilities and a future upgrade to high speed thermal cycling.
The ViiA™ 7 Dx Instrument puts high-productivity qPCR at your fingertips. With the new OptiFlex™ optical system and 4 interchangeable block formats from 96-well to 384-well to TaqMan® Array Micro Fludic Card, the ViiA™ 7 is available for in-vitro diagnostics use. Key product features:
Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 Dx Instrument enhances your lab’s productivity.
The ViiA™ 7 Dx Instrument features the new OptiFlex™ System, which provides enhanced fluorescence detection.
ViiA™ 7 Software ViiA™ 7 software drives value in a number of ways, offering:
The OpenArray Real-Time PCR Instrument and OpenArray AccuFill System combination enables the processing of hundreds to thousands of samples per day enabling mid-density, high-throughput results with ease of use. Key product features:
The OpenArray Real-Time PCR System provides PCR-based genotyping and gene expression analysis of hundreds to thousands of samples and assays per day. Researchers can analyze up to 144 samples on a single OpenArray Plate. Three OpenArray Plates can be cycled and imaged simultaneously on the OpenArray Real-Time PCR Instrument, providing high-throughput results with ease. The system workflow thermal cycles and fluorescently images the OpenArray Plates. Results are captured on the external PC, and the OpenArray Analysis Software enables researchers to view specific assay data. Complete Platform The OpenArray Real-Time PCR Platform includes:
The OpenArray Real-Time PCR Instrument uses three filtered high-brightness LEDs to excite, and a cooled CCD camera to detect fluorescence emission from dyes. The excitation and emission wavelengths support TaqMan FAMdye detection and SYBR Green I, ROX FAMand VIC dye detection, making it an ideal platform for running all TaqMan and SYBR Green Assays. The OpenArray Real-Time PCR Instrument includes an integrated thermal block for cycling. OpenArray real-time PCR Analysis Software OpenArray Real-Time PCR Analysis Software allows you to acquire, analyze and manage data quickly and easily. It presents tabular data and amplification and melt curves for review, while allowing you to adjust analysis parameters. Data from many OpenArray Plates may be combined into the same data project. You can easily modify data with a few mouse clicks to calculate Ct and Tm as well as ΔCt and ΔΔCt measurements. Data can be plotted in a numerical or graphical way, and can be exported into a comma-separated values file (.csv format) for further analysis in many third party analysis packages. OpenArray Genotyping Analysis Software OpenArray Genotyping Analysis Software presents tabular data and automatically-called genotypes for review, while allowing you to adjust analysis parameters. Data from many OpenArray Plates may be combined into the same data project; thousands of data points run at different times can be analyzed on a single scatter plot. You can easily modify calls per assay with a few mouse clicks. Data can be plotted in a numerical or graphical way, and can be exported into a comma separated values file (.csv) for further analysis. Data management and analysis is enabled by TaqMan Genotyper Software, a free SNP genotyping analysis tool that provides a state-of-the-art genotype calling algorithm, an intuitive user interface, enhanced Study-based analysis features, and enables high call rates and accuracy. Combining flexible throughput capabilities with a streamlined workflow, the QuantStudio™ 12K Flex system takes you from targeted discovery through confirmation and screening, all on a single platform. Maximize throughput with minimal resources. Increase your throughput by as much as 32-fold by running 4 OpenArray® plates in a single run on the QuantStudio™ 12K Flex system. It's the equivalent of 32 traditional 384-well qPCR plates or 12,000 data points. Push your throughput even more. When equipped with the OpenArray® block, a single 9700 thermal cycler and the QuantStudio™ 12K Flex OpenArray® AccuFill™ System, the QuantStudio™ 12K Flex system can produce up to 110,000 data points or more in an eight-hour day. For those that need the greatest throughput, add the QuantStudio™ 12K Flex Automation Robot and multiple 9700 thermal cyclers, and you can achieve over 2,000,000 data points in a day. Reduce start up time and hands on time. Start a 12,000 data point experiment typically in 20 minutes or less.
The
LightCycler™ from Roche Molecular Biochemicals of
Indianapolis harnesses light technology licensed from Idaho Technology
Inc. of Idaho Falls, and then adds some additional bells and whistles,
such as a built-in microvolume fluorimetric detection system that
enables real-time quantitative PCR. This system employs thin-walled
glass capillary tubes, and typical PCR experiments can be performed in
less than 30 minutes. Currently, this system supports two
fluorescence-based methods for the detection of amplification products:
the "general" DNA stain SYBR Green I (a product of Molecular Probes of
Eugene, Ore.), or sequence-specific hybridization probe pairs. SYBR
Green I exhibits very little fluorescence when free in solution;
emission is greatly enhanced when it binds to the minor groove of the
DNA double helix. Prior to amplification, the reaction mixture contains
the denatured DNA, the primers, and the dye. The low-level background
fluorescence signal generated by the unbound dye molecules is
subtracted during computer analysis. After annealing of the primers, a
few dye molecules can bind to the double strand. During elongation,
more and more dye molecules bind to the newly synthesized DNA,
resulting in dramatically increased light emission. If the reaction is
monitored continuously, this increase in fluorescence can be viewed in
real time. After denaturation of the DNA during the next heating cycle,
the dye molecules are released and the fluorescence signal falls. A
fluorescence measurement is performed at the end of the elongation step
of every PCR cycle to monitor the increasing amount of amplified DNA.
The hybridization probe format employs two specially designed,
sequence-specificoligonucleotides lab-eled with fluorescent dyes. One
oligonucleotide probe carries a fluorescein label at its 3' end;
the other probe carries a different label (LC Red 640 or LC Red 705) at
its 5' end. The chemical nature of the hybridization probes prevents
their extension: one probe contains fluorescein at the 3' end, whereas
the 5'-labeled probe contains a 3' phosphate moiety. The sequences of
the two oligonucleotides are selected so that they hybridize to the
amplified DNA fragment in a head-to-tail arrangement. When the
oligonucleotides hybridize in this orientation, the two fluorescent
dyes are positioned in close proximity to each other. The first dye
(fluorescein) is excited by the LightCycler's light emitting diode
(LED) filtered light source, and emits green fluorescent light at a
slightly longer wavelength. When the two dyes are in close proximity,
the emitted energy excites the dye attached to the second hybridization
probe, which subsequently emits red fluorescent light at an even longer
wavelength. This energy transfer, referred to as fluorescence resonance
energy transfer (FRET), occurs efficiently only when the dyes are in
close proximity (a distance between 1-5 nucleotides). Thus, in this
type of assay, fluorescent intensity measurements are made after the
annealing steps. The increasing amount of emitted fluorescence is
proportional to the increasing amount of DNA generated during the
linear phase of the ongoing PCR process.
The
LightCycler 480 Real-Time PCR System is a fully integrated
multiwell-plate based real-time PCR platform for highly accurate
qualitative and quantitative detection of nucleic acids. Building on
the benefits of Roche's capillary-based LightCycler® Systems, it
goes one step further in offering enhanced throughput, compatibility
with automation equipment and maximum flexibility regarding hard- and
software.
Providing novel ways to combine speed and accuracy without compromises, the LightCycler® 480 Real-Time PCR System meets the needs of a broad range of applications in research fields such as gene expression studies, discovery and analysis of genetic variation or array data validation. LightCycler® 480 Real-Time PCR System benefits SPEED & ACCURACY
VERSATILITY & CONVENIENCE
COMPATIBILITY & SECURITY
MEET AND EXCEED THE CAPABILITIES OF OTHER REAL-TIME PCR SYSTEMS -TAKE YOUR GENOMIC RESEARCH TO A NEW LEVEL WITH THE LIGHTCYCLER® 480 SYSTEM! Gene Scanning by High Resolution Melting Curve Analysis generally requires the use of:
In a Gene Scanning experiment, sample DNA is first amplified via real-time PCR in the presence of a proprietary saturating DNA dye. A melting curve is then performed using high data acquisition rates, and data are finally analyzed using a Gene Scanning Software, by three basic steps:
 High-resolution melting curve analysis on the LightCycler 480 PCR system (presented by Roche Aplied Science) Roche Applied Science´s LightCycler® family of real-time PCR systems offer fast, accurate and versatile platforms for genetic variation research. The new plate-based LightCycler® 480 System provides the temperature homogeneity and optical characteristics required for high-performance melting-curve analysis (MCA). On the level of data acquisition and available detection channels, this new instrument opens the way to more advanced applications in the emerging field of gene scanning where amplicons can be screened for unknown sequence variations with low efforts in time and cost. The LightCycler® 480 real-time PCR system: a versatile platform for genetic variation research Real-time PCR is a well established technique for studying genetic variation using various probe-based methods for genotyping as well as high-resolution analysis of whole amplicons melted in the presence of saturating DNA dyes. The latter, relatively new, method allows screening for unknown mutations or DNA modifications. The LightCycler® 480 real-time PCR system is a multiwell plate–based instrument that provides integrated applications for detecting and characterizing genetic variation using all these methodological approaches. Transfering PCRs to HRM-assays on the LightCycler 480 System- Examples for BRCA1 High-resolution melting curve analysis (hrMCA) is an attractive technique to scan for unknown mutations in genes. To evaluate how easy or difficult it is to design hrMCA assays using the LightCycler® 480 Instrument, we selected 3 different fragments in exon 11 of the BRCA1 gene, designed an MCA assay, and tested its sensitivity to detect known variants. Rapid high-throughput Methylation analysis using the LightCycler 480 system (presented by Roche Aplied Science) Microsatellite Analysis of Grapevine Varieties by HRM Analysis (by John Mackay) Large-scale, PCR-based gene analysis can only be performed if signals are generated, captured and analyzed in a highly reproducible manner. More precisely, thermal control, optical readout and the algorithms used to characterize genes qualitatively and quantitatively have to be highly precise and reliable. The LightCycler® 1536 System from Roche Applied Science meets these requirements. The novel platform, which utilizes a proprietary 1536-well plate, is capable of performing high-speed, qPCR-based DNA/RNA analyses in an array-like format. High Throughput — Redefined Break through to a new era in high-throughput real-time PCR with the powerful LightCycler® 1536 System, precisely engineered for miniaturization and parallelization.
 LightCycler Nano system - Serious PCR. So much Fun! A small yet complete system with all features you've come to expect from LightCycler® Instruments 1. The LightCycler® Nano Instrument produces high target specificity with high sensitivity characteristic of all LightCycler® Systems. 2. Fast protocols use state-of-the-art, 32-well thermal cycler technology for excellent thermal precision. 3. Robust solid-state optics provide complete spectral information. Learn more about LightCycler® Nano System features Sleek, built to enjoy in every lab, on every bench 1. Small enough for plenty of space on your benchtop. 2. Experience ease-of-use with flexible workflows. 3. Easily install using plug and play. 4. So quiet. You won't know it's running if it wasn't for the light show. 5. Intuitive software from assay setup to analysis. See the LightCycler® Nano System Gallery Great looks meets fast, sensitive and accurate qPCR 1. The LightCycler® Nano System embodies experimental freedom. 2. The instrument uses standard 8-tube PCR strips that come with caps. 3. Flexible reaction volumes permit different levels of sensitivity. 4. Proven reagents for SYBR Green or probe-based assays are available. 5. Use different methods for quantification and mutation analysis, including HRM. 6. Use your preferred operating system, Mac OS or Windows, or go standalone with a USB flash drive. See the LightCycler® Nano System performance data
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Mastercycler® ep realplex - real-time PCR solutions
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