Available real-time RT-PCR cycler and kinetic PCR machines

With the Eco system, Real-Time PCR technology is now within reach of individual researchers. Large, expensive thermal cyclers that take up an entire workspace are replaced with an affordable, compact system and Netbook computer that fit easily in any lab. Delivering unsurpassed data quality for 40-cycle runs in as little as 40 minutes, the Eco system revolutionizes qPCR accessibility for both new and experienced Real-Time PCR users.

The flexible Eco system is an open platform that supports all chemistries and Real-Time PCR applications, including absolute quantification by standard curve, relative quantification using the ΔΔCq method with support for multiple reference gene normalization, allelic discrimination by end-point fluorescence, and gene scanning and genotyping by High Resolution Melt (HRM) curve analysis.

Every Eco system includes a Netbook computer pre-installed with flexible, easy-to-use software that integrates user control, real-time data collection, and advanced data analysis. The software conforms to MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines, making data analysis and submission for publication review more efficient.

Eco software uses a unique icon-driven user interface to simplify experimental design and setup. Pre-set defaults for plate setup and thermal profile are provided for the most commonly used experimental protocols. Temperature and time for each protocol step can easily be changed by click-and-drag action with the mouse. Experiment templates can be customized and saved for future use. All qPCR chemistries and all standard Real-Time PCR applications are supported, with High Resolution Melt (HRM) analysis as a standard option.

The qTOWER, a newcomer on the real-time market, sets new standards for speed.
Based on the established rapidPCR, the qTOWER is up to 10 times faster than commonly available systems, achieving heating rates of 12 °C/sec and cooling rates of 8 °C/sec. Completely quantitative PCR runs can be performed in less than 25 min. The significant reduction of reaction volumes (down to 5 µL) is yet another highlight, as is the exceptional savings (up to 75 %) of expensive real-time reagents.

    * Quantitative real-time fluorescence detection
    * Combines advantages of rapidPCR with enormous sample reduction
    * Heating rates of up to 12 °C/sec and cooling rates of up to 8 °C/sec
    * Low sample consumption down to 5 µl
    * 9 different color modules available, including 4 FRET filters
    * Open for individually adaptations or changes
    * Read-out of 96 wells within 4 sec, independent of the number of dyes
    * Integrated control and analysis software qPCRsoft
    * Real-time PCR and analysis of 96 samples in parallel within less than 60 minutes
    * Variety of methods for data analysis
    * Absolute and relative quantification

The integrated SPS (Sample-Protection-System) also provides optimum sample protection within the thermal block, which is cooled down to 20 °C while the lid heats up to 120 °C prior to starting the actual PCR. The adjustable lid temperature and high contact pressure results in nearly 100 % sample recovery. In addition, condensation effects can also be avoided for small reaction volumes.

Impressive flexibility
The patented fiber-optic system at the heart of qTOWER guarantees detection of homogenous fluorescence signals across the whole microplate. The qTOWER can be equipped with up to four different measuring channels, which makes the device very flexible and adaptable for various applications. The user can choose from nine high-resolution qPCR excitation and emission filters (Color and FRET modules).As a result, the qTOWER is capable of performing ambitious multiplex analyses and covers a broad range of commonly used fluorescence dyes. In addition, the exceptional scan speed of the plate is impressive, because one 96 well microplate will be read out in just four seconds, regardless of the number of colors measured.

qPCRsoft – simple and intuitive
The integrated, intuitive qPCRsoft software serves as the foundation for the final analysis of real-time PCR curves. The program automatically generates different methods for evaluating measured fluorescence data. The program can determine PCR efficiencies and perform absolute and relative quantifications, as well as the delta-delta Ct method and allele discrimination (among other techniques). Researchers can use qPCRsoft to investigate reliable concentrations and precise allele conditions and to display exact expression ratios. Once defined, parameter sets can be applied as templates for future applications and be reused continuously.
Intuitive, exceptionally fast and easy-to-use qPCRsoft controls not only rapid? PCR runs and detects fluorescence signals, it also uses various qPCR methods for evaluating the final data.It follows that qTOWER and the corresponding software combine to form an excellent, highly flexible and exceptional fast real-time rapidPCR system that truly leaves nothing to be desired.

Initiative for energy efficiency
No environmentally hazardous substances, such as lead, mercury, cadmium, hexavalent chromium, PBB or PBDE, were used during the production of qTOWER. The qTOWER also stays ahead of the pack in terms of energy con- sumption. Up to 23 times more efficient than competing models, the qTOWER can dramatically reduce both costs and CO2 emissions.Go green and earth friendly: qTOWER – quantitative real-time rapidPCR.

The most flexible Real-Time PCR Thermocycler

    * Interchangeable block modules
    * Upgrade at existing TProfessional Thermocyclers possible
    * Optionally gradient enabled
    * 6 positions for color filter modules
    * Free customisation with color filter modules
      - definition of the number of filters to be implemented
      - choice amongst 10 different filters (6 fluorescence, 4 FRET)
      - filter combinations covering the whole range of commonly used dyes

    * Patented scanner system with optical fibers
    * Filter-upgrade / interchange at installed units possible
    * Optimal excitation by three long lasting LEDs
    * Easy to use software including commonly used analysis functions

PikoReal Real-Time PCR System is not available in US, Canada, Brazil, UK, Germany, Austria, Switzerland, Italy, Spain, France, Belgium, the Netherlands, Liechtenstein, Denmark and Sweden.

The PikoReal Real-Time PCR System is designed to fit into your lab, even when bench-top space is limited. Offering an exceptionally small footprint this qPCR instrument is ideal for personal use and light enough for field applications. PikoReal also delivers innovative features and outstanding performance, and is part of the complete qPCR workflow offered by Thermo Scientific that includes, sample preparation, nucleic acid extraction, DNA/RNA analysis and qPCR assays and reagents.

48x48 Dynamic Array

96x96 Dynamic Array

Biomark applications:

Fluidigm’s BioMark 96x96 Dynamic Arrays

These new integrated fluidic circuits (IFCs) are capable of performing 9,216 simultaneous experiments without any pre-spotting and with complete flexibility for the researcher. In a word, they are “revolutionary.” Flyer

The 96.96 Dynamic Arrays are at the heart of Fluidigm’s BioMark™ Genetic Analysis System, which delivers new efficiencies in Gene Expression and Genotyping. Fluidigm products enable and accelerate your Single Cell Gene Expression, Copy Number Variation and Absolute Quantitation research in completely new ways.

See how the competition stacks up against the 96x96 dynamic array:

	384-well	96x96 Dynamic Array
Master Mix	46,000µL	240µl
Primer-probe	9,200µL	480µl
Plates	24	1
Pipette steps	18,432	192

*Comparison based on 96 samples tested against 96 assays for a total of 9,216 data points using a single 384-well system running 10ul total reactions and a single BioMark system using the 96.96 Dynamic Array.

The WaferGen SmartChip System enables profiling and validation workflows on a single platform, by combining high-throughput, cost-effective target discovery with the sensitivity, precision, and dynamic range of real-time PCR for validation studies.

Gene Expression Profiling - Discover More
Gene expression profiling of specific diseases has become increasingly important in drug development. Comparison of gene expression patterns between normal and diseased patients or expression profiles in the presence or absence of drugs leads to discovery of genes or a set of genes that can be used in drug development. This requires monitoring of tens, hundreds or thousands of mRNAs in large numbers.
The WaferGen SmartChip System offers the capability to achieve this level of throughput with a high degree of accuracy.

MicroRNA Profiling - Comprehensive human microRNA profile on a single chip
MicroRNAs (miRNA) are post-transcriptional regulators of cell proliferation, tissue differentiation, embryonic development, and apoptosis. Specific miRNA expression profiles may be characteristic of diseases or disease states and used as biomarkers. The identification of miRNA profiles has become important for streamlining drug development processes. The comparison of miRNA patterns from normal and disease samples; or contrasting miRNA profiles of the same sample in the presence or absence of a drug leads to a greater understanding of pathways and mechanisms of action.
The WaferGen SmartChip System in conjunction with the pre-validated SmartChip human miRNA panel, offers researchers the ability to carry out comprehensive, rapid miRNA profiling on their human samples simply, cost-effectively, and accurately.

OpenArray => now with Life Technologies

Imagine performing more than 3,000 assays simultaneously. Imagine generating hundreds of thousands of data points every day. With OpenArray, it’s not just a possibility, it’s a proven fact.

OpenArray represents a major breakthrough for life science researchers, enabling massively parallel, high quality nanoliter assays. It’s ideal for low-volume solution-phase reactions including analysis of genetic, genomic, biochemical, and cellular samples.
Want to find out more? Just follow the links and see how you can put OpenArray technology to work for you.

For more information on specific OpenArray technology products, click on the appropriate link below:

The OpenArray Real-Time qPCR System enables nearly three thousand real-time qPCR assays to be run on a single OpenArray plate
Researchers can test one to 48 different samples against a wide array of assays. The system enables flexibility in experimental design in many research areas:

    * Quantitative expression analysis
    * Pathogen detection
    * Methylation studies
    * Pathway analysis
    * Infectious disease studies
    * Forensics
    * Genetic identification

OpenArray SYBR real-time qPCR

OpenArray® Real-Time qPCR System enables nearly three thousand real-time qPCR assays to be run on a single OpenArray plate. The marriage of the OpenArray technology with the popular SYBR® Green chemistry provides researchers with a high density format that not only saves time but also saves money. With a highly flexible format and nanoliter scale, the OpenArray platform allows for easy adjustment of sample and assay numbers, and enables you to generate more than 24,000 real-time qPCR data points per day, increasing sample throughput while significantly decreasing time and cost.

Fast-Each run on the OpenArray system cycles and images more than 9,000 reactions much higher throughput than plate-based systems. What’s more, in a typical 8-hour workday, one OpenArray system performs multiple runs, helping you zip through your studies.
Flexible-You need to validate microarray results anyway. Now you can use one system for expression profiling, biomarker identification, and screening studies. Plus, the flexible format of the OpenArray system allows you to customize your own gene panel screen 100s of assays or 100s of samples, it’s your choice.
Economical-Only 33nL per reaction! That is 150-fold less than the typical 384-well plate, which allows you to conserve precious sample and reagents. And the system is operated by a single user. That adds up to significant labor and material savings.

BioGene Thermal Cycler Systems

Patent pending Integrated Heating Technology (IHT) forms the basis of this system. It has long been recognised that intra-sample variations caused by thermal gradients within metal block or air-heating systems causes differences in the final product (quantity and quality). IHT uses the concept of directly heating the vessel containing the reaction mix and in addition having a single, infrared temperature sensor for each vessel to ensure truly independent thermal control. Direct heating also has a significant effect on thermal transfer rates as does the ‘capillary’ design of the reaction vessel (allows for a high surface to volume ratio) both leading to Ultra-Rapid thermal cycling. High throughput, cleaner reaction products and the ability to run reactions with different thermal profiles are significant advantages of this technology e.g. running gradients for process optimisation, multiple different tests on the same sample in diagnostic applications where time to report results is critical and high throughput screening. Synchrom mannual synchron.pdf

Patented Electrically Conducting Polymer (ECP) Technology forms the basis of this system. It has long been recognised that intra-sample variations caused by thermal gradients within metal block or air-heating systems causes differences in the final product (quantity and quality).
ECP Technology uses the concept of directly heating the vessel containing the reaction mix and in addition having a single, infrared temperature sensor for each vessel to ensure truly independent thermal control. Direct heating also has a significant effect on thermal transfer rates as does the ‘capillary’ design of the reaction vessel (allows for a high surface to volume ratio) both leading to Ultra Rapid thermal cycling.
High throughput, cleaner reaction products and the ability to run reactions with different thermal profiles are significant advantages of this technology e.g. running gradients for process optimisation, multiple different tests on the same sample in diagnostic applications where time to report results is critical and high throughput screening.
InSyte uses leading edge technology to ensure that the thermal performance is matched by fast, sensitive optics. A compact, high lifetime (10,000 hour) solid-state laser provides an intense, well-collimated pure light source at 473 nm (compatible with all standard chemistries, dyes and dye sets). A multichannel PMT collects all emitted wavelengths between 520 and 720 nm-the system is not constrained by use of filter sets to detect fixed bands of emitted fluorescence. This allows the user to ‘see the full picture’! High scan rates (full plate read every 3 seconds) provide good data sets for use in subsequent analysis.
View InSyte technical document

*	ULTRA-RAPID THERMAL CYCLING
*	UNIQUE 'Electrically Conducting Polymer' (ECP) technology facilitates:
*	DIRECT heating to a specially designed tube to allow rapid thermal equilibration of reaction mix.
*	TRULY Independent temperature control of each tube of the 96-MTP. Choose to run completely different temperature profiles in each tube!
*	FAST SENSITIVE, photon counting optics.
*	COMPREHENSIVE DATA LOGGING/ANALYSES: Embedded Graphical Control System enables user-friendly empirical set up and complete logging/analyses of data.

Real-Time PCR Cycler "DT-322"

Equipment manufactured by "DNA-Technology" corporation

http://www.dna-technology.com/catalog/ob_dt322_en.shtml

Real-Time PCR Cycler "DT-322" is intended for a carrying out of polimerase chain reaction (PCR) with a detection of an accumulation of PCR products during an amplification (real -Time PCR). DT-322 allows to carry out the qualitative and quantitative PCR analysis excepting a stage of the electrophoresis of PCR products in an agarose gel. The device is designed for simultaneous analysis of up to 32 samples. A thermocycler and an optical system of a fluorescence detection are united in this device. All data of the last amplification are recording into a private memory of the PCR Cycler and can be read out even after a current interruption occurred during an amplification. After a restoring of a power supply the amplification program will be continued, and the moment of interruption, its duration and a block temperature in the moment of interruption will be recorded. A turning off or a hanging of external computer does not result in PCR termination since the process control is managed by the private memory of the device that does not depend on a computer.

Brochure and technical parameters (PDF)

TL998 - Real-time Quantitative PCR Detecting System

http://www.medtl.com/english/ckcp.asp?id=8

The TL998 System provides an efficiency and high-speed solution for PCR analysis. Real-time PCR delivers increased sensitivity and a wider dynamic range more rapidly than traditional PCR. TL998 gives you a choice of quantitation methods for gene expression. It is very important in curative evaluation. The TL998 system ideally suited to meet the high-throughput requirements of today's drug discovery process. The TL998 is useful for life science research, and it is innovative and pioneering products for the prevention, diagnosis, evaluation and monitoring of diseases.



		LineGene3310/3320 real-time PCR delection system is up-to-date product based on linegene. the redesigned LineGene3310/3320 recieves the mature design of heating block, control system and detection system, but it was designed more completely and detection system,but it was designde more completely and the heating and cooling rate reached 6C/s. it is more compact and firmly inconstruction.the performance is highly approved,but the apperance becomes smaller and simple. LineGene3310 is mainly recommended to customers in clinic inspection,commercial inspection,blood bank and so on.meanwhile, LineGene3320 is mainly recommended to cusromer im scientific research institute and vadvantage clinic inspection. http://www.bioer.com.cn/E/productsXx.asp?pid=4

		Line-Gene-K is the newest system in Bioer's range of PCR detection systems. It features more advanced implementations of Peltier and fiber optic technology and a new direct current power system. The new instruments share the Peltier effect and fiber optic design features that provide the exceptional accuracy and stability of the Line-Gene instruments.The new units offer improved uniformity,stability and accuracy by utilizing a constant-current power supply and an advanced multi-point temperature control module.The Line-Gene.K has improved temperature control and more uniform heating throughout the sample block to improve the accuracy of PCR detection. The Line-Gene.K series is available in a range of automated and manual models with the ability to handle one, two, three or four combinations of excitation and emission wavelengths. http://www.bioer.com.cn/E/productsXx.asp?pid=3

Line Gene is a complete real-time PCR detection system that can detect and quantitate nucleic acid sequences by monitoring fluorescence value during the PCR process. With innovative design on hardware and software, the unit is an ideal tool for research use in the field of gene analysis. Features: • Long life blue LED light Source • Extremely sensitive PMT detection module • Sandwich thermo-electronic (TE) module • Easy yet powerful software • Data Export http://www.bioer.com.cn/E/productsXx.asp?pid=1

Line Gene II Real-time PCR Detection System is up-to-date product based on Line-gene. By using the advanced optical fiber conducting technique, the unit has strong sensitivity and reliability when detects the fluorescence. At the same time, it inherits all merits of Line-gene so to realize speedy and accurate PCR detection. What's more, the powerful software provides multiple channels fluorescence detection with four kinds of dyes at the same time. And the testing result of samples can be analyzed and compared by groups so that many functions, such as absolute quantitative, comparative quantitative, SNPs and melting curve analysis, can be accomplished. Multiple Applications: TaqMan probe; SYBR Green I; Molecular beacons, FAM, HEX, TAMRA, TEXASRED, ROX and so on. http://www.bioer.com.cn/E/productsXx.asp?pid=2

ExicyclerTM 96 Real-Time Quantitative Thermal Block

http://eng.bioneer.com/bioneer.html?!products/Exicycler.html

ExicyclerTM Real Time Thermal Block and its operation software is optimized for applications such as quantification of gene expression, genotyping, counting bacterial/viral loads, melt-curve analysis and validation of microarray results. Exicycler is also capable of measuring mutual interactions between two molecules, such as protein-protein and protein-ligand, using fluorescence resonance energy transfer (FRET). In addition, the sensitivity, accuracy and system integration-ready design of Exicycler makes it ideal for high-throughput analysis.

http://eng.bioneer.com/bioneer.html?%21Diagnostics/DianosticIns_Exicycler.html