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The Reference in qPCR  -  Academic & Industrial Information Platform
The Gene Quantification page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & RT-qPCR. It presents a lot of new and innovative qPCR applications, chemistries, methods, algorithms, MIQE and QC strategies, cyclers, kits, dyes, analysis methods, events, and services involved. Commercial and academic institutions can present their qPCR tools right here on our qPCR platform. Please browse our page directory

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News & MIQE & APPs

Evolution / Online translation / ASIA portalMIQE - press review ... UPDATE / statistics
InfoPortal & Daily NEWS

Protocols / Methods / Databases / Alets / Books / eBooks / Forums qPCR groups 
qPCR & Webinars
real-time PCR / interesting reviews / TALKs Webinars / Podcast / MultiMedia
Quantification strategy
Quantification strategies in RT-qPCR:  absolute Quantification & relative Quantification
Normalisation

RT-qPCR normalisation strategies & microRNA - Genorm  BestKeeper  RefGenes  NEW
Optimisation ... UPDATE
Optimisation of reaction setup & qPCR procedure / MIQE / Primer-Recources & Algorithms
Bioinformatics & Downloads
data analysis  qBase  qPCR algorithms clustering qPCR statistics LinRegPCR 
NEW software downloads!

REST ... main page  REST - 2009  RefGenes  GenEx 5 ...NEW VERSION
RNA interference

siRNA  saRNA  microRNA & microRNA normalisation   mirtron  NEW

qPCR Events


qPCR 2011  qPCR 2012 in South Korea  qPCR 2010
qPCR Array  &  digital PCR

qPCR array applications, data analysis & normalisation, analysis software  digital PCR
Cyclers  &  Robots
real-time PCR hardware:  Cyclers  ... UPDATE  / qPCR robotics
RNA & RT  &  RNA QC
reverse transcription / mRNA transcript analysis  / RNA QC & RNA / DNA integrity

Dyes  & HRM dyes


detection dyes, probes, and chemistries in real-time PCR / HRM dyes
Molecular Physiology
CNA  HRM  CNV  microRNA  siRNA saRNA RNAi  digital PCR ... UPDATE single-cell qPCR 
qPCR Efficiency
determination of real-time qPCR efficiency by various methods ... new papers !
Chips  Lab-on-Chip
verification of qRT-PCR via cDNA array and microarray Lab-on-Chip PCR & qPCR
qPCR applications
RT-qPCR applications

circulating nucleic acids = CNA  single-cell handling  single-cell qPCR  HRM  digital PCR ... UPDATE
microRNA   Copy Number Variants  RNA interference  siRNA  small activating RNA (saRNA)



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 Gene
Quantification
Newsletter

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qPCR platform participating companies:
qPCR platform participating companies in alphabetical order
Logo Roche Applied Science
Roche_Applied_Science.gene-quantification.info
Roche Applied Science - PCR Workflow System
Speed up your workflow to spend less time pipetting and more time advancing your research

Eurogentec logo
Eurogentec.gene-quantification.info
Eurogentec - Your one-stop-shop Real-Time PCR supplier
logo Qiagen
Qiagen.gene-quantification.info QIAGEN - Fast, real-time PCR on any cycler
logo Bio-Rad
Bio-Rad.gene-quantification.info
Bio-Rad Laboratories - A complete solution.  A focused approach.
logo Life Technologies
LifeTechnologies.gene-quantification.info Life Technologies - Advanced gene expression service provider
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eurofinsdna.gene-quantification.info
Eurofins MWG Operon - High quality DNA probes specific, fast and reliable




Lonza.gene-quantification.info Lonza - Gene Expression Arrays

TIB-Molbiol - Our business is DNA.
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Biosearchtech.gene-quantification.info
Biosearch Technologies - Advancing Nucleic Acid Technologies
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LabonNet.gene-quantification.info
LabonNet - Quality assessment for real-time PCR
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Bioline.gene-quantification.info
Bioline – The PCR Company

TATAA.gene-quantification.info TATAA Biocenter - Express your genius with qPCR
logo MultiD
multid.gene-quantification.info
MultiD Analysis - multidimensional data analysis
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amplifa.gene-quantification.info amplifa - Eco - High-end Real-time PCR Cycler

Biogazelle.gene-quantification.info Biogazelle - accelerating your analysis
logo Idahotech Inc.
idahotech.gene-quantification.info
Idaho Technology Inc. - designed for Hi-Res Melting

PrimerDesign.gene-quantification.info
PrimerDesign - Driving technology, Empowering researchers
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IDTdna.gene-quantification.info Integrated DNA Technologies - PrimeTime® qPCR Assays and Probes for 5′ Nuclease Assays


Thermoscientific.gene-quantification.info
Thermo Scientific - nucleic acid amplification
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TaKaRa.gene-quantification.info Takara Bio - Real-time PCR and RT-PCR reagents designed for accurate quantification and detection






Your want to join the qPCR platform
contact us via   qPCRplatform@gene-quantification.info

qPCR INFO PORTAL



Cooperating  Institutions  &  Laboratories:


With the three main pillars training, event organisation, and project oriented research, BioEPS is covering the whole range of customer interests within the field of life science, focused on in molecular diagnostics and PCR technology. BioEPS offers qPCR application workshops and advanced specialized trainings. Here you will find more information => workshops.gene-quantification.info
BioEPS is located in the start-up center IZB in Freising - Weihenstephan, next to the Munich airport and the capital city of Bavaria, Munich.

Our latest events
  =>  www.qPCR2011.net  and  www.qPCR2010-Vienna.net

TATAA Biocenter is a research provider that offers training and commissioned services within molecular diagnostics and gene expression analysis using real-time PCR and other molecular techniques to quantify nucleic acids. Our competence is based on knowledge and experience accumulated through years of research at leading European Universities. Our offer comprises the entire field of real-time PCR services, including commissioned research, hands-on training, and custom design of real-time PCR assays.
Further activities:  
1)  Open and tailor-made hands-on training courses;    2)  Commissioned research and development;   3)  Custom design of qPCR assays and assay validation;   4)  Product development          Express your genius with qPCR
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qPCRforum.com : Discuss GenEx and qPCR
Welcome to qPCRforum - Forum for discussion about GenEx and qPCR (quantitative real-time PCR)

GenEx software -
GenEx offers advanced methods to analyze real-time qPCR data with simple clicks of the mouse. The methods are suitable to select and validate reference genes, classify samples, group genes, monitor time dependent processes and much more.
Barts and the London

Stephen Bustin is working at the Queen Mary's School of Medicine and Dentistry, www.icms.qmul.ac.uk at the Institute of Cell and Molecular Science.     www.sabustin.org


Department of Gene Expression
Mikael Kubista´s
lab is at the Institute of Molecular Genetics AS CR (http://www.img.cas.cz) and his group is developing real-time PCR assays for different applications such as routine clinical diagnostic and methods for high through put measurements.    TATAA Biocenter Prague

GeneCore is the in-house genomics service center at EMBL for DNA sequencing, next-generation-sequecing, S.A.G.E., microarrays and real-Time qPCR. Services are provided on non-commercial basis for the EMBL community and academic institutions in the context of scientific collaborations within the EMBL member states. Tight links to industry provide state-of-the-art equipment. The experiments and their results feed directly back into improvements of instruments and technologies, spawning the next generation of scientific questions and technologies.


Biogazelle is the real-time PCR data-analysis company, founded in 2007 as a Ghent University spin-off company by Jo Vandesompele and Jan Hellemans. Its founders have more than 10 years of experience in real-time PCR experiment design, assay development and data-analysis. They wrote one of the most influential papers on normalization of gene expression and on data-analysis (together cited more than 2000 times).
Biogazelle's flagship product qbasePLUS is the most powerful, flexible, and user-friendly real-time PCR data-analysis software based on the proven geNorm and qBase technology, enhanced with proprietary algorithms and time-saving features. qbasePLUS is truly accelerating your research.



geNorm VBA applet for Microsoft Excel determines the most stable reference genes from a set of tested genes and calculates a gene expression normalization factor based on the geometric mean of a user-defined number of reference genes.
qBase is a collection of macros for Microsoft Excel
for the management and automated analysis of real-time quantitative PCR data. The program employs a delta-Ct relative quantification model with PCR efficiency correction and multiple reference gene normalization.





qPCR Platform Media Partner:

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Technology Networks were established over 10 years ago with the aim of providing informative websites for life science professionals.
From our UK headquarters near Sudbury, in Suffolk, we run, update and provide an outstanding web-based information solution for those working within the Life Science and Drug Discovery community.
Since its humble beginnings running just one website based on Combinatorial Chemistry, Technology Networks has expanded its portfolio to include over 30 communities. All of which are available through  TechnologyNetworks.com
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GeneQuan Daily NEWS - or daily appearing newsletter of  Gene-Quantification.info web portal - the reference in real time PCR.
An academic and industrial information platform around gene quantification and expression profiling.
Powerd by  Paper.li



Scientist Solutions - Your International Life Science Form          by Scientists.....for Scientists
Scientist Forum, Scientists Discussion Group, International Life Science Board, Forums, Resource, ....etc.
The ScientistSolutions board provides a discussion forum for professionals in the life sciences, where researchers can ask and answer questions, exchange protocols, and post jobs.  www.scientistsolutions.com


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www.Gene-Quantification.info is edited by Michael W. Pfaffl


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